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Molecular bases characterizing rods and cones

Research Project

Project/Area Number 20370060
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics
Research InstitutionOsaka University

Principal Investigator

KAWAMURA Satoru  大阪大学, 大学院・生命機能研究科, 教授 (80138122)

Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥20,410,000 (Direct Cost: ¥15,700,000、Indirect Cost: ¥4,710,000)
Fiscal Year 2010: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2009: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2008: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Keywords視細胞 / 桿体 / 錐体 / 光応答 / 光感度 / 時間分解能 / 光-電位変換機構 / トランスデューシン / ホスホジエステラーゼ / レチナール / cGMP / グアニレートシクラーゼ / レチノイド / 明順応
Research Abstract

Our visual sensation relies on two types of photoreceptors, rods and cones. Rods are highly light-sensitive so that they mediate night vision. Cones are less light-sensitive, and they mediate daylight vision. Rods and cones are different in many aspects. One of the differences is seen in the light response characteristics. Due to this difference, our way of seeing is different at night and daylight. In the present study, we examined the molecular bases of the differences between rods and cones.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (67 results)

All 2010 2009 2008 Other

All Journal Article (18 results) (of which Peer Reviewed: 17 results) Presentation (42 results) Book (1 results) Remarks (6 results)

  • [Journal Article] Larger inhibition of visual pigment kinase in cones than in rods2010

    • Author(s)
      Arinobu, D., Tachibanaki, S. and Kawamura, S.
    • Journal Title

      J. Neurochem

      Volume: 115(1) Pages: 259-268

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Dicalcin inhibits fertilization through its binding to a glycoprotein in the egg envelope in Xenopus laevis2010

    • Author(s)
      Miwa, N., Ogawa, M., Shinmyo, Y., Hiraoka, Y., Takamatsu, K. and Kawamura S.
    • Journal Title

      J. Biol. Chem

      Volume: 285(20) Pages: 15627-15636

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] GRKによる視物質のリン酸化とその制御2010

    • Author(s)
      河村悟
    • Journal Title

      医学のあゆみ

      Volume: 233(9) Pages: 893-898

    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Journal Article] Larger inhibition of visual pigment kinase in cones than in rods2010

    • Author(s)
      Daisuke Arinobu
    • Journal Title

      Journal of Neurochemistry

      Volume: 115 Pages: 259-268

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dicalcin inhibits fertilization through its binding to a glycoprotein in the egg envelope in Xenopus laevis2010

    • Author(s)
      Naofumi Miwa
    • Journal Title

      Journal of Biological Chemistry

      Volume: 285 Pages: 15627-15636

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] High cGMP synthetic activity in carp cones2009

    • Author(s)
      Takemoto, N., Tachibanaki, S., and Kawamura, S.
    • Journal Title

      Proc. Natl. Acad. Sci. USA

      Volume: 106(28) Pages: 11788-11793

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] High cGMP synthetic activity in carp cones.2009

    • Author(s)
      Norihiko Takemoto
    • Journal Title

      Proceedings of the National Academy of Sciences, USA

      Volume: 106 Pages: 11788-11793

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Highly efficient retinal metabolism in cones2008

    • Author(s)
      Miyazono, S., Shimauchi-Matsukawa, Y., Tachibanaki, S., and Kawamura, S.
    • Journal Title

      Proc. Natl. Acad. Sci. USA

      Volume: 105(41) Pages: 16051-16056

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Amino Acid Residues in GRK1/ GRK7 Responsible for Interaction with S-modulin/ Recoverin2008

    • Author(s)
      Torisawa, A., Arinobu, D, Tachibanaki, S. and Kawamura, S.
    • Journal Title

      Photochem. Photobiol

      Volume: 84(4) Pages: 823-830

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of differentially expressed mRNAs in carp rods and cones2008

    • Author(s)
      Shimauchi-Matsukawa, Y., Aman, Y., Tachibanaki, S., and Kawamura, S.
    • Journal Title

      Mol. Vis

      Volume: 14 Pages: 358-369

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] S100-Annexin Complexes : Biology of conditional association2008

    • Author(s)
      Miwa, N., Uebi, T., and Kawamura, S.
    • Journal Title

      FEBS J.

      Volume: 275(20) Pages: 4945-4955

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Rod and cone photoreceptors : molecular basis of the difference in their physiology2008

    • Author(s)
      Kawamura, S. and Tachibanaki, S.
    • Journal Title

      Comp. Biochem. Physiol. Part A

      Volume: 150(4) Pages: 369-377

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Highly efficient retinal metabolism in cones.2008

    • Author(s)
      Miyazono, S.
    • Journal Title

      Proc. Natl. Acad. Sci. USA. 105

      Pages: 16051-16056

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Amino Acid Residues in GRK1/GRK7 Responsible forInteraction with S-modulin/Recoverin.2008

    • Author(s)
      Torisawa, A.
    • Journal Title

      Photochem. Photobiol. 84

      Pages: 823-830

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of differentially expressed mRNAs in carp rodsand cones.2008

    • Author(s)
      Shimauchi-Matsukawa, Y.
    • Journal Title

      Mol. Vis. 14

      Pages: 358-369

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] SlOO-Annexin Complexes : Biology of conditional association.2008

    • Author(s)
      Miwa, N.
    • Journal Title

      FEBS J. 275

      Pages: 4945-4955

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rod and cone photoreceptors : molecular basis of the differencein their physiology.2008

    • Author(s)
      Kawamura, S.
    • Journal Title

      Comp. Biochem. Physiol. Part A 150

      Pages: 369-377

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 昼間視の分子レカニズム2008

    • Author(s)
      橘木修志
    • Journal Title

      生物物理 48

      Pages: 102-107

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] 2種類の視物質キナーゼ(GRK1、GRK7)の活性の違いをもたらす領域の解析2010

    • Author(s)
      瀬野亜希・竹本訓彦・Lalida Sirianant・橘木修志・河村 悟
    • Organizer
      第81回日本動物学会大会
    • Place of Presentation
      東京大学(東京都)
    • Year and Date
      2010-09-23
    • Related Report
      2010 Final Research Report
  • [Presentation] 2種類の視物質キナーゼ(GRK1、GRK7)の活性の違いをもたらす領域の解析2010

    • Author(s)
      瀬野亜希
    • Organizer
      第81回日本動物学会大会
    • Place of Presentation
      東京大学教養学部(東京都)
    • Year and Date
      2010-09-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] Molecular mechanisms determining photoresponse characteristics in rod and cone photoreceptor cells2010

    • Author(s)
      Shuji Tachibanaki、Satoru Kawamura
    • Organizer
      第48回日本生物物理学会年会
    • Place of Presentation
      東北大学(仙台市)
    • Year and Date
      2010-09-21
    • Related Report
      2010 Final Research Report
  • [Presentation] Molecular mechanisms determining photoresponse characteristics in rod and cone photoreceptor cells2010

    • Author(s)
      Shuji Tachibanaki
    • Organizer
      第48回日本生物物理学会年会
    • Place of Presentation
      東北大学川内北キャンパス(仙台市)
    • Year and Date
      2010-09-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] 錐体における高い視物質リン酸化調節活性2010

    • Author(s)
      有信大輔・橘木修志・河村悟
    • Organizer
      第14回視覚科学フォーラム
    • Place of Presentation
      筑波大学(つくば市)
    • Year and Date
      2010-08-26
    • Related Report
      2010 Final Research Report
  • [Presentation] 錐体における高い視物質リン酸化調節活性2010

    • Author(s)
      有信大輔
    • Organizer
      第14回視覚科学フォーラム
    • Place of Presentation
      筑波大学大学会館(つくば市)
    • Year and Date
      2010-08-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] 錐体と桿体における視物質リン酸化とその制御機構2010

    • Author(s)
      有信大輔
    • Organizer
      第16回日本光生物学協会年会
    • Place of Presentation
      大阪大学銀杏会館(吹田市)
    • Year and Date
      2010-08-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] Rod and cone photoreceptors : How they differ in detecting light and maintaining the ability to evoke light responses2009

    • Author(s)
      有信大輔・橘木修志・河村悟
    • Organizer
      The 10th AEARU International Molecular and Biotechnology Workshop
    • Place of Presentation
      National Taiwan University(Taipei, Taiwan)
    • Year and Date
      2009-11-13
    • Related Report
      2010 Final Research Report
  • [Presentation] Rod and cone photoreceptors : How they differ in detecting light and maintaining the ability to evoke light responses2009

    • Author(s)
      Satoru Kawamura
    • Organizer
      The 10th AEARU International Molecular and Biotechnology Workshop
    • Place of Presentation
      Taiwan National University, Taipei
    • Year and Date
      2009-11-13
    • Related Report
      2009 Annual Research Report
  • [Presentation] Identification of a region responsible for higher phosphorylation activity of GRK7 than GRK12009

    • Author(s)
      Aki Seno, Norihiko Takemoto, Shuji Tachibanaki, Satoru Kawamura
    • Organizer
      第47回日本生物物理学会年会
    • Place of Presentation
      アスティ徳島(徳島市)
    • Year and Date
      2009-10-31
    • Related Report
      2010 Final Research Report
  • [Presentation] Identification of a region responsible for higher phosphorylation activity of GRK7 than GRK12009

    • Author(s)
      Aki Seno
    • Organizer
      第47回日本生物物理学会年会
    • Place of Presentation
      アスティ徳島(徳島)
    • Year and Date
      2009-10-31
    • Related Report
      2009 Annual Research Report
  • [Presentation] セブラフィッシュ錐体の主要なタンパク質ES1のノックダウン解析2009

    • Author(s)
      増田隆昌・和田恭高・河村悟
    • Organizer
      第80回日本動物学会大会
    • Place of Presentation
      静岡県コンベンションアーツセンターグランシップ(静岡市)
    • Year and Date
      2009-09-19
    • Related Report
      2010 Final Research Report
  • [Presentation] コイ錐体視細胞における高いcGMP合成活性2009

    • Author(s)
      竹本訓彦・橘木修志・河村悟
    • Organizer
      第80回日本動物学会大会
    • Place of Presentation
      静岡県コンベンションアーツセンターグランシップ(静岡市)
    • Year and Date
      2009-09-19
    • Related Report
      2010 Final Research Report
  • [Presentation] コイ錐体視細胞における高いcGMP合成活性2009

    • Author(s)
      竹本訓彦
    • Organizer
      第80回日本動物学会大会
    • Place of Presentation
      静岡県コンベンションアーツセンター(静岡)
    • Year and Date
      2009-09-19
    • Related Report
      2009 Annual Research Report
  • [Presentation] セブラフィッシュ錐体の主要なタンパク質ES1のノックダウン解析2009

    • Author(s)
      増田隆昌
    • Organizer
      第80回日本動物学会大会
    • Place of Presentation
      静岡県コンベンションアーツセンター(静岡)
    • Year and Date
      2009-09-19
    • Related Report
      2009 Annual Research Report
  • [Presentation] セブラフィッシュ桿体の光応答特性を調節するオプシンキナーゼGRK2009

    • Author(s)
      白木知也
    • Organizer
      第80回日本動物学会大会
    • Place of Presentation
      静岡県コンベンションアーツセンター(静岡)
    • Year and Date
      2009-09-17
    • Related Report
      2009 Annual Research Report
  • [Presentation] 錐体視細胞でのトランスデューシンとPDEの活性化と不活性化の効率2009

    • Author(s)
      橘木修志・越谷祐貴・河村悟
    • Organizer
      第13回視覚科学フォーラム
    • Place of Presentation
      鹿児島大学(鹿児島市)
    • Year and Date
      2009-09-03
    • Related Report
      2010 Final Research Report
  • [Presentation] 錐体視細胞でのトランスデューシンとPDEの活性化と不活性化の効率2009

    • Author(s)
      橘木修志
    • Organizer
      第13回視覚科学フォーラム
    • Place of Presentation
      鹿児島大学(鹿児島)
    • Year and Date
      2009-09-03
    • Related Report
      2009 Annual Research Report
  • [Presentation] GRK1とGRK7の活性の違いをもたらす領域の特定2009

    • Author(s)
      瀬野亜希・竹本訓彦・橘木修志・河村悟
    • Organizer
      第15回日本光生物学協会年会
    • Place of Presentation
      岡崎コンファレンスセンター(岡崎市)
    • Year and Date
      2009-08-20
    • Related Report
      2010 Final Research Report
  • [Presentation] GRK1とGRK7の活性の違いをもたらす領域の特定2009

    • Author(s)
      瀬野亜希
    • Organizer
      第15回 日本光生物学協会年会
    • Place of Presentation
      自然科学研究機構(岡崎)
    • Year and Date
      2009-08-20
    • Related Report
      2009 Annual Research Report
  • [Presentation] High cGMP Synthetic Activity in Carp Cones2009

    • Author(s)
      Norihiko Takemoto, Shuji Tachibanaki, Satoru Kawamura
    • Organizer
      FASEB Summer Research Conferences : The Biology and Chemistry of Vision
    • Place of Presentation
      Peter Bedford Ballroom(Snowmass, Colorado, USA)
    • Year and Date
      2009-06-15
    • Related Report
      2010 Final Research Report
  • [Presentation] Highly Effective Retinal Metabolism in Carp Cones2009

    • Author(s)
      Sadaharu Miyazono, Shin-ya Sato, Shuji Tachibanaki, Satoru Kawamura
    • Organizer
      FASEB Summer Research Conferences : The Biology and Chemistry of Vision
    • Place of Presentation
      Peter Bedford Ballroom(Snowmass, Colorado, USA)
    • Year and Date
      2009-06-15
    • Related Report
      2010 Final Research Report
  • [Presentation] Highly Effective Retinal Metabolism in Carp Cones.2009

    • Author(s)
      Sadaharu Miyazono
    • Organizer
      FASEB Summer Research Conferences : The Biology and Chemistry of Vision
    • Place of Presentation
      Snowmass, Colorado, USA
    • Year and Date
      2009-06-15
    • Related Report
      2009 Annual Research Report
  • [Presentation] High cMP Synthetic Activity in Carp Cones.2009

    • Author(s)
      Norihiko Takemoto
    • Organizer
      FASEB Summer Research Conferences : The Biology and Chemistry of Vision
    • Place of Presentation
      Snowmass, Colorado, USA
    • Year and Date
      2009-06-15
    • Related Report
      2009 Annual Research Report
  • [Presentation] 薄明視と昼間視を特徴づける視細胞の分子メカニズム2008

    • Author(s)
      河村 悟
    • Organizer
      第81回日本生化学会 年会
    • Place of Presentation
      神戸市
    • Year and Date
      2008-12-11
    • Related Report
      2008 Annual Research Report
  • [Presentation] モジュリン、s26によるGRK1/ GRK7制御機構2008

    • Author(s)
      有信大輔・橘木修志・河村悟
    • Organizer
      第46回日本生物物理学会
    • Place of Presentation
      福岡国際会議場(福岡市)
    • Year and Date
      2008-12-04
    • Related Report
      2010 Final Research Report
  • [Presentation] コイ錐体膜起こるレチナール・レチノール酸化還元共役反応2008

    • Author(s)
      佐藤慎哉・宮園貞治・橘木修志・河村悟
    • Organizer
      第46回日本生物物理学会
    • Place of Presentation
      福岡国際会議場(福岡市)
    • Year and Date
      2008-12-04
    • Related Report
      2010 Final Research Report
  • [Presentation] 錐体における高いcGMP合成活性の生理的意義2008

    • Author(s)
      竹本訓彦・松川淑恵・橘木修志・河村悟
    • Organizer
      第46回日本生物物理学会
    • Place of Presentation
      福岡国際会議場(福岡市)
    • Year and Date
      2008-12-04
    • Related Report
      2010 Final Research Report
  • [Presentation] 錐体特異的なGT活性化と不活性化をもたらす分子基盤2008

    • Author(s)
      橘木修志・越谷祐貴・河村悟
    • Organizer
      第46回日本生物物理学会
    • Place of Presentation
      福岡国際会議場(福岡市)
    • Year and Date
      2008-12-04
    • Related Report
      2010 Final Research Report
  • [Presentation] 錐体特異的なGT活性化と不活性化をもたらす分子基盤2008

    • Author(s)
      橘木修志
    • Organizer
      第46回日本生物物理学会 年会
    • Place of Presentation
      福岡市
    • Year and Date
      2008-12-04
    • Related Report
      2008 Annual Research Report
  • [Presentation] 錐体における高いcGMP合成活性の生理的意義2008

    • Author(s)
      竹本訓彦
    • Organizer
      第46回日本生物物理学会 年会
    • Place of Presentation
      福岡市
    • Year and Date
      2008-12-04
    • Related Report
      2008 Annual Research Report
  • [Presentation] コイ錐体膜起こるレチナール・レチノール酸化還元共役反応2008

    • Author(s)
      佐藤慎哉
    • Organizer
      第46回日本生物物理学会 年会
    • Place of Presentation
      福岡市
    • Year and Date
      2008-12-04
    • Related Report
      2008 Annual Research Report
  • [Presentation] S-モジュリン、s26によるGRK1/GRK7制御機構2008

    • Author(s)
      有信大輔
    • Organizer
      第46回日本生物物理学会 年会
    • Place of Presentation
      福岡市
    • Year and Date
      2008-12-04
    • Related Report
      2008 Annual Research Report
  • [Presentation] 薄明視と昼間視を特徴づける視細胞の分子メカニズム2008

    • Author(s)
      河村悟
    • Organizer
      第81回日本生化学会年会
    • Place of Presentation
      神戸ポートピアホテル(神戸市)
    • Year and Date
      2008-12-01
    • Related Report
      2010 Final Research Report
  • [Presentation] コイ錐体視細胞における高いレチノイド代謝活性2008

    • Author(s)
      宮園貞治・松川淑恵・橘木修志・河村悟
    • Organizer
      第79回日本動物学会大会
    • Place of Presentation
      福岡大学(福岡市)
    • Year and Date
      2008-09-06
    • Related Report
      2010 Final Research Report
  • [Presentation] コイ錐体視細胞における高いレチノイド代謝活性2008

    • Author(s)
      宮園貞治
    • Organizer
      第79回日本動物学会大会
    • Place of Presentation
      福岡市
    • Year and Date
      2008-09-06
    • Related Report
      2008 Annual Research Report
  • [Presentation] 桿体と錐体の光受容機構の違いの分子メカニズム2008

    • Author(s)
      河村悟
    • Organizer
      第79回日本動物学会大会
    • Place of Presentation
      福岡大学(福岡市)
    • Year and Date
      2008-09-05
    • Related Report
      2010 Final Research Report
  • [Presentation] 悍体と錐体の光受容機構の違いの分子メカニズム2008

    • Author(s)
      河村 悟
    • Organizer
      第79回日本動物学会大会
    • Place of Presentation
      福岡市
    • Year and Date
      2008-09-05
    • Related Report
      2008 Annual Research Report
  • [Presentation] 桿体・錐体の応答の違いを生み出す分子機構2008

    • Author(s)
      橘木修志・松川淑恵・有信大輔・宮園貞治・竹本訓彦・越谷祐貴・瀬野亜希・河村悟
    • Organizer
      第12回視覚科学フォーラム
    • Place of Presentation
      大阪大学(豊中市)
    • Year and Date
      2008-08-28
    • Related Report
      2010 Final Research Report
  • [Presentation] 桿体・錐体の応答の違いを生み出す分子機構2008

    • Author(s)
      橘木修志
    • Organizer
      第12回視覚科学フォーラム
    • Place of Presentation
      豊中市
    • Year and Date
      2008-08-28
    • Related Report
      2008 Annual Research Report
  • [Presentation] 大阪大学蛋白質研究所セミナー「生物における光情報変換の一般性と多様性」2008

    • Author(s)
      河村悟・橘木修志・有信大輔・竹本訓彦
    • Organizer
      錐体電位発現機構
    • Place of Presentation
      大阪大学蛋白質研究所(吹田市)
    • Year and Date
      2008-04-19
    • Related Report
      2010 Final Research Report
  • [Presentation] 錐体電位発現機構2008

    • Author(s)
      河村 悟
    • Organizer
      大阪大学蛋白質研究所セミナ-「生物における光情報変換の一般性と多様性」
    • Place of Presentation
      吹田市
    • Year and Date
      2008-04-19
    • Related Report
      2008 Annual Research Report
  • [Book] 視覚の光生物学2010

    • Author(s)
      河村悟
    • Total Pages
      212
    • Publisher
      朝倉書店
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Remarks] ホームページ

    • URL

      http://www.bio.sci.osaka-u.ac.jp/~kawamura/index2.htm

    • Related Report
      2010 Final Research Report
  • [Remarks] 研究教育用ビデオ:-網膜での視物質の褪色と再生

    • URL

      http://www.youtube.com/watch?v=lfVW2vlCabk

    • Related Report
      2010 Final Research Report
  • [Remarks] 研究教育用ビデオ:-桿体膜での視物質の褪色と再生

    • URL

      http://www.youtube.com/watch?v=Ufks_8uBouc

    • Related Report
      2010 Final Research Report
  • [Remarks] 研究教育用ビデオ:-吸引電極法と桿体折り取り標本の作成法

    • URL

      http://www.youtube.com/watch?v=doYiOloRHQA

    • Related Report
      2010 Final Research Report
  • [Remarks] 研究教育用ビデオ:-明所と暗所とでの色の見え方の違い

    • URL

      http://www.youtube.com/watch?v=i4U37Uws4Fs

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.bio.sci.osaka-u.ac.jp/~kawamura/index2.htm

    • Related Report
      2010 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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