Structural study of proteasome by single particle analysis

• Project/Area Number: 20370065
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biophysics
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: MITSUOKA Kaoru 独立行政法人産業技術総合研究所, バイオメディシナル情報研究センター, 研究チーム長 (60301230)
• Co-Investigator(Kenkyū-buntansha): YASUNAGA Takuo 九州工業大学, 大学院・情報工学研究院生命情報工学系, 教授 (60251394) ; SAEKI Yasushi 公益財団法人東京都医学総合研究所, 生体分子先端研究分野, 主席研究員 (80462779)
• Project Period (FY): 2008 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000); Fiscal Year 2012: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2010: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2009: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2008: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 構造生物学 / プロテアソーム / 単粒子解析 / 電子顕微鏡 / 三次元再構成 / 画像解析 / 酵母

Research Abstract

The proteasome is a protein complex, which serves to degrade proteins, and is a protease in the major cytosolic proteolytic system with critical functions in cell cycle control, transcription, signal transduction, inflammation and many

Research Products

• [Journal Article] Processing of electron diffraction patterns with the XDP program (2013)
  • Author(s): Mitsuoka, K.
  • Journal Title: Methods in Molecular Biology Volume: 955 Pages: 539-550
  • DOI: 10.1007/978-1-62703-176-9_29
  • ISBN: 9781627031752, 9781627031769
• [Journal Article] Structural basis for specific recognition of Rpt1p, an ATPase subunit of the 26S proteasome, by a proteasome-dedicated chaperone Hsm3p (2012)
  • Author(s): Takagi, T., et al
  • Journal Title: Journl of Biological Chemistry Volume: 287 Issue: 15 Pages: 12172-12182
  • DOI: 10.1074/jbc.m112.345876
  • Peer Reviewed
• [Journal Article] Obtaining high-resolution images of biological macromolecules by using a cryo-electron microscope with a liquid-helium cooled stage (2011)
  • Author(s): Mitsuoka, K
  • Journal Title: Micron Volume: vol. 42 Issue: 2 Pages: 100-106
  • DOI: 10.1016/j.micron.2010.08.006
  • Peer Reviewed
• [Journal Article] The catalytic activity of Ubp6 enhances maturation of the proteasomal regulatory particle (2011)
  • Author(s): Sakata, E., Stengel, F., Fukunaga, K., et al
  • Journal Title: Molecular Cell Volume: 42 Issue: 5 Pages: 637-649
  • DOI: 10.1016/j.molcel.2011.04.021
  • Peer Reviewed
• [Journal Article] Obtaining high-resolution images of biological macromolecules by using acryo-electron microscope with a liquid-helium cooled stage. (2011)
  • Author(s): Mitsuoka, K.
  • Journal Title: Micron 42 Pages: 100-106
  • Peer Reviewed
• [Journal Article] Obtaining high-resolution images of biological macromolecules by using a cryo-electron microscope with a liquid-helium cooled stage. (2011)
  • Author(s): Mitsuoka K.
  • Journal Title: Micron Volume: 42 Pages: 100-106
  • Peer Reviewed
• [Journal Article] Helical image reconstruction of the outward-open human erythrocyte band 3 membrane domain in tubular crystals (2010)
  • Author(s): Yamaguchi, T., Fujii, T., Abe, T., Hirai, T., Kang, D., Namba, K., Hamasaki, N., Mitsuoka, K
  • Journal Title: J. Struct. Biol Volume: vol. 169 Issue: 3 Pages: 406-412
  • DOI: 10.1016/j.jsb.2009.12.009
  • Peer Reviewed
• [Journal Article] Helical image reconstruction of the outward-open human erythrocyte band 3 membrane domain in tubular crystals (2010)
  • Author(s): Yamaguchi, T., Fujii, T., Abe, Y., Hirai, T., Kang, D., Namba, K., Hamasaki, N., Mitsuoka, K.
  • Journal Title: J Struct Biol. 169 Pages: 406-412
  • Peer Reviewed
• [Journal Article] Dissection of the assembly pathway of the proteasome lid in Saccharomyces cere visiae. (2010)
  • Author(s): Fukunaga, K., et al.
  • Journal Title: Biochem.Biophys.Res.Commun. Volume: 396 Pages: 1048-1053
  • Peer Reviewed
• [Journal Article] Crystal structure of yeast Rpn14, a chaperone of the 19S regulatory particle of the proteasome. (2010)
  • Author(s): Kim, S., et al.
  • Journal Title: J.Biol.Chem. Volume: 285 Pages: 15159-15166
  • Peer Reviewed
• [Journal Article] Helical image reconstruction of the outward-open human erythrocyte band 3 membrane domain in tubular crystals (2010)
  • Author(s): Yamaguchi, T., et al.
  • Journal Title: J.Sturct. Biol. 169 Pages: 406-412
  • Peer Reviewed
• [Journal Article] Multiple proteasome-interacting proteins assist the assembly of the yeast 19S regulatory particle (2009)
  • Author(s): Saeki, Y., Toh-E, A., Kudo, T., Kawamura, H., Tanaka, K
  • Journal Title: Cell Volume: vol. 137 Issue: 5 Pages: 900-913
  • DOI: 10.1016/j.cell.2009.05.005
  • Peer Reviewed
• [Journal Article] Multiple proteasome-interacting proteins assist the assembly of the yeast 19S regulatory particle (2009)
  • Author(s): Saeki, Y., Toh-e, A., Kudo, T., Kawamura, H., Tanaka, K.
  • Journal Title: Cell 29 Pages: 900-913
  • Peer Reviewed
• [Journal Article] Lysine 63-linked polyubiquitin chain may serve as a targeting signal for the 26S proteasome (2009)
  • Author(s): Saeki, Y., Kudo, T., Sone, T., Kikuchi, Y., Yokosawa, H., Toh-e, A., Tanaka, K.
  • Journal Title: EMBO J. 18 Pages: 359-371
  • Peer Reviewed
• [Journal Article] Multiple proteasome-interacting proteins assist the assembly of the yeast 19S regulatory particle. (2009)
  • Author(s): Saeki, Y., et al.
  • Journal Title: Cell 137 Pages: 900-913
  • Peer Reviewed
• [Journal Article] Lysnie 63-linked polyubiquitin chain may serve as a targeting signal for the 26S proteasome (2009)
  • Author(s): Saeki, Y., et al
  • Journal Title: The EMBO JOURNAL 28 Pages: 359-371
  • Peer Reviewed
• [Journal Article] Evaluation of a 2k CCD camera with an epitaxially grown CsI scintillator for recroding envery-filtered electron cryo-micrographs (2008)
  • Author(s): Yasunaga, T., Wakabayashi, T.
  • Journal Title: J. Electron Microsc. Volume: vol. 57 Issue: 3 Pages: 101-112
  • DOI: 10.1093/jmicro/dfn006
  • Peer Reviewed
• [Journal Article] Evaluation of a 2k CCD camera with an epitaxially grown CsI scintillator for recording energy-filtered electron cryo-micrographs (2008)
  • Author(s): Yasunaga, T., Wakabayashi, T.
  • Journal Title: J.Electron Microsc. 57 Pages: 101-112
  • NAID: 10025604019
  • Peer Reviewed
• [Journal Article] Cryo-EM structure of the native GroEL-GroES complex from thermus thermophilus encapsulating substrate inside the cavity (2008)
  • Author(s): Kanno, R., et al
  • Journal Title: Structure 17 Pages: 287-293
  • Peer Reviewed
• [Journal Article] Evaluation of a 2k CCD camera with an epitaxially-grown CsI scintillator for recording energy-filtered electron cryo-micrographs (2008)
  • Author(s): Yasunaga, T., et al
  • Journal Title: Journal of Electron Microscopy 57 Pages: 101-112
  • NAID: 10025604019
  • Peer Reviewed
• [Journal Article] 第一編ナノイメージングを可能にする顕微鏡法・第3章電子顕微鏡を用いたナノイメージング (2008)
  • Author(s): 安永卓生(共著)
  • Journal Title: ナノイメージング((株)NTS) Pages: 15-15
• [Presentation] Structural analysis of the 26S proteasome by cryo-electron micirscopy and single-particle analysis (2012)
  • Author(s): Wang, Z
  • Organizer: 第50回日本生物物理学会年会
  • Place of Presentation: 名古屋大学東山キャンパス(愛知県)
  • Year and Date: 2012-09-23
• [Presentation] 極低温電子顕微鏡による26Sプロテアソームの単粒子解析 (2012)
  • Author(s): 光岡薫
  • Organizer: 第50回日本生物物理学会年会
  • Place of Presentation: 名古屋大学東山キャンパス
• [Presentation] Structural analysis of the 26S proteasome by cryo-electron microscopy and Single-Particle Analysis (2012)
  • Author(s): Wang, Z.
  • Organizer: 第50回日本生物物理学会年会
  • Place of Presentation: 名古屋大学東山キャンパス
• [Presentation] 3D sturcture of the 26S proteasome by high-resolution electron cryo-microscopy (2011)
  • Author(s): Okuma, Y., et al
  • Organizer: 日本生物物理学会第49回年会
  • Place of Presentation: 兵庫県立大学・姫路書写キャンパス(兵庫県)
  • Year and Date: 2011-09-16
• [Presentation] 3D structure of the 26S proteasome by high-resolution electron cryo-microscopy (2011)
  • Author(s): Okuma, Y.
  • Organizer: 第49回日本生物物理学会年会
  • Place of Presentation: 兵庫県立大学姫路書写キャンパス(兵庫県)
  • Year and Date: 2011-09-11
• [Presentation] GraFixによる26Sプロテアソームの単粒子解析試料作製 (2011)
  • Author(s): 光岡薫, et al
  • Organizer: 日本顕微鏡学会第67回学術講演会
  • Place of Presentation: 福岡国際会議場(福岡県)
  • Year and Date: 2011-05-16
• [Presentation] Single Particle analysis of the 26S proteasome by high-resolution electron cryo-microscopy and advanced image analysis (2010)
  • Author(s): Okuma, Y
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 東北大学川内北キャンパス(福島県)
  • Year and Date: 2010-09-21
• [Presentation] Single particle analysis of the 26S proteasome by high-resolution electron cryo-microscopy and advanced image analysis (2010)
  • Author(s): Okuma, Y., Kasuya, D., Mitsuoka, K., Saeki, Y., Yasunaga, T.
  • Organizer: 日本生物物理学会第48回年会
  • Place of Presentation: 東北大学・川内北キャンパス
  • Year and Date: 2010-09-21
• [Presentation] クライオ電子顕微鏡法の現状と将来(招待講演) (2008)
  • Author(s): 安永卓生
  • Organizer: 顕微鏡学会・九州支部会
  • Place of Presentation: 久留米大学
  • Year and Date: 2008-12-06
• [Presentation] 「電子顕微鏡でみえるものはなにか」(招待講演) (2008)
  • Author(s): 安永卓生
  • Organizer: P&P先端技術講演会
  • Place of Presentation: 九州大学
  • Year and Date: 2008-10-16
• [Presentation] 電子顕微鏡画像処理システムEos2の構築 (2008)
  • Author(s): 安永卓生、岩崎憲治、宮澤淳夫
  • Organizer: 第46回日本生物物理学会
  • Place of Presentation: 福岡国際センター
• [Presentation] 多種の処理ツールの統合による半自動3次元再構成システムの開発 (2008)
  • Author(s): 新名人士、谷口香苗、西野有里、岩崎憲治、高木淳一、宮澤淳夫、光岡薫、安永卓生
  • Organizer: 第46回日本生物物理学会
  • Place of Presentation: 福岡国際センター
• [Presentation] 2+3次元画像を用いた、可視化・可聴化による特徴像の解析補助ツールの開発2 (2008)
  • Author(s): 瀧本雄介, 安永卓生
  • Organizer: 第46回日本生物物理学会
  • Place of Presentation: 福岡国際センター
• [Book] Biological Chemistry (2012)
  • Author(s): Tanaka, K. et al
  • Total Pages: 15
• [Book] Biological Chemistry (2012)
  • Author(s): Tanaka, K., et al
  • Total Pages: 17
  • Publisher: The proteasome : molecular machinery and pathological roles
• [Book] ナノイメージング(第一編ナノイメージングを可能にする顕微鏡法・第3章電子顕微鏡を用いたナノイメージング) (2008)
  • Author(s): 安永卓生(共著)
  • Total Pages: 15
  • Publisher: (株)NTS
• [Remarks] ホームページ
  • URL: http://www.rinshoken.or.jp/activities/t090514.html
• [Remarks]
  • URL: http://www.rinshoken.or.jp/activities/t090514.html