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Live imaging analysis of axis formation in mouse embryo

Research Project

Project/Area Number 20370088
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Developmental biology
Research InstitutionNational Institute for Basic Biology

Principal Investigator

FUJIMORI Toshihiko  National Institute for Basic Biology, 初期発生研究部門, 教授 (80301274)

Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥20,800,000 (Direct Cost: ¥16,000,000、Indirect Cost: ¥4,800,000)
Fiscal Year 2010: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2009: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2008: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
Keywordsマウス / 軸形成 / イメージング / 形態形成
Research Abstract

The aim of this project was to understand early events during early mouse embryonic development, from fertilization to formation of initial axis, by the continuous imaging of developing animals. In addition to a transgenic mouse line to visualize nuclei by fluorescent proteins, lines to visualize cell membrane, and future anterior region during initial axis formation were established. Techniques to observe embryonic development throughout early stages from fertilization to axis formation have been developed. With this combination, cell behavior including cell division and migration were analyzed.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (63 results)

All 2011 2010 2009 2008 Other

All Journal Article (18 results) (of which Peer Reviewed: 17 results) Presentation (33 results) Book (4 results) Remarks (8 results)

  • [Journal Article] Analysis of ciliary beat frequency and ovum transport ability in the mouse oviduct.2011

    • Author(s)
      Shi D., Komatsu K., Uemura T., Fujimori T.
    • Journal Title

      Genes to Cells 16

      Pages: 282-90

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Roles of PDGF receptor-beta in the structure and function of postnatal kidney glomerulus.2011

    • Author(s)
      Nakagawa T., Izumino K., Ishii Y., Oya T., Hamashima T., Jie S., Tomoda F., Fujimori T., Nabeshima Y., Inoue H., Sasahara M.
    • Journal Title

      Nephrol Dial Transplant. 26

      Pages: 458-68

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Roles of PDGF receptor-beta in the structure and function of postnatal kidney glomerulus.2011

    • Author(s)
      Nakagawa, T.
    • Journal Title

      Nephrol Dial Transplant.

      Volume: 26 Pages: 458-68

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of ciliary beat frequency and ovum transport ability in the mouse oviduct.2011

    • Author(s)
      Shi, D.
    • Journal Title

      Genes to Cells

      Volume: 16 Pages: 282-90

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ライブセルイメージングによる着床前胚発生研究2011

    • Author(s)
      小松紘司
    • Journal Title

      HORMONE FRONTIER IN GYNECOLOGY

      Volume: 18 Pages: 13-18

    • Related Report
      2010 Annual Research Report
  • [Journal Article] Preimplantation development of mouse : A view from cellular behavior.2010

    • Author(s)
      Toshihiko Fujimori
    • Journal Title

      Development, Growth & Differentiation 52

      Pages: 253-262

    • NAID

      10027564020

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Stem cell systems in development of mammals.2010

    • Author(s)
      Hitoshi Niwa, Toshihiko Fujimori
    • Journal Title

      Development, Growth & Differentiation 52

      Pages: 251-251

    • NAID

      10027564019

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Neuroprotective effects of PDGF against oxidative stress and the signaling pathway involved.2010

    • Author(s)
      Zheng L., Ishii Y., Tokunaga A., Hamashima T., Shen J., Zhao QL., Ishizawa S., Fujimroi T., Nabeshima Y., Mori H., Kondo T., Sasahara M.
    • Journal Title

      J Neurosci.Res. 88

      Pages: 1273-84

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Lack of cadherins Celsr2 and Celsr3 impairs ependymal ciliogenesis, leading to fata hydrocephalus.2010

    • Author(s)
      Fadel Tissir, Yibo Qu, Mireille Montcouquiol, Libing Zhou, Kouji Komatsu, Dongbo Shi, Toshihiko Fujimori, Jason Labeau, Donatienne Tyteca, Pierre Courtoy, Yves Poumay, Tadashi Uemura, Andre M Goffinet
    • Journal Title

      Nature Neuroscience 13

      Pages: 700-707

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Lack of cadherins Celsr2 and Celsr3 impairs ependymal ciliogenesis, leading to fatal hydrocephalus.2010

    • Author(s)
      Fadel Tissir
    • Journal Title

      Nature Neuroscience

      Volume: 13 Pages: 700-7

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Preimplantation development of mouse : A view from cellular behavior.2010

    • Author(s)
      Toshihiko Fujimori
    • Journal Title

      Development, Growth & Differentiation

      Volume: 52 Pages: 253-62

    • NAID

      10027564020

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Stem cell systems in development of mammals.2010

    • Author(s)
      Hitoshi Niwa
    • Journal Title

      Development, Growth & Differentiation

      Volume: 52 Pages: 251-251

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Neuroprotective effects of PDGF against oxidative stress and the signaling pathway involved.2010

    • Author(s)
      Zheng, L.
    • Journal Title

      J Neurosci.Res.

      Volume: 88 Pages: 1273-84

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Preimplantation development of mouse : A view from cellular behavior2010

    • Author(s)
      Toshihiko Fujimori
    • Journal Title

      Dev.Growth Differ. 52

      Pages: 253-262

    • NAID

      10027564020

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Morphological organization of the mouse preimplantation embryo.2009

    • Author(s)
      Fujimori T., Kurotaki Y., Komatsu K.,Nabeshima Y.
    • Journal Title

      Reprod Sci. 16

      Pages: 171-7

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of neuroprogenitor cells expressing the PDGF beta-receptor within the subventricular zone of postnatal mice.2008

    • Author(s)
      Ishi i Y., Matsumoto Y., Watanabe R., Elmi M., Fujimori T., Nissen J., Cao Y., Nabeshima Y., Sasahara M., Funa K.
    • Journal Title

      Mol Cell Neurosci 37

      Pages: 507-18

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] PDGF receptor beta is a potent regulator of mesenchymal stromal cell function.2008

    • Author(s)
      Tokunaga A., Oya T., Ishii Y., Motomura H., Nakamura C., Ishizawa S., Fujimori T., Nabeshima Y., Umezawa A., Kanamori M., Kimura T., Sasahara M.
    • Journal Title

      J Bone Miner Res 23

      Pages: 1519-28

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Deficiency of Zonula Occludens-1 Causes Embryonic Lethal Phenotype Associated with Defected Yolk Sac Angiogenesis and Apoptosis of Embryonic Cells.2008

    • Author(s)
      Katsuno T., Umeda K., Matsui T., Hata M., Tamura A., Itoh M., Takeuchi K., Fujimori T., Nabeshima Y. I., Noda T., Tsukita S.
    • Journal Title

      Mol Biol Cell 19

      Pages: 2465-2475

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Presentation] 藤森俊彦 Single cell level analysis of the changes of Nanog gene expression in living preimplantation mouse embryos.2010

    • Author(s)
      藤森俊彦
    • Organizer
      第16回武田科学振興財団生命科学シンポジウム
    • Place of Presentation
      Tokyo(poster)
    • Related Report
      2010 Final Research Report
  • [Presentation] ほ乳類初期発生の理解に向けて求めるもの2010

    • Author(s)
      藤森俊彦
    • Organizer
      定量生物学の会第3回年会
    • Place of Presentation
      Tokyo(poster)
    • Related Report
      2010 Final Research Report
  • [Presentation] マウス卵管上皮における膜タンパク質の局在の極性定量化2010

    • Author(s)
      石東博、小松紘司、平尾真由美、上村匡、藤森俊彦
    • Organizer
      定量生物学の会第3回年会
    • Place of Presentation
      Tokyo(poster)
    • Related Report
      2010 Final Research Report
  • [Presentation] Cellular behaviors in early mammalian embryonic development2010

    • Author(s)
      藤森俊彦
    • Organizer
      第48回日本生物物理学会年会
    • Place of Presentation
      Sendai
    • Related Report
      2010 Final Research Report
  • [Presentation] マウス初期胚における細胞と遺伝子の挙動の解析2010

    • Author(s)
      藤森俊彦
    • Organizer
      第51回日本組織細胞化学会総会・学術集会
    • Place of Presentation
      Tokyo(invited)
    • Related Report
      2010 Final Research Report
  • [Presentation] Analysis of Nanog expression during blastocyst formation in mouse2010

    • Author(s)
      藤森俊彦, 藤森俊彦
    • Organizer
      第43回日本発生生物学会年会
    • Place of Presentation
      Kyoto(poster)
    • Related Report
      2010 Final Research Report
  • [Presentation] Analysis of ciliary beat frequency and ovum transport ability in mouse oviduct2010

    • Author(s)
      Dongbo Shi, Koji Komatsu, Tadashi Uemura, Toshihiko Fujimori
    • Organizer
      第43回日本発生生物学会年会
    • Place of Presentation
      Kyoto(poster)
    • Related Report
      2010 Final Research Report
  • [Presentation] 新たに広がる哺乳類初期発生研究の可能性2010

    • Author(s)
      藤森俊彦
    • Organizer
      第62回日本細胞生物学会大会
    • Place of Presentation
      Osaka
    • Related Report
      2010 Final Research Report
  • [Presentation] Behaviors of Cell and Gene Expression during the Preimplantation Mouse Embryo2010

    • Author(s)
      藤森俊彦
    • Organizer
      The First SKLRB Symposia on Frontiers in Periimplantation Biology
    • Place of Presentation
      Beijing, China(invited)
    • Related Report
      2010 Final Research Report
  • [Presentation] Analysis of ciliary beat frequency and ovum transport activity in mouse oviduct.2010

    • Author(s)
      Dongbo Shi, Koji Komatsu, Tadashi Uemura, Toshihiko Fujimori
    • Organizer
      The First SKLRB Symposia on Frontiers in Periimplantation Biology
    • Place of Presentation
      Beijing, China(poster)
    • Related Report
      2010 Final Research Report
  • [Presentation] マウス初期発生研究における定量化2010

    • Author(s)
      藤森俊彦
    • Organizer
      定量生物学の会 第2回年会
    • Place of Presentation
      Osaka(poster)
    • Related Report
      2010 Final Research Report
  • [Presentation] Behaviors of Cell and Gene Expression during the Preimplantation Mouse Embryo2010

    • Author(s)
      Toshihiko Fujimori
    • Organizer
      The First SKLRB Symposia on Frontiers in Periimplantation Biology
    • Place of Presentation
      中国 北京市・北京友好ホテル
    • Related Report
      2010 Annual Research Report
  • [Presentation] Analysis of ciliary beat frequency and ovum transport activity in mouse oviduct2010

    • Author(s)
      Dongbo Shi
    • Organizer
      The First SKLRB Symposia on Frontiers in Periimplantation Biology
    • Place of Presentation
      中国 北京市・北京友好ホテル
    • Related Report
      2010 Annual Research Report
  • [Presentation] 新たに広がる哺乳類初期発生研究の可能性2010

    • Author(s)
      藤森俊彦
    • Organizer
      第62回日本細胞生物学会大会
    • Place of Presentation
      大阪市・大阪国際会議場
    • Related Report
      2010 Annual Research Report
  • [Presentation] Analysis of ciliary beat frequency and ovum transport ability in mouse oviduct2010

    • Author(s)
      Dongbo Shi
    • Organizer
      第43回日本発生生物学会年会
    • Place of Presentation
      京都市・京都国際会館
    • Related Report
      2010 Annual Research Report
  • [Presentation] Analysis of Nanog expression during blastocyst formation in mouse2010

    • Author(s)
      Kouji Komatsu
    • Organizer
      第43回日本発生生物学会年会
    • Place of Presentation
      京都市・京都国際会館
    • Related Report
      2010 Annual Research Report
  • [Presentation] マウス初期胚における細胞と遺伝子の挙動の解析2010

    • Author(s)
      藤森俊彦
    • Organizer
      第51回日本組織細胞化学会総会・学術集会
    • Place of Presentation
      東京都・秋葉原コンベンションホール
    • Related Report
      2010 Annual Research Report
  • [Presentation] Cellular behaviors in early mammalian embryonic development2010

    • Author(s)
      Toshihiko Fujimori
    • Organizer
      第48回日本生物物理学会年会
    • Place of Presentation
      仙台市・東北大学
    • Related Report
      2010 Annual Research Report
  • [Presentation] ほ乳類初期発生の理解に向けて求めるもの2010

    • Author(s)
      藤森俊彦
    • Organizer
      定量生物学の会第3回年会
    • Place of Presentation
      東京都・東京大学生産技術研究所
    • Related Report
      2010 Annual Research Report
  • [Presentation] マウス卵管上皮における膜タンパク質の局在の極性定量化2010

    • Author(s)
      石東博
    • Organizer
      定量生物学の会第3回年会
    • Place of Presentation
      東京都・東京大学生産技術研究所
    • Related Report
      2010 Annual Research Report
  • [Presentation] Single cell level analysis of the changes of Nanog gene expression in living preimplantation mouse embryos2010

    • Author(s)
      Kouji Komatsu
    • Organizer
      第16回武田科学振興財団生命科学シンポジウム
    • Place of Presentation
      東京都・シェラトン都ホテル東京
    • Related Report
      2010 Annual Research Report
  • [Presentation] マウス初期発生における細胞の分裂と形態形成2009

    • Author(s)
      藤森俊彦
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸市・ポートピアホテル
    • Year and Date
      2009-10-21
    • Related Report
      2009 Annual Research Report
  • [Presentation] マウス初期胚のライブイメージング2009

    • Author(s)
      藤森俊彦
    • Organizer
      奈良先端科学技術大学院大学シンポジウム「視る生物学4」
    • Place of Presentation
      Nara(invited)
    • Related Report
      2010 Final Research Report
  • [Presentation] Cell divisions relating to the morphogenesis of the early mouse embryo2009

    • Author(s)
      藤森俊彦
    • Organizer
      マウス初期発生における細胞の分裂と形態形成、第82回日本生化学会大会
    • Place of Presentation
      Kobe(invited)
    • Related Report
      2010 Final Research Report
  • [Presentation] Cell behaviors and the differentiation of cells during preimplantation mouse development.2009

    • Author(s)
      藤森俊彦
    • Organizer
      42^<nd> Annual Meeting for the Japanese Society of Developmental Biologists
    • Place of Presentation
      Niigata, Japan(invited)
    • Related Report
      2010 Final Research Report
  • [Presentation] Analysis of Cell Behaviors in Early Mouse Development.2009

    • Author(s)
      藤森俊彦
    • Organizer
      第9回NIBB-EMBL Symposium
    • Place of Presentation
      Okazaki,Japan
    • Related Report
      2010 Final Research Report
  • [Presentation] Analysis of cell behaviors during preimplantation mouse development. Keystone Symposia2009

    • Author(s)
      藤森俊彦
    • Organizer
      Frontiers in Reproductive Biologyand Regulation of Fertility
    • Place of Presentation
      Santa Fe(Invited)
    • Related Report
      2010 Final Research Report
  • [Presentation] 胚の形作りと関連する局所的細胞周期2008

    • Author(s)
      藤森俊彦
    • Organizer
      日本分子生物学会第31回年会シンポジウム
    • Place of Presentation
      神戸市
    • Year and Date
      2008-12-11
    • Related Report
      2008 Annual Research Report
  • [Presentation] Analysis of cell behaviors in early mouse embryos.2008

    • Author(s)
      藤森俊彦
    • Organizer
      SanFrancisco-Japan Joint Meeting on Vertebrate Organogenesis
    • Place of Presentation
      San Francisco(invited)
    • Related Report
      2010 Final Research Report
  • [Presentation] Analysis of cell behaviors in early mouse embryos.2008

    • Author(s)
      藤森俊彦
    • Organizer
      Joint Symposium : Vertebrate Development and Organogenesis
    • Place of Presentation
      Houston(invited)
    • Related Report
      2010 Final Research Report
  • [Presentation] The analysis of cell movement and expression of Oct4, Nanog and Cdx2 during mouse blastocyst formation.2008

    • Author(s)
      Kouji Komatsu, Yo-ichi Nabeshima, Toshihiko Fujimori
    • Organizer
      Joint meeting of the French and Japanese Society for Developmental Biology
    • Place of Presentation
      Giens peninsula,France(poster)
    • Related Report
      2010 Final Research Report
  • [Presentation] The expression of Cdx2 changes according to the position of cells in mouse blastocyst formation.2008

    • Author(s)
      Kouji Komatsu, Yo-ichi Nabeshima, Toshihiko Fujimori
    • Organizer
      第41回日本発生生物学会大会
    • Place of Presentation
      徳島(ポスター発表)
    • Related Report
      2010 Final Research Report
  • [Presentation] 胚の形作りと関連する局所的細胞周期2008

    • Author(s)
      藤森俊彦
    • Organizer
      日本分子生物学会第31回年会シンポジウム「細胞周期制御と高次生命現象」
    • Place of Presentation
      神戸(招待講演)
    • Related Report
      2010 Final Research Report
  • [Book] HORMONE FRONTIER IN GYNECOLOGY 18(ライブセルイメージングによる着床前胚発生研究 No.1)2011

    • Author(s)
      小松紘司、藤森俊彦
    • Related Report
      2010 Final Research Report
  • [Book] マウス初期胚における細胞分裂と発生、細胞周期フロンティア2010

    • Author(s)
      藤森俊彦
    • Publisher
      共立出版
    • Related Report
      2010 Final Research Report
  • [Book] マウス初期胚における細胞分裂と発生 細胞周期フロンティア2010

    • Author(s)
      藤森俊彦
    • Total Pages
      252
    • Publisher
      共立出版
    • Related Report
      2010 Annual Research Report
  • [Book] 実験医学 26(マウス着床前胚のライブイメージング No.17)2008

    • Author(s)
      藤森俊彦
    • Related Report
      2010 Final Research Report
  • [Remarks] ホームページ等

    • Related Report
      2010 Final Research Report
  • [Remarks] アウトリーチ活動

    • Related Report
      2010 Final Research Report
  • [Remarks] (1)基礎生物学研究所一般公開、体作りの始まり~卵から体ができるまで~、Oct.2, 2010(Okazaki)

    • Related Report
      2010 Final Research Report
  • [Remarks] (2)藤森俊彦、哺乳類初期発生の理解の為のライブイメージング、第9回自然科学研究機構シンポジウム、ビックリ4Dで見るサイエンスの革新、Mar.21 2010(Tokyo)

    • Related Report
      2010 Final Research Report
  • [Remarks] (3)藤森俊彦、基礎生物学研究所・生物学オリンピック選手合宿講義(発生学)「脊椎動物の発生」、Dec.25,2009.

    • Related Report
      2010 Final Research Report
  • [Remarks] (4)藤森俊彦、愛知県立岡崎高等学校スーパーサイエンスハイスクール出張講義、「哺乳類の体の形作りの始まり」Dec.8,2009

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.nibb.ac.jp/sections/fujimori.html

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.nibb.ac.jp/sections/fujimori.html

    • Related Report
      2009 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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