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Regulation of biomass-degrading enzymes via interaction of transcription factors

Research Project

Project/Area Number 20380048
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied microbiology
Research InstitutionNagoya University

Principal Investigator

KOBAYASGI Tetsuo  Nagoya University, 生命農学研究科, 教授 (20170334)

Co-Investigator(Kenkyū-buntansha) KATO Masashi  名古屋大学, 大学院・生命農学研究科, 准教授 (70242849)
KANAMARU Kyoko  名古屋大学, 大学院・生命農学研究科, 助教 (00420365)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥14,820,000 (Direct Cost: ¥11,400,000、Indirect Cost: ¥3,420,000)
Fiscal Year 2010: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2009: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2008: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
Keywords菌類 / 発現制御 / バイオマス / 応用微生物 / 遺伝子 / 真菌類 / キシラナーゼ / セルラーゼ / マンナナーゼ / 糸状菌
Research Abstract

Researches on transcription factors involved in production of biomass-degrading enzymes led to 1) identification of ManR and CelR that regulate production of mannolytic and cellulolytic enzymes in A. oryzae and cellulolytic enzymes in A. nidulans, respectively, 2) identification of target genes of AraR and ManR, 3) discovery of D-xylose-dependent reversible phosphorylation of XlnR, 4) identification of genes cooperatively regulated and those competitively regulated by XlnR and AraR, and 5) elucidation of involvement of McmA in cellulase regulation.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (20 results)

All 2011 2010 2009 2008

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (15 results) Book (1 results)

  • [Journal Article] Xylose triggers reversible phosphorylation of XlnR, the fungal transcriptional activator of xylanolytic and cellulolytic genes in Aspergillus oryzae.2011

    • Author(s)
      Noguchi Y, Tanaka H, Kanamaru K, Kato M, Kobayashi T
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry 75

      Pages: 953-959

    • NAID

      10028272791

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Xylose triggers reversible phosphorylation of XInR, the fungal transcriptional activator of xylanolytic and cellulolytic genes in Aspergillus oryzae2011

    • Author(s)
      Noguchi Y, Tanaka H, Kanamaru K, Kato M, Kobayashi T
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 印刷中(未定,掲載確定)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genes regulated by AoXlnR, the xylanolytic and cellulolytic transcriptional regulator, in Aspergillus oryzae.2009

    • Author(s)
      Noguchi Y, Sano M, Kanamaru K, Ko T, Takeuchi M, Kato M, Kobayashi T
    • Journal Title

      Appl Microbiol Biotechnol 85

      Pages: 141-154

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Genes regulated by AoXlnR, the xylanolytic and cellulolytic transcriptional regulator, in Aspergillus oryzae2009

    • Author(s)
      Y.Noguchi, M.Sano, K.Kanamaru, T.Ko, M.Takeuchi, M.Kato, T.Kobayashi
    • Journal Title

      Applied Microbiology and Biotechnology 85

      Pages: 141-154

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 麹菌におけるペントース代謝系とその制御2011

    • Author(s)
      渥美元規、野口祐司、小川真弘、金丸京子、加藤雅士、小山泰二、小林哲夫
    • Organizer
      日本農芸化学会2011年度大会(震災のため講演中止となったが要旨集の出版をもって発表済みの取り扱い)
    • Place of Presentation
      京都
    • Related Report
      2010 Final Research Report
  • [Presentation] 麹菌転写因子XlnRの誘導物質依存的な修飾とその生理的意義2011

    • Author(s)
      石川周平、野口祐司、金丸京子、加藤雅士、小林哲夫
    • Organizer
      日本農芸化学会2011年度大会(震災のため講演中止となったが要旨集の出版をもって発表済みの取り扱い)
    • Place of Presentation
      京都
    • Related Report
      2010 Final Research Report
  • [Presentation] Aspergillus nidulansにおけるセルラーゼ遺伝子の転写制御2011

    • Author(s)
      山川陽平、遠藤良知、金丸京子、加藤雅士、小林哲夫
    • Organizer
      日本農芸化学会2011年度大会(震災のため講演中止となったが要旨集の出版をもって発表済みの取り扱い)
    • Place of Presentation
      京都
    • Related Report
      2010 Final Research Report
  • [Presentation] Pentose catabolism and its regulation in Aspergillus oryzae2010

    • Author(s)
      T.Kobyahashi, Y.Noguchi, M.Atsumi, M.Ogawa, K.Kanamaru, K.Koyama
    • Organizer
      9th International Mycological Congress
    • Place of Presentation
      Edinborough, uk
    • Year and Date
      2010-08-05
    • Related Report
      2010 Annual Research Report
  • [Presentation] Transcriptional regulation of genes involved in hemicellulose and cellulose utilization in Aspergillus oryzae2010

    • Author(s)
      T.Kobayashi
    • Organizer
      Special Interest Group Meeting at 9th International My cological Congress
    • Place of Presentation
      Edinborough, uk
    • Year and Date
      2010-08-01
    • Related Report
      2010 Annual Research Report
  • [Presentation] Aspergillus nidulansにおけるセルラーゼ遺伝子の発現制御機構2010

    • Author(s)
      山川陽平、遠藤良知、金丸京子、加藤雅士、小林哲夫
    • Organizer
      第10回糸状菌分子生物学コンファレンス
    • Place of Presentation
      広島
    • Related Report
      2010 Final Research Report
  • [Presentation] Transcriptional regulation of genes involved in hemicellulose and cellulose utilization in Aspergillus oryzae.2010

    • Author(s)
      小林哲夫
    • Organizer
      Special Interest Group Meeting at 9th International Mycological Congress
    • Place of Presentation
      Edinburgh, UK
    • Related Report
      2010 Final Research Report
  • [Presentation] Pentose catabolism and its regulation in Aspergillus oryzae2010

    • Author(s)
      小林哲夫, 野口祐二, 渥美元規, 小川真弘, 金丸京子, 小山泰二
    • Place of Presentation
      Edinburgh, UK
    • Related Report
      2010 Final Research Report
  • [Presentation] 麹菌マンナン加水分解酵素群転写制御因子はセルラーゼ遺伝子群も制御する2010

    • Author(s)
      小川真弘、高橋 理、町田雅之、小林哲夫、小山泰二
    • Organizer
      日本農芸化学会2010年度大会
    • Place of Presentation
      東京
    • Related Report
      2010 Final Research Report
  • [Presentation] 麹菌におけるペントース代謝2009

    • Author(s)
      渥美元規, 野口祐二, 金丸京子, 加藤雅士, 小林哲夫
    • Organizer
      第9回糸状菌分子生物学コンファレンス
    • Place of Presentation
      東京大学 (東京都)
    • Year and Date
      2009-11-18
    • Related Report
      2009 Annual Research Report
  • [Presentation] 麹菌におけるペントース代謝2009

    • Author(s)
      渥美元規、野口祐二、金丸京子、加藤雅士、小林哲夫
    • Organizer
      第9回糸状菌分子生物学コンファレンス
    • Place of Presentation
      東京
    • Related Report
      2010 Final Research Report
  • [Presentation] Post translational modification of AoXlnR, a key transcriptional regulator of biomass-degrading enzymes in Aspergillus oryzae2009

    • Author(s)
      野口祐二, 金丸京子, 加藤雅士, 小林哲夫
    • Organizer
      25th Fungal Genetics Conference at Asilomar
    • Place of Presentation
      Asilomar, USA
    • Related Report
      2010 Final Research Report
  • [Presentation] 麹菌マンナナーゼ転写制御因子manRのスクリーニングと機能解析2009

    • Author(s)
      小川真弘、戸田智美、徳岡昌文、高橋理、小林哲夫、町田雅之、小山泰二
    • Organizer
      日本農芸化学会2009年度大会
    • Place of Presentation
      福岡
    • Related Report
      2010 Final Research Report
  • [Presentation] DNAマイクロアレイによるAoXlnR2制御下遺伝子群の網羅的同定2008

    • Author(s)
      金田貴詳、小川真弘、野口祐司、金丸京子、加藤雅士、小山泰二、小林哲夫
    • Organizer
      第8回糸状菌分子生物学コンファレンス
    • Place of Presentation
      金沢
    • Related Report
      2010 Final Research Report
  • [Presentation] 糸状菌転写因子AoXlnRの翻訳後修飾2008

    • Author(s)
      野口祐二、金丸京子、加藤雅士、小林哲夫
    • Organizer
      第60回日本生物工学会大会
    • Place of Presentation
      仙台
    • Related Report
      2010 Final Research Report
  • [Book] Transcriptional Regulation in Aspergillus.(Aspergillus : Molecular Biology and Genomics.)(M. Machida and K. Gomi. Norfolk)2010

    • Author(s)
      Kobayashi T, Kato M
    • Publisher
      Caister Academic Press
    • Related Report
      2010 Final Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

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