Analysis of the functional network governing cell division and cell differentiation

• Project/Area Number: 20380055
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Tokyo University of Agriculture
• Principal Investigator: YOSHIKAWA Hirofumi Tokyo University of Agriculture, 応用生物科学部, 教授 (50175676)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000); Fiscal Year 2010: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2009: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2008: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
• Keywords: 遺伝子発現 / 細胞分裂 / 脂質合成 / 細胞分化 / クエン酸回路 / 機能ネットワーク / 微生物 / 遺伝子 / 発現制御 / 胞子形成

Research Abstract

Analysis of the physiological significance of the stationary phase cells will contribute to the further understanding of cellular functions and genetic control systems in the viewpoint of functional network. We found an essential gene for lipid synthesis interacted with cell divisional machinery and involved in septum formation. While, a gene in the TCA cycle was found to be essential only for sporulationand was suggested to be a critical factor proceeding to the cell differentiation by sensing energy level of the cell.

Research Products

• [Journal Article] Sequence-specific error profile of Illumina sequencers (2011)
  • Author(s): Nakamura K., Yoshikawa, H., Kanaya S., 他
  • Journal Title: Nucleic Acids Research
  • Peer Reviewed
• [Journal Article] Discovery of Genome-wide DNA Polymorphisms by Whole-genome Sequencing of an Ancestral Japonica Rice Cultivar (2011)
  • Author(s): Y.Arai-Kichise, Y.Shiwa, H.Nagasaki, H.Yoshikawa, K.Wakasa
  • Journal Title: Plant and Cell Physiology Volume: 52 Pages: 274-282
  • Peer Reviewed
• [Journal Article] Whole-genome resequencing shows numerous genes with nonsynonymous SNPs in the Japanese native cattle Kuchinoshima-Ushi (2011)
  • Author(s): R.Kawahara-Miki, Y.Shiwa, Y.Kichise, H.Yoshikawa, T.Kono
  • Journal Title: BMC Genomics Volume: 12
  • Peer Reviewed
• [Journal Article] Genome of Bacillus subtilis phage SP 10 : comparative analysis with phage SPO1. (2011)
  • Author(s): L.M.Yee, T.Matsumoto, K.Yano, K.Asai, H.Yoshikawa, 他
  • Journal Title: Biosci.Biotechnol.Biochem Volume: (in press)
  • NAID: 10028272756
  • Peer Reviewed
• [Journal Article] Comparative Studies of Genome-Wide Maps of Nucleosomes between Deletion Mutants of elp3 and hos2 Genes of Saccharomyces cerevisiae. (2011)
  • Author(s): T.Matsumoto, C-S.Yun, H.Yoshikawa, H.Nishida
  • Journal Title: PLoS ONE Volume: 6
  • Peer Reviewed
• [Journal Article] Regulation of nitrate assimilation in cyanobacteria (2011)
  • Author(s): Y.Ohashi, S.Watanabe, H.Yoshikawa, T.Omata, 他
  • Journal Title: J.Exp.Botany Volume: 62(4) Pages: 1411-1424
  • Peer Reviewed
• [Journal Article] 枯草菌の環境ストレス応答機構 (2010)
  • Author(s): 吉川博文
  • Journal Title: 日本乳酸菌学会誌 21巻、No.1(総説) Pages: 16-26
  • NAID: 10029752187
• [Journal Article] Novel antibacterial compounds specifically targeting the essential WalR response regulator. (2010)
  • Author(s): Yasuhiro G., Yoshikawa, H., Msadek T., Utsumi R.
  • Journal Title: J.Antibiotics 63 Pages: 127-134
  • NAID: 10030707938
  • Peer Reviewed
• [Journal Article] 枯草菌の環境ストレス応答機構 (2010)
  • Author(s): 吉川博文
  • Journal Title: Japanese Journal of Lactic Acid Bacteria Volume: 21 Pages: 16-26
  • NAID: 10029752187
• [Journal Article] Novel antibacterial compounds specifically targeting the essential WalR response regulator. (2010)
  • Author(s): Gotoh, Y., Furuta, E., Dubrac, S., YoshikaNa, H., Utsumi, R., 他
  • Journal Title: J.Antibiotics 63 Pages: 127-134
  • Peer Reviewed
• [Journal Article] Mesurements of transposition frequency of insertion sequence IS1 by GFP hop-on assay. (2010)
  • Author(s): Saito, T., Chibazakura, T., Tkahashi, K., Yoshikawa, H., Sekine, Y.
  • Journal Title: J.Gen.Appl.Microbiol. 56 Pages: 187-192
  • Peer Reviewed
• [Journal Article] Improvement of bacterial transformation efficiency using Plasmid Artificial Modification (PAM). (2009)
  • Author(s): Yasui K., Kano Y., Tanaka K., Watanabe K., Shimizu-Kadota M., Yoshikawa H., Suzuki T.
  • Journal Title: Nucleic Acids Res. 37(1)
  • Peer Reviewed
• [Journal Article] Improvement of bacterial transformation efficiencyusing Plasmid Artificial Modification (PAM). (2009)
  • Author(s): Yasu i, K., Kano, Y., Tanaka, k., Yoshikawa, H., Suzuki, T., 他
  • Journal Title: Nucleic Acids Res. 37
  • Peer Reviewed
• [Journal Article] NbIR Is a Novel One-Component Response Regulator in the Cyanobacterium Synechococcus elongatus PCC 7942. (2008)
  • Author(s): Hiroaki Kato, Taku Chibazakura, Hirofumi Yoshikawa.
  • Journal Title: Biosci. Biotechnol. Biochem. 72 Pages: 1072-1079
  • Peer Reviewed
• [Presentation] 枯草菌における脂質代謝と細胞分裂間の新規ネットワーク解析 (2011)
  • Author(s): 高田啓
  • Organizer: 日本農芸化学会2011年大会
  • Place of Presentation: 京都(発表は中止)
  • Year and Date: 2011-03-29
• [Presentation] 脂質代謝と細胞分裂を共役させるネットワークの解析 (2011)
  • Author(s): 高田啓
  • Organizer: 第5回日本ゲノム微生物学会年会
  • Place of Presentation: 仙台(発表は中止)
  • Year and Date: 2011-03-14
• [Presentation] 次世代シーケンサーのための微生物ゲノム変異解析パイプラインの構築 (2010)
  • Author(s): 志波優、兼崎友、松本貴嗣、矢嶋俊介、吉川博文
  • Organizer: 日本分子生物学会第33回年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-09
• [Presentation] Geobacillus kaustophilus HTA426の3種のイノシトール脱水素酵素オルソログの発現と機能の解析 (2010)
  • Author(s): 三本木あずさ、森永哲郎、志波優、古川博文、吉田健一, 他
  • Organizer: 日本分子生物学会第33回年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] Analysis of novel heat shock response mechanism mediated by the Initial nucleotide of transcription. (2010)
  • Author(s): Kobayashi, S.Ogawa, M., Matsumoto, M.Yoshikawa, H., 他
  • Organizer: The 11^<th> Asian and Oceanian Conference on Transcription.
  • Place of Presentation: Nakijin, Okinawa
  • Year and Date: 2010-07-03
• [Presentation] Fundamental heat shock response is generated by intrinsic mechanism of transcription initiation. (2010)
  • Author(s): 吉川博文
  • Organizer: The 11th Asian and Oceanian Conference on Transcription.
  • Place of Presentation: Nakijin,(Okinawa)
  • Year and Date: 2010-07-02
• [Presentation] Fundamental heat shock response is generated by intrinsic mechanism of transcription intiation (2010)
  • Author(s): Kobayashi, S., Ogawa, M., Chibazakura, T.Yoshikawa, H., 他
  • Organizer: The 11^<th> Asian and Oceanian Conference on Transcription.
  • Place of Presentation: Nakijin, Okinawa
  • Year and Date: 2010-07-02
• [Presentation] Genome resequencing and emerging issues in bacteria, rice and cattle. (2010)
  • Author(s): 吉川博文
  • Organizer: Illumina User symposium
  • Place of Presentation: プーケット(タイ)(招待講演)
  • Year and Date: 2010-04-13
• [Presentation] Genome resequencing and emerging issues in bacteria, rice and cattle. (2010)
  • Author(s): Yoshikawa, H., Shiwa, Y., 他
  • Organizer: Illumina User symposium
  • Place of Presentation: Phuket, Thailand(招待講演)
  • Year and Date: 2010-04-13
• [Presentation] Analysis workflows and experiences at NODAI genome research center (2010)
  • Author(s): Shiwa, Y.Yoshikawa, H., 他
  • Organizer: Illumina User symposium, Asia Pacific & Japan
  • Place of Presentation: Phuket, Thailand
  • Year and Date: 2010-04-13
• [Presentation] 枯草菌のRNAポリメラーゼに結合する小蛋白質(ω)の機能解析 (2010)
  • Author(s): 朝井計、松本貴嗣、佐久間萌里佳、矢野晃一、吉川博文
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-29
• [Presentation] 次世代シーケンサーによるSynechococcus elongatus PCC 7942のリシーケンス解析 (2010)
  • Author(s): 兼崎友、渡辺智、志波優、吉川博文
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-29
• [Presentation] Lactococcus lactis IO-1株におけるキシロース代謝関連遺伝子の転写解析 (2010)
  • Author(s): 鈴木成人、千葉櫻拓、園元謙二、門多真理子、吉川博文, 他
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-29
• [Presentation] 枯草菌ゲノムベクターシステムを利用したミニセルロソームの構築 (2010)
  • Author(s): 原裕一、中山薫、松岡聡、吉川博文
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-29
• [Presentation] 細胞分裂から胞子形成開始への移行に関与する新規ネットワークの探索 (2010)
  • Author(s): 横川朋美
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-28
• [Presentation] 枯草菌に見出されたdksAオルソログ機能未知遺伝子群の解析 (2010)
  • Author(s): 吉田麻衣、渡辺智、吉川博文
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-28
• [Presentation] シアノバクテリアSynechococcus elongatus PCC 7942株における複製開始点の同定 (2010)
  • Author(s): 渡辺智、兼崎友、大林龍胆、志波優、千葉櫻拓、吉川博文
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-28
• [Presentation] 曳糸性の安定化した納豆菌の育種 (2010)
  • Author(s): 加田茂樹、大河内悠貴、千葉櫻拓、関根靖彦、吉川博文, 他
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-28
• [Presentation] 次世代シーケンサーを用いたSynechococcus elongatus PCC 7942のリシーケンス解析 (2010)
  • Author(s): 兼崎友、渡辺智、志波優、吉川博文
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本
  • Year and Date: 2010-03-19
• [Presentation] シアノバクテリアSynechococcus elongatus PCC 7942における分子シャペロンDnaKとDnaJのパートナーシップ (2010)
  • Author(s): 葉山綾乃、柴本理宏、小澤慎吾、渡辺智、荷村かおり、吉川博文
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本
  • Year and Date: 2010-03-19
• [Presentation] シアノバクテリアSynechococcus elongatus PCC 7942株における複製開始点の同定 (2010)
  • Author(s): 渡辺智、兼崎友、大林龍胆、志波優、千葉櫻拓、吉川博文
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本
  • Year and Date: 2010-03-18
• [Presentation] 単細胞性紅藻Cyanidioschyzon merolaeにおけるGFPを用いた局在解析法 (2010)
  • Author(s): 佐藤淳、渡辺智、大沼みお、田中寛、吉川博文
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本
  • Year and Date: 2010-03-18
• [Presentation] バクテリアゲノムのリシーケンスによる変異解析とその問題点 (2010)
  • Author(s): 吉川博文
  • Organizer: 第4回日本ゲノム微生物学会
  • Place of Presentation: 福岡
  • Year and Date: 2010-03-08
• [Presentation] シアノバクテリアSynechococcus elongatus PCC 7942における分子シャペロンDnaKとDnaJのパートナーシップ (2010)
  • Author(s): 葉山綾乃、渡辺智、荷村(松根)かおり、仲本準、吉川博文, 他
  • Organizer: 第4回日本ゲノム微生物学会
  • Place of Presentation: 福岡
  • Year and Date: 2010-03-08
• [Presentation] Characterization of xylose metabolism in pentose phosphate pathway by Lactococcus lactis IO-1. (2009)
  • Author(s): 棚町香織、花田克浩、中東憲治、吉川博文、園元謙二, 他
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-12
• [Presentation] 次世代シーケンサーによるラン藻Synechococcus elongatus PCC 7942のリシーケンス (2009)
  • Author(s): 兼崎友、渡辺智、志波優、松本貴嗣、吉川博文, 他
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-11
• [Presentation] 酒米品種「雄町」のゲノム解読とSNPの検出 (2009)
  • Author(s): 吉瀬(新井)祐子、志波優、吉川博文、矢野昌裕、若狭暁
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-11
• [Presentation] 日本在来野生牛(口之島牛)の全ゲノム配列解析 (2009)
  • Author(s): 津田薫、川原玲香、志波優、矢嶋俊介、吉川博文、河野友宏, 他
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-11
• [Presentation] 枯草菌の環境ストレス応答機構 (2009)
  • Author(s): 吉川博文
  • Organizer: 日本乳酸菌学会(招待講演)
  • Place of Presentation: 東京
  • Year and Date: 2009-11-27
• [Presentation] Functional analysis of the stress response mechanism in cyanobacteria----a new aspect of molecular chaperones---- (2009)
  • Author(s): S.Watanabe, M.Sato, H.Yoshikawa
  • Organizer: Fourth International Congress on Stress Responses in Biology and Medicine(招待講演)
  • Place of Presentation: 札幌
  • Year and Date: 2009-10-08
• [Presentation] 乳酸菌Lactococcus lactis IO-1におけるキシロース代謝の機構解明 (2009)
  • Author(s): 棚町香織、中東憲治、門多真理子、園元謙二、吉川博文, 他
  • Organizer: 日本生物工学会2009年大会
  • Place of Presentation: 宮崎
  • Year and Date: 2009-09-24
• [Presentation] Involvement of G3P acyltransferase, PlsX, in the cell division machinery. (2009)
  • Author(s): Takada H.
  • Organizer: ASM Conference on Prokaryotic development.
  • Place of Presentation: Cambridge, MA. USA
  • Year and Date: 2009-07-02
• [Presentation] Involvement of G3P acyltransferase, PlsX, in the cell division machinery (2009)
  • Author(s): Takada, H., Yoshikawa H.
  • Organizer: ASM Conference on Prokaryotic development
  • Place of Presentation: Cambridge, MA.USA
  • Year and Date: 2009-07-02
• [Presentation] Involvement of G3P acyltransferase, PlsX, in the cell division machinery. (2009)
  • Author(s): Yoshikawa H.
  • Organizer: 5th International Conference on gram-positive Microorganisms.
  • Place of Presentation: San Diego, CA. USA
  • Year and Date: 2009-06-16
• [Presentation] Involvement of G3P acyltransferase, PlsX, in the cell division machinery (2009)
  • Author(s): Takada, H., Fukushima, S., Matsumoto, K., Yoshikawa H., 他
  • Organizer: 5th International Conference on gram-positive Microorganisms
  • Place of Presentation: San Diego, CA.USA
  • Year and Date: 2009-06-16
• [Presentation] 細胞分裂装置に関わる機能ネットワーク (2009)
  • Author(s): 吉川博文
  • Organizer: 日本農芸化学会シンポジウム
  • Place of Presentation: 福岡
  • Year and Date: 2009-03-29