Studies on diterpene-producing system based on the useful characters of unusual fungal chimera diterpene synthases.

• Project/Area Number: 20380064
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bioproduction chemistry/Bioorganic chemistry
• Research Institution: Yamagata University
• Principal Investigator: TOYOMASU Tomonobu Yamagata University, 農学部, 准教授 (60272085)
• Co-Investigator(Kenkyū-buntansha): SHIONO Yoshihito 山形大学, 農学部, 准教授 (80361278)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000); Fiscal Year 2010: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2009: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2008: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
• Keywords: 生合成 / ジテルペン / 高機能酵素 / 菌類 / 物質生産

Research Abstract

Unusual fungal diterpene synthases possess the GGDP synthase domain at C-terminus and GGDP cyclase domain at N-terminus. We planed to construct the diterpene producing system based on the useful characters of fungal chimera diterpene synthase family. Our research has provided interesting information by characterization of chimera enzymes from Aspergillus oryzae, Magnaporthe grisea, and Penicillium chrysogenum.

Research Products

• [Presentation] 糸状菌のキメラジテルペン合成酵素の植物体内発現 (2010)
  • Author(s): 千葉康隆, ら
  • Organizer: 植物化学調節学会第45回大会
  • Place of Presentation: 神戸大学(神戸)
  • Year and Date: 2010-11-02
• [Presentation] Functional analysis of chimera diterpene synthase in Aspergillus oryzae (2010)
  • Author(s): Chiba Y., et al.
  • Organizer: 20th International Conference on Plant Growth Substances
  • Place of Presentation: Spain
  • Year and Date: 2010-06-29
• [Presentation] Functional analysis of chimera diterpene synthase in Aspergillus oryzae (2010)
  • Author(s): Chiba, et al.
  • Organizer: 20^<th> International Conference on Plant Growth Substances
  • Place of Presentation: スペインタラゴナ
  • Year and Date: 2010-06-29
• [Presentation] 麹菌のジテルペン合成酵素について (2010)
  • Author(s): 千葉康隆, ら
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京大学(東京)
  • Year and Date: 2010-03-29
• [Presentation] イモチ病菌のジテルペン合成酵素について (2010)
  • Author(s): 後藤麻予, ら
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京大学(東京)
  • Year and Date: 2010-03-29
• [Presentation] 麹菌のジテルペン合成酵素について (2010)
  • Author(s): 千葉康隆, ら
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京大学(東京都目黒区)
  • Year and Date: 2010-03-29
• [Presentation] イモチ病菌のジテルペン合成酵素について (2010)
  • Author(s): 後藤麻予, ら
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京大学(東京都目黒区)
  • Year and Date: 2010-03-29
• [Presentation] Characterization of diterpene synthase genes in Aspergillus oryzae (2009)
  • Author(s): Chiba Y., et al.
  • Organizer: TERPNET 2009
  • Place of Presentation: 東京大学(東京)
  • Year and Date: 2009-05-26
• [Presentation] Characterization of diterpene synthase genes in Aspergillus oryzae. (2009)
  • Author(s): 千葉康隆, et al.
  • Organizer: TERPNET2009
  • Place of Presentation: 東京大学(東京都文京区)
  • Year and Date: 2009-05-26
• [Presentation] いもち病菌のジテルペン合成酵素に関する研究
  • Author(s): 後藤麻予, ら
  • Organizer: 日本農芸化学会2011年度大会(震災の影響で登録のみ(学会は成立))
• [Presentation] 青カビの分生子誘導物質の生合成遺伝子に関する研究
  • Author(s): 藤崎由記子, ら
  • Organizer: 日本農芸化学会2011年度大会(震災の影響で登録のみ(学会は成立))
• [Presentation] いもち病菌のジテルペン合成酵素に関する研究
  • Author(s): 後藤麻予, ら
  • Organizer: 日本農芸化学会2011年度大会
  • Place of Presentation: (震災の影響で登録のみ)
• [Presentation] 青カビの分生子誘導物質の生合成遺伝子に関する研究
  • Author(s): 藤崎由記子, ら
  • Organizer: 日本農芸化学会2011年度大会
  • Place of Presentation: (震災の影響で登録のみ)