| Project/Area Number |
20380089
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Forest science
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
NAKAGAWA Michiko 名古屋大学, 大学院・生命農学研究科, 准教授 (70447837)
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| Co-Investigator(Renkei-kenkyūsha) |
TSUMURA Yoshihiko 独立行政法人森林総合研究所, 森林遺伝研究領域, 領域長 (20353774)
UENO Saneyoshi 独立行政法人森林総合研究所, 森林遺伝研究領域, 主任研究員 (40414479)
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| Research Collaborator |
SUZUKI Setsuko 独立行政法人森林総合研究所, 森林遺伝研究領域, 研究員 (70456622)
ABE Kosei 名古屋大学, 大学院・生命農学研究科, 院生
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2010: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2009: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2008: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
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| Keywords | ブナ / EST / 核遺伝子 / 塩基配列変異 / 中立的変異 / 適応的変異 / 集団遺伝構造 / 遺伝的多様性 / 適応 / 塩基配列 / 中立変異 / 塩基多様度 / TajimaのD / マイクロサテライト / 一塩基多型 / SSR / 自然淘汰 / SNP |
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| Research Abstract |
In this study, we aimed to search candidate genes for adaptation by examining the variation at nuclear expressed regions in Fagus crenata and address ecological significance of the variation. We developed a total of one hundreds and sixty-nine genetic markers using the library of expressed sequence tags. We evaluated nucleotide variation at the targeted expressed regions in the populations on the Sea of Japan and Pacific sides of Japan, by sequencing with the designed primers for the genetic markers. Although no loci indicating adaptive variation were detected, the nucleotide variation suggests historical population expansion. The population genetic structure we found confirmed that F. crenata populations should be managed separately between the Sea of Japan and Pacific sides of Japan.
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