Development of a novel therapy for canine atopic dermatitis with chimera IgG of anti-canine IgE antibody

• Project/Area Number: 20380172
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Clinical veterinary science
• Research Institution: Gifu University
• Principal Investigator: MAEDA Sadatoshi Gifu University, 応用生物科学部, 教授 (50377694)
• Co-Investigator(Kenkyū-buntansha): MIZUNO Takuya 山口大学, 農学部, 准教授 (90398826)
• Research Collaborator: TSOHIHIRO Tsukui 日本全薬工業株式会社, 主任研究員
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000); Fiscal Year 2010: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2009: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2008: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
• Keywords: アトピー性皮膚炎 / 動物モデル / イヌ / IgE / アトピー性皮膚

Research Abstract

We tried to produce anti-canine IgE antibodies that could inhibit a binding of IgE to mast cells in order to develop a novel therapy for canine atopic dermatitis. Ten clones of the antibodies were generated by immunization of recombinant protein of the mast cell binding sites of IgE. The present results indicated that the antibodies could not inhibit a binding IgE to mast cells and also an allergen-mediated degranulation.

Research Products

• [Journal Article] Identification of the signaling pathway of TNF-alpha-induced CCL17/TARC transcription in a canine keratinocyte cell line (2011)
  • Author(s): Sanae Shibata、Sadatoshi Maeda、Naho Kondo、Naoki Chimura、Akiko Inoue、Tsuneo Fukata
  • Journal Title: Vet.Immunol.Immunopathol. 139 Pages: 90-98
  • Peer Reviewed
• [Journal Article] Effect of recombinant canine interferon-gamma on granulocyte-macrophage colony-stimulating factor、transforming growth factor-beta and CC chemokine ligand 17 mRNA transcription in a canine keratinocyte cell line (CPEK) (2011)
  • Author(s): Sanae Shibata、Sadatoshi Maeda、Naho Kondo、Akiko Tnoue、Naoki Chimura、Tsuneo Fukata
  • Journal Title: Vet.Dermatol. 22 Pages: 24-30
  • Peer Reviewed
• [Journal Article] Identification of the signaling pathway of TNF-alpha-induced CCL17/TARC transcription in a canine keratinocyte cell line (2011)
  • Author(s): Sadatoshi MAEDA (Sanae SHIBATA)
  • Journal Title: Veterinary Immunology and Immunopathology Volume: 139 Pages: 90-98
  • Peer Reviewed
• [Journal Article] Augmentation of CCL17 and CCL28 gene expression by TNF-alpha、IL-lbeta、or IFN-gamma in cultured canine keratinocytes (2010)
  • Author(s): Sanae Shibata、Sadatoshi Maeda、Naoki Chimura、Naho Kondo、Tsuneo Fukata
  • Journal Title: Res.Vet.Sci. 88 Pages: 422-426
  • Peer Reviewed
• [Journal Article] Augmentation of CCL17 and CCL28 gene expression by TNF-alpha, IL-1beta, or IFN-gamma in cultured canine keratinocytes (2010)
  • Author(s): Sadatoshi MAEDA (Sanae SHIBATA)
  • Journal Title: Research in Veterinary Science (In press)
  • Peer Reviewed
• [Journal Article] Effect of recombinant canine IFN-gamma on GM-CSF, TGF-beta and CCL17 mRNA transcription in canine keratinocytes cell line (CPEK) (2010)
  • Author(s): Sadatoshi MAEDA (Sanae Shibata)
  • Journal Title: Veterinary Dermatology (In press)
  • Peer Reviewed
• [Journal Article] House dust mite major allergen Der f 1 enhances proinflammatory cytokine and chemokine gene expression in a cell line of canine epidermal keratinocytes (2009)
  • Author(s): Shingo Maeda、Sadatoshi Maeda、Sanae Shibata、Naoki Chimura、Tsuneo Fukata
  • Journal Title: Vet.Immunol.Immunopathol. 131 Pages: 298-302
  • Peer Reviewed
• [Journal Article] Molecular cloning of canine protease-activated receptor-2 and its expression in normal dog tissues and atopic skin lesions (2009)
  • Author(s): Shingo Maeda、Sadatoshi Maeda、Sanae Shibata、Naoki Chimura、Tsuneo Fukata
  • Journal Title: J.Vet.Med.Sci. 71 Pages: 577-582
  • NAID: 130000134367
  • Peer Reviewed
• [Journal Article] Molecular cloning of canine interleukin-31 and its expression in various tissues (2009)
  • Author(s): Takuya Mizuno、Satoshi Kanbayashi、Takumi Okawa、Sadatoshi Maeda、Masaru Okuda
  • Journal Title: Vet Immunol Immunopathol. 131 Pages: 140-143
  • Peer Reviewed
• [Journal Article] Der f 1 enhances proinflammatory cytokine and chemokine gene expression in a cell line of canine epidermal keratinocytes (2009)
  • Author(s): Sadatoshi MAEDA (Shingo MAEDA)
  • Journal Title: Veterinary Immunology and Immunopathology 131 Pages: 298-302
  • Peer Reviewed
• [Journal Article] Molecular cloning of canine protease-activated receptor-2 and its expression in normal dog tissues and atopic skin lesion (2009)
  • Author(s): Sadatoshi MAEDA (Shingo MAEDA)
  • Journal Title: Journal of Veterinary Medical Science 71(In press)
  • NAID: 130000134367
  • Peer Reviewed
• [Journal Article] Phenotypic analysis for a cell line of canine epidermal keratinocytes (2008)
  • Author(s): Sanae Shibata、Sadatoshi Maeda、Hiromi Tsuchida、Tsuneo Fukata
  • Journal Title: J.Vet.Med.Scl. 70 Pages: 853-855
  • NAID: 110006878938
  • Peer Reviewed
• [Journal Article] Phenotypic analysis for a cell line of canine epidermal keratinocytes (2008)
  • Author(s): Sadatoshi MAEDA (Sanae SHIBATA)
  • Journal Title: Journal of Veterinary Medical Science 70 Pages: 853-855
  • NAID: 110006878938
  • Peer Reviewed
• [Journal Article] Suitable reference genes for quantitative real-time RT-PCR in total RNA extracted from canine whole blood using the PAXgene^<TM> system
  • Author(s): Naoki Chimura、Sanae Shibata、Tsuyoshi Kimura、Naho Kondo、Takashi Mori、Yuki Hoshino、Hiroaki Kamishina、Sadatoshi Maeda
  • Journal Title: J.Vet.Med.Sci. in press
  • NAID: 130000677100
  • Peer Reviewed
• [Presentation] イヌの上皮向性リンパ腫病変部におけるリンパ球遊走関連分子の遺伝子発現解析 (2010)
  • Author(s): 千村直輝(前田貞俊)
  • Organizer: 第150回日本獣医学会学術集会
  • Place of Presentation: 帯広
  • Year and Date: 2010-09-16
• [Presentation] Identification of signaling pathway for regulation of CCL17/TARC mRNA transcription in canine keratinocytes (2009)
  • Author(s): 柴田早苗(前田貞俊)
  • Organizer: 第6回アジア獣医皮膚科学会
  • Place of Presentation: 台北(台湾)
  • Year and Date: 2009-12-12
• [Presentation] アトピー性皮膚炎の免疫病態におけるケラチノサイトの役割 (2009)
  • Author(s): 前田貞俊(前田貞俊)
  • Organizer: 第6回動物サイトカイン研究会学術集会
  • Place of Presentation: 宮崎
  • Year and Date: 2009-09-26
• [Presentation] アトピー性皮膚炎の免疫病態におけるケラチノサイトの役割 (2008)
  • Author(s): 前田貞俊
  • Organizer: 第6回動物サイトカイン研究会学術集会
  • Place of Presentation: 宮崎大学
  • Year and Date: 2008-09-26