Molecular mechanism of the collapse of cell polarity and arrest of cell growth by metabolic stress
| Project/Area Number |
20380187
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Kyoto University |
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Principal Investigator |
INOUE Yoshiharu Kyoto University, 大学院・農学研究科, 准教授 (70203263)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000)
Fiscal Year 2010: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2009: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2008: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
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| Keywords | 酵母 / 代謝 / ストレス / 細胞分裂 / 極性成長 / ホスファチジルイノシトール / ボスファチジルイノシトール |
|---|
| Research Abstract |
Methylglyoxal (MG) is a natural metabolite derived from glycolysis. We found that the treatment of Saccharomyces cerevisiae cells with MG increases the intracellular levels of phosphatidylinositol (3, 5) bisphosphate. We also found that MG depolarizes actin cytoskeleton, thereby inhibiting the nuclear division of S. cerevisiae cells. The MG-induced depolarization of actin cytoskeleton was repressed by the expression of a constitutively active allele of PKC1 (Pkc1-R398P), and consequently, the nuclear division was partially restored. We also found that MG induces the phosphorylation of Tyr19 of Cdc28 in a Swe1-dependent manner. The disruption of SWE1 partially alleviated the inhibitory effect of MG on the nuclear division. The expression of Pkc1-R398P in swe1? cells caused the additive effect in terms of alleviation of the MG-induced inhibition of nuclear division.
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Report
(4 results)
Research Products
(20 results)
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[Journal Article] Calcineurin/Crzl destabilizes Msn2 and Msn4 in the nucleus in response to Ca^<2+> in2010
Author(s)
Takatsume, Y., Ohdate, T., Maeta, K., Nomura, W., Izawa, S., Inoue, Y.
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Journal Title
Saccharomyces cerevisiae. Biochemical J. 427(2)
Pages: 275-287
Related Report
Peer Reviewed
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