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Creation and Pharmaceutical Development of Intelligent Zinc Fingers Based on Genomic Chemistry

Research Project

Project/Area Number 20390037
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Drug development chemistry
Research InstitutionDoshisha Women's College of Liberal Arts

Principal Investigator

SUGIURA Yukio  Doshisha Women's College of Liberal Arts, 薬学部, 特任教授 (40025698)

Co-Investigator(Kenkyū-buntansha) NEGI Shigeru  同志社女子大学, 薬学部, 助教 (50378866)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000)
Fiscal Year 2010: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2009: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2008: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Keywords亜鉛フィンガー / 転写因子 / DNA結合 / タンパク質デザイン / マルチフィンガー / 人工転写因子 / 遺伝子制御 / Zif268 / マルチフインガー / GAGA因子
Research Abstract

By re-design of native zinc finger proteins, the following artificial proteins were created : (1) restriction enzyme-like molecule possessing DNA hydrolytic ability, (2) genome-regulating molecule with long DNA base recognition, and (3) metal-responsive artificial transcriptional switcher. The pharmaceutical application of these new proteins were investigated in this work.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (24 results)

All 2010 2009 2008

All Journal Article (13 results) (of which Peer Reviewed: 13 results) Presentation (9 results) Book (2 results)

  • [Journal Article] Zinc finger-zinc finger interaction between the transcription factors, GATA-1 and Spl, Biochem.Biophys.2010

    • Author(s)
      M.Imanishi, C.Imamura, C.Higashi, W.Yan, S.Negi, S.Futaki, Y.Sugiura
    • Journal Title

      Res.Commun. 400(4)

      Pages: 625-630

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Metal-stimulated transcriptional regulation by an aritificial zinc finger protein2010

    • Author(s)
      M.Imanishi, T.Nakaya, T.Morisaki, D.Noshiro, S.Futaki, Y.Sugiura
    • Journal Title

      ChemBioChem 11(12)

      Pages: 1653-1655

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] マルチ亜鉛フィンガー型人工転写因子の創製と機能2010

    • Author(s)
      森崎達也、今西未来、二木史朗、杉浦幸雄
    • Journal Title

      薬学雑誌 130(1)

      Pages: 45-48

    • NAID

      130000152009

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Metal-stimulated transcriptional regulation by an artificial zinc finger protein2010

    • Author(s)
      M, Imanishi
    • Journal Title

      ChemBioChem.

      Volume: 11 Pages: 1653-1655

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] マルチ亜鉛フィンガー型人工転写因子の創製と機能2010

    • Author(s)
      森崎達也
    • Journal Title

      薬学雑誌 130

      Pages: 45-48

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Novel zinc finger nuclease created by combining the Cys_2His_2 and His_4 type zinc finger domain2009

    • Author(s)
      S.Negi, Y.Umeda, S.Masuyama, Y.Sugiura
    • Journal Title

      Bioorg.Med.Chem.Lett. 19(10)

      Pages: 2789-2791

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Novel zinc finger nuclease Created by combining the Cys_2His_2 and His_4 type zinc finger domains2009

    • Author(s)
      S.Negi
    • Journal Title

      Bioorg.Med.Chem.Lett. 19

      Pages: 2789-2791

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of bulkiness and hydrophobicity of aliphatic amino acid at recognition helix of GAGA zinc finger on the stability of hydrophobic core and DNA-binding affinity2008

    • Author(s)
      M.Dhanasekaran, S.Negi, M.Imanishi, M.Suzuki, Y.Sugiura
    • Journal Title

      Biochemistry 47(45)

      Pages: 11717-11724

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Rapid transcriptional activity in vivo and slow DNA binding in vitro by artificial multi-zinc finger protein2008

    • Author(s)
      T.Morisaki, M.Imanishi, S.Futaki, Y.Sugiura
    • Journal Title

      Biochemistry 47(38)

      Pages: 10171-10177

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Novel redesigned zinc finger proteins : Design strategy and its application2008

    • Author(s)
      S.Negi, M.Imanishi, M.Matsumoto, Y.Sugiura
    • Journal Title

      Chem.Eur.J. 14(11)

      Pages: 3236-3249

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Novel redesigned zinc finger proteins : Design strategy and its application2008

    • Author(s)
      S. Negi
    • Journal Title

      Chem. Eur. J. 14

      Pages: 3236-3249

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rapid transcriptional activity in vivo and slow DNA binding invitro by artificial multi-zinc finger protein2008

    • Author(s)
      T. Morisaki
    • Journal Title

      Biochemistry 47

      Pages: 10171-10177

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of bulkiness and hydrophobicity of aliphatic amino acid at recognition helix of GAGA zinc finger on the stability of hydrophobic core and DNA-binding affinity2008

    • Author(s)
      M. Dhanasekaran
    • Journal Title

      Biochemistry 47

      Pages: 11717-11724

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] インテリジェント人工亜鉛フィンガー蛋白質の創製と医学・薬学領域への展開2010

    • Author(s)
      杉浦幸雄
    • Organizer
      日本薬学会東海支部特別講演会
    • Place of Presentation
      名古屋市
    • Year and Date
      2010-11-04
    • Related Report
      2010 Final Research Report
  • [Presentation] 亜鉛濃度に応答した亜鉛フィンガー転写因子の創製2010

    • Author(s)
      中屋智博、今西未来、森崎達也、能代大輔、二木史朗、杉浦幸雄
    • Organizer
      第20回金属が関与する生体関連反応シンポジウム
    • Place of Presentation
      徳島市
    • Year and Date
      2010-06-25
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] リデザインによる野生型亜鉛フィンガータンパク質の機能改変:人工亜鉛フィンガー型ヌクレアーゼの創生2010

    • Author(s)
      根木滋、三間紘子、杉浦幸雄
    • Organizer
      第10回日本蛋白質科学会年会
    • Place of Presentation
      札幌市
    • Year and Date
      2010-06-18
    • Related Report
      2010 Final Research Report
  • [Presentation] 生体と金属:生体に不可欠な元素2009

    • Author(s)
      杉浦幸雄
    • Organizer
      第14回国際生物無機化学会議(公開市民講座)
    • Place of Presentation
      名古屋市
    • Year and Date
      2009-07-25
    • Related Report
      2010 Final Research Report
  • [Presentation] 生体と金属-生体に不可欠な元素-2009

    • Author(s)
      杉浦幸雄
    • Organizer
      第14回国際生物無機化学会議公開市民講座
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2009-07-25
    • Related Report
      2009 Annual Research Report
  • [Presentation] マルチ亜鉛フィンガー型人工転写因子の創製と機能2009

    • Author(s)
      森崎達也、今西未来、二木史朗、杉浦幸雄
    • Organizer
      第129回日本薬学会
    • Place of Presentation
      京都市
    • Year and Date
      2009-03-10
    • Related Report
      2010 Final Research Report
  • [Presentation] マルチ亜鉛フィンガーのin vitro及びin celluloでの挙動相関2008

    • Author(s)
      森崎達也、今西未来、二木史朗、杉浦幸雄
    • Organizer
      第31回日本分子生物学会年会
    • Place of Presentation
      神戸市
    • Year and Date
      2008-12-09
    • Related Report
      2010 Final Research Report
  • [Presentation] 新機能性人工亜鉛フィンガータンパク質の設計2008

    • Author(s)
      杉浦幸雄
    • Organizer
      第8回日本蛋白質科学会
    • Place of Presentation
      東京都
    • Year and Date
      2008-06-18
    • Related Report
      2010 Final Research Report
  • [Presentation] 新機能性人工亜鉛フィンガー蛋白質の設計2008

    • Author(s)
      杉浦幸雄
    • Organizer
      第8回蛋白質科学会年会
    • Place of Presentation
      タワーホール船橋(東京)
    • Year and Date
      2008-06-10
    • Related Report
      2008 Annual Research Report
  • [Book] Engineered Zinc Figer Proteins(Eds.by J.P.Mackey, D.J.Segal)(Non-FolkI-based zinc finger nucleases)2010

    • Author(s)
      M.Imanishi, S.Negi, Y.Sugiura
    • Publisher
      Springer
    • Related Report
      2010 Final Research Report
  • [Book] Engineered Zinc Finger Proteins2010

    • Author(s)
      M, Imanishi
    • Publisher
      Springer Science
    • Related Report
      2010 Annual Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

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