Identification and functional analysis of factors related to human herpesvirus pathogenesis

• Project/Area Number: 20390138
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Virology
• Research Institution: Kobe University
• Principal Investigator: MORI Yasuko Kobe University, 大学院・医学研究科, 教授 (50343257)
• Co-Investigator(Kenkyū-buntansha): OKAMOTO Shigefumi 医薬基盤研究所, 創薬基盤研究部, プロジェクトリーダー (50311759) ; TAKEMOTO Masaya 医薬基盤研究所, 基盤研究部, 研究員 (60379237)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥20,020,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥4,620,000); Fiscal Year 2010: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2009: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2008: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
• Keywords: 遺伝子発現 / 細胞向性 / ウイルス感染 / 病原性

Research Abstract

HHV-6 is T lympho-tropic virus and causes several diseases by reactivation, but it is still unknown regarding the pathogenesis. Here we successfully established the construction system of HHV-6 recombinant virus that had not been established yet. We found that envelope glycoprotein gQ1 that is HHV-6 specific gene is essential for virus growth. Moreover, we found that HHV-6 is captured and transmitted to T cells by DC, and can replicate in T cells completely.

Research Products

• [Journal Article] Human herpesvirus 6 encoded glycoprotein Q1 gene is essential for virus growth. (2010)
  • Author(s): Tang H, Kawabata A, Yoshida M, Oyaizu H, Maeki T, Yamanishi K, Mori Y.
  • Journal Title: Virology
  • Peer Reviewed
• [Journal Article] Human herpesvirus-6 entry into host cells. (2010)
  • Author(s): Tang H, Mori Y.
  • Journal Title: Future Microbiol. 5(7) Pages: 1015-1023
  • Peer Reviewed
• [Journal Article] Characterization of varicella-zoster virus-encoded ORF0 gene-comparison of parental and vaccine strains. (2010)
  • Author(s): Koshizuka T, Ota M, Yamanishi K, Mori Y.
  • Journal Title: Virology. 30;405(2) Pages: 280-288
  • Peer Reviewed
• [Journal Article] Characterization of the varicella-zoster virus ORF50 gene, which encodes glycoprotein M. (2010)
  • Author(s): Sadaoka T, Yanagi T, Yamanishi K, Mori Y.
  • Journal Title: J Virol. 84(7) Pages: 3488-3502
  • Peer Reviewed
• [Journal Article] ヒトヘルペスウイルス6とヒトヘルペスウイルス7(HHV-6,HHV-7) (2010)
  • Author(s): 湯華民, 定岡知彦, 森康子
  • Journal Title: ウイルス(日本ウイルス学会誌) Volume: 60(2) Pages: 221-236
  • NAID: 10027644609
• [Journal Article] Human herpesvirus 6 encoded glycoprotein Q1 gene is essential for virus growth. (2010)
  • Author(s): Tang H, Kawabata A, Yoshida M, Oyaizu H, Maeki T, Yamanishi K, Mori Y
  • Journal Title: Virology. Volume: 407(2) Pages: 360-7
  • Peer Reviewed
• [Journal Article] Characterization of the varicella-zoster virus ORF50 gene, which encodes glycoprotein M (2010)
  • Author(s): Sadaoka T, Yanagi T, Yamanishi K, Mori Y.
  • Journal Title: J.Virology 84 Pages: 3488-3502
  • Peer Reviewed
• [Journal Article] Myelin-associated glycoprotein mediates membrane fusion and entry of neurotropic herpesviruses (2010)
  • Author(s): Suenaga T, Satoh T, Somboonthum P, Kawaguchi Y, Mori Y, Arase H.
  • Journal Title: Proc.Natl.Acad.Sci.USA. 107 Pages: 866-871
  • Peer Reviewed
• [Journal Article] Human herpesvirus 6 envelope components enriched in lipid rafts : evidence for virion-associated lipid rafts. (2009)
  • Author(s): Kawabata A, Tang H, Huang H, Yamanishi K, Mori Y.
  • Journal Title: Virol J. 19;6 Pages: 127-127
  • Peer Reviewed
• [Journal Article] Recent topics related to human herpesvirus 6 cell tropism. (2009)
  • Author(s): Mori Y.
  • Journal Title: Cell Microbiol 11(7) Pages: 1001-1006
  • Peer Reviewed
• [Journal Article] Role of dendritic cells infected with human herpesvirus 6 in virus transmission to CD4(+) T cells. (2009)
  • Author(s): Takemoto M, Imasawa T, Yamanishi K, Mori Y.
  • Journal Title: Virology. 15;385(2) Pages: 294-302
  • Peer Reviewed
• [Journal Article] Human herpesvirus 6 envelope components enriched in lipid rafts : evidence for virion-associated lipid rafts (2009)
  • Author(s): Kawabata A, Tang H, Huang H, Yamanishi K, Mori Y.
  • Journal Title: Virol.J. 6 Pages: 1-7
  • Peer Reviewed
• [Journal Article] Recent topics related to human herpesvirus 6 cell tropism (2009)
  • Author(s): Mori Y.
  • Journal Title: Cell.Microbiol. 11 Pages: 1001-1006
  • Peer Reviewed
• [Journal Article] Role of dendritic cells infected with human herpesvirus 6 in virus transmission to CD4(+)T cells (2009)
  • Author(s): Takemoto M, Imasawa T, Yamanishi K, Mori Y.
  • Journal Title: Virology 385 Pages: 294-302
  • Peer Reviewed
• [Journal Article]
  • Peer Reviewed
• [Journal Article]
  • Peer Reviewed
• [Journal Article]
  • Peer Reviewed
• [Presentation] The Functional Analysis Of Glycoprotein O During HHV-6 Infection : HHV-6 gO is Non- Essential for the Virus Growth in CBMCs. (2011)
  • Author(s): H.Tang, H.Oyaizu, T.Maeki, K.Yamanishi, Y.Mori
  • Organizer: 7th International Conference on HHV-6 & 7.
  • Place of Presentation: Reston, Virginia, USA
• [Presentation] Identification of the Epitopes on Neutralizing MAb for Human Herpesvirus 6 Glucoprotein Q1. (2011)
  • Author(s): A.Kawabata, E.Hayashi, M.Hayashi, H.Oyaizu, H.Tang, K.Yamanishi, Y.Mori
  • Organizer: 7th International Conference on HHV-6 & 7.
  • Place of Presentation: Reston, Virginia, USA
• [Presentation] HHV-6 Glycoprotein Complex Formation is Required for the Folding and Trafficking of the Complex, gH/gL/gQ1/ gQ2 and its Cellular Receptor Binding (2010)
  • Author(s): 森康子
  • Organizer: gQ1.7th International Conference on HHV-6 & 7.
  • Place of Presentation: Reston, Virginia, USA
• [Presentation] ヒトヘルペスウイルス6glycoprotein Q1に対する中和抗体の作製とその解析 (2010)
  • Author(s): 河端暁子、林麻佑子、小柳津裕子、湯華民、山西弘一、森康子
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: 徳島県徳島市
• [Presentation] Human herpesvirus-6 encoded glycoprotein Q1 gene is essential for virus growth. (2010)
  • Author(s): Huamin Tang, Akiko Kawabata, Hiroko Oyaizu, Takahiro Maeki, Koichi Yamanishi, Yasuko Mori
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: 徳島県徳島市
• [Presentation] ヒトヘルペスウイルス6(HHV-6)がコードするテグメントタンパクU14と細胞周期の関連性 (2010)
  • Author(s): 小柳津裕子、忠垣憲次郎、湯華民, 黒田和道、水口裕之、山西弘一、森康子
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: 徳島県徳島市
• [Presentation] HHV-6 glycoprotein complex formation is required for the folding and 3.05 trafficking of the complex, gH/gL/gQ1/ gQ2 and its cellular receptor binding. (2010)
  • Author(s): Tang H, Oyaizu H, Yamanishi K, Mori Y
  • Organizer: 35th International Herpesvirus Workshop.
  • Place of Presentation: Salt Lake City, Utah, USA
• [Presentation] ヘルペスウイルス感染における神経組織指向性レセプター (2010)
  • Author(s): 末永忠弘、有澤史倫、佐藤毅史、Pranee Somboonthum、森康子、川口寧、荒瀬尚
  • Organizer: 第25回ヘルペスウイルス研究会
  • Place of Presentation: 静岡県浜松市
• [Presentation] ヒトヘルペスウイルス6がコードする糖タンパク複合体gH/gL/gQ1/gQ2の解析 (2010)
  • Author(s): 湯華民、林麻佑子、山西弘一、森康子
  • Organizer: 第25回ヘルペスウイルス研究会
  • Place of Presentation: 静岡県浜松市
• [Presentation] ヒトヘルペスウイルス6がコードする糖タンパクglycoproteinQ(gQ)に対するモノクローナル抗体作製 (2010)
  • Author(s): 林麻佑子、湯華民、山西弘一、森康子
  • Organizer: 第25回ヘルペスウイルス研究会
  • Place of Presentation: 静岡県浜松市
• [Presentation] 水痘帯状疱疹ウイルスORF44の解析 (2010)
  • Author(s): 定岡知彦、林麻佑子、山西弘一、森康子
  • Organizer: 第25回ヘルペスウイルス研究会
  • Place of Presentation: 静岡県浜松市
• [Presentation] ヒトヘルペスウイルスglycoprotein Mと相互作用する宿主因子の解析 (2010)
  • Author(s): 河端暁子、世良田聡、仲哲治、山西弘一、森康子
  • Organizer: 第25回ヘルペスウイルス研究会
  • Place of Presentation: 静岡県浜松市
• [Presentation] VZV glycoprotein M and glycoprotein N assist each other for their functions (2009)
  • Author(s): Sadaoka, T., Yamanishi K., Mori, Y.
  • Organizer: The 14^<th> International Conference on Immunobiology and Prophylaxis of Human Herpesvirus Infections
  • Place of Presentation: クラウンプラザ神戸(兵庫県)
  • Year and Date: 2009-10-07
• [Presentation] Human herpesvirus-6 gH/gL/gQ complex and its receptor usage (2009)
  • Author(s): Mori, Y.
  • Organizer: The 14^<th> International Conference on Immunobiology and Prophylaxis of Human Herpesvirus Infections
  • Place of Presentation: クラウンプラザ神戸(兵庫県)
  • Year and Date: 2009-10-06
• [Presentation] Comparison and characterization of VZV encoded ORFO of the parental and vaccine strains (2009)
  • Author(s): Koshizuka, T., Yamanishi, K., Mori, Y.
  • Organizer: 34^<th> International Herpesvirus Workshop
  • Place of Presentation: アメリカ イサカ
  • Year and Date: 2009-07-25
• [Presentation] Newly identified mRNA arisen from VZV ORF50 gene region was not essential for viral growth (2009)
  • Author(s): Sadaoka, T., Yamanishi, K., Mori, Y.
  • Organizer: 34^<th> International Herpesvirus Workshop
  • Place of Presentation: アメリカ イサカ
  • Year and Date: 2009-07-25
• [Presentation] ヒトヘルペスウイルス6特異的エンベロープ糖タンパクをコードするgQ遺伝子の解析 (2009)
  • Author(s): 湯華民、河端暁子、山西弘一、森康子
  • Organizer: 第57回日本ウイルス学会学術集会
  • Place of Presentation: 東京都千代田区
• [Presentation] 水痘帯状疱疹ウイルスワクチン株ORF0は糖タンパク質である (2009)
  • Author(s): 腰塚哲朗、山西弘一、森康子
  • Organizer: 第57回日本ウイルス学会学術集会
  • Place of Presentation: 東京都千代田区
• [Presentation] 水痘帯状疱疹ウイルスglycoprotein Mの成熟機構の解析 (2009)
  • Author(s): 定岡知彦、山西弘一、森康子
  • Organizer: 第57回日本ウイルス学会学術集会
  • Place of Presentation: 東京都千代田区
• [Presentation] ヒトヘルペスウイルス6 glycoprotein Mと相互作用する宿主因子の同定および解析 (2009)
  • Author(s): 河端暁子、山西弘一、森康子
  • Organizer: 第57回日本ウイルス学会学術集会
  • Place of Presentation: 東京都千代田区
• [Presentation] Human herpesvirus-6 gH/gL/gQ complex and its receptor usage. (2009)
  • Author(s): 森康子
  • Organizer: The 14th International Conference on Immunobiology and Prophylaxis of Human Herpesvirus Infections.
  • Place of Presentation: Kobe, japan
• [Presentation] The ORF0 protein of varicella zoster virus vaccine strain is N-glycosylated. (2009)
  • Author(s): Koshizuka, T., Yamanishi K., Mori Y.
  • Organizer: The 14th International Conference on Immunobiology and Prophylaxis of Human Herpesvirus Infections.
  • Place of Presentation: Kobe, japan
• [Presentation] Decreased varicella zoster virus-specific cell-mediated immunity in patients with diabetes mellitus. (2009)
  • Author(s): Okamoto, S., Hata, A., Sadaoka, K., Yamanishi K., Mori Y.
  • Organizer: 34th International Herpesvirus Workshop.
  • Place of Presentation: Ithaca, NY, USA
• [Presentation] 水痘帯状疱疹ウイルスglycoprotein Mの詳細な解析 (2009)
  • Author(s): 定岡知彦、山西弘一、森康子
  • Organizer: 第24回ヘルペスウイルス研究会
  • Place of Presentation: 静岡県裾野市