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Clinical application of a novel rapid gene analyzing method aiming at personalized medicine

Research Project

Project/Area Number 20390164
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory medicine
Research InstitutionGunma University

Principal Investigator

MURAKAMI Masami  Gunma University, 大学院・医学系研究科, 教授 (30241871)

Co-Investigator(Kenkyū-buntansha) YAMAMOTO Koujirou  群馬大学, 大学院・医学系研究科, 教授 (70174787)
NARA Makoto  群馬大学, 大学院・医学系研究科, 助教 (80420165)
OGIWARA Takayuki  群馬大学, 医学部, 助教 (80361377)
MORIMURA Tadashi  群馬大学, 医学部, 助教 (20436306)
TSUNEKAWA Katsuhiko  群馬大学, 大学院・医学系研究科, 助教 (30436307)
Co-Investigator(Renkei-kenkyūsha) LEZHAVA Alexander  独立行政法人理化学研究所, ゲノム科学総合研究センター, 専任研究員 (40443048)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥15,470,000 (Direct Cost: ¥11,900,000、Indirect Cost: ¥3,570,000)
Fiscal Year 2010: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2009: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2008: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Keywords遺伝子 / 一塩基多型 / 糖尿病 / 肥満 / 薬剤反応性 / バイオテクノロジー
Research Abstract

In order to introduce personalized medicine, single nucleotide polymorphism of drug metabolizing enzymes in the warfarin treated patients and energy metabolism related proteins in the patients with diabetes and obesity using a novel rapid gene analyzing technique, the Smart Amplification Process.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (50 results)

All 2011 2010 2009 2008

All Journal Article (34 results) (of which Peer Reviewed: 32 results) Presentation (10 results) Book (4 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 1 results)

  • [Journal Article] Derivatives of Dictyostelium differentiation-inducing factors promote mitogen-activated IL-2 production via AP-1 in Jurkat cells.2011

    • Author(s)
      Takahashi K, Murakami M, Kikuchi H, Oshima Y, Kubohara Y
    • Journal Title

      Life Sci 488

      Pages: 480-485

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Derivatives of Dictyostelium differentiation-inducing factors promote mitogen-activated IL-2 production via AP-1 in Jurkat cells.2011

    • Author(s)
      Takahashi K, et al.
    • Journal Title

      Life Sci

      Volume: 88 Pages: 480-485

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mechanism and role of high-density lipoprotein-induced activation of AMP-activated protein kinase in endothelial cells.2010

    • Author(s)
      Kimura T, Tomura H, Sato K, Ito M, Matsuoka I, Im DS, Kuwabara A, Mogi C, Itch H, Kurose H, Murakami M, Okajima F
    • Journal Title

      J Biol Chem 285

      Pages: 4387-4397

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Oxidative stress associated with rapid weight reduction decreases circulating adiponectin concentrations.2010

    • Author(s)
      Yanagawa Y, Morimura T, Tsunekawa K, Seki K, Ogiwara T, Kotajima N, Machida T, Matsumoto S, Adachi T, Murakami M
    • Journal Title

      Endocr J 57

      Pages: 339-345

    • NAID

      10029584410

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effects of raloxifene on brachial arterial endothelial function, carotid wall thickness, and arterial stiffness in osteoporotic postmenopausal women.2010

    • Author(s)
      Sumino H, Ichikawa S, Kasama S, Takahashi T, Sakamoto H, Koizumi A, Kanai H, Araki Y, Itch T, Iwasaki T, Sawada Y, Saito Y, Kumakura H, Takayama Y, Kanda T, Murakami M, Sakamaki T, Kurabayashi M
    • Journal Title

      Int Heart J 51

      Pages: 60-67

    • NAID

      130000162398

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effects of raloxifene on the renin-angiotensin-aldosterone system and blood pressure in hypertensive and normotensive osteoporotic postmenopausal women.2010

    • Author(s)
      Sumino H, Ichikawa S, Kasama S, Takahashi T, Kumakura H, Takayama Y, Kanda T, Murakami M, Kurabayashi M
    • Journal Title

      Geriatr Gerontol Int 10

      Pages: 70-77

    • NAID

      10027271704

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Increased type 3 iodothyronine deiodinase activity in a regrown hepatic hemangioma with consumptive hypothyroidism.2010

    • Author(s)
      Bessho K, Etani Y, Ichimori H, Miyoshi Y, Namba N, Yoneda A, Ooue T, Chihara T, Morii E, Aoki T, Murakami M, Mushiake S, Ozono K
    • Journal Title

      Eur J Pediatr 169

      Pages: 215-221

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Influence of Thyroid Hormones and Transforming Growth Factor-β1 on Cystatin C Concentrations.2010

    • Author(s)
      Kotajima N, Yanagawa Y, Aoki T, Tsunekawa K, Morimura T, Ogiwara T, Nara M, Murakami M
    • Journal Title

      J Int Med Res 38

      Pages: 1365-1373

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Blood rheology and the low-density lipoprotein holesterol/high-density lipoprotein cholesterol ratio in dyslipidaemic and normolipidaemic subjects.2010

    • Author(s)
      Machida T, Sumino H, Fukushima M, Kotajima N, Amagai H, Murakami M
    • Journal Title

      J Int Med Res 38

      Pages: 1975-1984

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mechanism and role of high-density lipoprotein-induced activation of AMP-activated protein kinase in endothelial cells.2010

    • Author(s)
      Kimura T, et al.
    • Journal Title

      J Biol Chem

      Volume: 285 Pages: 4387-4397

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of raloxifene on brachial arterial endothelial function, carotid wall thickness, and arterial stiffness in osteoporotic postmenopausal women.2010

    • Author(s)
      Sumino H, et al.
    • Journal Title

      Int Heart J

      Volume: 51 Pages: 60-67

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of raloxifene on the renin-angiotensin-aldosterone system and blood pressure in hypertensive and normotensive osteoporotic postmen opausal women.2010

    • Author(s)
      Sumino H, et al.
    • Journal Title

      Geriatr Gerontol Int

      Volume: 10 Pages: 70-77

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Influence of thyroid hormones and transforming growth factor-β1 on cystatin C concentrations.2010

    • Author(s)
      Kotajima N, et al.
    • Journal Title

      J Int Med Res

      Volume: 38 Pages: 1365-1372

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Blood theology and the low-density lipoprotein cholesterol/high-density lipoprotein cholesterol ratio in dyslipidaemic and normolipidaemic subiects.2010

    • Author(s)
      Machida T, et al.
    • Journal Title

      J Int Med Res

      Volume: 38 Pages: 1975-1984

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Oxidative stress associated with rapid weight reduction decreases circulating adiponectin concentrations.2010

    • Author(s)
      Yanagawa Y, et al.
    • Journal Title

      Endocrine J 57

      Pages: 339-345

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Increased type 3 iodothyronine deiodinase activity in a regrown hepatic hemangioma with consumptive hypothyroidism.2010

    • Author(s)
      Bessho K, et al.
    • Journal Title

      Eur J Pediatr 169

      Pages: 215-221

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] メタボリックシンドロームのSNP診断2010

    • Author(s)
      常川勝彦、村上正巳
    • Journal Title

      Medical Bio 7

      Pages: 36-41

    • Related Report
      2009 Annual Research Report
  • [Journal Article] Rapid single-nucleotide polymorphism detection of cytochrome P450 (CYP2C9) and vitamin K epoxide reductase (VKORC1) genes for the warfarin dose adjustment by the SMart-amplification process version 2.2009

    • Author(s)
      Aomori T, Yamamoto K, Oguchi-Katayama A, Kawai Y, Ishidao T, Mitani Y, Kogo Y, Lezhava A, Fujita Y, Obayashi K, Nakamura K, Kohnke H, Wadelius M, Ekstrom L, Skogastierna C, Rane A, Kurabayashi M, Murakami M, Cizdziel PE, Hayashizaki Y, Horiuchi R
    • Journal Title

      Clin Chem 55

      Pages: 804-812

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Regular physical exercise improves physical motor functions and biochemical markers in middle-age and elderly women.2009

    • Author(s)
      Kamijo T, Murakami M
    • Journal Title

      J Phys Act Health 6

      Pages: 55-62

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Impaired blood rheology and elevated remnant-like lipoprotein particle cholesterol in hypercholesterolaemic subjects.2009

    • Author(s)
      Nara M, Sumino H, Nara M, Machida T, Amagai H, Nakajima K, Murakami M
    • Journal Title

      J Int Med Res 37

      Pages: 308-317

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Regulation of IL-2 production in Jurkat cells by Dictyostelium-derived factors.2009

    • Author(s)
      Takahashi K, Murakami M, Hosaka K, Kikuchi H, Oshima Y, Kubohara Y
    • Journal Title

      Life Sci 85

      Pages: 438-443

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Selenium enrichment of broccoli sprout extract increases chemosensitivity and apoptosis of LNCaP prostate cancer cells.2009

    • Author(s)
      Abdulah R, Faried A, Kobayashi K, Yamazaki C, Suradji EW, Ito K, Suzuki K, Murakami M, Kuwano H, Koyama H
    • Journal Title

      BMC Cancer 9

      Pages: 414-414

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Type 2 diabetes mellitus accompanied by isolated adrenocorticotropic hormone deficiency and gastric cancer.2009

    • Author(s)
      Kamiya Y, Murakami M
    • Journal Title

      Intern Med 48

      Pages: 1031-1035

    • NAID

      130000121902

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Decreased vitreal hyaluronan levels with aging.2009

    • Author(s)
      Itakura H, Kishi S, Kotajima N, Murakami M
    • Journal Title

      Ophthalmologica 223

      Pages: 32-35

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Impaired blood rheology and elevated remnant-like lipoprotein particle cholesterol in hypercholesterolaemic subjects.2009

    • Author(s)
      Nara M, et al.
    • Journal Title

      J Int Med Res 37

      Pages: 308-317

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Regulation of IL-2 production in Jurkat cells by Dictyostelium-derived factors.2009

    • Author(s)
      Takahashi K, et al.
    • Journal Title

      Life Sci 85

      Pages: 438-443

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rapid single-nucleotide polymorphism detection of cytochrome P450 (CYP2C9) and vitamin K epoxide reductase (VKORC1) genes for the warfarin dose adjustment by the SMart-amplification process version 2.2009

    • Author(s)
      Aomori T et al.
    • Journal Title

      Clin Chem 55

      Pages: 804-812

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Regular physical exercise improves physical motor functions and biochemical markers in middle-age and elderly women.2009

    • Author(s)
      Kmijo T et al.
    • Journal Title

      J Phys Act Health 6

      Pages: 55-62

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of raloxifene and hormone replacement therapy on forearm skin elasticity in postmenopausal women2009

    • Author(s)
      Sumino H et al.
    • Journal Title

      Maturitas 62

      Pages: 53-57

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Induction of scavenger receptor class B type I is critical for simvastatin enhancement of high-density lipoprotein-induced anti-inflammatory actions in endothelial cells.2008

    • Author(s)
      Kimura T, Mogi C, Tomura H, Kuwabara A, Im DS, Sato K, Kurose H, Murakami M, Okajima F
    • Journal Title

      J Immunol 181

      Pages: 7332-7340

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Relationship between carotid atherosclerosis and lumbar spine bone mineral density in postmenopausal women.2008

    • Author(s)
      Sumino H, Ichikawa S, Kasama S, Takahashi T, Sakamoto H, Kumakura H, Takayama Y, Kanda T, Murakami M, Kurabayashi M
    • Journal Title

      Hypertens Res 31

      Pages: 1191-1197

    • NAID

      10021936685

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Relationship between carotid atherosclerosis and lumbar spine bone mineral density in postmenopausal women.2008

    • Author(s)
      Sumino H et al.
    • Journal Title

      Hypertens Res 31

      Pages: 1191-1197

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Induction of scavenger receptor class B type I is critical for simvastatin enhancement of high-density lipoprotein-induced anti-inflammatory actions in endothelial cells.2008

    • Author(s)
      Kimura T et al.
    • Journal Title

      J Immunol 181

      Pages: 7332-7340

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 肥満における遺伝子解析の異議と臨床応用2008

    • Author(s)
      常川勝彦、村上正巳
    • Journal Title

      Human Science 19

      Pages: 26-29

    • Related Report
      2008 Annual Research Report
  • [Presentation] 甲状腺ホルモン代謝調節と疾患2011

    • Author(s)
      村上正巳
    • Organizer
      第11回日本内分泌学会関東甲信越支部学術集会
    • Place of Presentation
      横浜
    • Year and Date
      2011-03-05
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] 糖尿病の診断基準とHbAlcの標準化2010

    • Author(s)
      村上正巳
    • Organizer
      第47回日本臨床生理学会総会
    • Place of Presentation
      前橋
    • Year and Date
      2010-11-20
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] 臨床検体の研究利用における倫理的問題について2010

    • Author(s)
      村上正巳
    • Organizer
      第57回日本臨床検査医学会学術集会
    • Place of Presentation
      東京
    • Year and Date
      2010-09-11
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] 甲状腺疾患と臨床検査2010

    • Author(s)
      村上正巳
    • Organizer
      第17回軽井沢免疫セミナー
    • Place of Presentation
      軽井沢
    • Year and Date
      2010-06-12
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] 甲状腺ホルモン脱ヨード酵素と疾患2010

    • Author(s)
      村上正巳
    • Organizer
      日本内分泌学会
    • Place of Presentation
      前橋
    • Year and Date
      2010-04-23
    • Related Report
      2009 Annual Research Report
  • [Presentation] 新規迅速遺伝子解析技術SMAP法を用いた肥満関連遺伝子多型と内臓脂肪との関連性の検討2009

    • Author(s)
      常川勝彦、村上正巳
    • Organizer
      第56回日本臨床検査医学会学術集会
    • Place of Presentation
      札幌
    • Year and Date
      2009-08-27
    • Related Report
      2010 Final Research Report
  • [Presentation] 新規迅速遺伝子解析技術SMAP法を用いた肥満関連遺伝子多型と内臓脂肪との関連性の検討2009

    • Author(s)
      常川勝彦
    • Organizer
      日本臨床検査医学会
    • Place of Presentation
      札幌
    • Year and Date
      2009-08-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] 甲状腺ホルモン脱ヨード酵素と疾患2009

    • Author(s)
      村上正巳
    • Organizer
      第82回日本内分泌学会学術集会
    • Place of Presentation
      前橋
    • Year and Date
      2009-04-23
    • Related Report
      2010 Final Research Report
  • [Presentation] 地域住民における2型甲状腺ホルモン脱ヨード酵素遺伝子多型の糖代謝に及ぼす影響の検討2008

    • Author(s)
      常川勝彦、村上正巳
    • Organizer
      第55回日本臨床検査医学会学術集会
    • Place of Presentation
      名古屋
    • Year and Date
      2008-11-29
    • Related Report
      2010 Final Research Report
  • [Presentation] 地域住民における2型甲状腺ホルモン脱ヲード酵素遺伝子多型の糖代謝に及ぼす影響の検討.2008

    • Author(s)
      常川勝彦ら
    • Organizer
      第55回日本臨床検査医学会学術集会
    • Place of Presentation
      名古屋
    • Year and Date
      2008-11-29
    • Related Report
      2008 Annual Research Report
  • [Book] 今日の臨床検査2011

    • Author(s)
      村上正巳
    • Total Pages
      625
    • Publisher
      南江堂
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Book] パーフェクトガイド検査値事典2011

    • Author(s)
      村上正巳
    • Total Pages
      725
    • Publisher
      総合医学社
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Book] 甲状腺疾患と自己抗体検査2010

    • Author(s)
      村上正巳
    • Total Pages
      70
    • Publisher
      診断と治療社
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Book] 臨床病態学総論2009

    • Author(s)
      村上正巳
    • Total Pages
      282
    • Publisher
      ヌーヴェルヒロカワ
    • Related Report
      2010 Final Research Report
  • [Patent(Industrial Property Rights)] インターロイキン-2産生抑制剤2010

    • Inventor(s)
      久保原禅、村上正巳、高橋克典、大島吉輝、菊地晴久
    • Industrial Property Rights Holder
      群馬大学、東北大学
    • Filing Date
      2010-05-06
    • Related Report
      2010 Final Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] インターロイキン-2産生抑制剤2009

    • Inventor(s)
      久保原禅、村上正巳、高橋克典、大島吉輝、菊地晴久
    • Industrial Property Rights Holder
      群馬大学、東北大学
    • Filing Date
      2009-05-07
    • Related Report
      2010 Final Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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