Optimal therapeutic approach on ischemic brain insult using bone marrow stromal cells cultured under simulated microgravity

• Project/Area Number: 20390246
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurology
• Research Institution: Hiroshima University
• Principal Investigator: MATSUMOTO Masayasu Hiroshima University, 大学院・医歯薬学総合研究科, 教授 (20192346)
• Co-Investigator(Kenkyū-buntansha): YUGE Rui 広島大学, 保健学研究科, 教授 (20263676) ; OHTSUKI Toshiho 広島大学, 病院, 講師 (20418792) ; TAKAHASHI Tetsuya 広島大学, 病院, 講師 (00435942) ; YAMASHITA Hiroshi 広島大学, 病院, 講師 (20311813)
• Co-Investigator(Renkei-kenkyūsha): TAHARA Hidetoshi 広島大学, 医歯薬学総合研究科, 教授 (00271065)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000); Fiscal Year 2010: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2009: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2008: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
• Keywords: 脳神経疾患 / 移植・再生医療 / 虚血性脳損傷 / 動物モデル

Research Abstract

The culture methods of stem cells are a key factor for tissue engineering and several methods have been proposed. We have showed that simulated microgravity allows novel LIF-free and animal derived material-free culture methods for mouse embryonic stem cells. Our study indicates that culturing cells under simulated microgravity enhances their survival rate by maintaining an undifferentiated state of cells, making this a potentially attractive method for culturing donor cells to be used in grafting.

Research Products

• [Journal Article] Cerebral sinus thrombosis and heparin-induced thrombocytopenia in a patient with paroxysmal nocturnal hemoglobinuria. (2010)
  • Author(s): Ishihara-Kawase K, Ohtsuki T, Sugihara S, Tanaka H, Nakamura T, Kimura A, Matsumoto M.
  • Journal Title: Intern Med. 49 Pages: 941-943
  • NAID: 130000251567
  • Peer Reviewed
• [Journal Article] Trigeminal neuropathy from perineural spread of an amyloidoma detected by blink reflex and thin-slice magnetic resonance imaging. (2010)
  • Author(s): Yamazaki Y, Ochi K, Nakata Y, Dohi E, Eguchi K, Yamaguchi S, Matsushige T, Ueda T, Amatya VJ, Takeshima Y, Nakamura T, Ohtsuki T, Kohriyama T, Matsumoto M.
  • Journal Title: Muscle Nerve. 41 Pages: 875-878
  • Peer Reviewed
• [Journal Article] Positional relationship between recurrent intracerebral hemorrhage/lacunar infarction and previously detected microbleeds. (2010)
  • Author(s): Sueda Y, Naka H, Ohtsuki T, Kono T, Aoki S, Ohshita T, Nomura E, Wakabayashi S, Kohriyama T, Matsumoto M.
  • Journal Title: AJNR Am J Neuroradiol. 31 Pages: 1498-1503
  • Peer Reviewed
• [Journal Article] The pathophysiology of prospective memory failure after diffuse axonal injury-lesion-symptom analysis using diffusion tensor imaging. (2010)
  • Author(s): Kondo K, Maruishi M, Ueno H, Sawada K, Hashimoto Y, Ohshita T, Takahashi T, Ohtsuki T, Matsumoto M.
  • Journal Title: BMC Neurosci 11 Pages: 147-147
  • NAID: 120002753723
  • Peer Reviewed
• [Journal Article] Yuge L, Sasaki A, Kawahara Y, Wu SL, Matsumoto M, Manabe T, Kajiume T, Takeda M, Magaki T, Takahashi T, Kurisu K, Matsumoto M. (2010)
  • Author(s): Yuge L, Sasaki A, Kawahara Y, Wu SL, Matsumoto M, Manabe T, Kajiume T, Takeda M, Magaki T, Takahashi T, Kurisu K, Matsumoto M.
  • Journal Title: 20 Pages: 893-900
  • Peer Reviewed
• [Journal Article] Cerebral sinus thrombosis and heparin-induced thrombocytopenia in a patient with paroxysmal nocturnal hemoglobinuria (2010)
  • Author(s): Ishihara-Kawase K, et al.
  • Journal Title: Intern Med. Volume: 49 Pages: 941-943
  • Peer Reviewed
• [Journal Article] Trigeminal neuropathy from perineural spread of an amyloidoma detected by blink reflex and thin-slice magnetic resonance imaging (2010)
  • Author(s): Yamazaki Y, et al.
  • Journal Title: Muscle Nerve Volume: 41 Pages: 875-878
  • Peer Reviewed
• [Journal Article] Positional relationship between recurrent intracerebral hemorrhage/lacunar infarction and previously detected microbleeds (2010)
  • Author(s): Sueda Y, et al.
  • Journal Title: AJNR Am J Neuroradiol Volume: 31 Pages: 1498-1503
  • Peer Reviewed
• [Journal Article] The pathophysiology of prospective memory failure after diffuse axonal injury--lesion-symptom analysis using diffusion tensor imaging (2010)
  • Author(s): Kondo K, et al.
  • Journal Title: BMC Neurosci Volume: 11
  • Peer Reviewed
• [Journal Article] Simulated Microgravity Maintains the Undifferentiated State and Enhances the Neural Repair Potential of Bone Marrow Stromal Cells (2010)
  • Author(s): Yuge L, et al.
  • Journal Title: Stem Cells Dev Volume: 20 Pages: 893-900
  • Peer Reviewed
• [Journal Article] Brain activations in errorless and errorful learning in patients with diffuse axonal injury : a functional MRI study. (2009)
  • Author(s): Ueno H, Maruishi M, Miyatani M, Muranaka H, Kondo K, Ohshita T, Matsumoto M.
  • Journal Title: Brain Inj. 23 Pages: 291-298
  • NAID: 120001700399
  • Peer Reviewed
• [Journal Article] Association between central systolic blood pressure, white matter lesions in cerebral MRI and carotid atherosclerosis. (2009)
  • Author(s): Shrestha I, Takahashi T, Nomura E, Ohtsuki T, Ohshita T, Ueno H, Kohriyama T, Matsumoto M.
  • Journal Title: Hypertens Res 32 Pages: 869-874
  • NAID: 10026123715
  • Peer Reviewed
• [Journal Article] Diagonal ear-lobe crease is correlated with atherosclerotic changes in carotid arteries. (2009)
  • Author(s): Shrestha I, Ohtsuki T, Takahashi T, Nomura E, Kohriyama T, Matsumoto M.
  • Journal Title: Circ J. 73 Pages: 1945-1949
  • NAID: 10025936498
  • Peer Reviewed
• [Journal Article] LIF-free embryonic stem cell culture in simulated microgravity. (2009)
  • Author(s): Kawahara Y, Manabe T, Matsumoto M, Kajiume T, Matsumoto M, Yuge L.
  • Journal Title: PLoS One 23 Pages: 1-4
  • Peer Reviewed
• [Journal Article] Advantages of high b value diffusion-weighted imaging in the diagnosis of acute stroke-a case report. (2009)
  • Author(s): Doskaliyev A, Yamasaki F, Saito T, Nomura E, Sugiyama K, Ohtsuki T, Matsumoto M, Kurisu K.
  • Journal Title: Cerebrovasc Dis 27 Pages: 616-617
  • Peer Reviewed
• [Journal Article] Quantitative analysis of peripheral benzodiazepine receptor in the human brain using PET with (11) C-AC-5216. (2009)
  • Author(s): Miyoshi M, Ito H, Arakawa R, Takahashi H, Takano H, Higuchi M, Okumura M, Otsuka T, Kodaka F, Sekine M, Sasaki T, Fujie S, Seki C, Maeda J, Nakao R, Zhang MR, Fukumura T, Matsumoto M, Suhara T.
  • Journal Title: J Nucl Med 50 Pages: 1095-1101
  • Peer Reviewed
• [Journal Article] Association between central systolic blood pressure, white matter lesions in cerebral MRI and carotid atherosclerosis (2009)
  • Author(s): Shrestha I, et al.
  • Journal Title: Hypertens Res 32 Pages: 869-874
  • Peer Reviewed
• [Journal Article] Diagonal ear-lobe crease is correlated with atherosclerotic changes in carotid arteries (2009)
  • Author(s): Shrestha I, et al.
  • Journal Title: Circ J 73 Pages: 1945-1949
  • Peer Reviewed
• [Journal Article] LIF-free embryonic stem cell culture in simulated microgravity (2009)
  • Author(s): Kawahara Y
  • Journal Title: PLoS One 23 Pages: 1-4
  • Peer Reviewed
• [Journal Article] Quantitative analysis of peripheral benzodiazepine receptor in the human brain using PET with (11) C-AC-5216 (2009)
  • Author(s): Miyoshi M
  • Journal Title: J Nucl Med 50 Pages: 1095-1101
  • Peer Reviewed
• [Journal Article] A Randomized, Double-Blind Study Comparing the Safety and Efficacy of Clopidogrel versus Ticlopidine in Japanese Patients with Noncardioembolic Cerebral Infarction. (2008)
  • Author(s): Fukuuchi Y, Tohgi H, Okudera T, Ikeda Y, Miyanaga Y, Uchiyama S, Hirano M, Shinohara Y, Matsumoto M, Yamaguchi T.
  • Journal Title: Cerebrovasc Dis. 25 Pages: 40-49
  • Peer Reviewed
• [Journal Article] Abundance of aspargynyl-hydroxylase FIH is regulated by Siah-1 under normoxic conditions. (2008)
  • Author(s): Fukuba H, Takahashi T, Jin HG, Kohriyama T, Matsumoto M.
  • Journal Title: Neurosci Lett 433 Pages: 209-214
  • Peer Reviewed
• [Journal Article] Synphilin-1 transgenic mice exhibit mild motor impairments. (2008)
  • Author(s): Jin HG, Yamashita H, Nakamura T, Fukuba H, Takahashi T, Hiji M, Kohriyama T, Matsumoto M.
  • Journal Title: Neurosci Lett. 445 Pages: 12-17
  • Peer Reviewed
• [Journal Article] White matter lesions in the brain with frontotemporal lobar degeneration with motor neuron disease : TDP-43-immunopositive inclusions co-localize with p62, but not ubiquitin. (2008)
  • Author(s): Hiji M, Takahashi T, Fukuba H, Yamashita H, Kohriyama T, Matsumoto M.
  • Journal Title: Acta Neuropathol. 116 Pages: 183-191
  • Peer Reviewed
• [Journal Article] Association between cerebral microbleeds on T2*-weighted MR images and recurrent hemorrhagic stroke in patients treated with warfarin following ischemic stroke. (2008)
  • Author(s): Ueno H, Naka H, Ohshita T, Kondo K, Nomura E, Ohtsuki T, Kohriyama T, Wakabayashi S, Matsumoto M.
  • Journal Title: AJNR Am J Neuroradio1. 29 Pages: 1483-1486
  • Peer Reviewed
• [Journal Article] Incidence of dementia, Alzheimer disease, and vascular dementia in a Japanese population : Radiation Effects ResearchFoundation adult health study. (2008)
  • Author(s): Yamada M, Mimori Y, Kasagi F, Miyachi T, Ohshita T, Sudoh S, Ikeda J, Matsui K, Nakamura S, Matsumoto M, Fujiwara S, Sasaki H.
  • Journal Title: Neuroepidemiology 30 Pages: 152-160
  • Peer Reviewed
• [Journal Article] Cytotoxic edema in neuro-Behcet's disease (2008)
  • Author(s): Shrestha I, Ohshita T, Naka H, Morino H, Matsumoto M.
  • Journal Title: Intern Med. 47 Pages: 2073-2076
  • NAID: 130000079804
  • Peer Reviewed
• [Journal Article] Association between cerebral microbleeds on T2^*-weighted MR images and recurrent hemorrhagic stroke in patients treated withwarfarin following ischemic stroke. (2008)
  • Author(s): Ueno, et al.
  • Journal Title: American Journal of Neuroradiology 29 Pages: 1483-1486
  • Peer Reviewed
• [Journal Article] Synphilin-1 transgenic mice exhibit mild motor impairments. (2008)
  • Author(s): Jin, et al.
  • Journal Title: Neurosci Lett. 445 Pages: 12-17
  • Peer Reviewed
• [Journal Article] White matter lesions in the brain with frontotemporal lobar degeneration with motor neuron disease : TDP-43-immunopositive inclusions co-localize with p62, but not ubiquitin. (2008)
  • Author(s): Hiji, et al.
  • Journal Title: Acta Neuropathol Pages: 183-191
  • Peer Reviewed
• [Presentation] CKDと脳血管障害～その予防と対策～ (2010)
  • Author(s): 松本昌泰
  • Organizer: 第40回日本腎臓学会西部学術大会
  • Place of Presentation: 広島国際会議場
• [Presentation] 頚動脈狭窄に対するベストメディカルトリートメント (2010)
  • Author(s): 松本昌泰
  • Organizer: 第9回日本頚部脳血管治療学会
  • Place of Presentation: 横浜(招待講演)
• [Presentation] CKDと脳血管障害～その予防と対策～ (2010)
  • Author(s): 松本昌泰
  • Organizer: 第40回日本腎臓学会西部学術大会
  • Place of Presentation: 広島(招待講演)
• [Presentation] 変貌する脳保護戦略 (2009)
  • Author(s): 松本昌泰
  • Organizer: 第62回日本自律神経学会総会
  • Place of Presentation: 和歌山
• [Presentation] 頚動脈病変評価:その臨床的意義 (2009)
  • Author(s): 松本昌泰
  • Organizer: 第50回日本脈管学会総会
  • Place of Presentation: 東京
• [Presentation] 変貌する脳卒中医療～チーム医療の意義～ (2009)
  • Author(s): 松本昌泰
  • Organizer: リハビリテーション・ケア合同研究大会広島2009
  • Place of Presentation: 広島
• [Presentation] メタボリックシンドロームと脳血管障害 (2009)
  • Author(s): 松本昌泰
  • Organizer: 第24回日本糖尿病合併症学会
  • Place of Presentation: 岡山
• [Presentation] 脳卒中医療維新のフロントライン (2009)
  • Author(s): 松本昌泰
  • Organizer: 第27回日本神経治療学会総会
  • Place of Presentation: 熊本
• [Presentation] 脳卒中医学・医療維新とEBM (2009)
  • Author(s): 松本昌泰
  • Organizer: 第18回日本脳ドック学会総会
  • Place of Presentation: 東京