| Project/Area Number |
20390456
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plastic surgery
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| Research Institution | Kobe University |
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Principal Investigator |
TERASHI Hiroto Kobe University, 医学研究科, 教授 (80217421)
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| Co-Investigator(Kenkyū-buntansha) |
TAHARA Shinya 神戸大学, 医学部附属病院, 教授 (60207206)
TERASHIMA Toshio 神戸大学, 医学研究科, 教授 (20101892)
FIJISATO Toshia 大阪工業大学, 工学研究科, 教授 (60270732)
HASHIKAWA Kazunobu 神戸大学, 医学部附属病院, 助教 (90403237)
SAKAKIBARA Shunsuke 神戸大学, 医学研究科, 特命助教 (50444592)
MAHARA Atsushi 国立循環器病センター, 先進医工学センター, 室員 (80416221)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000)
Fiscal Year 2010: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2008: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
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| Keywords | 神経 / 再生 / バイオマテリアル / 人工神経 / 再生医学 / 再生医療 / 末梢神経再建 / 再建外科 |
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| Research Abstract |
We developed novel decellularizing method using hypertonic sodium chloride solution. The histological evaluation for acellularization was performed by HE staining, immunostaining and electron microscope images. The acellular nerve was also transplanted to rats and evaluated histologically. The cells were washed out completely and extracellular matrix was maintained. Two months after the transplantation, Schwann cells thrust into the graft and neurofilament was running through the graft. This acellular nerve presents a useful scaffold for peripheral nerve regeneration.
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