| Project/Area Number |
20390511
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Niigata University |
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Principal Investigator |
SEO Kenji Niigata University, 医歯学系, 教授 (40242440)
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| Co-Investigator(Kenkyū-buntansha) |
FUJIWARA Naoshi 新潟大学, 医歯学系, 教授 (70181419)
MAEDA Takeyasu 新潟大学, 医歯学系, 教授 (40183941)
TSURUMAKI Tatsuru 新潟大学, 医歯学系, 助教 (10345522)
TSUKADA Hiroko 新潟大学, 医歯学総合病院, 医員 (30464019)
TANAKA Yutaka 新潟大学, 医歯学総合病院, 講師 (50323978)
YOSHIKAWA Hiroyuki 新潟大学, 医歯学総合病院, 医員 (20547575)
KURATA Shigenobu 新潟大学, 医歯学系, 助教 (20464018)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2010: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2009: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2008: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000)
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| Keywords | 歯科麻酔学 / GABAA receptor / muscimol / BDNF / Glia / trigeminal / subnucleus caudalis / nociception / membrane potential / γ2 subunit / PRIP-1 / PRIP-2 / double knock out / GABAA受容体 / γサブユニット / 三叉神経 / 侵害刺激 / MAC / 吸入麻酔薬 / イソフルラン / 逃避反射 |
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| Research Abstract |
Introduction ; This project aimed to investigate the possible involvement of GABAA receptor mediated nociceptive modulation in the spinal or trigeminal dorsal horn. We used PRIP-1, PRIP-2 double knockout mice (DKO) which deficits some GAGAA receptor function.
Method and results : Immnunohistochemical study exhibited a complete deficit in γ2 subunit of GABAA receptor on cell surface of the superficial and deep magnocellular layers in spinal cord of DKO mice. The MAC of wild or DKO animal was 1.36±0.04% (n=10) or 1.07±0.01% (n=10), respectively (Student's t-test, p<0.001). These results suggest that the stimulation of γ2 subunit might did not induce anti-nociceptive effect. Thirdly, spatial and temporal propagation of membrane potential and the changes in the intra- cellular calcium concentration in the slice of medulla exhibited the similar propagation between in the wild and DKO animals and furthermore a perfusion of the highly-selective GABAA receptor agonist muscimol did not induce any difference in these propagation features. The preconditioning by BDNF induced some difference in afferent stimulation induced changes in membrane potential propagation under muscimol perfusion.
Conclusion ; these results suggest that GABAA receptor does not have any significant role in modulation of nociceptive transmission in normal condition but some stimuli induce BDNF releasing might have some modulating effect on this transmission feature.
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