Visualization of changes in intracellular ions during glioma cell migration

• Project/Area Number: 20500335
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Hamamatsu University School of Medicine
• Principal Investigator: KUMADA Tatsuro Hamamatsu University School of Medicine, 医学部, 助教 (00402339)
• Co-Investigator(Renkei-kenkyūsha): FUKUDA Atsuo 浜松医科大学, 医学部, 教授 (50254272) ; FURUKAWA Tomonori 浜松医科大学, 医学部, 助教 (60402369) ; MORISHIMA Toshitaka 浜松医科大学, 医学部, 助教 (60456548)
• Research Collaborator: INOUE Koichi 浜松医科大学, 医学部, 准教授 (80345818)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: イメージング / グリオーマ / 細胞生理 / 細胞移動

Research Abstract

We monitored spontaneous changes in intracellular Ca^<2+> level and Cl^- during glioma cell migration using intracellular ion imaging and examined its roles. Although some cells which show marked acceleration of cell motility especially exhibited high frequency of spontaneous Ca2+ oscillation, the frequency of changes in intracellular Ca2+ levels was not correlated with the rate of cell movement. We also monitored the spatio-temporal dynamics of [Cl-]i by FRET imaging. Also, changes in intracellular Cl- levels can modify the cell motility of glioma cells.

Research Products

• [Journal Article] Inhibition of cerebellar granule cell turning by alcohol. (2010)
  • Author(s): Kumada, T., Komuro, Y., Li, Y., Hu, T., Wang, Z., Littner, Y., Komuro, H.
  • Journal Title: Neuroscience 170 Pages: 1328-1344
• [Journal Article] Dominant negative mutations in α-II spectrin cause early onset West syndrome with severe cerebral hypomyelination, spastic quadriplegia, and developmental delay. (2010)
  • Author(s): Saitsu, H., Tohyama, J., Kumada, T., Egawa, K., Hamada, K., Okada, I., Mizuguchi, T., Osaka, H., Miyata, R., Furukawa, T., Haginoya, K., Hoshino, H., Goto, T., Hachiya, Y., Yamagata, T., Saitoh, S., Nagai, T., Nishiyama, K., Nishimura, A., Miyake, N., Komada, M., Hayashi, K., Hirai, S., Ogata, K., Kato, M., Fukuda, A., Matsumoto, N.
  • Journal Title: Am.J.Human Genet. 86 Pages: 1-11
• [Journal Article] Possible contribution of 2-aminoethoxydiphenyl-borate-sensitive Ca(2+) mobilization to adrenocorticotropin-induced glucocorticoid synthesis in rat adrenocortical cells. (2010)
  • Author(s): Hayashi, C., Tino, K., Oki, Y., Matsushita, F., Yamashita, M., Yogo, K., Sasaki, S., Kumada, T., Nakamura, H.
  • Journal Title: Endocr.J. 57 Pages: 109-117
  • NAID: 10029583574
• [Journal Article] Dominant negative mutations in α-II spectrin cause early onset West syndrome with severe cerebral hypomyelination, spastic quadriplegia, and developmental delay. (2010)
  • Author(s): Saitsu, H., et al
  • Journal Title: Am.J.Human Genet. Volume: 86 Pages: 1-11
  • Peer Reviewed
• [Journal Article] Inhibition of cerebellar granule cell turning by alcohol. (2010)
  • Author(s): Kumada, T., et al
  • Journal Title: Neuroscience Volume: 170 Pages: 1328-1344
  • Peer Reviewed
• [Journal Article] Possible contribution of 2-Aminoethoxydiphenyl-borate-sensitive Ca (2+) mobilization to adrenocorticotropin-induced giuco corticoid synthesis in rat adrenocortical cells (2010)
  • Author(s): Hayashi, C., et al.
  • Journal Title: Endocr.J. 57 Pages: 109-17
  • Peer Reviewed
• [Journal Article] Autonomous turning of cerebellar granule cells in vitro by intrinsic programs. (2009)
  • Author(s): Kumada, T., Jiang, Y., Kawanami, A., Cameron, D.B., Komuro, H.
  • Journal Title: Dev Biol. 326 Pages: 237-349
• [Journal Article] Autonomous turning of cerebellar granule cells in vitro by intrinsic programs (2009)
  • Author(s): Kumada, T., et al.
  • Journal Title: Dev Biol. 326 Pages: 237-49
  • Peer Reviewed
• [Journal Article] Autonomous turning of cerebellar granule cells in vitro by intrinsic programs. (2009)
  • Author(s): Kumada T, et al
  • Journal Title: Dev. Biol. 326 Pages: 237-249
  • Peer Reviewed
• [Journal Article] Crucial roles of Robe proteins in midline crossing of cerebellofugal axons and lack of their up-regulation after midline crossing. (2008)
  • Author(s): Tamada, A*., Kumada, T*., Zhu, Y*., Matsumoto, T., Hatanaka, Y., Muguruma, K., Chen, Z., Tanabe, Y., Torigoe, M., Yamauchi, K., Oyama, H., Nishida, K., Murakami, F.
  • Journal Title: Neural Dev. 3 Pages: 29-29
• [Journal Article] De novo mutations in the gene encoding STXBP1 (MUNC18-1) cause early infantile epileptic encephalopathy. (2008)
  • Author(s): Saitsu, H., Kato, M., Mizuguchi, T., Hamada, K., Osaka, H., Tohyama, J., Uruno, K., Kumada, S., Nishiyama, K., Nishimura, A., Okada, I., Yoshimura, Y., Hirai, S., Kumada, T., Hayasaka, K., Fukuda, A., Ogata, K., Matsumoto, N.
  • Journal Title: Nat Genet. 40 Pages: 782-788
• [Journal Article] Involvement of ER stress via calcium homeostasis disturbance in A-beta accumulation in ARPE19 cells. (2008)
  • Author(s): Koyama, Y., Matsuzaki, S., Yamada, K., Katayama, T., Sato, K., Kumada, T., Fukuda, A., Matsuda, S., Gomi, F., Tone, Y., Tohyama, M.
  • Journal Title: Invest.Ophthalmol.Vis.Sci. 49 Pages: 2376-2383
• [Journal Article] Cerebellar granule cell migration and the effects of alcohol. (2008)
  • Author(s): Jiang, Y., Kumada, T., Cameron, D.B., Komuro, H.
  • Journal Title: Dev.Neurosci. 30 Pages: 7-23
• [Journal Article] De novo mutations in the gene encoding STXBP1(MUNC18-1)cause early infantile epileptic encephalopathy. (2008)
  • Author(s): Saitsu H, et al
  • Journal Title: Nat. Genet. 40 Pages: 782-788
  • Peer Reviewed
• [Journal Article] Induction of amyloid βacumulation by ER calcium disruption and resultant upregulation of angiogen in factorsin ARPE19 cells. (2008)
  • Author(s): Koyama H, et al
  • Journal Title: Ophthalmol. Visual Sci. 49 Pages: 2376-2383
  • Peer Reviewed
• [Journal Article] Crucial roles of Robo proteins in midline crossing of cerebellofugal axons and lack of their up-regulation after midline crossing. (2008)
  • Author(s): Tamada A, et al
  • Journal Title: Neural Dev. 3 Pages: 29-29
  • Peer Reviewed
• [Presentation] Roles of paracrine GABA and KCC2 downregulation in a model of focal cortical malformation. (2010)
  • Author(s): 福田敦夫, 王天英, 熊田竜郎, 森島寿貴, 柳川右千夫
  • Organizer: 第88回日本生理学会大会 第116回目本解剖学会総会・全国学術集会
  • Place of Presentation: 横浜
• [Presentation] Potential developmental regulation ofneuronal KCC2 activity by taurine. (2010)
  • Author(s): Inoue, K., Furukawa T., Yamada, J, Kumada, T., Wang, T., Fukuda, A.
  • Organizer: 第88回日本生理学会大会 第116回日本解剖学会総会・全国学術集会
  • Place of Presentation: 横浜
• [Presentation] Intracellular taurine inhibits KCC2 transporteractivity. (2010)
  • Author(s): Inoue, K., Furukawa T., Kumada T., Yamada, J., Wang T., Fukuda A.
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 神戸
• [Presentation] Proliferation of cerebellar granule cell precursors is promoted by GABA released from Bergmann glial cells. (2010)
  • Author(s): Fukuda A., Morishima T., Kumada T., Takayama, C., Yoshida, S.
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 神戸
• [Presentation] An origin and a Roles of ambient GABA during cerebellar development. (2010)
  • Author(s): Fukuda A., et al
  • Organizer: The 9th Korea-Japan Joint Symposium of Brain Sciences, and Cardiac and Smooth Muscles
  • Place of Presentation: Kagoshima
• [Presentation] Intracellular taurine inhibits KCC2 transporter activity. (2010)
  • Author(s): Inoue, K., et al
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 神戸
• [Presentation] Temporal release of GABA from bergmann glia regulates granule cell precursors proliferation during rat cerebellar cortical development. (2010)
  • Author(s): Morishima, T., et al
  • Organizer: 第29回内藤コンファレンス
  • Place of Presentation: 葉山
• [Presentation] タウリンによる神経特異的イオン輸送体KCC2の活性制御 (2010)
  • Author(s): 井上浩一, 他
  • Organizer: 第57回中部日本生理学会
  • Place of Presentation: 豊明市
• [Presentation] Temporally-increased GABA underlies the formation of freeze lesion-induced microgyrus and causes GABA_A receptor-mediated excitation. (2010)
  • Author(s): Wang, T., et al
  • Organizer: 第37回日本脳科学大会
  • Place of Presentation: 天津市
• [Presentation] グリオブラストーマ細胞の移動時で見られる特徴的な様式におけるクロライド輸送の役割 (2009)
  • Author(s): 熊田竜郎, 福田敦夫
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋
• [Presentation] 胎仔大脳皮質抑制性神経細胞の細胞新生が母体ストレスによって抑制される. (2009)
  • Author(s): 内田琢, 森島寿貴, 古川智範, 沖隆, 熊田竜郎, 柳川右千夫, 福田敦夫
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋
• [Presentation] GABAが小脳皮質形成期の外頼粒細胞層で一時的にバークマングリアから放出され穎粒細胞前駆体の増殖に関与している (2009)
  • Author(s): 森島寿貴, 熊田竜郎, 高山千利, 吉田祥子, 福田敦夫
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋
• [Presentation] 母体ストレスが及ぼすマウス胎仔大脳皮質形成の異常 (2009)
  • Author(s): 内田琢, 森島寿貴, 古川智範, 沖隆, 江川潔, 熊出竜郎, 柳川右千夫, 福出敦夫
  • Organizer: 第36回日本脳科学大会
  • Place of Presentation: 金沢
• [Presentation] Maternal stress decreased the generation of the GABAergic but not pyramidal neurons in the cerebral cortex (2009)
  • Author(s): 内田琢, et al.
  • Organizer: IUPS2009
  • Place of Presentation: Kyoto
• [Presentation] Role of [Ca^<2+>]_i and [Cl^-]_i alterations in distinct pattern of glioblastoma cell migration (2009)
  • Author(s): 熊田竜郎, et al.
  • Organizer: IUPS2009
  • Place of Presentation: Kyoto
• [Presentation] Temporal release of GABA from Bergmann glia accelerates granule cell precursors (GCPs) proliferation during rat cerebellar cortex development (2009)
  • Author(s): 森島寿貴, et al.
  • Organizer: IUPS2009
  • Place of Presentation: Kyoto
• [Presentation] Peculiar accumulation of GABAergic and glutamatergic neurons at the early stage of freeze lesion-induced microgyrus in mice (2009)
  • Author(s): Wang, T., et al.
  • Organizer: IUPS2009
  • Place of Presentation: Kyoto
• [Presentation] Endogenous taurine tonically activates GABAA receptors in embryonic mouse neocortex (2009)
  • Author(s): Fukuda, A., et al.
  • Organizer: PAT-CVR 2009
  • Place of Presentation: Okazaki
• [Presentation] Temporal release of GABA regulates granule cell precursors (GCPs) proliferation during rat cerebellar cortical development (2009)
  • Author(s): 森島寿貴, et al.
  • Organizer: PAT-CVR 2009
  • Place of Presentation: Okazaki
• [Presentation] [Ca^<2+>]_i and [Cl^-]_i alterations in migrating glioblastoma cells (2009)
  • Author(s): 熊田竜郎, et al.
  • Organizer: PAT-CVR 2009
  • Place of Presentation: Okazaki
• [Presentation] Neurogenesis of GABAergic interneurons but not of cortical plate cells in the fetal cerebral cortex was decreased by maternal stress (2009)
  • Author(s): 内田琢, et al.
  • Organizer: PAT-CVR 2009
  • Place of Presentation: Okazaki
• [Presentation] Temporal release of GABA from Bergman glia (BG) regulates granule cell precursors (GCPs) proliferation during rat cerebellar cortical development (2009)
  • Author(s): 森島寿貴, et al.
  • Organizer: PAT-CVR 2009
  • Place of Presentation: Okazaki
• [Presentation] GABAが小脳皮質形成期の外顆粒細胞層で一時的にバークマングリアから放出され顆粒細胞前駆体の増殖に関与している (2009)
  • Author(s): 森島寿貴, 他
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋
• [Presentation] Proliferation of GABAergic interneurons but not cortical plate cells was reduced by maternal stress in the fetal cerebral cortex (2009)
  • Author(s): 内田琢, 他
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋
• [Presentation] Role of chloride transport in distinct pattern of glioblastoma cell migration (2009)
  • Author(s): 熊田竜郎, 他
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋
• [Presentation] 皮質凍結損傷で誘発したマウス微小脳回の形成期におけるGABA作動性ニューロンの集積 (2008)
  • Author(s): 王天英, 熊田竜郎, 森島寿貴, 福田敦夫
  • Organizer: 第55回中部日本生理学会
  • Place of Presentation: 長久手
• [Presentation] マウスの多小脳回モデルである凍結損傷で誘発した微小脳回における異常な皮質形成 (2008)
  • Author(s): 王天英, 熊田竜郎, 岡部明仁, 森島寿貴, 福田敦夫
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京
• [Presentation] 小脳皮質形成期の外穎粒細胞層で一時的にGABAが放出される (2008)
  • Author(s): 森島寿貴, 熊田竜郎, 古川智範, 高山千利, 吉田祥子, 福田敦夫
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京
• [Presentation] グリオーマ細胞の移動時における時空間的な[C1-]_iの変化 (2008)
  • Author(s): 熊閏竜郎, 福閏敦夫
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京
• [Presentation] 三叉神経痛モデルの三叉神経脊髄路核と神経節におけるKCC2とNKCCIの発現変化 (2008)
  • Author(s): 福田敦夫, 魏兵, 古川智範, 熊田竜郎, 佐藤康二
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京
• [Presentation] 三叉神経痛モデルの三叉神経脊髄路核と神経節におけるKCC2とNKCC1の発現変化 (2008)
  • Author(s): 福田敦夫
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京
• [Presentation] グリオーマ細胞の移動時における時空間的な[Cl^-」の変化 (2008)
  • Author(s): 熊田竜郎
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京
• [Remarks] ホームページ
  • URL: http://www2.hama-med.ac.jp/wla/physl/index-j.html