Elucidation of regulatory mechanism of AMPA-receptor's trafficking by visualization of its intracellular pool.

• Project/Area Number: 20500363
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurophysiology and muscle physiology
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: YAMAGUCHI Kazuhiko The Institute of Physical and Chemical Research, 運動学習制御研究チーム, 副チームリーダー (00191221)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: プルキンエ細胞 / シナプス可塑性 / 受容体トラフィッキング / グルタミン酸受容体 / スパイン / 長期抑圧 / 小脳 / アンパ型グルタミン酸受容体 / トランスロケーション / 樹状突起 / GFP / ウイルスベクター / レンチウイルス / セムリキ森林ウイルス

Research Abstract

Long-term depression (LTD) of synaptic efficacy in the cerebellar Purkinje cell provides an underlying mechanism for motor learning. We analyzed distribution of intracellular pool of AMPA-receptors (AMPA-Rs) by means of double-expression of Green fluorescent protein (GFP) and Red fluorescent protein (mCherry)-tagged AMPA-Rs in cultured Purkinje cell. We found that LTD-stimulation caused translocation of intracellular pool of AMPA-Rs from the spine to the shaft of dendrite, but it caused no change in shape of the spine.

Research Products

• [Journal Article] CRMP5 (Collapsin Response Mediator Protein 5) regulates dendritic development and synaptic plasticity in the cerebellar (2011)
  • Author(s): Yamashita N, Mosinger B, Roy A, Miyazaki M, Ugajin K, Nakamura F, Sasaki Y, Yamaguchi K, Kolattukudy P, Goshima Y
  • Journal Title: Purkinje cell.J.Neurosci. 31 Pages: 1773-1779
  • Peer Reviewed
• [Journal Article] CRMP5 (Collapsin Response Mediator Protein 5) regulates dendritic development and synaptic plasticity in the cerebellar Purkinje cell. (2011)
  • Author(s): Yamashita, et al.
  • Journal Title: J.Neuroscience Volume: 31 Pages: 1773-1779
  • Peer Reviewed
• [Journal Article] RNG105 deficiency impairs the dendritic localization of mRNAs for Na^+/K^+ ATPase subunit isoforms and leads to the degeneration of neuronal networks. (2010)
  • Author(s): Shiina N, Yamaguchi K, Tokunaga M
  • Journal Title: J.Neurosci 30 Pages: 12816-12830
  • Peer Reviewed
• [Journal Article] RNG105 deficiency impairs the dendritic localization of mRNAs for Na+/K+ ATPase subunit isoforms and leads to the degeneration of neuronal networks. (2010)
  • Author(s): Shiina N., et al.
  • Journal Title: J.Neuroscience Volume: 30 Pages: 12816-12830
  • Peer Reviewed
• [Journal Article] Dual involvement of G-substrate in motor learning revealed by gene deletion. (2009)
  • Author(s): Endo S., Shuto F., Le D.T., Okamoto T., Ikeda T., Suzuki M., Kawahara S., Yanagihara D., Sato Y., Yamada K., Sakamoto T., Kirino Y., Hartell N.A., Yamaguchi K., Itohara S., Naim A., Greengarg P., Nagao S., Ito M.
  • Journal Title: Proc.Natl.Acad.Sci.USA 106 Pages: 3525-3530
  • NAID: 120006399303
  • Peer Reviewed
• [Journal Article] Dual involvement of G-substrate in motor learning revealed by gene deletion (2009)
  • Author(s): Endo, S., et al
  • Journal Title: Proc.Natl.Acad.Sci.USA 106 Pages: 3525-3530
  • Peer Reviewed
• [Journal Article] Connexin43 and Bergmann glial gap junctions in cerebellar function. (2008)
  • Author(s): Tanaka M, Yamaguchi K, Tatsukawa T, Theis M, Willecke K, Itohara S
  • Journal Title: Front Neurosci. 2(2) Pages: 225-233
  • Peer Reviewed
• [Journal Article] Lack of connexin43-mediated Bergmann glial gap junctional coupling does not affect cerebellar long-term depression, motor coordination or eyeblink conditioning. (2008)
  • Author(s): Tanaka M., Yamaguchi K., Tatsukawa T., Nishioka C., Nishiyama H., Theis M., Willecke K., Itohara S.
  • Journal Title: Front.Behav.Neurosci. 2 Pages: 1-14
  • Peer Reviewed
• [Journal Article] Connexin43 and Bergmann glial gap junction in cerebellar function (2008)
  • Author(s): Tanaka, M.
  • Journal Title: Frontiers in Neuroscience 2 Pages: 225-233
  • Peer Reviewed
• [Presentation] 小脳プルキンエ細胞におけるGluR2細胞内プールの活動依存性トランスロケーションとシナプス可塑性:蛍光蛋白質2重発現法による解析 (2010)
  • Author(s): 山口和彦
  • Organizer: 生理学研究所研究会"シナプス伝達の概念志向型研究"
  • Place of Presentation: 岡崎
  • Year and Date: 2010-12-08
• [Presentation] 小脳プルキンエ細胞におけるGluR2細胞内プールの活動依存性トランスロケーションとシナプス可塑性:蛍光蛋白質2重発現法による解析 (2010)
  • Author(s): 山口和彦, 他
  • Organizer: 生理学研究所研究会"シナプス伝達の概念志向型研究"
  • Place of Presentation: 生理学研究所 岡崎
  • Year and Date: 2010-12-08
• [Presentation] Effects of LTD-Inducing Stimulation on Endocytic Rate-Constant of AMPA-Receptor Trafficking at Parallel Fiber-Purkinje Cell Synapse in Cerebellum (2010)
  • Author(s): Yamaguchi K., et al.
  • Organizer: 生理学研究所研究会"記憶学習行動の基盤としてのシナプス可塑性"
  • Place of Presentation: 生理学研究所 岡崎
  • Year and Date: 2010-12-02
• [Presentation] AMPA-receptor trafficking in cerebellar Purkinje cell : A kinetic analysis. Symposium "Neural circuit : Develop and Plasticity" (2010)
  • Author(s): 山口和彦
  • Organizer: 5^<th> Congress of FAONS and XXVIII Annual Meeting of IAN.
  • Place of Presentation: Lucknow India
  • Year and Date: 2010-11-27
• [Presentation] AMPA-receptor trafficking in cerebellar Purkinje cell : A kinetic analysis. (2010)
  • Author(s): Yamaguchi K.
  • Organizer: Symposium "Neural circuit : Develop and Plasticity" 5^<th> Congress of Federation of Asia-Oceania Neuroscience (FAONS)
  • Place of Presentation: Hotel Clarks Avadh, Lucknow, India
  • Year and Date: 2010-11-27
• [Presentation] Attenuation of cerebellar plasticity after neonatal perturbation of NMDA receptors in the vestibular nucleus (2010)
  • Author(s): Lai SK, Yamaguchi K, Yung WH, Chan YS
  • Organizer: Poster 5^<th> Congress of FAONS and XXVIII Annual Meeting of IAN.
  • Place of Presentation: Lucknow India
  • Year and Date: 2010-11-26
• [Presentation] Effects of LTD-Inducing Stimulation on Endocytic Rate-Constant of AMPA-Receptor Trafficking at Parallel Fiber-Purkinje Cell Synapse in Cerebellum (2010)
  • Author(s): Yamaguchi K, Nagao S
  • Organizer: 生理学研究所研究会"記憶学習行動の基盤としてのシナプス可塑性"
  • Place of Presentation: 岡崎
  • Year and Date: 2010-11-26
• [Presentation] Attenuation of cerebellar plasticity after neonatal perturbation of NMDA receptors in the vestibular nucleus (2010)
  • Author(s): Lai, et al.
  • Organizer: 5^<th> Congress of Federation of Asia-Oceania Neurosci (FAONS)
  • Place of Presentation: Hotel Clarks Avadh, Lucknow, India
  • Year and Date: 2010-11-26
• [Presentation] Perinatal perturbation of NMDA receptor in the vestibular nucleus attenuates cerebellar function. (2010)
  • Author(s): Lai SK, Shum DKY, Yung WH, Yamaguchi K, Chan YS
  • Organizer: The 40th annual meeting of the Society for Neuroscience
  • Place of Presentation: San Diego USA
  • Year and Date: 2010-11-17
• [Presentation] Perinatal perturbation of NMDA receptor in the vestibular nucleus attenuates cerebellar function. (2010)
  • Author(s): Lai, et al.
  • Organizer: 40th annual meeting of the Society for Neuroscience
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 2010-11-17
• [Presentation] Involvement of actin-myosin interaction in cerebellar long-term depression (2010)
  • Author(s): Tatsukawa T, Nagao S, Yamaguchi K
  • Organizer: The 40th annual meeting of the Society for Neuroscience
  • Place of Presentation: San Diego USA
  • Year and Date: 2010-11-14
• [Presentation] Involvement of actin-myosin interaction in cerebellar long-term depression (2010)
  • Author(s): Tatsukawa, et al.
  • Organizer: 40th annual meeting of the Society for Neuroscience
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 2010-11-14
• [Presentation] ケージドペプチドを用いた小脳平行線維プルキンエ細胞間シナプスにおけるAMPA受容体トラフィッキングの速度定数の計測 (2010)
  • Author(s): 山口和彦、達吉郎、永雄総一、伊藤正男
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-09-02
• [Presentation] AMPA-receptor trafficking in cerebellar Purkinje cell : A kinetic analysis using a whole-cell recording technique. (2010)
  • Author(s): 山口和彦
  • Organizer: IBRO School of Neuroscience Chinese University of Hong Kong
  • Place of Presentation: Hong Kong
  • Year and Date: 2010-06-02
• [Presentation] AMPA-receptor trafficking in cerebellar Purkinje cell : A kinetic analysis using a whole-cell recording technique. (2010)
  • Author(s): Yamaguchi K.
  • Organizer: IBRO School of Neuroscience in Hong Kong
  • Place of Presentation: Chinese University of Hong Kong, Hong Kong
  • Year and Date: 2010-06-02
• [Presentation] 小脳プルキンエ細胞GluR2細胞内プールの樹状突起スパインからシャフトへの活動依存性移動の蛍光蛋白質2重発現法による解析 (2010)
  • Author(s): 山口和彦、清水知佳、中澤敬信、伊藤正男
  • Organizer: 第87回日本生理学会大会
  • Place of Presentation: 盛岡
  • Year and Date: 2010-05-19
• [Presentation] Translocation of GluR2 pool in the dendritic spine of cerebellar Purkinje cells under long-term depression. (2009)
  • Author(s): Yamaguchi K, Shimizu C, Sato Y, Furuichi T, Ito M
  • Organizer: The 39^<th> annual meeting of the Society for Neuroscience
  • Place of Presentation: Chicago USA
  • Year and Date: 2009-10-19
• [Presentation] Translocation of GluR2 pool in the dendritic spine of cerebellar Purkinje cells under long-term depression (2009)
  • Author(s): Yamaguchi K, Shimizu C, Sato Y, Furuichi T, Ito M.
  • Organizer: 39^<th> annual meeting of Society for Neuroscience
  • Place of Presentation: Chicago IL USA
  • Year and Date: 2009-10-19
• [Presentation] プルキンエ細胞樹状突起スパインにおけるGluR2細胞内プールの活動依存性移動へのPKCの関与 (2009)
  • Author(s): 山口和彦
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-09-16
• [Presentation] プルキンエ細胞樹状突起スパインにおけるGluR2細胞内プールの活動依存性移動へのPKCの関与 (2009)
  • Author(s): 山口和彦
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: (名古屋国際会議場)名古屋
  • Year and Date: 2009-09-16
• [Presentation] An integrative kinetic model of AMPA-receptors constitutive trafficking and LTD/LTP in cerebellar (2009)
  • Author(s): 山口和彦
  • Organizer: Purkinje cell 36^<th> International Congress of Physiological Sciences
  • Place of Presentation: 京都
  • Year and Date: 2009-07-28
• [Presentation] An integrative kinetic model of AMPA-receptors constitutive trafficking and LTD/LTP in cerebellar Purkinje cell (2009)
  • Author(s): 山口和彦
  • Organizer: 36^<th> International Congress of Physiological Sciences
  • Place of Presentation: (国立京都国際会館)京都
  • Year and Date: 2009-07-28
• [Presentation] AMPA受容体トラフィッキングのキネティックスとシナプス可塑性 (2009)
  • Author(s): 山口和彦
  • Organizer: 神経組織の成長・再生・移植研究会題24回学術集会
  • Place of Presentation: 伊香保
  • Year and Date: 2009-06-21
• [Presentation] AMPA受容体トラフィッキングのキネティックスとシナプス可塑性 (2009)
  • Author(s): 山口和彦
  • Organizer: 神経組織の成長・再生・移植研究会第24回学術集会
  • Place of Presentation: (ホテル天坊)群馬県伊香保
  • Year and Date: 2009-06-21
• [Presentation] RNG105 deficiency impairs the denderitic transport on Na+/K+-ATPase subunit isoform mRNA and the formation of synapses and neuronal networks. (2008)
  • Author(s): Shiina N, Yamaguchi K, Tokunaga M
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会 合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-11
• [Presentation] RNG105 deficiency impairs the dendritic transport of Na +/K+ ATPase subunit isoform mRNAs and the formation of synapses and neuronal networks (2008)
  • Author(s): Shiina, Nobuyuki
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2008-12-11
• [Presentation] LRR膜貫通分子Lrfn2/SALM1は海馬興奮性シナプスの形態や機能調節および可塑性に関与する (2008)
  • Author(s): 守村直子、山口和彦、安田浩樹、片山圭一、原直子、太田摩耶、神谷明子、有賀純
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会 合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] LRR膜貫通分子Lrfn2/SALM1は海馬興奮性シナプスの形態や機能調節および可塑性に関与する (2008)
  • Author(s): 守村直子
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2008-12-10
• [Presentation] 小脳プルキンエ細胞におけるAMPA受容体トラフィッキングの速度論的解析とLTP/LTDのメカニズム (2008)
  • Author(s): 山口和彦
  • Organizer: 生理学研究所研究会 シナプス研究会"シナプス成熟と可塑性のダイナミクス"
  • Place of Presentation: 岡崎
  • Year and Date: 2008-12-04
• [Presentation] プルキンエ細胞におけるAMPA受容体双方向性トラフィッキングとモデルによる考察 (2008)
  • Author(s): 山口和彦
  • Organizer: 生理学研究所研究会"新たなコンセプトでシナプス伝達を考える"
  • Place of Presentation: 岡崎
  • Year and Date: 2008-09-18
• [Presentation] アラキドン酸はアストロサイトの接触によって誘発されるシナプス形成のカギをにぎる (2008)
  • Author(s): 濱裕、安藤亮子、山口和彦、宮脇敦史
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京
  • Year and Date: 2008-07-11
• [Presentation] アラキドン酸はアストロサイトの接触によって誘発されるシナプス形成のカギをにぎる (2008)
  • Author(s): 濱裕
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京国際フォーラム
  • Year and Date: 2008-07-11
• [Presentation] 小脳プルキンエ細胞樹状突起スパインにおけるアンパ受容体分布変化と長期抑圧 (2008)
  • Author(s): 山口和彦、清水知佳、佐藤友美、古市貞一、伊藤正男
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京
  • Year and Date: 2008-07-10
• [Presentation] 小脳プルキンエ細胞樹状突起スパインにおけるAMPA受容体分布変化と長期抑圧 (2008)
  • Author(s): 山口和彦
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京国際フォーラム
  • Year and Date: 2008-07-10
• [Presentation] 小脳プルキンエ細胞樹状突起におけるグルタミン酸受容体の活動依存性分布変化 (2008)
  • Author(s): 山口和彦、清水知佳、佐藤友美、古市貞一、伊藤正男
  • Organizer: 第85回日本生理学会大会
  • Place of Presentation: 東京
  • Year and Date: 2008-03-26
• [Presentation] Roles of actin in constitutive and activity-dependent trafficking of AMPA-receptors in the cerebellar Purkinje cell. (2008)
  • Author(s): 山口和彦
  • Organizer: US-Japan Brain Research Collaborative Program. Workshop on Receptor Trafficking and Cell Biology of Neurons : Physiology and Disease.
  • Place of Presentation: Pacific Grove USA
  • Year and Date: 2008-02-26
• [Book] 分子・細胞・シナプス (2008)
  • Author(s): 山口和彦
  • Publisher: 東京大学出版会