Elucidation of regulatory mechanism of AMPA-receptor's trafficking by visualization of its intracellular pool.
| Project/Area Number |
20500363
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurophysiology and muscle physiology
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
YAMAGUCHI Kazuhiko The Institute of Physical and Chemical Research, 運動学習制御研究チーム, 副チームリーダー (00191221)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | プルキンエ細胞 / シナプス可塑性 / 受容体トラフィッキング / グルタミン酸受容体 / スパイン / 長期抑圧 / 小脳 / アンパ型グルタミン酸受容体 / トランスロケーション / 樹状突起 / GFP / ウイルスベクター / レンチウイルス / セムリキ森林ウイルス |
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| Research Abstract |
Long-term depression (LTD) of synaptic efficacy in the cerebellar Purkinje cell provides an underlying mechanism for motor learning. We analyzed distribution of intracellular pool of AMPA-receptors (AMPA-Rs) by means of double-expression of Green fluorescent protein (GFP) and Red fluorescent protein (mCherry)-tagged AMPA-Rs in cultured Purkinje cell. We found that LTD-stimulation caused translocation of intracellular pool of AMPA-Rs from the spine to the shaft of dendrite, but it caused no change in shape of the spine.
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Report
(4 results)
Research Products
(46 results)
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[Journal Article] Dual involvement of G-substrate in motor learning revealed by gene deletion.2009
Author(s)
Endo S., Shuto F., Le D.T., Okamoto T., Ikeda T., Suzuki M., Kawahara S., Yanagihara D., Sato Y., Yamada K., Sakamoto T., Kirino Y., Hartell N.A., Yamaguchi K., Itohara S., Naim A., Greengarg P., Nagao S., Ito M.
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Journal Title
Proc.Natl.Acad.Sci.USA 106
Pages: 3525-3530
NAID
Related Report
Peer Reviewed
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