Validation system of chemicals using BAC vector transfected ES cells

• Project/Area Number: 20510063
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Ritsumeikan University
• Principal Investigator: TAKADA Tatsuyuki Ritsumeikan University, 薬学部, 教授 (90206756)
• Co-Investigator(Kenkyū-buntansha): OKAMOTO Yoshinori 名城大学, 薬学部, 助教 (50512323) ; NOCE Toshiaki 滋賀医科大学, 動物生命科学研究センター, 特任教授 (70183902)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 内分泌撹乱物質 / ES細胞 / 細胞分化 / 生殖細胞分化 / 生殖細胞遺伝子発現 / Bacベクター / 環境分析

Research Abstract

Germ cell related gene which expression was up-regulated by the exposure to bisphenol A(BPA) was identified. ES cell line which express venus under the control of this promoter was established. This ES cell line express venus in the presence of chemicals in a dose-dependent manner, suggesting validation system of environmental chemicals can be achieved using reporter ES cells.

Research Products

• [Journal Article] oligoselenodiglutathiones and their suppressive effects on oxidative DNA damage induced by catechol and copper (2011)
  • Author(s): Ando M., Nishida H., Ueda K., Itoh K., Okamoto Y., Kojima N
  • Journal Title: J.Health Sci. 57 Pages: 72-77
  • Peer Reviewed
• [Journal Article] Anti-breast cancer potential of SS5020, a novel benzopyran antiestrogen (2011)
  • Author(s): Suzuki N., Liu X., Laxmi Y.R.S.Okamoto K., Kim H.J., Zhang G., Chen J.J., Okamoto Y., Shibutani S.
  • Journal Title: Int.J.Cancer 128 Pages: 974-982
  • Peer Reviewed
• [Journal Article] Anti-breast cancer potential of SS5020, a novel benzopyran antiestrogen (2011)
  • Author(s): Suzuki N., Liu X., Laxmi Y.R.S., Okamoto K., Kim H.J., Zhang G., Chen J.J., Okamoto Y., Shibutani S.
  • Journal Title: Int.J.Cancer 128, 974-982 Volume: 128 Pages: 974-982
  • Peer Reviewed
• [Journal Article] oligoselenodiglutathiones and their suppressive effects on oxidative DNA damage induced by catechol and copper (2011)
  • Author(s): Ando M., Nishida H., Ueda K., Itoh K., Okamoto Y., Kojima N.
  • Journal Title: J.Health Sci.57, 72-77 Volume: 57 Pages: 72-77
  • Peer Reviewed
• [Journal Article] Different mechanisms between copper and iron in catecholamines-mediated oxidative DNA damage and disruption of gene expression in vitro. (2011)
  • Author(s): Nishino Y., Ando M., Makino R.,Ueda K., Okamoto Y., Kojima N.
  • Journal Title: Neurotox.Res. Volume: (in press(掲載確定))
  • Peer Reviewed
• [Journal Article] Combined effects of manganese, iron, copper, and dopamine on oxidative DNA damage (2011)
  • Author(s): Ando M., Ueda K., Okamoto Y., Kojima N.
  • Journal Title: J.Health Sci. Volume: (in press(掲載確定))
  • NAID: 130000663122
  • Peer Reviewed
• [Journal Article] Transesterification in the microbial degradation of phthalate esters. (2011)
  • Author(s): Okamoto Y., Toda C., Ueda K., Hashizume K., Kojima N.
  • Journal Title: J.Health Sci. Volume: (in press(掲載確定))
  • NAID: 130000800091
  • Peer Reviewed
• [Journal Article] Carbonyl side-chain of catechol compounds is a key structure for the suppression of copper-associated oxidative DNA damage in vitro (2010)
  • Author(s): Ando M., Nishida H., Nishino Y., Ohbayashi M., Ueda K., Okamoto Y., Kojima N.
  • Journal Title: Toxicol.Lett. 199 Pages: 213-217
  • Peer Reviewed
• [Journal Article] Production of polyselenodipenicillamines, unique selenium compounds (2010)
  • Author(s): Nishida H., Ando M., Itoh K., Ueda K., Nishida Y., Okamoto Y., Toda C., Kojima N.
  • Journal Title: Chem.Pharm.Bull. 58 Pages: 957-960
  • NAID: 130000299793
  • Peer Reviewed
• [Journal Article] Anti-breast cancer potential of SS1020, a novel antiestrogen lacking estrogenic and genotoxic actions (2010)
  • Author(s): Laxmi YR, Liu X, Suzuki N, Kim SY, Okamoto K, Kim HJ, Zhang G, Chen JJ, Okamoto Y, Shibutani S.
  • Journal Title: Int J Cancer 127 Pages: 1718-26
  • Peer Reviewed
• [Journal Article] Equine estrogen-induced mammary tumors in rats (2010)
  • Author(s): Okamoto Y, Liu X, Suzuki N, Okamoto K, Kim HJ, Laxmi YR, Sayama K, Shibutani S.
  • Journal Title: Toxicol Lett. 193 Pages: 224-8
  • Peer Reviewed
• [Journal Article] Production of polyselenodipenicillamines, unique selenium compounds (2010)
  • Author(s): Nishida H., Ando M., Itoh K., Ueda K., Nishida Y., Okamoto Y., Toda C., Kojima N.
  • Journal Title: Chem.Pharm.Bull. Volume: 58 Pages: 957-960
  • NAID: 130000299793
  • Peer Reviewed
• [Journal Article] Carbonyl side-chain of catechol compounds is a key structure for the suppression of copper-associated oxidative DNA damage in vitro (2010)
  • Author(s): Ando M., Nishida H., Nishino Y., Ohbayashi M., Ueda K., Okamoto Y., Kojima N.
  • Journal Title: Toxicol.Lett. Volume: 199 Pages: 213-217
  • Peer Reviewed
• [Journal Article] Anti- breast cancer potential of SS1020, a novel antiestrogen lacking estrogenic and genotoxic actions (2010)
  • Author(s): Laxmi YR, Liu X, Suzuki N, Kim SY, Okamoto K, Kim HJ, Zhang G, Chen JJ, Okamoto Y, Shibutani S.
  • Journal Title: Int J Cancer (In press(掲載決定))
  • Peer Reviewed
• [Journal Article] Membrane localization of protein-tyrosine phosphatase 1B is essential for its activation of sterol regulatory element-binding protein-1 gene expression and consequent hypertriglyceridaemia. (2009)
  • Author(s): Ugi, S., Shi, K., Nishio, Y., Shimizu, S., Guo, B., Sekine, O., Ikeda, K., Egawa, K., Yoshizaki, T., Nagai, Y., Koya, D., Takada, T., Torii, R., Kimura, H., Kashiwagi, A., Maegawa, H.
  • Journal Title: J.Biochem. 146 Pages: 541-547
  • NAID: 10026304029
  • Peer Reviewed
• [Journal Article] Increased antitumor potential of the raloxifene prodrug, raloxifene diphosphate (2008)
  • Author(s): Okamoto Y, Liu X, Suzuki N, Okamoto K, Sekimoto M, Laxmi Y.R.S., Shibutani S
  • Journal Title: Int.J.Cancer 112 Pages: 2142-2147
  • Peer Reviewed
• [Journal Article] Oxidative DNA damage in XPC-knockout and its wild mice treated with equine estrogen. (2008)
  • Author(s): Okamoto Y. Chou P.H, Kim S.Y., Suzuki N, Laxmi Y. R. S., Okamoto K, Liu X, Matsuda T, Shibutani S
  • Journal Title: Chem.Res.Toxicol 21 Pages: 1120-1124
  • Peer Reviewed
• [Journal Article] Combined activation of methyl paraben by light irradiation and esterase metabolism toward oxidative DNA damage (2008)
  • Author(s): Okamoto Y, T. Hayashi T, Matsunami S, Ueda K, Kojima N.
  • Journal Title: Chem.Res.Toxicol. 21 Pages: 1594-1599
  • Peer Reviewed
• [Journal Article] BMP4 induces primitive endoderm but not trophectoderm in monkey embryonic stem cells (2008)
  • Author(s): Kobayashi M., Takada T., Takahashi K., Noda Y., Torii R.
  • Journal Title: Cloning Stem Cells 10 Pages: 495-502
  • Peer Reviewed
• [Journal Article] Differences and similarities in the developmental status of embryo-derived stem cells and primordial germ cells revealed by global expression profiling. (2008)
  • Author(s): Mise N, Fuchikami T, Sugimoto M, Kobayakawa S, Ike F, Ogawa T, Tada T, Kanaya S, Noce T, Abe K.
  • Journal Title: Genes Cells 13 Pages: 67-80
  • Peer Reviewed
• [Journal Article] Identification of transcripts commonly expressed in both hematopoietic and germ-line stem cells (2008)
  • Author(s): Mizukami T, Kuramitsu M, Takizawa K, Momose H, Masumi A, Naito S, Iwama A, Ogawa T, Noce T, Hamaguchi I, Yamaguchi K
  • Journal Title: Stem Cells Dev. 17 Pages: 67-80
  • Peer Reviewed
• [Journal Article] Combined activation of methyl paraben by light irradiation and esterase metabolism toward oxidative DNA damage (2008)
  • Author(s): Okamoto Y, T. Hayashi T, Matsunami S, Ueda K, Kojima N.
  • Journal Title: Chem. Res. Toxicol. 21 Pages: 1594-1599
  • Peer Reviewed
• [Journal Article] Oxidative DNA damage in XPC-knockout and its wild mice treated with equine estrogen. (2008)
  • Author(s): Okamoto Y. Chou P. H, Kim S. Y., Suzuki N, Laxmi Y. R. S., Okamoto K, Liu X, Matsuda T, Shibutani S
  • Journal Title: Chera. Res. Toxicol 21 Pages: 1120-1124
  • Peer Reviewed
• [Journal Article] Increased antitumor potential of the raloxifene prodrug, raloxifene diphosphate (2008)
  • Author(s): Okamoto Y, Liu X, Suzuki N, Okamoto K, Sekimoto M, Laxmi Y. R. S., Shibutani S
  • Journal Title: Int. J. Cancer 112 Pages: 2142-2147
  • Peer Reviewed
• [Presentation] Expression of post-meiotic markers during differentiation of non-human primate ES cells in vitro (2011)
  • Author(s): Takada T., Shimozawa N., Noce T., Suemori H., Ohta Y.
  • Organizer: 9th Annual Meeting of the International Society for Stem Cell Research
  • Place of Presentation: Tronto, Canada
  • Year and Date: 2011-06-17
• [Presentation] Expression of post-meiotic markers during differentiation of non-human primate ES cells in vitro (2011)
  • Author(s): Takada T., Shimozawa N., Noce T., Suemori H., Ohta Y.
  • Organizer: 9th Annual Meeting of the International Society for Stem Cell Research
  • Place of Presentation: Tront, Canada
  • Year and Date: 2011-06-17
• [Presentation] Bisphenol AがES細胞の生殖細胞分化に与える影響 (2011)
  • Author(s): 青木智弘、高田達之
  • Organizer: 第38回日本トキシコロジー学会学術年会
  • Place of Presentation: パシフィコ横浜(神奈川県)(発表確定・発表日未定)
• [Presentation] Bisphenol AがES細胞の生殖細胞分化に与える影響 (2011)
  • Author(s): 青木智弘、高田達之
  • Organizer: 第38回日本トキシコロジー学会学術年会
  • Place of Presentation: パシフィコ横浜(神奈川)(発表確定)
• [Presentation] マウスES細胞の生殖細胞分化におけるBisphenolAの影響 (2010)
  • Author(s): 青木智弘、高田達之
  • Organizer: 第13回日本内分泌撹乱化学物質学会
  • Place of Presentation: 東京大学(東京都)
  • Year and Date: 2010-12-17
• [Presentation] マウスES細胞の生殖細胞分化におけるBisphenolAの影響 (2010)
  • Author(s): 青木智弘、高田達之
  • Organizer: 第13回日本内分泌撹乱化学物質学会
  • Place of Presentation: 東京大学(東京)
  • Year and Date: 2010-12-17
• [Presentation] カニクイザルES細胞における生殖細胞分化 (2010)
  • Author(s): 太田康夫、下澤律浩、末盛博文、野瀬俊明、高田達之
  • Organizer: 第33回日本分子生物学会、第83 回日本生化学会、合同大会
  • Place of Presentation: 神戸国際展示場(兵庫県)
  • Year and Date: 2010-12-09
• [Presentation] カニクイザルES細胞における生殖細胞分化 (2010)
  • Author(s): 太田康夫、下澤律浩、末盛博文、野瀬俊明、高田達之
  • Organizer: 第33回日本分子生物学会、第83回日本生化学会
  • Place of Presentation: 神戸国際展示場(兵庫)
  • Year and Date: 2010-12-09
• [Presentation] Expression of post-meiotic markers during differentiation of non-human primate ES cells in vitro (2010)
  • Author(s): Ohta Y., Shimozawa N., Noce T., Suemori H., Takada T.
  • Organizer: International Symposium on Epigenome Network, Development and Reprogramming of Germ Cells
  • Place of Presentation: 九州大学(福岡県)
  • Year and Date: 2010-11-23
• [Presentation] Expression of post-meiotic markers during differentiation of non-human primate ES cells in vitro (2010)
  • Author(s): Ohta Y., Shimozawa N., Noce T., Suemori H., Takada T.
  • Organizer: International Symposium on Epigenome Network Development and Reprogramming of Germ Cells
  • Place of Presentation: Kyusyu Univ.(福岡)
  • Year and Date: 2010-11-23
• [Presentation] Non-human primate ES細胞の生殖細胞分化 (2010)
  • Author(s): 太田康夫、下澤律浩、野瀬俊明、高田達之
  • Organizer: 文部科学省・科学研究費補助金・特定領域研究「生殖系列の世代サイクルとエピゲノムネットワーク」生殖サイクル若手勉強会2010
  • Place of Presentation: つくばグランドホテル(茨城)
  • Year and Date: 2010-07-22
• [Presentation] Non-human primate ES細胞の分化過程における生殖細胞マーカー遺伝子の発現変化 (2009)
  • Author(s): 太田康夫、橋本圭史、青木智弘、岡本誉士典、鳥居隆三、野瀬俊明、高田達之
  • Organizer: 文部科学省・科学研究費補助金・特定領域研究「生殖系列の世代サイクルとエピゲノムネットワーク」
  • Place of Presentation: 第2回公開シンポジウム、コクヨホール(東京都)
  • Year and Date: 2009-11-27
• [Presentation] Non-human primate ES 細胞の生殖細胞への分化 (2009)
  • Author(s): 太田康夫、橋本圭史、青木智弘、岡本誉士典、鳥居隆三、野瀬俊明、高田達之
  • Organizer: 文部科学省・科学研究費補助金・特定領域研究「生殖系列の世代サイクルとエピゲノムネットワーク」生殖サイクル若手勉強会2009
  • Place of Presentation: 御殿場高原ホテル(静岡県)
  • Year and Date: 2009-08-28
• [Presentation] Derivation of rat cell line which contribute to visceral yolk sac (2009)
  • Author(s): Takada T., Masuda C., Okamoto Y., Tooyama I., Kato M., Hirabayashi M.
  • Organizer: 7th Annual Meeting of the International Society for Stem Cell Research
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2009-07-10
• [Presentation] BMP4はカニクイザルES 細胞をprimitive endodermへ分化させる (2008)
  • Author(s): 小林昌、高田達之、高橋健太郎、野田洋一、鳥居隆三
  • Organizer: 第31回日本分子生物学会、第81回日本生化学会、合同大会
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
  • Year and Date: 2008-12-10
• [Presentation] BMP4はカニクイザルES細胞をprimitive endodermへ分化させる (2008)
  • Author(s): 高田達之
  • Organizer: 第31回日本分子生物学会、第81回日本生化学会、合同大会
  • Place of Presentation: 神戸国際会議場(兵庫県)
  • Year and Date: 2008-12-09
• [Remarks] ホームページ等
  • URL: http://www.collabo.sk.ritsumei.ac.jp/laboratory/takada.htm
• [Remarks]
  • URL: http://www.collabo.sk.ritsumei.ac.jp/laboratory/takada.htm
• [Patent(Industrial Property Rights)] 名遺伝子中のメチル化塩基の迅速・超高感度分析法 (2011)
  • Inventor(s): 高田達之、小嶋仲夫、岡本誉士典
  • Industrial Property Rights Holder: 立命館大学、名城大学
  • Industrial Property Number: 2011-102765
  • Filing Date: 2011-05-02