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Spacio-temporal analysis of histone modification by FRET probes

Research Project

Project/Area Number 20510183
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 基礎ゲノム科学
Research InstitutionTokyo Medical and Dental University

Principal Investigator

TANAKA Yuujirou  Tokyo Medical and Dental University, 大学院・疾患生命科学研究部, 准教授 (00311613)

Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsクロマチン構造 / ヒストン修飾 / メチル基転移酵素 / 脱メチル化酵素 / ヒストンメチル基転移酵素 / FRET / リジルエンドペプチダーゼ / 酵素活性 / FRETプローブ / 可視化
Research Abstract

A new technique of visualization of histone modifying enzymes was established using FRET peptides and artificially engineered lysyl endopeptidases. The method was successfully employed to visualize intra-cellular histone modifying enzyme activity.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (12 results)

All 2010 2009 2008 Other

All Journal Article (5 results) (of which Peer Reviewed: 4 results) Presentation (5 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Development of novel bisubstrate-type inhibitors of histone methyltransferase SET7/9.2010

    • Author(s)
      Mori S, Iwase K, Iwanami N, Tanaka Y, Kagechika H, Hirano T.
    • Journal Title

      Bioorg Med Chem. 18

      Pages: 8158-8166

    • Related Report
      2010 Final Research Report
  • [Journal Article] Development of novel bisubstrate-type inhibitors of histone methyltransferase SET7/92010

    • Author(s)
      Mori S, Iwase K, Iwanami N, Tanaka Y, Kagechika H, Hirano T.
    • Journal Title

      Bioorg Med Chem

      Volume: 18 Pages: 8158-8166

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Differential usage of alternate promoters of the human stress response gene ATF3 in stress response and cancer cells.2009

    • Author(s)
      Miyazaki K
    • Journal Title

      Nucleic Acids Res. 37

      Pages: 1438-51

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Regulation of early T cell development by PHD finger of histone lysine methyltransferase ASH12008

    • Author(s)
      Tanaka Y, Nakayama Y, Tani guchi M, Kioussis D
    • Journal Title

      Biochem Biophys Res Commun 365

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Differential usage of alternate promoters of the human stress response gene ATF3 in stress response and cancer cells2008

    • Author(s)
      Miyazaki K, et al.
    • Journal Title

      Nucleic Acids Res 37

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation]2010

    • Organizer
      定量生物学の会第3回年会
    • Place of Presentation
      東京大学生産技術研究所
    • Year and Date
      2010-11-26
    • Related Report
      2010 Final Research Report
  • [Presentation] 細胞内ヒストン修飾酵素活性の測定2010

    • Author(s)
      田中裕二郎
    • Organizer
      定量生物学の会第3回年会
    • Place of Presentation
      東京大学生産技術研究所(東京都)
    • Related Report
      2010 Annual Research Report
  • [Presentation] システムズ・バイオテクノロジーによるATF3標的遺伝子の網羅的探索2009

    • Author(s)
      中村絢
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2009-12-12
    • Related Report
      2009 Annual Research Report
  • [Presentation] ATF3による転写抑制のメカニズム2009

    • Author(s)
      劉芹
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2009-12-10
    • Related Report
      2009 Annual Research Report
  • [Presentation] ヒストンメチル基転移酵素ASHlによるHox遺伝子発現制御2008

    • Author(s)
      Tanaka Y
    • Organizer
      日本分子生物学会・日本生化学学会年会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2008-12-10
    • Related Report
      2008 Annual Research Report
  • [Remarks] ホームページ等

    • URL

      http://www.tmd.ac.jp/mri/bgen/open.html

    • Related Report
      2010 Final Research Report
  • [Patent(Industrial Property Rights)] ヒストン修飾酵素活性測定法

    • Inventor(s)
      田中裕二郎
    • Industrial Property Rights Holder
      田中裕二郎
    • Related Report
      2010 Final Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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