Spacio-temporal analysis of histone modification by FRET probes

• Project/Area Number: 20510183
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 基礎ゲノム科学
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: TANAKA Yuujirou Tokyo Medical and Dental University, 大学院・疾患生命科学研究部, 准教授 (00311613)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: クロマチン構造 / ヒストン修飾 / メチル基転移酵素 / 脱メチル化酵素 / ヒストンメチル基転移酵素 / FRET / リジルエンドペプチダーゼ / 酵素活性 / FRETプローブ / 可視化

Research Abstract

A new technique of visualization of histone modifying enzymes was established using FRET peptides and artificially engineered lysyl endopeptidases. The method was successfully employed to visualize intra-cellular histone modifying enzyme activity.

Research Products

• [Journal Article] Development of novel bisubstrate-type inhibitors of histone methyltransferase SET7/9. (2010)
  • Author(s): Mori S, Iwase K, Iwanami N, Tanaka Y, Kagechika H, Hirano T.
  • Journal Title: Bioorg Med Chem. 18 Pages: 8158-8166
• [Journal Article] Development of novel bisubstrate-type inhibitors of histone methyltransferase SET7/9 (2010)
  • Author(s): Mori S, Iwase K, Iwanami N, Tanaka Y, Kagechika H, Hirano T.
  • Journal Title: Bioorg Med Chem Volume: 18 Pages: 8158-8166
  • Peer Reviewed
• [Journal Article] Differential usage of alternate promoters of the human stress response gene ATF3 in stress response and cancer cells. (2009)
  • Author(s): Miyazaki K
  • Journal Title: Nucleic Acids Res. 37 Pages: 1438-51
  • Peer Reviewed
• [Journal Article] Regulation of early T cell development by PHD finger of histone lysine methyltransferase ASH1 (2008)
  • Author(s): Tanaka Y, Nakayama Y, Tani guchi M, Kioussis D
  • Journal Title: Biochem Biophys Res Commun 365
  • Peer Reviewed
• [Journal Article] Differential usage of alternate promoters of the human stress response gene ATF3 in stress response and cancer cells (2008)
  • Author(s): Miyazaki K, et al.
  • Journal Title: Nucleic Acids Res 37
  • Peer Reviewed
• [Presentation] (2010)
  • Organizer: 定量生物学の会第3回年会
  • Place of Presentation: 東京大学生産技術研究所
  • Year and Date: 2010-11-26
• [Presentation] 細胞内ヒストン修飾酵素活性の測定 (2010)
  • Author(s): 田中裕二郎
  • Organizer: 定量生物学の会第3回年会
  • Place of Presentation: 東京大学生産技術研究所(東京都)
• [Presentation] システムズ・バイオテクノロジーによるATF3標的遺伝子の網羅的探索 (2009)
  • Author(s): 中村絢
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-12
• [Presentation] ATF3による転写抑制のメカニズム (2009)
  • Author(s): 劉芹
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-10
• [Presentation] ヒストンメチル基転移酵素ASHlによるHox遺伝子発現制御 (2008)
  • Author(s): Tanaka Y
  • Organizer: 日本分子生物学会・日本生化学学会年会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2008-12-10
• [Remarks] ホームページ等
  • URL: http://www.tmd.ac.jp/mri/bgen/open.html
• [Patent(Industrial Property Rights)] ヒストン修飾酵素活性測定法
  • Inventor(s): 田中裕二郎
  • Industrial Property Rights Holder: 田中裕二郎