Proteomic analysis of oxidative stress-responsive proteins in metabolic syndrome

• Project/Area Number: 20510192
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied genomics
• Research Institution: Osaka Medical College
• Principal Investigator: SAKAI Akiko Osaka Medical College, 医学部, 助教 (30225750)
• Co-Investigator(Kenkyū-buntansha): HAYASHI Tetsuya 大阪医科大学, 医学部, 准教授 (30257852) ; YOSHIDA Hideji 大阪医科大学, 医学部, 准教授 (60288735)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 低酸素ストレス / プロテオミクス / 等電点二次元電気泳動法 / Replica blotting / MALDI / 等電点電気泳動法

Research Abstract

To determine the effects of oxidative stress on metabolic syndrome in vivo, a proteomic analysis was performed in diabetes and control mouse using two-dimensional electrophoresis and MALDI-TOF-TOF-MS. The results showed that differentially expressed proteins with important functions in cellular response to hypoxia were identified compared with normoxia condition. These findings provided new insights into the molecular mechanisms in mediating cellular response to hypoxia.

Research Products

• [Journal Article] Phosphoproteome Profiling Using a Fluorescent Phosphosensor Dye in Two-Dimensional Polyacrylamide Gel Electrophoresis. (2011)
  • Author(s): Otani M, Taniguchi T, Sakai A, Seta J, Kadoyama K, Nakamura-Hirota T, Matsuyama S, Sano K, Takano M
  • Journal Title: Appl.Biochem.Biotechnol (Epub ahead of print)
  • Peer Reviewed
• [Journal Article] Comparative proteomic analysis of the ribosomes in 5-fluorouracil resistance of a human colon cancer cell line using the radical-free and highly reducing method of two-dimensional polyacrylamide gel electrophoresis. (2010)
  • Author(s): Kimura K, Wada A, Ueta M, Ogata A, Tanaka S, Sakai A, Yoshida H, Fushitani H, Miyamoto A, Fukushima M, Uchiumi T, Tanigawa N
  • Journal Title: Int.J.Oncol 37 Pages: 1271-1278
  • Peer Reviewed
• [Journal Article] Peroxisome proliferation activation receptor alpha modulation of Ca2+-regulated exocytosis via arachidonic acid in guinea-pig antral mucous cells. (2010)
  • Author(s): Sawabe Y, Shimamoto C, Sakai A, Kuwabara H, Saad AH, Nakano T, Takitani K, Tamai H, Mori H, Marunaka Y, Nakahari T
  • Journal Title: Exp Physiol 95 Pages: 858-868
  • Peer Reviewed
• [Journal Article] Impact of the renin-angiotensin-aldosterone-system on cardiovascular and renal complications in diabetes mellitus. (2010)
  • Author(s): Hayashi T, Takai S, Yamashita C
  • Journal Title: Curr.Vasc.Pharmacol 8 Pages: 189-197
  • Peer Reviewed
• [Journal Article] 薬剤耐性を獲得した大腸癌細胞株におけるプロテオーム解析 (2010)
  • Author(s): 境晶子
  • Journal Title: 大阪医科大学雑誌 69 Pages: 39-40
• [Journal Article] Comparative proteomic analysis of the ribosomes in 5-fluorouracil resistance of a human colon cancer cell line using the radical-free and highly reducing method of two-dimensional polyacrylamide gel electrophoresis. (2010)
  • Author(s): Kimura K., et al
  • Journal Title: Int.J.Oncol Volume: 37 Pages: 1271-1278
  • Peer Reviewed
• [Journal Article] Phosphoproteome Profiling Using a Fluorescent Phosphosensor Dye in Two-Dimensional Polyacrylamide Gel Electrophoresis. (2010)
  • Author(s): Otani M., et al
  • Journal Title: Appl.Biochem.Biotechnol Volume: Mar 8(Epub ahead of print)
  • Peer Reviewed
• [Journal Article] Impact of the renin-angiotensin-aldosterone-system on cardiovascular and renal complications in diabetes mellitus. (2010)
  • Author(s): Hayashi T., et al
  • Journal Title: Curr Vasc Pharmacol Volume: 8 Pages: 189-197
  • Peer Reviewed
• [Journal Article] Use of a phosphosensor dye in proteomic analysis of human mutant tautransgenic mice. (2009)
  • Author(s): Takano M, Otani M, Sakai A, Kadoyama K, Matsuyama S, Matsumoto A, Takenokuchi M, Sumida M, Taniguchi T
  • Journal Title: Molecular neuroscience 20 Pages: 1648-1653
  • Peer Reviewed
• [Journal Article] Activities of Escherichia coliribosomes in IF3 and RMF change to prepare 100S ribosome formation on entering the stationary growth phase. (2009)
  • Author(s): Yoshida H, Ueta M, Maki Y, Sakai A, Wada A
  • Journal Title: Genes to Cells 14 Pages: 271-280
  • Peer Reviewed
• [Journal Article] Chymase plays an important role in left ventricular remodeling induced by intermittent hypoxia in mice. (2009)
  • Author(s): Matsumoto C, Hayashi T, Kitada K, Yamashita C, Miyamura M, Mori T, Ukimura A, Ohkita M, Jin D, Takai S, Miyazaki M, Okada Y, Kitaura Y, Matsumura Y
  • Journal Title: Hypertension 54 Pages: 164-171
  • Peer Reviewed
• [Journal Article] Use of a phosphosensor dye in proteomic analysis of human mutanttau transgenic mice (2009)
  • Author(s): Takano, M.
  • Journal Title: Molecular neuroscience 20 Pages: 1648-1653
  • Peer Reviewed
• [Journal Article] Activities of Escherichia coil ribosomes in IF3 and RMF change to prepare 100S ribosome formation on entering the stationary growth phase (2009)
  • Author(s): Yoshida, H.
  • Journal Title: Genes to Cells 14 Pages: 271-280
  • Peer Reviewed
• [Journal Article] Chymase plays an important role in left ventricular remodeling induced by intermittent hypoxia in mice (2009)
  • Author(s): Matumoto, C.
  • Journal Title: Hypertension 54 Pages: 164-171
  • Peer Reviewed
• [Journal Article] Proteomic analysis of the basic proteins in 5-fluorouracil resistance of human colon cancer cell line using the radical-free and highly reducing method of two-dimensional polyacrylamide gel electrophoresis. (2008)
  • Author(s): Tanaka S, Sakai A, Kimura K, Yoshida H, Fushitani H, Ogata A, Miyamoto A, Fukushima M, Wada A, Tanigawa N
  • Journal Title: Int.J.Oncol 33 Pages: 361-370
  • Peer Reviewed
• [Journal Article] Role of gp91phox-containing NADPH oxidase in left ventricular remodeling induced by intermittent hypoxic stress. (2008)
  • Author(s): Hayashi T, Yamashita C, Matsumoto C, Kwak CJ, Fujii K, Hirata T, Miyamura M, Mori T, Ukimura A, Okada Y, Matsumura Y, Kitaura Y
  • Journal Title: Am.J.Physiol.Heart Circ.Physiol 294
  • Peer Reviewed
• [Journal Article] Proteomic analysis of the basic proteins in 5-fluorouracil resistance of human colon cancer cell line using the radical-free and highly reducing method of two-dimensional polyacrylamide gel electrophoresis. (2008)
  • Author(s): Tanaka, S.
  • Journal Title: Int. J. Oncol. 33 Pages: 361-370
  • Peer Reviewed
• [Journal Article] Role of gp91phox-containing NADPH oxidase in left ventricular remodeling induced by intermittent hypoxic stress. (2008)
  • Author(s): Hayashi T
  • Journal Title: Am. J. Physiol. Heart Circ. Physiol. 294
  • Peer Reviewed
• [Presentation] 薬剤耐性を獲得した大腸癌細胞におけるプロテオーム解析 (2009)
  • Author(s): 境晶子
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィッコ横浜
  • Year and Date: 2009-12-09
• [Presentation] 薬剤耐性を獲得した大腸癌細胞におけるプロテオーム解析 (2009)
  • Author(s): 境晶子
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシィフィコ横浜(神奈川)
  • Year and Date: 2009-12-09
• [Presentation] 大腸癌細胞株における薬剤耐性獲得のプロテオーム解析-O-GlcNAc修飾に注目して (2008)
  • Author(s): 境晶子
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会の合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2008-12-11