Functional and structural analyses of novel hybrid type polyketide synthase Steely

• Project/Area Number: 20510196
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Living organism molecular science
• Research Institution: Hokkaido University
• Principal Investigator: SAITO Tamao Hokkaido University, 理工学部, 准教授 (30281843)
• Co-Investigator(Kenkyū-buntansha): MORITA Naoki (独)産業技術総合研究所, 生物プロセス研究部門分子生物工学研究グループ, 研究グループ長 (60371085)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 二次代謝産物 / ポリケタイド合成酵素 / 細胞性粘菌 / ポリケタイド

Research Abstract

The soil microorganism, Dictyostelium, has novel hybrid-type polyketide synthase (PKS) known as 'Steely' ; Steely enzyme is formed by the fusion of type I and type III PKSs. In this study, we examined how these two different enzymes work together and what are the products of Steely enzymes. Finally, we examined the biological functions of these products.

Research Products

• [Journal Article] Dictyostelium hybrid polyketide synthase, SteelyA, produces 4-methyl-5-pentylbenzene-1,3-diol and induces spore maturation (2011)
  • Author(s): Narita TB., Koide K., Morita N., Saito T.
  • Journal Title: FEMS Microbiology Letters 319(1) Pages: 82-87
  • NAID: 120005886086
  • Peer Reviewed
• [Journal Article] Dictyostelium hybrid polyketide synthase, Steely A, produces 4-methyl-5-pentylbenzene-1,3-diol and induces spore maturation (2011)
  • Author(s): Narita, TB., Koide, K., Morita, N., Saito, T.
  • Journal Title: FEMS Microbiology Letters Volume: (in press 未定)
  • Peer Reviewed
• [Journal Article] Migration of Dictyostelium slugs : anterior-like cells may provide the motive force for the prespore zone (2009)
  • Author(s): Rieu J-P., Saito T., Delanoe-Ayari H., Sawada Y., Kay RR.
  • Journal Title: Cell Motility and the Cytoskeleton 66 Pages: 1073-1086
  • Peer Reviewed
• [Journal Article] 土壌微生物・細胞性粘菌のポリケタイド合成酵素-新規ハイブリッド型酵素Steelyを中心として- (2009)
  • Author(s): 齊藤玉緒
  • Journal Title: 化学と生物 47 Pages: 22-27
• [Journal Article] Migration of Dictyostelium slugs : anterior-like cells may provide the motive force for the prespore zone (2009)
  • Author(s): Rieu, J-P., Saito, T., Delanoe-Ayari, H., Sawada, Y., Kay, RR.
  • Journal Title: Cell Motility and the Cytoskeleton 66 Pages: 1073-1086
  • Peer Reviewed
• [Journal Article] DIF-1 induces the basal disc of the Dictyostelium fruiting body (2008)
  • Author(s): Saito T., Kato A., Kay RR
  • Journal Title: Dev.Biol 317 Pages: 444-453
  • Peer Reviewed
• [Journal Article] DIF-1 induces the basal disc of the Dictyostelium fruiting body (2008)
  • Author(s): Saito, T., Kato, A., and Kay, RR
  • Journal Title: Dev. Biol 317 Pages: 444-453
  • Peer Reviewed
• [Presentation] 細胞性粘菌における新規ハイブリッド型酵素SteelyAの解析 (2011)
  • Author(s): 成田隆明、小出康太、森田直樹、齊藤玉緒
  • Organizer: 日本農芸化学会2011年度大会
  • Place of Presentation: 京都女子大学
  • Year and Date: 2011-03-27
• [Presentation] 細胞性粘菌における新規ハイブリッド型酵素ポリケタイド合成酵素Steelyの分子進化的解析 (2011)
  • Author(s): 原悠樹、田中亨、加藤敦之、齊藤玉緒
  • Organizer: 日本農芸化学会2011年度大会
  • Place of Presentation: 京都女子大学
  • Year and Date: 2011-03-27
• [Presentation] Expression profile of Ppolyketide Ssynthase genes in Dictyosteliu discoideum (2010)
  • Author(s): Akabane, K., Saito T.
  • Organizer: Annual International Dictyostelium Meeting
  • Place of Presentation: カーディフ大学(英)
  • Year and Date: 2010-08-04
• [Presentation] Expression profile of polyketide synthase genes in Dictyostelium discoideum (2010)
  • Author(s): Akabane, K., Saito, T.
  • Organizer: Annual International Dictyostelium Meeting
  • Place of Presentation: カーディフ大学(英)
  • Year and Date: 2010-08-04
• [Presentation] Expression profile of Polyketide Synthasegenes in Dictyostelium discoideum (2009)
  • Author(s): Narita T. Akabane K., Saito T.
  • Organizer: The third Asian plant lipids symposium
  • Place of Presentation: 横浜情報文化センター(横浜)
  • Year and Date: 2009-11-28
• [Presentation] Expression profile of Polyketide Synthase genes in Dictyostelium discoideum (2009)
  • Author(s): 成田隆明, 赤羽根一樹, 齊藤玉緒
  • Organizer: 第3回アジア植物脂質シンポジウム
  • Place of Presentation: 横浜情報文化センター(横浜)
  • Year and Date: 2009-11-28
• [Presentation] Functional analysis of Steely polyketide synthase (2009)
  • Author(s): 齊藤玉緒
  • Organizer: The third Asian plant lipids symposium
  • Place of Presentation: 横浜情報文化センター
  • Year and Date: 2009-11-27
• [Presentation] Functional analysis of Steely polyketide synthase (2009)
  • Author(s): 齊藤玉緒
  • Organizer: 第3回アジア植物脂質シンポジウム
  • Place of Presentation: 横浜情報文化センター(横浜)
  • Year and Date: 2009-11-27
• [Presentation] Functional analysis of Steely polyketide synthase (2008)
  • Author(s): Saito T., Kay RR.
  • Organizer: International Dictyostelium Conference.
  • Place of Presentation: Tsukuba, Japan
  • Year and Date: 2008-09-16
• [Presentation] Functional analysis of Steely polyketide synthase (2008)
  • Author(s): T. Saito and RR. Kay
  • Organizer: International Dictyostelium Conference
  • Place of Presentation: Tsukuba, Japan
  • Year and Date: 2008-09-16
• [Presentation] 細胞性粘菌のポリケタイド合成酵素 (2008)
  • Author(s): 齊藤玉緒
  • Organizer: 第3回化学生態学研究会
  • Place of Presentation: 函館
  • Year and Date: 2008-07-04