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Upgrade of sample exchanger to accelerate protein X-ray crystal structure analysis

Research Project

Project/Area Number 20560251
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Intelligent mechanics/Mechanical systems
Research InstitutionHigh Energy Accelerator Research Organization

Principal Investigator

HIRAKI Masahiko  High Energy Accelerator Research Organization, 物質構造科学研究所, 研究機関講師 (20282676)

Co-Investigator(Kenkyū-buntansha) WAKATSUKI Soichi  高エネルギー加速器研究機構, 物質構造科学研究所, 教授 (00332114)
KATO Ryuichi  高エネルギー加速器研究機構, 物質構造科学研究所, 准教授 (50240833)
KAWASAKI Masato  高エネルギー加速器研究機構, 物質構造科学研究所, 准教授 (00342600)
IGARASHI Noriyuki  高エネルギー加速器研究機構, 物質構造科学研究所, 准教授 (80300672)
MATSUGAKI Naohiro  高エネルギー加速器研究機構, 物質構造科学研究所, 助教 (50342598)
YAMADA Yusuke  高エネルギー加速器研究機構, 物質構造科学研究所, 助教 (20391708)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
KeywordsX線結晶構造解析 / タンパク質 / ロボティクス / 知能機械 / 校正 / 蛋白質
Research Abstract

We developed a sample exchange system that was able to insert sample holders to cassettes in a liquid nitrogen Dewar. The developed system was operated stably with only 0.1% troubles after the calibration of the system. In addition, the X-ray diffraction data of protein crystals stored by the developed system were enough for structure analysis.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (21 results)

All 2011 2010 2009 2008

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (19 results)

  • [Journal Article] Fully Automated Data Collection Using PAM and the Development of PAM/SPACE Reversible Cassettes2010

    • Author(s)
      Masahiko Hiraki, Shokei Watanabe, Leonard M.G.Chavas, Yusuke Yamada, Naohiro Matsugaki, Noriyuki Igarashi, Soichi Wakatsuki, Masahiro Fujihashi, Kunio Miki, Seiki Baba, Go Ueno, Masaki Yamamoto, Mamoru Suzuki, Atsushi Nakagawa, Nobuhisa Watanabe, Isao Tanaka
    • Journal Title

      American Institute of Physics Vol.1234

      Pages: 673-676

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Fully Automated Data Collection Using PAM and the Development of PAM/SPACE Reversible Cassettes2010

    • Author(s)
      Masahiko Hiraki
    • Journal Title

      American Institute of Physics

      Volume: 1234 Pages: 673-676

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] タンパク質結晶自動交換ロボットPAM2011

    • Author(s)
      平木雅彦、Leonard M.G.Chavas、山田悠介、松垣直宏、五十嵐教之、若槻壮市
    • Organizer
      日本結晶学会年会
    • Place of Presentation
      大阪大学コンベンションセンター、大阪
    • Year and Date
      2011-12-03
    • Related Report
      2010 Final Research Report
  • [Presentation] タンパク質結晶自動交換ロボットPAM2011

    • Author(s)
      平木雅彦
    • Organizer
      第24回日本放射光学会年会放射光科学合同シンポジウム
    • Place of Presentation
      茨城県、つくば国際会議場
    • Year and Date
      2011-01-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] タンパク質結晶自動交換ロボットPAM2011

    • Author(s)
      平木雅彦、Leonard M.G.Chavas、山田悠介、松垣直宏、五十嵐教之、若槻壮市
    • Organizer
      第24回日本放射光学会年会放射光科学合同シンポジウム
    • Place of Presentation
      つくば国際会議場、茨城
    • Related Report
      2010 Final Research Report
  • [Presentation] タンパク質結晶自動交換ロボットPAM2010

    • Author(s)
      平木雅彦
    • Organizer
      日本結晶学会年会
    • Place of Presentation
      大阪府、大阪大学コンベンションセンター
    • Year and Date
      2010-12-03
    • Related Report
      2010 Annual Research Report
  • [Presentation] Upgrade of High-throughput Sample Exchange Robots PAM and Development of PAM/SPACE Reversible Cassettes2010

    • Author(s)
      Masahiko Hiraki, Shokei Watanabe, Leonard M.G.Chavas, Yusuke Yamada, Naohiro Matsugaki, Noriyuki Igarashi, Soichi Wakatsuki, Masahiro Fujihashi, Kunio Miki, Seiki Baba, Go Ueno, Masaki Yamamoto, Mamoru Suzuki, Atsushi Nakagawa, Nobuhisa Watanabe, Isao Tanaka
    • Organizer
      回折構造生物学に関する国際シンポジウムISDSB2010
    • Place of Presentation
      パリSUD大学、フランス
    • Year and Date
      2010-05-28
    • Related Report
      2010 Final Research Report
  • [Presentation] Upgrade of High-throughput Sample Exchange Robots PAM and Development of PAM/SPACE Reversible Cassettes2010

    • Author(s)
      Masahiko Hiraki
    • Organizer
      ISDSB2010 (International Symposium on Diffraction Structural Biology)
    • Place of Presentation
      フランス、パリSUD大学
    • Year and Date
      2010-05-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] 蛋白質結晶自動交換ロボットの現状と今後の予定2010

    • Author(s)
      平木雅彦、Leonard M.G.Chavas、山田悠介、松垣直宏、五十嵐教之、若槻壮市
    • Organizer
      第27回PFシンポジウム
    • Place of Presentation
      つくば国際会議場、茨城
    • Year and Date
      2010-03-09
    • Related Report
      2010 Final Research Report
  • [Presentation] 結晶交換システムPAMの高度化2010

    • Author(s)
      平木雅彦、山田悠介、松垣直宏、Leonard M.G.Chavas、五十嵐教之、若槻壮市
    • Organizer
      第23回日本放射光学会年会放射光科学合同シンポジウム
    • Place of Presentation
      イーグレ姫路、兵庫
    • Year and Date
      2010-01-08
    • Related Report
      2010 Final Research Report
  • [Presentation] 第23回日本放射光学会年会放射光科学合同シンポジウム2010

    • Author(s)
      平木雅彦
    • Organizer
      結晶交換システムPAMの高度化
    • Place of Presentation
      姫路
    • Year and Date
      2010-01-08
    • Related Report
      2009 Annual Research Report
  • [Presentation] タンパク質結晶交換ロボットPAM2010

    • Author(s)
      平木雅彦、Chavas Leonard M.G.、山田悠介、松垣直宏、五十嵐教之、若槻壮市
    • Organizer
      PF研究会「放射光利用による構造生物の将来像」
    • Place of Presentation
      KEK小林ホール、茨城
    • Related Report
      2010 Final Research Report
  • [Presentation] Current status and developments of macromolecular crystallography beamlines at the Photon Factory2009

    • Author(s)
      Masahiko Hiraki, Leonard Chavas, Yusuke Yamada, Naohiro Matsugaki, Noriyuki Igarashi, Soichi Wakatsuki
    • Organizer
      アジア結晶学会AsCA2009
    • Place of Presentation
      北京Jing Yi Hotel,中国
    • Year and Date
      2009-10-24
    • Related Report
      2010 Final Research Report
  • [Presentation] Current status and development of macromolecular crystallography beamlines at the Photon Factory2009

    • Author(s)
      Masahiko HIRAKI
    • Organizer
      AsCA2009 (Joint Conference of the Asian Crystallographic Association and Chinese Crystallographic Society)
    • Place of Presentation
      北京(中国)
    • Year and Date
      2009-10-24
    • Related Report
      2009 Annual Research Report
  • [Presentation] Fully-automated data collection using PAM and development of PAM/SPACE reversible cassettes2009

    • Author(s)
      Masahiko Hiraki, Shokei Watanabe, Leonard M.G.Chavas, Yusuke Yamada, Naohiro Matsugaki, Noriyuki Igarashi, Soichi Wakatsuki, Masahiro Fujihashi, Kunio Miki, Seiki Baba, Go Ueno, Masaki Yamamoto, 鈴木守, Atsushi Nakagawa, Nobuhisa Watanabe, Isao Tanaka
    • Organizer
      放射光装置に関する国際会議SRI2009
    • Place of Presentation
      メルボルン国際会議場,オーストラリア
    • Year and Date
      2009-10-01
    • Related Report
      2010 Final Research Report
  • [Presentation] Fully-automated data collection using PAM and development of PAM/SPACE reversible cassettes2009

    • Author(s)
      Masahiko HIRAKI
    • Organizer
      SRI09 (The 10th International Conference on Synchrotron Radiation Instrumentation)
    • Place of Presentation
      メルボルン(オーストラリア)
    • Year and Date
      2009-10-01
    • Related Report
      2009 Annual Research Report
  • [Presentation] PF構造生物ビームラインにおける全自動解説データ収集の実現への取り組み2009

    • Author(s)
      平木雅彦、山田悠介、松垣直宏、五十嵐教之、若槻壮市
    • Organizer
      第26回PFシンポジウム
    • Place of Presentation
      つくば国際会議場、茨城
    • Year and Date
      2009-03-25
    • Related Report
      2010 Final Research Report
  • [Presentation] PF構造生物ビームラインにおける全自動回折データ収集の実現への取り組み2009

    • Author(s)
      平木雅彦
    • Organizer
      第26回PFシンポジウム
    • Place of Presentation
      エポカルつくば(茨城県つくば市)
    • Year and Date
      2009-03-25
    • Related Report
      2008 Annual Research Report
  • [Presentation] タンパク質結晶交換ロボットによる測定の自動化とサンプル用カセットの共通化2009

    • Author(s)
      平木雅彦、山田悠介、松垣直宏、五十嵐教之、若槻壮市、藤橋雅宏、上野剛、馬場清喜、鈴木守
    • Organizer
      第22回PF日本放射光学会年会放射光科学合同シンポジウム
    • Place of Presentation
      東京大学山上会館、東京
    • Year and Date
      2009-01-11
    • Related Report
      2010 Final Research Report
  • [Presentation] タンパク質結晶交換ロボットによる測定の自動化とサンプル用カセットの共通化2009

    • Author(s)
      平木雅彦
    • Organizer
      第22回 日本放射光学会年会放射光科学合同シンポジウム
    • Place of Presentation
      東京大学(東京都文京区)
    • Year and Date
      2009-01-11
    • Related Report
      2008 Annual Research Report
  • [Presentation] Approach for auto-mated data collection at the Photon Factory macromolecular crystal-lography beamlines2008

    • Author(s)
      Masahiko Hiraki, Naohiro Matsugaki, Yusuke Yamada, Noriyuki Igarashi, Yurii Gaponov, Shokei Watanabe, Wataru Uda, Kumiko Sasajima, Nobuo pHonda, Soichi Wakatsuki
    • Organizer
      国際結晶学会IUCr2008
    • Place of Presentation
      グランキューブ大阪国際会議場、大阪
    • Related Report
      2010 Final Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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