Development of highly sensitive immunochromato method using surfactant

• Project/Area Number: 20560731
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biofunction/Bioprocess
• Research Institution: Okayama University of Science
• Principal Investigator: NAGATANI Naoki Okayama University of Science, 工学部, 准教授 (90351072)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: バイオセンサー / イムノクロマト法 / 界面活性剤 / 金ナノ粒子 / 抗原抗体反応 / polymerase chain reaction(PCR)

Research Abstract

Immunochromato method that is in widespread use for pregnancy and influenza diagnosis is one of the promising tools for the development of easy-handling biosensors. To improve the detection sensitivity of the Immunochromato method were examined using a diluted solution containing surfactant. As a result, s-IgA (secretory immunoglobulin A) in saliva that was the stress marker and the influenza virus could be detected in high sensitivity by using a diluted solution containing 3～5%(wt) nonionic surfactant.

Research Products

• [Journal Article] Immunochromatographic assay using gold nanoparticles for measuring salivary secretory IgA in dogs as a stress marker (2009)
  • Author(s): Aki Takahashi, Shigeru Uchiyama, Yuya Kato, Teruko Yuhi, Hiromi Ushijima, Makoto Takezaki, Toshihiro Tominaga, Yoshiko Moriyama, Kunio Takeda, Toshiro Miyahara, Naoki Nagatani
  • Journal Title: Sci.Technol.Adv.Mater 10
  • Peer Reviewed
• [Journal Article] Immunochromatographic assay using gold nanoparticles for measuring salivary secretory IgA in dogs as a stress marker (2009)
  • Author(s): Aki Takahashi, Shigeru Uchiyama, Yuya Kato, Teruko Yuhi, Hiromi Ushijima, Makoto Takezaki, Toshihiro Tominaga, Yoshiko Moriyama, Kunio Takeda, Toshirou Miyahara, Naoki Nagatani
  • Journal Title: Science and Technology Advance Materials 10
  • Peer Reviewed
• [Presentation] 界面活性剤を含んだ展開液のイムノクロマ法への影響 (2011)
  • Author(s): 永谷尚紀、遠藤智史、牛島ひろみ、由比光子、宮原敏郎
  • Organizer: 日本化学会第91春季大会
  • Place of Presentation: 神奈川大学横浜キャンパス
  • Year and Date: 2011-03-28
• [Presentation] 界面活性剤を含んだ展開液のイムノクロマ法への影響 (2011)
  • Author(s): 永谷尚紀、遠藤智史、牛島ひろみ、由比光子、宮原敏郎
  • Organizer: 日本化学会第91春季大会
  • Place of Presentation: 神奈川大学 横浜キャンパス
  • Year and Date: 2011-03-28
• [Presentation] 界面活性剤が抗原抗体反応に及ぼす影響 (2009)
  • Author(s): 遠藤智史、高橋亜希、由比光子、牛島ひろみ・永谷尚紀、宮原敏郎
  • Organizer: 第20回化学とマイクロ・ナノシステム研究会
  • Place of Presentation: 金沢エクセルホテル東急、石川四高記念文化交流館
  • Year and Date: 2009-11-08
• [Presentation] 界面活性剤の抗原-抗体反応への影響(イムノクロマト法) (2009)
  • Author(s): 永谷尚紀、遠藤智史、高橋亜希、由比光子、牛島ひろみ、宮原敏郎
  • Organizer: 第62回コロイドおよび界面化学討論会
  • Place of Presentation: 岡山理科大学
  • Year and Date: 2009-09-17
• [Presentation] イムノクロマト法に対する界面活性剤の影響 (2008)
  • Author(s): 石井仁、高橋亜希、由比光子、牛島ひろみ、森山佳子、竹田邦雄、宮原敏郎、永谷尚紀
  • Organizer: 2008年日本化学会西日本大会
  • Place of Presentation: 長崎大学
  • Year and Date: 2008-11-15
• [Presentation] Immunochromatograhic assy using gold nanoparticles for measuring salivary secretory IgA in dogs as stress marker (2008)
  • Author(s): Aki Takahashi, Shigeru Uchiyama, Yuuya Kato, Teruko Yuhi, Hiromi Ushijima, Makoto Takezaki, Toshihiro Tominaga, Yoshiko Moriyama, Kunio Takeda, Toshirou Miyahara, Naoki Nagatani
  • Organizer: JAIST Nano Technology Symposium 2008
  • Place of Presentation: Ishikawa high-tech conference center, Ishikawa
  • Year and Date: 2008-10-24
• [Presentation] Immunochromatograhic assy using gold nanoparticles for measuring salivary secretory IgA in dogs as stress marker (2008)
  • Author(s): Aki Takahashi, Shigeru Uchiyama, Yuuya Kato, Teruko Yuhi, Hiromi Ushijima, Makoto Takezaki, Toshihiro Tominaga, Yoshiko Moriyama, Kunio Takeda, Toshirou Miyahara, Naoki Nagatani
  • Organizer: JAIST Nano Technology Symposium 2008 (NT2008)
  • Place of Presentation: Ishikawa high-tech conference center, Ishikaw
  • Year and Date: 2008-10-24
• [Presentation] Effect of terhalose and surfactant on immunochromatographic assay (2008)
  • Author(s): Naoki Nagatani, Aki Takahashi, Jin Ishii, Yuuta Toshine, Yuya Kato, Hiromi Ushijima, Makoto Takezaki, Yoshiko Moriyama, Kunio Takeda, Toshihiro Tominaga, Toshirou Miyahara
  • Organizer: Pacific Rim Meeting on Electrochemical and Solid-State Science
  • Place of Presentation: Honolulu, Hawaii, Hilton Hawaiian Village
  • Year and Date: 2008-10-15
• [Presentation] Effect of trehalose and surfactant on immunochromatograhic assay (2008)
  • Author(s): Naoki Nagatani, Aki Takahashi, Jin Ishii, Yuuta Toshine, Yuuya Kato, Hiromi Ushijima, Makoto Takezaki, Yoshiko Moriyama, Kunio Takeda, Toshihiro Tominaga, Toshirou Miyahara
  • Organizer: PRiME2008 (Pacific Rim Meeting on Electrochemical and Solid-state Science)
  • Place of Presentation: Honolulu, Hawaii, Hilton Hawaiian Village
  • Year and Date: 2008-10-15
• [Presentation] 界面活性剤によるイムノクロマト法の高感度化 (2008)
  • Author(s): 永谷尚紀、高橋亜希、石井仁、利根悠太、由比光子、牛島ひろみ、宮原敏郎
  • Organizer: 化学工学会第40回秋季大会
  • Place of Presentation: 東北大学川内北キャンパス
  • Year and Date: 2008-09-26
• [Presentation] 界面活性剤によるイムノクロマト法の高感度化 (2008)
  • Author(s): 永谷尚紀, 高橋亜希, 石井仁, 利根悠太, 由比光子, 牛島ひろみ, 宮原敏郎
  • Organizer: 化学工学会第40回秋季大会
  • Place of Presentation: 東北大学 川内北キャンパス
  • Year and Date: 2008-09-26
• [Presentation] 唾液中バイオマーカー測定用イムノクロマトテストストリップ (2008)
  • Author(s): 高橋亜希、由比光子、牛島ひろみ、宮原敏郎、永谷尚紀
  • Organizer: 第45回化学関連支部合同九州大会
  • Place of Presentation: 北九州国際会議場
  • Year and Date: 2008-07-05
• [Book] 農産物・食品検査法の新展開(監修山本重夫)(次の項目を担当第II編サンプリング検査法の技術第9章イムノクロマト法の高感度技術と新展開) (2010)
  • Author(s): 永谷尚紀
  • Publisher: シーエムシー出版
• [Book] 農産物・食品検査法の新展開*第9章イムノクロマト法の高感度技術と新展開(p159-166)を執筆 (2010)
  • Author(s): 監修山本重夫
  • Total Pages: 265
  • Publisher: シーエムシー出版
• [Remarks] 招待講演
• [Remarks] 永谷尚紀、イムノクロマト法を用いるウイルス検査法、第15回OUS技術セミナー2009、2010年1月13日、岡山国際交流センター