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Genome-wide screening of localized RNAs in Schizosaccharomyces pombe

Research Project

Project/Area Number 20570005
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Genetics/Genome dynamics
Research InstitutionKumamoto University (2008, 2010)
Otsuma Women's University Junior College Division (2009)

Principal Investigator

TAKEUCHI Tomoko  Kumamoto University, 短期大学部・家政科, 准教授 (20294548)

Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords分裂酵母 / RNA / 局在化 / ゲノムワイド / non-coding / 可視化 / スクリーニング / DNA領域 / non-codin / non -coding / DNA 領域
Research Abstract

In screening of new 5,316 RNAs (6,520 in total), we found 39 candidate RNAs showing subcellular localization. 25 RNAs showed localization as dots in the nucleus, and 5 RNAs and 9 RNAs were localized in the DNA region and in the nucleolus region of the nucleus, respectively. Analysis of 2 RNAs localized in the DNA region revealed that RNA sequences required for the localization were different between the two RNAs. We obtained data suggesting that the RNA sequences of the two RNAs have ability to regulate gene expression.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (14 results)

All 2011 2010 2009 2008 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (8 results) Remarks (4 results)

  • [Journal Article] EGD1 (β-NAC) mRNA is localized in a novel cytoplasmic structure in Saccharomyces cerevisiae.2011

    • Author(s)
      Sachiko Hayashi, Tomoko Andoh, Tokio Tani
    • Journal Title

      Genes Cells vol.16

      Pages: 316-329

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] EGD1 (β-NAC) mRNA is localized in a novel cytoplasmic structure in Saccharomyces cerevisiae.2011

    • Author(s)
      Sachiko Hayashi
    • Journal Title

      Genes to Cells

      Volume: Vol.16 Pages: 316-329

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 分裂酵母spo9^+の3'UTR内に存在する核内局在化(核係留)RNA配列の解析2010

    • Author(s)
      渕田綾子
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸ポートアイランド
    • Related Report
      2010 Final Research Report
  • [Presentation] 分裂酵母における核内局在化(核係留)RNA配列の同定と解析2010

    • Author(s)
      渕田綾子
    • Organizer
      第12回日本RNA学会年会
    • Place of Presentation
      一橋記念講堂(東京都)
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] 分裂酵母spo9^+の3'UTR内に存在する核内局在化(核係留)RNA配列の解析2010

    • Author(s)
      渕田綾子
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会
    • Place of Presentation
      神戸ポートアイランド
    • Related Report
      2010 Annual Research Report
  • [Presentation] 分裂酵母における細胞内局在化RNAの網羅的探索と解析2009

    • Author(s)
      渕田綾子
    • Organizer
      RNAフロンティアミーティング2009
    • Place of Presentation
      IPC生産性国際交流センター
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] 分裂酵母における細胞内局在化RNAの網羅的探索と新規核内局在化non-coding RNAの解析2008

    • Author(s)
      大庭沙耶歌
    • Organizer
      第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2008-12-12
    • Related Report
      2010 Final Research Report
  • [Presentation] 分裂酵母における細胞内局在化RNAの網羅的探索と新規核内局在化non-coding RNAの解析2008

    • Author(s)
      大庭沙耶歌
    • Organizer
      第31回日本分子生物学会年会、第81回日本生化学会大会 合同大会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2008-12-12
    • Related Report
      2008 Annual Research Report
  • [Presentation] 分裂酵母における網羅的な局在化RNAのスクリーニングと遺伝子発現調節機構の解析2008

    • Author(s)
      大庭沙耶歌
    • Organizer
      第60回日本細胞生物学会大会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2008-06-30
    • Related Report
      2010 Final Research Report
  • [Presentation] 分裂酵母における網羅的な局在化RNAのスクリーニングと遺伝子発現調節機構の解析2008

    • Author(s)
      大庭 沙耶歌
    • Organizer
      第60回 日本細胞生物学会大会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2008-06-30
    • Related Report
      2008 Annual Research Report
  • [Remarks] ホームページ等

    • URL

      http://www.paw.hi-ho.ne.jp/t-takeu/

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.paw.hi-ho.ne.jp/t-takeu

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.paw.hi-ho.ne.jp/t-takeu

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://aster.sci.kumamoto-u.ac.jp/~biohome/staff/tani/andoh/AndohHP2007/index.html

    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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