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Analysis of calcification mechanism in Haptophyta, using cDNA macroarray

Research Project

Project/Area Number 20570059
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphology/Structure
Research InstitutionTokyo University of Pharmacy and Life Science

Principal Investigator

FUJIWARA Shoko  東京薬科大学, 生命科学部, 准教授 (30266895)

Co-Investigator(Kenkyū-buntansha) TSUZUKI Mikio  東京薬科大学, 生命科学部, 教授 (70155430)
Project Period (FY) 2008 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords石灰化 / cDNAアレイ / ハプト藻 / 円石藻 / バイオミネラリゼーション / ハプト薬 / マクロアレイ
Research Abstract

cDNA macroarray analyses were performed in a pair of coccolith-bearing and naked strains of Pleurochrysis haptonemofera, cultures grown under various conditions to alter calcification rate, and wild type and coccolith-deficient mutants, PS2^-and PS3^-, of P. carterae. The results suggested that genes including those for carbonic anhydrase and keratin-associated protein are involved in coccolith formation.

Report

(6 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report   Self-evaluation Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (56 results)

All 2012 2011 2010 2009 2008 Other

All Journal Article (24 results) (of which Peer Reviewed: 12 results) Presentation (25 results) Book (7 results)

  • [Journal Article] Effects of granule-bound starch synthase I-defective mutation on the morphology and structure of pyrenoidal starch in Chlamydomonas2011

    • Author(s)
      Izumo, A., S. Fujiwara, T. Sakurai, S. G. Ball, Y. Ishii, H. Ono, M. Yoshida, N. Fujita, Y. Nakamura, A. Buleon and M. Tsuzuki
    • Journal Title

      Plant Sci

      Volume: 180 Pages: 238-45

    • NAID

      130006998251

    • Related Report
      2011 Final Research Report
  • [Journal Article] Rapid biotransformation of arsenate into oxo-arsenosugars by a freshwater unicellular green alga, Chlamydomonas reinhardtii.2011

    • Author(s)
      Miyashita, S., S. Fujiwara, M. Tsuzuki and T. Kaise
    • Journal Title

      Biosci. Biotechnol. Biochem

      Volume: 75 Pages: 522-30

    • NAID

      10028201798

    • Related Report
      2011 Final Research Report
  • [Journal Article] Rapid Biotransformation of Arsenate into Oxo-Arsenosugars by a Freshwater Unicellular Green Alga, Chlamydomonas reinhardtii2011

    • Author(s)
      Miyashita, S., et al
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 75 Issue: 3 Pages: 522-530

    • DOI

      10.1271/bbb.100751

    • NAID

      10028201798

    • ISSN
      0916-8451, 1347-6947
    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The primitive rhodophyte Cyanidioschyzon merolae contains a semiamylopectin-type but not an amylose-type α-glucan.2010

    • Author(s)
      Hirabaru, C., A. Izumo, S. Fujiwara, Y. Tadokoro, T. Shimonaga, M. Konishi, M. Yoshida, N. Fujita, Y. Nakamura, M. Yoshida, T. Kuroiwa and M. Tsuzuki
    • Journal Title

      Plant Cell Physiol

      Volume: 51 Pages: 682-93

    • NAID

      10027348891

    • Related Report
      2011 Final Research Report
  • [Journal Article] Carbohydrate metabolism in the mutants of the cyanobacterium Synechococcus elongatus PCC 7942 defective in glycogen synthesis2010

    • Author(s)
      Suzuki, E., H. Ohkawa, K. Moriya, T. Matsubara, Y. Nagaike, I. Iwasaki, S. Fujiwara, M. Tsuzuki and Y. Nakamura
    • Journal Title

      Appl. Environ. Microbiol

      Volume: 76 Pages: 3153-9

    • Related Report
      2011 Final Research Report
  • [Journal Article] Effects of Ca and Mg on growth and calcification of the coccolithophorid Pleurochrysis haptonemofera : Ca-requirement for cell division in coccolith-bearing cells and for normal coccolith-formation with acidic polysaccharides2010

    • Author(s)
      Katagiri, F., Y. Takatsuka, S. Fujiwara and M. Tsuzuki
    • Journal Title

      Mar. Biotechnol

      Volume: 12 Pages: 42-51

    • Related Report
      2011 Final Research Report
  • [Journal Article] The primitive rhodophyte Cyanidioschyzon merolae contains a semiamylopectin-type, but not an amylose-type α-glucan.2010

    • Author(s)
      Hirabaru, C., A.Izumo, S.Fujiwara, Y.Tadokoro, T.Shimonaga, M.Konishi, M.Yoshida, N.Fujita, Y.Nakamura, M.Yoshida, T.Kuroiwa, M.Tsuzuki
    • Journal Title

      Plant Cell Physiol. 51

      Pages: 682-693

    • NAID

      10027348891

    • Related Report
      2010 Self-evaluation Report
  • [Journal Article] Carbohydrate metabolism in the mutants of the cyanobacterium Synechococcus elongatus PCC 7942 defective in glycogen synthesis.2010

    • Author(s)
      Suzuki, E., H.Ohkawa, K.Moriya, T.Matsubara, Y.Nagaike, I.Iwasaki, S.Fujiwara, M.Tsuzuki, Y.Nakamura
    • Journal Title

      Appl.Environ.Microbiol. 76

      Pages: 3153-3159

    • Related Report
      2010 Self-evaluation Report
  • [Journal Article] Effects of Ca and Mg on growth and calcification of the coccolithophorid Pleurochrysis haptonemofera : Ca-requirement for cell division in coccolith-bearing cells and for normal coccolith-formation with acidic polysaccharides.2010

    • Author(s)
      Katagiri, F., Y.Takatsuka, S.Fujiwara, M.Tsuzuki
    • Journal Title

      Mar.Biotechnol. 12

      Pages: 42-51

    • Related Report
      2010 Self-evaluation Report
  • [Journal Article] Carbohydrate metabolism in the mutants of the cyanobacterium Synechococcus elongatus PCC 7942 defective in glycogen synthesis.2010

    • Author(s)
      Suzuki, E., et al.
    • Journal Title

      Appl.Environ.Microbiol.

      Volume: 76 Pages: 3153-3159

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The primitive rhodophyte Cyanidioschyzon merolae contains a semiamylopectin-type, but not an amylose-type α-glucan.2010

    • Author(s)
      Hirabaru, C., et al.
    • Journal Title

      Plant Cell Physiol.

      Volume: 51 Pages: 682-693

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of granule-bound starch synthase I-defective mutation on the morphology and structure of pyrenoidal starch in Chlamydomonas.2010

    • Author(s)
      Izumo, A., et al.
    • Journal Title

      Plant Sci.

      Volume: 180 Pages: 238-245

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of Ca and Mg on growth and calcification of the coccolithophorid Pleuro chrysis haptonemofera : Ca-requirement for cell division in coccolith-bearing cells and for normal coccolith-formation with acidic polysaccharides.2010

    • Author(s)
      Katagiri, F., et al.
    • Journal Title

      Mar.Biotechnol. 12

      Pages: 42-51

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rapid determination of arsenic species in freshwater organisms from the arsenic-rich Hayakawa River in Japan using HPLC-ICP-MS.2009

    • Author(s)
      Miyashita, S., et al.
    • Journal Title

      Chemosphere 75

      Pages: 1065-1073

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural and physiological studies on the storage β-polyglucan of haptophyte Pleurochrysis haptonemofera2008

    • Author(s)
      Hirokawa, Y., S. Fujiwara, M. Suzuki, T. Akiyama, M. Sakamoto, S. Kobayashi and M. Tsuzuki
    • Journal Title

      Planta

      Volume: 227 Pages: 589-599

    • Related Report
      2011 Final Research Report
  • [Journal Article] Variation in storage α-glucans of the Porphyridiales(Rhodophyta)2008

    • Author(s)
      Shimonaga, T., M. Konishi, Y, Oyama, S. Fujiwara, A. Satoh, N. Fujita, C. Colleoni, A. Buleon, J.-L. Putaux, S. G. Ball, A. Yokoyama, Y. Hara, Y. Nakamura and M. Tsuzuki
    • Journal Title

      Plant Cell Physiol

      Volume: 49 Pages: 103-116

    • NAID

      10027495338

    • Related Report
      2011 Final Research Report
  • [Journal Article] Structural and physiological studies on the storage β-polyglucan of haptophyte Pleurochrysis haptonemofera.2008

    • Author(s)
      Hirokawa, Y., S.Fujiwara, M.Suzuki, T.Akiyama, M.Sakamoto, S.Kobayashi, M.Tsuzuki
    • Journal Title

      Planta 227

      Pages: 589-599

    • Related Report
      2010 Self-evaluation Report
  • [Journal Article] Variation in storage α-glucans of the Porphyridiales (Rhodophyta).2008

    • Author(s)
      Shimonaga, T., M.Konishi, Y, Oyama, S.Fujiwara, A.Satoh, N.Fujita, C.Colleoni, A.Buleon, J.-L.Putaux, S.G.Ball, A.Yokoyama, Y.Hara, Y.Nakamura, M.Tsuzuki
    • Journal Title

      Plant Cell Physiol. 49

      Pages: 103-116

    • NAID

      10027495338

    • Related Report
      2010 Self-evaluation Report
  • [Journal Article] Structural and physiological studies on the storage β-polyglucan of haptophyte Pleurochrysis hapto2008

    • Author(s)
      Hirokawa, Y., S. Fujiwara, M. Suzuki, T.
    • Journal Title

      Planta 227

      Pages: 589-599

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Variation in storage α-glucans of the Porphyridiales(Rhodophyta).2008

    • Author(s)
      Shimonaga, T., M. Konishi, Y, Oyama, S.
    • Journal Title

      Plant Cell Physiol. 49

      Pages: 103-116

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rapid biotransformation of arsenate into oxo-arsenosugars by a freshwater unicellular green alga, Chlamydomonas reinhardtii.

    • Author(s)
      Miyashita, S., et al.
    • Journal Title

      Biosci.Biotechnol.Biochem.

      Volume: (in press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Carbohydrate metabolism in the mutants of the cyanobacterium Synechococcus elongatus PCC 7942 defective in glycogen synthesis.

    • Author(s)
      Suzuki, E., et al.
    • Journal Title

      Appl.Environ.Microbiol. (in press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The primitive rhodophyte Cyanidioschyzon merolae contains a semiam ylopectin-type, but not an amylose-type α-glucan.

    • Author(s)
      Hirabaru, C., et al.
    • Journal Title

      Plant Cell Physiol. (in press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rapid determination of arsenic species in freshwater organisms from the arsenic-rich Hayakawa Rive :

    • Author(s)
      Miyashita, S., M. Shimoya, Y. Kamidate
    • Journal Title

      Chemospere in press

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] Synechocystis sp.PCC6803のfbaA遺伝子における二系統の発現誘導光シグナル2012

    • Author(s)
      岡田克彦, ら
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都
    • Year and Date
      2012-03-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] 円石藻Pleurochrysis carteraeにおけるココリス形成の分子生物学的解析2011

    • Author(s)
      遠藤博寿, ら
    • Organizer
      第6回バイオミネラリゼーション研究会
    • Place of Presentation
      東京
    • Year and Date
      2011-12-03
    • Related Report
      2011 Annual Research Report
  • [Presentation] 緑藻とシアノバクテリアにおけるヒ素に対する感受性と蓄積量2011

    • Author(s)
      近藤慶典, ら
    • Organizer
      第14回マリンバイオテクノロジー学会
    • Place of Presentation
      静岡
    • Year and Date
      2011-05-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] 円石藻Pleurochrysis carteraeにおけるココリス形成の分子生物学的解析2011

    • Author(s)
      遠藤博寿、笠島大貴、藤原祥子、都筑幹夫、猿渡和子、小暮敏博、長澤寛道
    • Organizer
      第6回バイオミネラリゼーションワークショップ
    • Place of Presentation
      東京
    • Related Report
      2011 Final Research Report
  • [Presentation] Chlamydomonas reinhardtiiのgranule-bound starch synthase欠損がピレノイドの形態及び構造に及ぼす影響2011

    • Author(s)
      櫻井俊宏, ら
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      仙台(要旨発表のみ)
    • Related Report
      2010 Annual Research Report
  • [Presentation] 円石藻のココリスの有機基板に含まれるタンパク質の同定と発現解析2010

    • Author(s)
      笠島大貴、遠藤博寿、長坂征治、藤原祥子、都筑幹夫、長澤寛道
    • Organizer
      第5回バイオミネラリゼーションワークショップ
    • Place of Presentation
      東京
    • Related Report
      2011 Final Research Report
  • [Presentation] Expression of phosphate transporter genes in Chlamydomonas reinhardtii.2010

    • Author(s)
      Murota, C., et al.
    • Organizer
      International Symposium "Microalgal Biotechnology-Food・Environment・Energy"
    • Place of Presentation
      東京
    • Related Report
      2010 Annual Research Report
  • [Presentation] Biotransformation of arsenate to arsenosugars by Chlamydomonas reinhardtii.2010

    • Author(s)
      Miyashita, S., et al.
    • Organizer
      International Symposium "Microalgal Biotechnology-Food・Environment・Energy"
    • Place of Presentation
      東京
    • Related Report
      2010 Annual Research Report
  • [Presentation] Relationship between As(V)resistance and deficiency in photosynthesis in Chlamydomonas reinhardtii.2010

    • Author(s)
      Murota, C., et al.
    • Organizer
      The 15^<th> International Congress of Photosynthesis
    • Place of Presentation
      北京
    • Related Report
      2010 Annual Research Report
  • [Presentation] 円石藻のココリスの有機基板に含まれるタンパク質の同定と発現解析2010

    • Author(s)
      笠島大貴, ら
    • Organizer
      第4回バイオミネラリゼーションワークショップ
    • Place of Presentation
      東京
    • Related Report
      2010 Annual Research Report
  • [Presentation] 緑藻クラミドモナスのリン酸トランスポーター遺伝子発現に対するヒ酸の影響2010

    • Author(s)
      室田知里, ら
    • Organizer
      第51回日本植物生理学会年会
    • Place of Presentation
      熊本
    • Related Report
      2009 Annual Research Report
  • [Presentation] 海産性植物プランクトン、円石藻の石灰化(ココリス形成)2009

    • Author(s)
      藤原祥子
    • Organizer
      第4回バイオミネラリゼーションワークショップ
    • Place of Presentation
      東京
    • Related Report
      2011 Final Research Report
  • [Presentation] 円石藻Pleurochrysis haptonemoferaの円石形成関連遺伝子の網羅的解析と環境因子の影響2009

    • Author(s)
      高塚由紀子、藤原祥子、須田邦裕、中島大輔、岡崎孝映、柴田大輔、田畑哲之、都筑幹夫
    • Organizer
      第12回マリンバイオテクノロジー学会
    • Place of Presentation
      東京
    • Related Report
      2011 Final Research Report
  • [Presentation] 円石藻Pleurochrysis haptonemoferaの石灰化に及ぼす環境因子の影響2009

    • Author(s)
      高塚由紀子、藤原祥子、片桐史朗、柴田大輔、田畑哲之、都筑幹夫
    • Organizer
      日本藻類学会第33回大会
    • Place of Presentation
      那覇
    • Related Report
      2011 Final Research Report 2010 Self-evaluation Report 2008 Annual Research Report
  • [Presentation] 円石藻Pleurochrysisの石灰化における2価金属イオンと重炭酸イオンの影響2009

    • Author(s)
      高塚由紀子、藤原祥子、片桐史朗、須田邦裕、柴田大輔、田畑哲之、都筑幹夫
    • Organizer
      第3回バイオミネラリゼーションワークショップ
    • Place of Presentation
      東京
    • Related Report
      2011 Final Research Report 2010 Self-evaluation Report
  • [Presentation] 海産性植物プランクトン、円石藻の石灰化(ココリス形成)2009

    • Author(s)
      藤原祥子
    • Organizer
      第4回バイオミネラリゼーションワークショップ
    • Place of Presentation
      東京(特別講演)
    • Related Report
      2010 Self-evaluation Report
  • [Presentation] 円石藻Pleurochrysis haptonemoferaの円石形成関連遺伝子の網羅的解析と環境因子の影響2009

    • Author(s)
      高塚由紀子, ら
    • Organizer
      第12回マリンバイオテクノロジー学会大会
    • Place of Presentation
      東京
    • Related Report
      2009 Annual Research Report
  • [Presentation] 海産性植物プランクトン、円石藻の石灰化(ココリス形成)(招待講演)2009

    • Author(s)
      藤原祥子
    • Organizer
      第4回バイオミネラリゼーションワークショップ
    • Place of Presentation
      東京
    • Related Report
      2009 Annual Research Report
  • [Presentation] 円石藻Pleurochrysis carteraeにおける遺伝子導入技術の確立の試み2009

    • Author(s)
      遠藤博寿, ら
    • Organizer
      第4回バイオミネラリゼーションワークショップ
    • Place of Presentation
      東京
    • Related Report
      2009 Annual Research Report
  • [Presentation] 円石藻Pleurochrysisの石灰化における2価金属イオンと重炭酸イオンの影響2008

    • Author(s)
      高塚由紀子, 藤原祥子, 片桐史朗ら
    • Organizer
      第3回バイオミネラリゼーションワークショップ
    • Place of Presentation
      東京
    • Year and Date
      2008-12-13
    • Related Report
      2008 Annual Research Report
  • [Presentation] 円石藻類Pleurochrysis carteraeのココリス形成に関わる遺伝子の探索2008

    • Author(s)
      長坂征治, 藤原祥子, 高塚由紀子ら
    • Organizer
      第3回バイオミネラリゼーションワークショップ
    • Place of Presentation
      東京
    • Year and Date
      2008-12-13
    • Related Report
      2008 Annual Research Report
  • [Presentation] マクロアレイを用いた円石藻の円石形成細胞特異的発現遺伝子の網羅的解析(論文賞受賞講演)2008

    • Author(s)
      藤原祥子, 広川安孝, 高塚由紀子ら
    • Organizer
      第11回マリンバイオテクノロジー学会大会
    • Place of Presentation
      京都
    • Year and Date
      2008-05-24
    • Related Report
      2008 Annual Research Report
  • [Presentation] マクロアレイを用いた円石藻の円石形成細胞特異的発現遺伝子の網羅的解析2008

    • Author(s)
      藤原祥子、広川安孝、高塚由紀子、須田邦裕、浅水恵理香、高柳賢利、柴田大輔、田畑哲之、都筑幹夫
    • Organizer
      第11回マリンバイオテクノロジー学会大会
    • Place of Presentation
      京都
    • Related Report
      2011 Final Research Report
  • [Presentation] マクロアレイを用いた円石藻の円石形成細胞特異的発現遺伝子の網羅的解析2008

    • Author(s)
      藤原祥子、広川安孝、高塚由紀子、須田邦裕、浅水恵理香、高柳賢利、柴田大輔、田畑哲之、都筑幹夫
    • Organizer
      第11回マリンバイオテクノロジー学会大会
    • Place of Presentation
      京都(論文賞授賞講演)
    • Related Report
      2010 Self-evaluation Report
  • [Presentation] 円石藻Pleurochrysis haptonemoferaの円石形成関連遺伝子の網羅的解析と環境因子の影響

    • Author(s)
      高塚由紀子、藤原祥子、須田邦裕、中島大輔、岡崎孝映、柴田大輔、田畑哲之、都筑幹夫
    • Organizer
      第12回マリンバイオテクノロジー学会
    • Related Report
      2010 Self-evaluation Report
  • [Book] Coccolith formation in the Haptophyta. In Marine Phytoplankton2009

    • Author(s)
      Fujiwara, S. and M. Tsuzuki
    • Publisher
      Nova Science Publishers, Inc
    • Related Report
      2011 Final Research Report
  • [Book] Coccolith formation in the Haptophyta. In Marine Phytoplankton (Eds.: Kersey, W.T.& S.P.Munger, ISBN : 978-1-60741-087-4)2009

    • Author(s)
      Fujiwara, S., M.Tsuzuki
    • Publisher
      Nova Science Publishers, Inc.
    • Related Report
      2010 Self-evaluation Report
  • [Book] Marine Phytoplanktonの一部2009

    • Author(s)
      Kersey, W.T., S.P.Munger編
    • Total Pages
      11
    • Publisher
      Nova Science Publishers, Inc.
    • Related Report
      2009 Annual Research Report
  • [Book] Arsenic metabolism in cyanobacteria. In Cyanobacteria : Toxicity, Ecology and Management

    • Author(s)
      Miyashita S., K. Kondo, M. Tsuzuki and S. Fujiwara
    • Publisher
      Nova Science Publishers, Inc.(in press)
    • Related Report
      2011 Final Research Report
  • [Book] 藻類ハンドブック(渡邊信監修)、第2章代謝と物質、第1節代謝と物質、5糖類-デンプン他

    • Author(s)
      藤原祥子、都筑幹夫
    • Publisher
      株式会社エヌ・ティー・エス(in press)
    • Related Report
      2011 Final Research Report
  • [Book] Cyanobacteria : Toxicity, Ecology and Managementの一部

    • Author(s)
      Miyashita S., et al
    • Publisher
      Nova Science Publishers(in press)
    • Related Report
      2011 Annual Research Report
  • [Book] 藻類ハンドブックの一部

    • Author(s)
      渡邊信監修
    • Publisher
      (株)エヌ・ティー・エス(in press)
    • Related Report
      2010 Annual Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

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