Study on the network among metal-related proteins for mechanisms and functions of vanadium ions.

• Project/Area Number: 20570070
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Animal physiology/Animal behavior
• Research Institution: Hiroshima University
• Principal Investigator: UEKI Tatsuya Hiroshima University, 大学院・理学研究科, 准教授 (10274705)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: ホヤ / バナジウム / 金属結合タンパク質 / 金属輸送体 / タンパク質間相互作用

Research Abstract

In order to reveal protein-protein interaction network among Vanabins and metal-related proteins in vanadocytes and blood plasma in a vanadium-rich ascidian Ascidia sydneiensis samea, we performed the screening for proteins that interact with known vanadium-binding proteins, and analyzed protein-protein interaction and metal-binding and -transfer activities.

Research Products

• [Journal Article] A novel vanadium transporter of the Nramp family expressed at the vacuole of vanadium-accumulating cells of the ascidian Ascidia sydneiensis samea. (2011)
  • Author(s): Ueki, T., Furuno, N., Michibata, H.
  • Journal Title: Biochim. Biophys. Acta 1810 Pages: 457-464
  • NAID: 120005753917
  • Peer Reviewed
• [Journal Article] Molecular mechanism of the transport and reduction pathway of vanadium in ascidians. Coord. (2011)
  • Author(s): Ueki, T., Michibata, H.
  • Journal Title: Chem.Rev. in press.
  • Peer Reviewed
• [Journal Article] Advances in research on the accumulation, redox behavior, and function of vanadium in ascidians. (2010)
  • Author(s): Michibata, H., Ueki, T.
  • Journal Title: Biomol. Concep. 1 Pages: 97-107
  • Peer Reviewed
• [Journal Article] Advances in research on the accumulation, redox behavior, and function of vanadium in ascidians (2010)
  • Author(s): 植木龍也
  • Journal Title: Biomolecular Concepts Volume: 1 Pages: 97-107
  • Peer Reviewed
• [Journal Article] Characterization of vanadium-binding sites of the vanadium-binding protein Vanabin2 by site-directed mutagenesis. (2009)
  • Author(s): Ueki, T., Kawakami, N., Toshishige, M., Matsuo, K., Gekko, K., Michibata, H.
  • Journal Title: Biochim. Biophys. Acta 1790 Pages: 1327-1333
  • Peer Reviewed
• [Journal Article] Identification and biochemical analysis of a homolog of a sulfate transporter from a vanadium-rich ascidian Ascidia sydneiensis samea. (2009)
  • Author(s): Ueki, T., Furuno, N., Xu, Q., Nitta, Y., Kanamori, K., Michibata, H.
  • Journal Title: Biochim. Biophys. Acta 1790 Pages: 1295-1300
  • Peer Reviewed
• [Journal Article] A novel vanadium reductase, Vanabin2, forms a possible cascade involved in electron transfer. (2009)
  • Author(s): Kawakami, N., Ueki, T., Amata, Y., Kanamori, K., Matsuo, K., Gekko, K., Michibata, H.
  • Journal Title: Biochim. Biophys. Acta 1794 Pages: 674-679
  • NAID: 120001433933
  • Peer Reviewed
• [Journal Article] A novel vanadium reductase, Vanabin2, forms a possible cascade involved in electron transfer (2009)
  • Author(s): 川上了史
  • Journal Title: Biochimica et Biophysica Acta 1790 Pages: 674-679
  • Peer Reviewed
• [Journal Article] Identification and biochemical analysis of a homolog of a sulfate transporter from a vanadium-rich ascidian Ascidia sydneiensis samea (2009)
  • Author(s): 植木龍也
  • Journal Title: Biochimica et Biophysica Acta 1790 Pages: 1295-1300
  • Peer Reviewed
• [Journal Article] Characterization of vanadium-binding sites of the vanadium-binding protein Vanabin2 by site-directed mutagenesis (2009)
  • Author(s): 植木龍也
  • Journal Title: Biochimica et Biophysica Acta 1790 Pages: 1327-1333
  • Peer Reviewed
• [Journal Article] Sequence variation of Vanabin2-like vanadium-binding proteins in blood cells of the vanadium-accumulating ascidian Ascidia sydneiensis samea, Biochim. (2008)
  • Author(s): Ueki, T., Satake, M., Kamino, K., Michibata, H.
  • Journal Title: Biophys. Acta 1780 Pages: 1010-1015
  • Peer Reviewed
• [Journal Article] Sequence variation of Vanabin2-like vanadium-binding proteins in blood cells of the vanadium-accumulating ascidian Ascidia sydneiensis samea (2008)
  • Author(s): 植木 龍也, 他
  • Journal Title: Biochimica et Biophysica Acta 1780・7-8 Pages: 1010-1015
  • NAID: 120001092484
  • Peer Reviewed
• [Journal Article] A novel vanadium reductase, Vanabin2, forms a possible cascade involved in electron transfer.
  • Author(s): 川上 了史, 植木 龍也, 他
  • Journal Title: Biochimica et Biophysica Acta (印刷中)
  • NAID: 120001433933
  • Peer Reviewed
• [Presentation] Molecular mechanism of the transport and reduction pathway of vanadium in ascidians. (2010)
  • Author(s): Ueki, T., Michibata, H.
  • Organizer: 第7回国際バナジウム化学生物学シンポジウム,招待講演
  • Place of Presentation: 富山
  • Year and Date: 2010-10-08
• [Presentation] Expressed sequence tag (EST) analysis of the intestine from the most vanadium-rich ascidian Ascidia gemmata, and their application to heavy metal biosorption system. (2010)
  • Author(s): Samino, S., Ueki, T., Michibata, H.
  • Organizer: 第7回国際バナジウム化学生物学シンポジウム
  • Place of Presentation: 富山
  • Year and Date: 2010-10-08
• [Presentation] Measurement and comparison of V(V)-reductase activity of Vanabin family of a vanadium-rich ascidian Ascidia sydneiensis samea. (2010)
  • Author(s): Kimizu, T., Ueki, T., Michibata, H.
  • Organizer: 第7回国際バナジウム化学生物学シンポジウム
  • Place of Presentation: 富山
  • Year and Date: 2010-10-08
• [Presentation] Identification of genes regulated by excess vanadium ions in ascidians, focusing on redox and accumulation of metals. (2010)
  • Author(s): Kume, S., Ueki, T., Michibata, H.
  • Organizer: 第7回国際バナジウム化学生物学シンポジウム,ポスター賞受賞
  • Place of Presentation: 富山
  • Year and Date: 2010-10-08
• [Presentation] Molecular mechanism of the transport and reduction pathway of vanadium in ascidians (2010)
  • Author(s): 植木龍也
  • Organizer: 第7回国際バナジウム化学・生物学シンポジウム
  • Place of Presentation: 富山市(招待講演)
  • Year and Date: 2010-10-08
• [Presentation] Expressed sequence tag(EST)analysis of the intestine from the most vanadium-rich ascidian Ascidia gemmata, and their application to heavy metal biosorption system (2010)
  • Author(s): 植木龍也
  • Organizer: 第7回国際バナジウム化学・生物学シンポジウム
  • Place of Presentation: 富山市
  • Year and Date: 2010-10-08
• [Presentation] Measurement and comparison of V(V)-reductase activity of Vanabin family of a vanadium-rich ascidian Ascidia sydneiensis samea (2010)
  • Author(s): 植木龍也
  • Organizer: 第7回国際バナジウム化学・生物学シンポジウム
  • Place of Presentation: 富山市
  • Year and Date: 2010-10-08
• [Presentation] Identification of genes regulated by excess vanadium ions in ascidians, focusing on redox and accumulation of metals (2010)
  • Author(s): 植木龍也
  • Organizer: 第7回国際バナジウム化学・生物学シンポジウム
  • Place of Presentation: 富山市
  • Year and Date: 2010-10-08
• [Presentation] トランスポゾン挿入ミュータジェネシスによるバナジウム濃縮に関連するホヤ変異体の探索 (2010)
  • Author(s): 植木龍也
  • Organizer: 中国・四国動物生理シンポジウム
  • Place of Presentation: 土佐市
  • Year and Date: 2010-09-03
• [Presentation] バナジウム結合タンパク質Vanabinファミリーの五価バナジウム還元能の解析 (2010)
  • Author(s): 植木龍也
  • Organizer: 中国・四国動物生理シンポジウム
  • Place of Presentation: 土佐市
  • Year and Date: 2010-09-03
• [Presentation] バナジウムによって発現制御されるカタユウレイボヤ遺伝子の解析、特にグルタチオン代謝の変化について (2010)
  • Author(s): 植木龍也
  • Organizer: 第24回海洋生物活性談話会
  • Place of Presentation: 竹原市
  • Year and Date: 2010-06-05
• [Presentation] Expressed Sequence Tag (EST) Analysis of Intestine from the most Vanadium-rich Ascidian, Ascidia gemmata, and the Identification of Ag Vanabins. (2010)
  • Author(s): 植木龍也
  • Organizer: 第24回海洋生物活性談話会
  • Place of Presentation: 竹原市
  • Year and Date: 2010-06-05
• [Presentation] スジキレボヤから単離したバナジウム輸送体Nrampの機能解析 (2010)
  • Author(s): 植木龍也
  • Organizer: 第24回海洋生物活性談話会
  • Place of Presentation: 竹原市
  • Year and Date: 2010-06-05
• [Presentation] カタユウレイボヤにおいてバナジウム添加で発現が制御される遺伝子の解析 (2010)
  • Author(s): 植木龍也
  • Organizer: 生物系三学会中国四国支部大会
  • Place of Presentation: 山口市
  • Year and Date: 2010-05-15
• [Presentation] バナジウム暴露によるMRP(multidrug resistance protein)遺伝子の発現変動 (2010)
  • Author(s): 植木龍也
  • Organizer: 生物系三学会中国四国支部大会
  • Place of Presentation: 山口市
  • Year and Date: 2010-05-15
• [Presentation] スジキレボヤ血球細胞におけるチオレドキシン系酵素群の遺伝子クローニング (2010)
  • Author(s): 植木龍也
  • Organizer: 生物系三学会中国四国支部大会
  • Place of Presentation: 山口市
  • Year and Date: 2010-05-15
• [Presentation] Nramp ファミリーの新規バナジウム/プロトン共役輸送体 (2009)
  • Author(s): 植木龍也
  • Organizer: 日本生化学会第82回大会
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
  • Year and Date: 2009-10-21
• [Presentation] バナジウム添加で発現制御されるカタユウレイボヤの酸化還元遺伝子 (2009)
  • Author(s): 久米悟士
  • Organizer: 日本生化学会第82回大会
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
  • Year and Date: 2009-10-21
• [Presentation] バナジウム結合タンパク質VBP-129におけるバナジウム結合部位 (2009)
  • Author(s): 中川貴文
  • Organizer: 日本生化学会第82回大会
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
  • Year and Date: 2009-10-21
• [Presentation] スジキレボヤ由来の Nramp による四価バナジウム輸送 (2009)
  • Author(s): 植木龍也
  • Organizer: 日本動物学会第80回大会
  • Place of Presentation: グランシップ(静岡県)
  • Year and Date: 2009-09-17
• [Presentation] A novel vanadium reductase, Vanabin2, forms a possible cascade involved in electron transfer. (2009)
  • Author(s): Michibata, H., Ueki, T.
  • Organizer: 第14回国際生物無機化学会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-07-25
• [Presentation] A novel vanadium transporter of the Nramp/DCT family from a vanadium-rich ascidian Ascidia sydneiensis samea. (2009)
  • Author(s): Ueki, T., Michibata, H.
  • Organizer: 第14回国際生物無機化学会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-07-25
• [Presentation] A Novel Vanadium Reductase, Vanabin2, Forms a Possible Cascade Involved in Electron Transfer (2009)
  • Author(s): 道端齊
  • Organizer: 第14回国際生物無機化学会
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2009-07-25
• [Presentation] A Novel Vanadium Transporter of the Nramp/DCT Family from a Vanadium-Rich Ascidian Ascidia sydneiensis samea (2009)
  • Author(s): 植木龍也
  • Organizer: 第14回国際生物無機化学会
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2009-07-25
• [Presentation] スジキレボヤ血球における硫酸イオンの役割と膜輸送体 (2009)
  • Author(s): 植木龍也
  • Organizer: 第23回海洋生物活性談話会
  • Place of Presentation: 三崎臨海実験所(神奈川県)
  • Year and Date: 2009-05-23
• [Presentation] バナジウム結合タンパク質 Vanabin ファミリーによるバナジウム還元能の解析 (2009)
  • Author(s): 植木龍也
  • Organizer: 生物系三学会大会中国四国支部・高知大会
  • Place of Presentation: 高知大学(高知県)
  • Year and Date: 2009-05-16
• [Presentation] バナジウム暴露で発現が制御されるカタユウレイボヤの遺伝子解析 (2009)
  • Author(s): 久米悟士
  • Organizer: 生物系三学会大会中国四国支部・高知大会
  • Place of Presentation: 高知大学(高知県)
  • Year and Date: 2009-05-16
• [Presentation] バナジウム結合タンパク質VBP-129の金属選択性に関する解析 (2009)
  • Author(s): 中川貴文
  • Organizer: 生物系三学会大会中国四国支部・高知大会
  • Place of Presentation: 高知大学(高知県)
  • Year and Date: 2009-05-16
• [Presentation] バナジウム結合タンパク質の構造及び発現と相互作用 (2008)
  • Author(s): 植木 龍也
  • Organizer: 生物物理学会第46回年会
  • Place of Presentation: 福岡市
  • Year and Date: 2008-12-03
• [Presentation] スジキレボヤ血球のバナジウム結合タンパク質VBP-129の金属選択性に関する研究 (2008)
  • Author(s): 中川貴文, 植木 龍也, 他
  • Organizer: 平成20年度動物生理シンポジウム
  • Place of Presentation: 竹原市
  • Year and Date: 2008-09-19
• [Presentation] スジキレボヤとヒトの金属輸送体Metal ATPaseの金属選択性に関する研究 (2008)
  • Author(s): 田邉 哲也, 植木 龍也, 他
  • Organizer: 平成20年度動物生理シンポジウム
  • Place of Presentation: 竹原市
  • Year and Date: 2008-09-19
• [Presentation] スジキレボヤ血球で発現するVanabin2バリアントの解析 (2008)
  • Author(s): 植木 龍也
  • Organizer: 日本動物学会第79回大会
  • Place of Presentation: 福岡市
  • Year and Date: 2008-09-05
• [Presentation] Protein-protein interactions among vanadium-binding proteins and related proteins in a vanadium-rich ascidian. (2008)
  • Author(s): Ueki, T., Michibata, H.
  • Organizer: 第6回国際バナジウムシンポジウム
  • Place of Presentation: リスボン(ポルトガル)
  • Year and Date: 2008-07-17
• [Presentation] Protein-protein interactions among vanadium-binding proteins and related proteins in a vanadium-rich ascidian (2008)
  • Author(s): 植木 龍也
  • Organizer: 第6回国際バナジウムシンポジウム
  • Place of Presentation: リスボン, ポルトガル
  • Year and Date: 2008-07-17
• [Presentation] A novel function of Vanabin2 and the relationship among proteins involved in the accumulation and reduction of vanadium by ascidians (2008)
  • Author(s): 道端 齊, 植木 龍也, 他
  • Organizer: 第6回国際バナジウムシンポジウム
  • Place of Presentation: リスボン, ポルトガル
  • Year and Date: 2008-07-17
• [Presentation] Vanabin2 extracted from ascidian vanadocyte is a novel vanadium reductase (2008)
  • Author(s): 川上 了史, 植木 龍也, 他
  • Organizer: 第6回国際バナジウムシンポジウム
  • Place of Presentation: リスボン, ポルトガル
  • Year and Date: 2008-07-17
• [Presentation] スジキレボヤ血球からクローニングした硫酸イオン輸送体AsSUL1の解析 (2008)
  • Author(s): 植木 龍也, 他
  • Organizer: 平成20年度日本動物学会中国四国支部大会
  • Place of Presentation: 東広島市
  • Year and Date: 2008-05-17
• [Presentation] バナジウム添加に応答するカタユウレイボヤの遺伝子発現解析 (2008)
  • Author(s): 久米 悟士, 植木 龍也, 他
  • Organizer: 平成20年度日本動物学会中国四国支部大会
  • Place of Presentation: 東広島市
  • Year and Date: 2008-05-17
• [Presentation] スジキレボヤ血球細胞におけるチオレドキシン関連遺伝子のクローニング (2008)
  • Author(s): 矢野 克佳, 植木 龍也, 他
  • Organizer: 平成20年度日本動物学会中国四国支部大会
  • Place of Presentation: 東広島市
  • Year and Date: 2008-05-17
• [Book] Vanadium compounds/vanadate oligomers in biological systems : Chemistry, biochemistry and biological effects Research Signpost Inc (2008)
  • Author(s): Ueki, T., Michibata, H.
  • Total Pages: 356
• [Book] Vanadium compounds/vanadate oligomers in biological systems : Chemistry, biochemistry and biological effects (2008)
  • Author(s): 植木 龍也, 他
  • Total Pages: 356
  • Publisher: Research Signpost Inc
• [Remarks] ホームページ等
  • URL: http://home.hiroshima-u.ac.jp/bio/APN/index.html