| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
Ca^<2+> is transported by sarcoplasmic reticulm Ca^<2+>-pump through formation and hydrolysis of phosphoenzyme intermediate (EP) phosphorylated with ATP. Ca^<2+> ions bound to EP from cytoplasm are released into lumen in its isomerization (E1P・Ca_2→E2P). I found structural elements essential for the isomerization, and found a new EP intermediate (E2P・Ca_2) between these two EPs. To perform atomic or other structural analysis, I then developed stable analogs for E1P・Ca_2 and E2P・Ca_2. Furthermore, I have elucidated some new roles of the K+ ion bound to EP during Ca^<2+> transport and the mechanism of pump activation by high-concentration of ATP.
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