Interaction analysis of chromosomal replication related proteins in Escherichia coli.

• Project/Area Number: 20570110
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: Kyushu University
• Principal Investigator: ABE Yoshito Kyushu University, 薬学研究院, 准教授 (60315091)
• Co-Investigator(Kenkyū-buntansha): KATAYAMA Tsutomu 九州大学, 薬学研究院, 教授 (70264059)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 分子認識および相互作用 / 複製 / NMR / DNA複製 / 蛋白質構造 / 蛋白質間相互作用 / 蛋白質複合体

Research Abstract

Most of functional proteins work in protein complex and interact with other proteins. In this study, we examined protein-protein interaction based on the protein structure using NMR and amino acid mutation analysis. We found and discussed the function of CedA, HSPQ, PriB, PriC, and DnaT involved in replication in Escherichia coli.

Research Products

• [Journal Article] Evidence for the binding of phosphate ion to the C-terminus region in A β 1-40 using heteronuclear NMR analyses (2010)
  • Author(s): Nagata-Uchiyama M, Abe Y, Monji A, Kanba S, Ueda T
  • Journal Title: Protein Peptide lett. 17 Pages: 176-180
  • Peer Reviewed
• [Journal Article] Effect of protein concentration and pH on the chitinase activity of Tapes japonica lysozyme (2010)
  • Author(s): Goto T, Abe Y, Imoto T, Ueda T
  • Journal Title: Protein Peptide lett. 17 Pages: 172-175
  • Peer Reviewed
• [Journal Article] Structure of membrane domain of human erythrocyte anion exchanger 1 revealed by electron crystallography (2010)
  • Author(s): Yamaguchi T, Ikeda Y, Abe Y, Kuma H, Kang D, Hamasaki N, Hirai T
  • Journal Title: J. Mol. Biol. 397,1 Pages: 179-189
  • Peer Reviewed
• [Journal Article] Mutation of His 834 in human anion exchanger 1 affects substrate binding (2010)
  • Author(s): Takazaki S, Abe Y, Yamaguchi T, Yagi M, Ueda T, Kang D, Hamasaki N
  • Journal Title: BBA -Biomembranes 1798,5 Pages: 903-908
  • Peer Reviewed
• [Journal Article] Evaluation of the conformational equilibrium of reduced hen egg lysozyme by antibodies to the native form (2010)
  • Author(s): Oda M, Kitai A, Murakami A, Nishimura M, Ohkuri T, Abe Y, Ueda T, Nakamura H, Azuma T
  • Journal Title: Arch Biochem Biophys 494,2 Pages: 145-150
  • Peer Reviewed
• [Journal Article] Helical image reconstruction of the outward-open human erythrocyte band 3 membrane domain in tubular crystals (2010)
  • Author(s): Yamaguchi T, Fujii T, Abe Y, Hirai T, Kang D, Namba K, Hamasaki N, Mitsuoka K
  • Journal Title: J. Struc. Biol. 169,3 Pages: 406-412
  • Peer Reviewed
• [Journal Article] Effects of His mutations on the fibrillation of amyloidogenic Vλ 6 protein Wil under acidic and physiological conditions (2010)
  • Author(s): Mishima T, Ohkuri T, Monji A, Kanemaru T, Abe Y, Ueda T
  • Journal Title: Biochem Biophys Res Commun 391,1 Pages: 615-620
  • Peer Reviewed
• [Journal Article] Helical image reconstruction of the outward-open human erythrocyte band 3 membrane domain in tubular crystals (2010)
  • Author(s): Yamaguchi, et al.
  • Journal Title: Journal of structural biology 169 Pages: 406-412
  • Peer Reviewed
• [Journal Article] Evaluation of the conformational equilibrium of reduced hen egglysozyme by antibodies to the native form (2010)
  • Author(s): Oda, et al.
  • Journal Title: Archives of biochemistry and biophysics 494 Pages: 145-150
  • Peer Reviewed
• [Journal Article] Effects of His mutations on the fibrillation of amyloidogenic Vλ6 protein Wil under acidic and physiological conditions (2010)
  • Author(s): Mishima, et al.
  • Journal Title: Biochemical and biophysical research communication 391 Pages: 615-620
  • Peer Reviewed
• [Journal Article] Structure of membrane domain of human erythrocyte anion exchanger 1 revealed by electron crystallography (2010)
  • Author(s): Yamaguchi, et al.
  • Journal Title: Journal of molecular biology 397 Pages: 179-189
  • Peer Reviewed
• [Journal Article] Mutation of His 834 in human anion exchanger 1 affects substrate binding (2010)
  • Author(s): Takazaki, et al.
  • Journal Title: BBA- biomembrane 1710 Pages: 903-968
  • Peer Reviewed
• [Journal Article] Evidence for the binding of phosphate ion to the C-terminus region in Ass1-40 using heteronuclear NMR analyses (2010)
  • Author(s): Nagata-Uchiyama, et al.
  • Journal Title: Protein and peptide letters 17 Pages: 172-175
  • Peer Reviewed
• [Journal Article] Effect of protein concentration and pH on the chitinase activity of Tapes japonica lysozyme (2010)
  • Author(s): Goto, et al.
  • Journal Title: Protein and peptide letters 17 Pages: 176-180
  • Peer Reviewed
• [Journal Article] Residual Structures in the Acid-Unfolded States of Vlambda6 Proteins Affect Amyloid Fibrillation. (2009)
  • Author(s): Mishima T, Ohkuri T, Monji A, Kanemaru T, Abe Y, Ueda T
  • Journal Title: J. Mol. Biol. 392 Pages: 1033-1043
  • Peer Reviewed
• [Journal Article] DiaA dynamics are coupled with changes in initial origin complexes leading to helicase loading. (2009)
  • Author(s): Keyamura K, Abe Y, Higashi M, Ueda T, Katayama T
  • Journal Title: J. Biol. Chem. 284 Pages: 25038-25050
  • Peer Reviewed
• [Journal Article] Factor G Utilizes a Carbohydrate-Binding Cleft That Is Conserved between Horseshoe Crab and Bacteria for the Recognition of {β}-1,3-d-Glucans. (2009)
  • Author(s): Ueda Y, Ohwada S, Abe Y, Shibata T, Iijima M, Yoshimitsu Y, Koshiba T, Nakata M, Ueda T, Kawabata SI.
  • Journal Title: J. Immunol. 183 Pages: 3810-3818
  • Peer Reviewed
• [Journal Article] アサリ貝リゾチームの構造と機能 (2009)
  • Author(s): 阿部義人, 植田正
  • Journal Title: 生化学 81 Pages: 314-319
  • NAID: 10024916502
  • Peer Reviewed
• [Journal Article] DiaA dynamics are coupled with changes in initial origin complexes leading to helicase loading (2009)
  • Author(s): Keyamura, et al
  • Journal Title: Journal of biological chemistry 284 Pages: 25038-25050
  • Peer Reviewed
• [Journal Article] Residual Structures in the Acid-Unfolded States of Vlambda6 Proteins Affect Amyloid Fibrillation (2009)
  • Author(s): Mishima, et al
  • Journal Title: Journal of molecular biology 392 Pages: 1033-1043
  • Peer Reviewed
• [Journal Article] Factor G Utilizes a Carbohydrate-Binding Cleft That Is Conserved between Horseshoe Crab and Bacteria for the Recognition of {beta}-1, 3-d-Glucans (2009)
  • Author(s): Ueda, et al.
  • Journal Title: Journal of immunology 183 Pages: 3810-3818
  • Peer Reviewed
• [Journal Article] アサリ貝リゾチームの構造と機能 (2009)
  • Author(s): 阿部義人, 他
  • Journal Title: 生化学 81 Pages: 314-319
  • NAID: 10024916502
• [Journal Article] アサリ貝リゾチームの構造と機能 (2009)
  • Author(s): 阿部 義人, 他
  • Journal Title: 生化学 (In press)
  • NAID: 10024916502
• [Journal Article] Crystal structures of K33 mutant hen lysozymes with enhanced activities. (2008)
  • Author(s): Goto T, Ohkuri T, Shioi S, Abe Y, Imoto T, Ueda T
  • Journal Title: J. Biochem. 144 Pages: 619-623
  • NAID: 10024913411
  • Peer Reviewed
• [Journal Article] Crystal structures of K33 mutant hen lysozymes with enhanced activities. (2008)
  • Author(s): Goto, et al.
  • Journal Title: J. Biochemistry 144 Pages: 619-623
  • Peer Reviewed
• [Presentation] 大腸菌の複製再開始蛋白質の構造と-本鎖DNA認識 (2011)
  • Author(s): 阿部義人, 他
  • Organizer: 平成23年度 九大P&P拡大研究会「超高次複合体解析に基づくゲノム動態研究プロジェクト」
  • Place of Presentation: 福岡
  • Year and Date: 2011-01-12
• [Presentation] 大腸菌の複製再開始蛋白質の構造と一本鎖DNA認識 (2011)
  • Author(s): 阿部義人、片山勉、植田正
  • Organizer: 平成23年度九大P&P拡大研究会「超高次複合体解析に基づくゲノム動態研究プロジェクト」
• [Presentation] DNA複製再開始におけるプライモソーム構成因子PriCの構造機能解析 (2010)
  • Author(s): 荒牧峻彦, 他
  • Organizer: 第33回日本分子生物学会年会 第83回日本生化学会大会 合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-07
• [Presentation] NMRを用いた大腸菌複製再開始因子PriBの相互作用解析 (2010)
  • Author(s): 阿部義人, 他
  • Organizer: 第10回日本タンパク質科学会
  • Place of Presentation: 札幌
  • Year and Date: 2010-06-16
• [Presentation] 大腸菌DNA複製再開始因子PriCのドメイン化およびその構造と機能 (2010)
  • Author(s): 荒牧峻彦, 他
  • Organizer: 第10回日本タンパク質科学会
  • Place of Presentation: 札幌
  • Year and Date: 2010-06-16
• [Presentation] DNA複製再開始におけるプライモソーム構成因子PriCの構造機能解析 (2010)
  • Author(s): 荒牧峻彦、阿部義人、大栗誉敏、植田正
  • Organizer: 第33回日本分子生物学会年会 第83回日本生化学会大会 合同大会
• [Presentation] NMRを用いた大腸菌複製再開始因子PriBの相互作用解析 (2010)
  • Author(s): 阿部義人、竹縄太一、塩井誠次郎、片山勉、植田正
  • Organizer: 第10回日本蛋白質科学会
• [Presentation] 大腸菌DNA複製再開始因子PriCのドメイン化およびその構造と機能 (2010)
  • Author(s): 荒牧峻彦、阿部義人、大栗誉敏、三島朋徳、片山勉、植田正
  • Organizer: 第10回日本蛋白質科学会
• [Presentation] 大腸菌DNA複製再開始因子PriCのドメイン化およびその構造と機能について (2009)
  • Author(s): 荒牧峻彦, 他
  • Organizer: P&P合同公開シンポジウム「生命活動を制御する高次複合体の構造と機能」
  • Place of Presentation: 福岡
  • Year and Date: 2009-12-23
• [Presentation] 構造生物学アプローチによる大腸菌細胞分裂阻害再活性化因子CedAの機能解析 (2009)
  • Author(s): 阿部義人, 他
  • Organizer: P&P合同公開シンポジウム「生命活動を制御する高次複合体の構造と機能」
  • Place of Presentation: 福岡
  • Year and Date: 2009-12-23
• [Presentation] 大腸菌熱ショック蛋白質HSPQの機能解析 (2009)
  • Author(s): 中田光, 他
  • Organizer: 蛋白質科学会
  • Place of Presentation: 熊本
  • Year and Date: 2009-12-23
• [Presentation] 大腸菌プライモソーム構成因子PriC N末ドメインのNMR構造解析 (2009)
  • Author(s): 荒牧峻彦, 他
  • Organizer: 日本生化学会九州支部会
  • Place of Presentation: 福岡
  • Year and Date: 2009-05-16
• [Presentation] DNA複製再開に関わるプライモソーム構成因子 DnaT のドメイン化及びNMRに よる構造解析 (2009)
  • Author(s): 谷純也, 他
  • Organizer: 日本生化学会九州支部会
  • Place of Presentation: 福岡
  • Year and Date: 2009-05-16
• [Presentation] 構造生物学アプローチによる大腸菌細胞分裂阻害再活性化因子CedAの機能解析 (2009)
  • Author(s): 阿部義人、三好孝法、渡邊典子、片山勉、植田正
  • Organizer: P&P合同公開シンポジウム「生命活動を制御する高次複合体の構造と機能」
• [Presentation] 大腸菌DNA複製再開始因子PriCのドメイン化およびその構造と機能について (2009)
  • Author(s): 荒牧峻彦、阿部義人、大栗誉敏、三島朋徳、片山勉、植田正
  • Organizer: P&P合同公開シンポジウム「生命活動を制御する高次複合体の構造と機能」
• [Presentation] 大腸菌熱ショック蛋白質HSPQの機能解析 (2009)
  • Author(s): 中田光、阿部義人、塩井誠次郎、片山勉、植田正
  • Organizer: 第9回日本蛋白質科学会
• [Presentation] DNA複製再開に関わるプライモソーム構成因子DnaTのドメイン化及びNMRによる構造解析 (2009)
  • Author(s): 谷純也、阿部義人、佐藤健治、片山勉、植田正
  • Organizer: 日本生化学会九州支部会
• [Presentation] 大腸菌プライモソーム構成因子PriC N末ドメインのNMR構造解析 (2009)
  • Author(s): 荒牧峻彦, 阿部義人, 大栗誉敏, 三島朋徳, 片山勉
  • Organizer: 日本生化学会九州支部会
• [Presentation] 細胞分裂阻害再活性化因子CedAの機能解析 (2008)
  • Author(s): 三好孝法, 他
  • Organizer: 日本分子生物学会・生化学会合同年会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12
• [Presentation] 大腸菌熱ショックタンパク質HSPQの機能解析 (2008)
  • Author(s): 中田光, 他
  • Organizer: 日本薬学会九州支部大会
  • Place of Presentation: 宮崎
  • Year and Date: 2008-12-07
• [Presentation] ラットATP受容体細胞外ドメインの大腸菌発現系構築 (2008)
  • Author(s): 東 貞行, 他
  • Organizer: 日本薬学会九州支部大会
  • Place of Presentation: 宮崎
  • Year and Date: 2008-12-06
• [Presentation] 変性条件下における抗体λ6鎖の残存構造がアミロイド繊維形成に及ぼす影響 (2008)
  • Author(s): 三島朋徳, 他
  • Organizer: 日本生物物理学会
  • Place of Presentation: 福岡
  • Year and Date: 2008-12-05
• [Presentation] ヒト赤血球膜蛋白質バンド3膜貫通ドメインの電子線結晶構造解析 (2008)
  • Author(s): 山口知宏, 他
  • Organizer: 日本生物物理学会
  • Place of Presentation: 福岡
  • Year and Date: 2008-12-05
• [Presentation] Effect of the phosphate ion on the A-beta amyloidogenesis using heteronuclear NMR (2008)
  • Author(s): Uchiyama M, et al.
  • Organizer: 20th FAOBMB Taipei Conference
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2008-10-24
• [Presentation] ヒト赤血球膜蛋白質Band3膜貫通ドメインの極低温電子顕微鏡構造解析 (2008)
  • Author(s): 山口知宏, 他
  • Organizer: 日本顕微鏡学会
  • Place of Presentation: 京都
  • Year and Date: 2008-05-23
• [Presentation] 構造生物学的アプローチによる大腸菌複製開始因子DnaAの機能解析 (2008)
  • Author(s): 阿部義人, 他
  • Organizer: 日本生化学会九州支部例会
  • Place of Presentation: 福岡
  • Year and Date: 2008-05-18
• [Presentation] 細胞分裂阻害再活性化因子CedAの機能解析 (2008)
  • Author(s): 三好孝法、阿部義人、渡辺典子、片山勉、植田正
  • Organizer: 第31日本分子生物学会・第83回生化学会合同年会
• [Presentation] 大腸菌熱ショックタンパク質HSPQの機能解析 (2008)
  • Author(s): 中田光、阿部義人、塩井誠次郎、片山勉、植田正
  • Organizer: 日本薬学会九州支部大会
• [Presentation] 構造生物学的アプローチによる大腸菌複製開始因子DnaAの機能解析 (2008)
  • Author(s): 阿部義人、大栗誉敏、片山勉、植田正
  • Organizer: 日本生化学会九州支部例会
• [Remarks] ホームページ等