Molecular mechanism of genome maintenance in Thermus thermophilus
| Project/Area Number |
20570131
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Osaka University |
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Principal Investigator |
MASUI Ryoji Osaka University, 大学院・理学研究科, 准教授 (40252580)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 酵素 / 核酸 / 生体分子 / ゲノム / 細菌 / 塩基除去修復 / ミスマッチ修復 / DNAポリメラーゼ / 立体構造 / 高度好熱菌 / 組換え修復 / RecJ / APエンドヌクレアーゼ / MutS / MutL / ウラシルDNAグリコシラーゼ / DNAポリメラーゼX |
|---|
| Research Abstract |
I prepared Thermus thermophilus HB8 proteins involved in mismatch repair and base excision repair systems and performed reconstitution of repair reactions, protein-protein interaction analysis and crystal structural analyss. Based on the results, I revealed that novel interactions between mismatch repair proteins contribute to strand discrimination, the nuclease which degrades an error-strand recognizes single-straded DNA by its unique structure, and two DNA polymerases function in different pathways of base excision repair.
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Report
(4 results)
Research Products
(77 results)
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[Journal Article] Long type endonuclease IV has an additional C-terminal loop that increases binding affinity to DNA.2011
Author(s)
Asano, R., Ishikawa, H., Nakane, S., Nakagawa, N., Kuramitsu, S., Masui, R.
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Journal Title
Acta Crystallogr. D67
Pages: 149-155
Related Report
Peer Reviewed
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[Journal Article] A novel single-stranded DNA-specific 3'-5' exonuclease, Thermus thermophilus exonuclease I, is involved in several DNA repair pathways.2010
Author(s)
Shimada, A., Masui, R., Nakagawa, N., Takahata, Y., Kim, K., Kuramitsu, S., Fukui, K.
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Journal Title
Nucleic Acids Res. 38
Pages: 5692-5705
Related Report
Peer Reviewed
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[Journal Article] Structure of RecJ exonuclease defines its specificity for single-stranded DNA.2010
Author(s)
Wakamatsu, T., Kitamura, Y., Kotera, Y., Nakagawa, N., Kuramitsu, S., Masui, R.
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Journal Title
J.Biol.Chem. 285
Pages: 9762-9769
Related Report
Peer Reviewed
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[Journal Article]2009
Author(s)
倉光成紀
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Journal Title
やさしい原理からはいるタンバク質科学実験法3 : タンパク質のはたらきを知る(化学同人)
Pages: 1-21
Related Report
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