Functions of telomere in chromosome segregation

• Project/Area Number: 20570165
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Molecular biology
• Research Institution: Osaka University (2009-2010); Kyoto University (2008)
• Principal Investigator: KANOH Junko 大阪大学, 蛋白質研究所, 特任准教授 (10323809)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: テロメア / 細胞分裂 / 染色体 / リン酸化 / 核膜 / Cdc2

Research Abstract

To investigate the functions of telomere in mitosis, we analyzed the regulatory mechanisms of telomere in fission yeast, which undergoes closed mitosis without the nuclear envelope breakdown. Fission yeast telomeres were anchored to the NE in interphase, whereas they were transiently released from the NE in M phase, which was important for the chromosome stability. Furthermore, the Rap1 protein, one of the central telomere proteins, was phosphorylated in mitosis, which induced the detachment of Rap1 from a nuclear membrane protein Bqt4. We proposed the model that the detachment of telomeres from the NE is a common mechanism for both closed and open mitoses.

Research Products

• [Journal Article] A conserved motif within RAP1 plays diversified roles in telomere protection and regulation in different organisms (2011)
  • Author(s): Chen, Y., Rai, R., Zhou, Z-R., Kanoh, J., Ribeyre, C., Yang, Y., Zheng, H., Damay, P., Wang, F., Tsujii, H., Hiraoka, Y., Shore, D., Hu, H-Y., Chang, S. and Lei, M.
  • Journal Title: Nature Struct. Mol. Biol. Volume: 18 Pages: 213-221
  • Peer Reviewed
• [Journal Article] A conserved motif within RAP1 has diversified roles in telomere protection and regulation in different organisms (2011)
  • Author(s): Chen, Y., Kanoh, J., et al
  • Journal Title: Nature Structural and Molecular Biology Volume: VOL18 Pages: 213-221
  • Peer Reviewed
• [Journal Article] Tel2蛋白質によるPIKKファミリー蛋白質の制御機構 (2011)
  • Author(s): 加納純子
  • Journal Title: 細胞周期フロンティア Pages: 119-124
• [Journal Article] Fission yeast Vps1 and Atg8 contribute to oxidative stress resistance (2010)
  • Author(s): Mikawa, T., Kanoh, J. and Ishikawa, F.
  • Journal Title: Genes Cells Volume: 15 Pages: 229-242
  • Peer Reviewed
• [Journal Article] Structure of TOR complexes in fission yeast (2010)
  • Author(s): Kanoh, J. and Yanagida, M.
  • Journal Title: Enzymes Volume: 27 Pages: 271-284
  • Peer Reviewed
• [Journal Article] Structure of TOR complexes in fission yeast (2010)
  • Author(s): Kanoh, J., Yanagida, M.
  • Journal Title: Enzymes Volume: VOL27 Pages: 271-284
• [Journal Article] Fission yeast Vps1 and Atg8 contribute to oxidative stress resistance (2010)
  • Author(s): Mikawa T., Kanoh J., Ishikawa F.
  • Journal Title: Genes to Cells 15 Pages: 229-242
  • Peer Reviewed
• [Journal Article] PIKKファミリータンパク質に結合するTel2 (2010)
  • Author(s): 加納純子
  • Journal Title: 生化学 82 Pages: 57-60
  • NAID: 10026300313
• [Journal Article] Fission yeast Ku protein is required for recovery from DNA replication stress (2009)
  • Author(s): Miyoshi, T., Kanoh, J. and Ishikawa, F.
  • Journal Title: Genes Cells Volume: 14 Pages: 1091-1103
  • Peer Reviewed
• [Journal Article] Fission yeast Ku protein is required for recovery from DNA replication stress (2009)
  • Author(s): Miyoshi T., Kanoh J., Ishikawa F.
  • Journal Title: Genes to Cells 14 Pages: 1091-1103
  • Peer Reviewed
• [Journal Article] テロメア特異的なクロマチン構造によるテロメアDNA維持機構 (2009)
  • Author(s): 加納純子
  • Journal Title: 蛋白質核酸酵素(増刊「染色体サイクル」) 54 Pages: 514-520
• [Journal Article] Fission yeast Potl-Tppl protects telomeres and regulates telomere length. (2008)
  • Author(s): Miyoshi T., Kanoh J., Saito M., Ishikawa F.
  • Journal Title: Science 320 Pages: 1341-1344
  • Peer Reviewed
• [Presentation] Roles of telomere in closed mitosis (2011)
  • Author(s): 加納純子
  • Organizer: 第6回分裂酵母国際会議
  • Place of Presentation: ハーバード大学(Boston, USA)(招待講演)
  • Year and Date: 2011-06-29
• [Presentation] Roles of telomere in closed mitosis (2011)
  • Author(s): Tsujii, H., Tanaka, M., Chikashige, Y., Watanabe, Y., Saito, M., Ishikawa, F., Hioraka, Y., and Kanoh, J.
  • Organizer: The sixth international fission yeast meeting
  • Place of Presentation: Boston, USA
  • Year and Date: 2011-05-29
• [Presentation] M phase-specific regulations of telomere (2011)
  • Author(s): 加納純子
  • Organizer: International symposium on physicochemical field for genetic activities
  • Place of Presentation: 淡路夢舞台(兵庫県)(招待講演)
  • Year and Date: 2011-01-25
• [Presentation] M phase-specific regulations if telomere (2011)
  • Author(s): Kanoh, J.
  • Organizer: International symposium on physicochemical filed for genetic activities
  • Place of Presentation: Awaji, Hyogo, Japan
  • Year and Date: 2011-01-24
• [Presentation] 細胞分裂期においてテロメア-核膜ネットワークが果たす機能 (2011)
  • Author(s): 辻井久代、近重裕次、田中麻紀子、渡邊祐三、齋藤基輝、石川冬木、平岡泰、加納純子
  • Organizer: 第28回染色体ワークショップ
  • Place of Presentation: 加賀、石川
  • Year and Date: 2011-01-11
• [Presentation] 細胞分裂期においてテロメア-核膜ネットワークが果たす機能 (2011)
  • Author(s): 加納純子
  • Organizer: 第28回染色体ワークショップ
  • Place of Presentation: 加賀山代温泉(石川県)
  • Year and Date: 2011-01-11
• [Presentation] 細胞分裂期におけるテロメアと核膜のネットワーク機能 (2010)
  • Author(s): 辻井久代、近重裕次、渡邊祐三、齋藤基輝、石川冬木、平岡泰、加納純子
  • Organizer: BMB2010
  • Place of Presentation: 神戸、兵庫、日本
  • Year and Date: 2010-12-07
• [Presentation] 細胞分裂期におけるテロメアと核膜のネットワーク機能 (2010)
  • Author(s): 加納純子
  • Organizer: BMB2010
  • Place of Presentation: 神戸ポートアイランド(兵庫県)(招待講演)
  • Year and Date: 2010-12-07
• [Presentation] Shelterin complex protects and regulates telomere DNA (2010)
  • Author(s): 加納純子
  • Organizer: 第7回日独先端科学シンポジウム
  • Place of Presentation: ポツダム(ドイツ)
  • Year and Date: 2010-11-12
• [Presentation] テロメア結合タンパク質Rap1を中心としたタンパク質相互作用ネットワークの分子基盤の解明 (2010)
  • Author(s): 加納純子
  • Organizer: 染色体サイクルの制御ネットワークシンポジウム
  • Place of Presentation: 東京大学(東京都)(招待講演)
  • Year and Date: 2010-10-01
• [Presentation] Regulations of telomere at M phase (2010)
  • Author(s): Tsujii, H., Chikashige, Y., Watanabe, Y., Saito, M., Ishikawa, F., Hiraoka, Y., and Kanoh, J.
  • Organizer: Telomeres and the DNA damage response
  • Place of Presentation: Marseille, France
  • Year and Date: 2010-09-16
• [Presentation] Regulations of telomere at M phase (2010)
  • Author(s): 加納純子
  • Organizer: EMBO conference : Telomeres and the DNA damage response
  • Place of Presentation: マルセイユ(フランス)(招待講演)
  • Year and Date: 2010-09-16
• [Presentation] テロメア.核膜ネットワークによるM期制御機構 (2010)
  • Author(s): 辻井久代、近重裕次、渡邊祐三、齋藤基輝、石川冬木、平岡泰、加納純子
  • Organizer: 第43回酵母遺伝学フォーラム
  • Place of Presentation: 奈良
  • Year and Date: 2010-09-09
• [Presentation] テロメア-核膜ネットワークによるM期制御機構 (2010)
  • Author(s): 加納純子
  • Organizer: 第43回酵母遺伝学フォーラム
  • Place of Presentation: ならまちセンター(奈良県)
  • Year and Date: 2010-09-09
• [Presentation] Regulations of telomere in cell division cycle (2010)
  • Author(s): Kanoh, J.
  • Organizer: The joint symposium of the 5^<th> international symposium of institute network
  • Place of Presentation: Kanazawa, Ishikawa, Japan
  • Year and Date: 2010-06-24
• [Presentation] Regulations of telomere in cell division cycle (2010)
  • Author(s): 加納純子
  • Organizer: 第5回研究所ネットワークシンポジウム
  • Place of Presentation: KKR金沢(石川県)(招待講演)
  • Year and Date: 2010-06-24
• [Presentation] 細胞分裂期における核膜とテロメアの機能 (2010)
  • Author(s): 辻井久代、近重裕次、渡邊祐三、齋藤基輝、石川冬木、平岡泰、加納純子
  • Organizer: 第9回核ダイナミクス研究会
  • Place of Presentation: 修善寺、静岡
  • Year and Date: 2010-05-29
• [Presentation] 細胞分裂期における核膜とテロメアの機能 (2010)
  • Author(s): 加納純子
  • Organizer: 第9回核ダイナミクス研究会
  • Place of Presentation: 修善寺(静岡県)
  • Year and Date: 2010-05-29
• [Presentation] REGULATIONS OF TELOMERES AT M PHASE (2009)
  • Author(s): Junko Kanoh
  • Organizer: Nasu Genome/Chromosome Dynamic meeting 2009
  • Place of Presentation: 栃木県那須郡那須町 ホテルサンバレー那須
  • Year and Date: 2009-11-22
• [Presentation] Regulations of telomeres at M phase (2009)
  • Author(s): Kanoh, J., Chikashige, Y., Watanabe, Y., Saito, M., Hiraoka, Y., and Ishikawa, F.
  • Organizer: The fifth international fission yeast meeting
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2009-10-27
• [Presentation] 細胞分裂期におけるテロメア結合タンパク質Rap1の制御機構 (2009)
  • Author(s): 加納純子、近重裕次、渡邊祐三、齋藤基輝、平岡泰、石川冬木
  • Organizer: 第42回酵母遺伝学フォーラム
  • Place of Presentation: つくば、茨城
  • Year and Date: 2009-07-28
• [Presentation] 細胞分裂期におけるテロメア結合因子Rap1の機能制御 (2008)
  • Author(s): 加納純子、石川冬木
  • Organizer: 第41回酵母遺伝学フォーラム
  • Place of Presentation: 札幌、北海道
  • Year and Date: 2008-09-11
• [Book] Tel2蛋白質によるPIKKファミリー蛋白質の制御機構,細胞周期フロンティア (2010)
  • Author(s): 加納純子
  • Publisher: 共立出版
• [Book] 「PIKKファミリータンパク質に結合するTel2」生化学 (2010)
  • Author(s): 加納純子
• [Book] 生物学辞典 (2010)
  • Author(s): 加納純子, 他
  • Publisher: 東京化学同人
• [Book] テロメア特異的なクロマチン構造によるテロメアDNA維持機構・蛋白質核酸酵素増刊・染色体サイクル (2009)
  • Author(s): 加納純子
  • Publisher: 共立出版
• [Remarks]
  • URL: http://www.protein.osaka-u.ac.jp/icr/network/
• [Remarks]
  • URL: http://www.protein.osaka-u.ac.jp/icr/network/