| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Research Abstract |
In order to elucidate the mechanisms of E.coli 100S ribosome formation, the structure of the 100S ribosome was observed by electron cryomicroscopy. The results revealed that the 100S ribosome is formed by two tRNA-free 70S ribosomes through the interactions between their 30S subunits and that the S2, S3 and S5 proteins are likely to be directly involved in the interactions. The functions of these proteins are related to the binding of mRNA to the ribosome. The undesirable binding of mRNA to the 100S ribosomes may be inhibited by the conformational changes around the S2-S5 region.
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