Analysis of RNA-binding proteins that regulate meiotic cell cycle in fission yeast

• Project/Area Number: 20570178
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cell biology
• Research Institution: The University of Tokyo
• Principal Investigator: YAMASHITA Akira The University of Tokyo, 大学院・理学系研究科, 講師 (30312276)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 細胞周期 / 減数分裂 / 発現制御 / RNA結合タンパク / 分裂酵母 / 分子生物学 / RNA結合タンパク質

Research Abstract

The aim of this study is to elucidate the function of RNA-binding proteins, Mei2p and Mmi1p, that regulate meiotic cell cycle in fission yeast. It turned out that phosphorylation of RNA polymerase II is crucial for the expression of Mei2p. It has been also proved that polyadenylation of meiotic mRNAs is essential for the selective elimination induced by Mmi1p.

Research Products

• [Journal Article] Importance of polyadenylation in the selective elimination of meiotic mRNAs in growing S. (2010)
  • Author(s): Yamanaka S., Yamashita A., Harigaya Y., Iwata R., Yamamoto M.
  • Journal Title: pombe cells.EMBO J. 29 Pages: 2173-2181
  • Peer Reviewed
• [Journal Article] Contribution of dynein light intermediate and intermediate chains to subcellular localization of the dynein-dynactin motor complex in Schizosaccharomyces pombe. (2010)
  • Author(s): Fujita I., Yamashita A., Yamoto M.
  • Journal Title: Genes to Cells 15 Pages: 359-372
  • Peer Reviewed
• [Journal Article] Importance of polyadenylation in the selective elimination of meiotic mRNAs in growing S. pombe cells (2010)
  • Author(s): Yamanaka, S., Yamashita, A., Harigaya, Y., Iwata, R., Yamamoto, M.
  • Journal Title: The EMBO Journal Volume: 29 Pages: 2173-2181
  • Peer Reviewed
• [Journal Article] Contribution of dynein light intermediate and intermediate chains to subcellular localization of the dynein-dynactin motor complex in Schizosaccharomyces pombe (2010)
  • Author(s): Ikumi Fujita, Akira Yamashita, Masayuki Yamamoto
  • Journal Title: Genes to Cells 15 Pages: 359-372
  • Peer Reviewed
• [Journal Article] 分裂酵母の減数分裂制御機構 (2008)
  • Author(s): 山下朗
  • Journal Title: 細胞工学 27 Pages: 486-490
  • NAID: 40016026342
• [Presentation] 分裂酵母の減数分裂特異的mRNAの選択的除去機構 (2010)
  • Author(s): 山下朗
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド(兵庫)
  • Year and Date: 2010-12-10
• [Presentation] 分裂酵母の減数分裂特異的mRNAの除去機構 (2009)
  • Author(s): 山下朗
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川)
  • Year and Date: 2009-12-12
• [Presentation] Molecular basis to eliminate meiotic transcripts from mitotic cells by an RNA-binding protein Mmilp (2009)
  • Author(s): Akira Yamashita
  • Organizer: The fifth international fission yeast meeting
  • Place of Presentation: 国立オリンピック記念青少年総合センター(東京)
  • Year and Date: 2009-10-29
• [Presentation] In vivo analysis of cytoplasmic dynein-dynactin complex in fission yeast (2009)
  • Author(s): Akira Yamashita
  • Organizer: Cytokinesis : dynamics of the contractile ring
  • Place of Presentation: 学習院大学(東京)
  • Year and Date: 2009-06-06
• [Presentation] 分裂酵母の減数分裂特異的mRNAの除去機構 (2008)
  • Author(s): 山下朗
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド(兵庫)
  • Year and Date: 2008-12-11
• [Presentation] 分裂酵母の減数分裂特異的mRNAの除去機構 (2008)
  • Author(s): 山下朗
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2008-12-11
• [Presentation] Analysis of meiotic mRNA removal induced by RNA-binding protein Mmilp (2008)
  • Author(s): Akira Yamashita
  • Organizer: Asia-Pacific regional S.pombe meeting
  • Place of Presentation: Genome Institute of Singapore (Singapore)
  • Year and Date: 2008-07-26
• [Presentation] Analysis of meiotic mRNA removal induced by RNA-binding protein Mmilp (2008)
  • Author(s): 山下朗
  • Organizer: Asia-Pacific regional S. pombe meeting
  • Place of Presentation: Genome Institute of Singapore
  • Year and Date: 2008-07-26
• [Presentation] 分裂酵母の減数分裂を制御するRNA結合タンパク質 (2008)
  • Author(s): 山下朗
  • Organizer: 第18回酵母合同シンポジウム
  • Place of Presentation: 甲南大学(兵庫)
  • Year and Date: 2008-06-05
• [Presentation] 分裂酵母の減数分裂を制御するRNA結合タンパク質 (2008)
  • Author(s): 山下朗
  • Organizer: 第18回酵母合同シンポジウム
  • Place of Presentation: 甲南大学甲友会館
  • Year and Date: 2008-06-05
• [Book] 細胞周期フロンティア(分裂酵母における減数分裂開始の分子機構) (2010)
  • Author(s): 山下朗
  • Publisher: 共立出版
• [Book] 細胞周期フロンティア(V章「分裂酵母における減数分裂開始の分子機構」) (2010)
  • Author(s): 山下朗
  • Publisher: 共立出版
• [Book] 細胞工学(分裂酵母の減数分裂制御機構) (27) (2008)
  • Author(s): 山下朗、山本正幸
  • Publisher: 秀潤社
• [Remarks] ホームページ等
  • URL: http://www.biochem.s.u-tokyo.ac.jp/yamamoto-lab/
• [Remarks]
  • URL: http://www.biochem.s.u-tokyo.ac.jp/yamamoto-lab/
• [Remarks]
  • URL: http://www.biochem.s.u-tokyo.ae.jp/yamamoto-lab/
• [Remarks]
  • URL: http://www.biochem.s.u-tokyo.ac.jp/yamamoto-lab/