Functional analysis of microRNAs on sex determination and differentiation

• Project/Area Number: 20570210
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Jichi Medical University
• Principal Investigator: TAKADA Shuji Jichi Medical University, ゲノム機能研究室, 室長 (20382856)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: マウス / microRNA / 生殖腺 / 性決定 / 性分化 / mRAP

Research Abstract

To examine if microRNA plays a role in gonad formation and sexual differentiation, microRNA profiles were determined by high-sensitive microRNA profiling method, mRAP, using mouse embryonic supporting cells purified from male and female gonads. The profiles obtained were further verified by TaqMan method. The results shows that mir-199a-5p is preferentially expressed in female supporting cells, suggesting that microRNA is associated with gonad formation and sexual differentiation in supporting cells in mouse embryonic gonad.

Research Products

• [Journal Article] Potential role of miR-29b in modulation of Dnmt3a and Dnmt3b expression in primordial germ cells of female mouse embryos. (2009)
  • Author(s): Takada S, Berezikov E, Choi YL, Yamashita Y, Mano H
  • Journal Title: RNA 15 Pages: 1507-1514
  • Peer Reviewed
• [Journal Article] Potential role of miR-29b in modulation of Dnmt3a and Dnmt3b expression in primordial germ cells of female mouse embryos (2009)
  • Author(s): Takada S, et al.
  • Journal Title: RNA 15 Pages: 1507-1514
  • Peer Reviewed
• [Presentation] 次世代シークェンサーとマイクロRNA研究 (2010)
  • Author(s): 高田修治
  • Organizer: 第11回運動器科学研究会
  • Place of Presentation: 万平ホテル軽井沢(長野県)
  • Year and Date: 2010-09-11
• [Presentation] 次世代シークエンサーとマイクロRNA研究. (2010)
  • Author(s): 高田修治
  • Organizer: 第11回運動器科学研究会 特別講演 最先端の研究技術による生物学シリーズ
  • Place of Presentation: 軽井沢
• [Presentation] Comprehensive analysis of microRNA expression. (2009)
  • Author(s): Shuji Takada, Yoshihiro Yamashita, Hiroshi Asahara, Hiroyuki Mano
  • Organizer: 第68回日本癌学会 Symposium on microRNA and cancer
  • Place of Presentation: 横浜
  • Year and Date: 2009-10-02
• [Presentation] microRNAの網羅的プロファイリング (2009)
  • Author(s): 高田修治, 他
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜(神奈川県横浜市)
  • Year and Date: 2009-10-02
• [Presentation] 雄のマウス胎児期生殖巣では始原生殖細胞でribosomal RNAからmicroRNAが生合成されている (2008)
  • Author(s): 高田修治
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] 雄のマウス胎児期生殖巣では始原生殖細胞でribosomal RNAからmicroRNAが生合成されている (2008)
  • Author(s): 高田修治、上野敏秀、間野博行
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会合同大会
  • Place of Presentation: 神戸