Functional analysis of microRNAs on sex determination and differentiation
| Project/Area Number |
20570210
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | Jichi Medical University |
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Principal Investigator |
TAKADA Shuji Jichi Medical University, ゲノム機能研究室, 室長 (20382856)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | マウス / microRNA / 生殖腺 / 性決定 / 性分化 / mRAP |
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| Research Abstract |
To examine if microRNA plays a role in gonad formation and sexual differentiation, microRNA profiles were determined by high-sensitive microRNA profiling method, mRAP, using mouse embryonic supporting cells purified from male and female gonads. The profiles obtained were further verified by TaqMan method. The results shows that mir-199a-5p is preferentially expressed in female supporting cells, suggesting that microRNA is associated with gonad formation and sexual differentiation in supporting cells in mouse embryonic gonad.
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Report
(4 results)
Research Products
(8 results)
