Molecular Phylogeny of Fornicata

• Project/Area Number: 20570219
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Evolutionary biology
• Research Institution: University of Tsukuba
• Principal Investigator: HASHIMOTO Tetsuo University of Tsukuba, 大学院・生命環境科学研究科, 教授 (50208451)
• Co-Investigator(Kenkyū-buntansha): INAGAKI Yuji 筑波大学, 計算科学研究センター, 准教授 (50387958)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 系統進化 / 真核生物 / 複数遺伝子分子系統解析 / フォルニケータ / カルペディエモナス様生物 / ミトコンドリア関連オルガネラ / Dysnectes brevis / Giardia intestinalis / 分子系統樹 / カルペデイエモナス様生物 / チューブリン / ペプチド鎖伸長因子 / HSP90

Research Abstract

To infer a robust phylogenetic tree of the group Fornicata, which comprises exclusively of amitochondriate flagellates, we sequenced several phylogenetic marker genes from free-living Fornicata organisms, and underwent multi-gene phylogeny. The tree finally obtained robustly reconstructed the relationships among subgroups of Fornicata. The tree could be a backbone for evolutionary comparison analyses of the mitochondrion-related organelles in Fornicata.

Research Products

• [Journal Article] Splintrons in Giardia intestinalis: Spliceosomal introns in a split form (2011)
  • Author(s): Kamikawa R, Inagaki Y, Roger AJ, Hashimoto T
  • Journal Title: Communicative and Integrative Biology (in press)
• [Journal Article] Split introns in the genome of a divergent eukaryote Giardia intestinalis are excised by spliceosome-mediated trans-splicing (2011)
  • Author(s): Kamikawa R, Inagaki Y, Tokoro M, Roger AJ, Hashimoto T
  • Journal Title: Current Biology 21(4) Pages: 311-315
  • Peer Reviewed
• [Journal Article] Split introns in the genome of Giardia intestinalis are excised by spliceosome-mediated trans-splicing (2011)
  • Author(s): Kamikawa R, Inagaki Y, Tokoro M, Roger AJ, Hashimoto T
  • Journal Title: Cum Biol. Volume: 21 Pages: 1-5
  • Peer Reviewed
• [Journal Article] Comprehensive SSU rRNA phylogeny of Eukaryota (2011)
  • Author(s): Ishida K-I, Inagaki Y, Sakaguchi M, Oiwa A, Kai A, Suzuki M, Nakayama T, Chikuni T, Yabuki A, Yamaguchi H, Yubuki N, Yoshida M, Nakayama T, Inouye I, Hashimoto T
  • Journal Title: J.Endocytobiosis Cell Res. Volume: 20 Pages: 81-88
  • Peer Reviewed
• [Journal Article] Comprehensive SSU rRNA phylogeny of Eukaryota (2010)
  • Author(s): Ishida K, Inagaki Y, Sakaguchi M, Oiwa A, Kai A, Suzuki M, Nakayama T, Chikuni T, Yabuki A, Yamaguchi H, Yubuki N, Yoshida M, Nakayama T, Inouye I, Hashimoto T
  • Journal Title: 【Joint 1st authors】Journal of Endocytobiosis and Cell Research 20 Pages: 81-88
  • Peer Reviewed
• [Journal Article] A wide diversity of previously undetected relatives of diplomonads isolated from marine/saline habitats (2010)
  • Author(s): Kolisko M, Silberman JD, Cepicka I, Yubuki N, Takishita K, Yabuki A, Leander BS, Inouye I, Inagaki Y, Roger AJ, Simpson AGB
  • Journal Title: Environmental Microbiology 12(19) Pages: 2700-2710
  • Peer Reviewed
• [Journal Article] Phylogeny of Asian primate malaria parasites inferred from apicoplast genome-encoded genes with special emphasis on the positions of Plasmodium vivax and P. fraglie (2010)
  • Author(s): Mitsui H, Arisue N, Sakihama N, Inagaki Y, Horii T, Hasegawa M, Tanabe K, Hashimoto T
  • Journal Title: Gene 450(1) Pages: 32-38
  • Peer Reviewed
• [Journal Article] Phylogeny of Asian primate malaria parasites inferred from apicoplast genome-encoded genes with special emphasis on the positions of Plasmodium vivax and P.fragile. (2010)
  • Author(s): Mitsui, H., Arisue, N., Sakihama, N., Inagaki, Y., Horii, T., Hasegawa, M., Tanabe, K., Hashimoto, T.
  • Journal Title: Gene 450 Pages: 32-38
  • Peer Reviewed
• [Journal Article] Gene sampling can bias multi-gene phylogenetic inferences: the relationship between red algae and green plants as the case study (2009)
  • Author(s): Inagaki Y, Nakajima Y, Sato M, Sakaguchi M, Hashimoto T
  • Journal Title: Molecular Biology and Evolution 26(5) Pages: 1171-1178
  • Peer Reviewed
• [Journal Article] Gene sampling can bias multi-gene phylogenetic inferences: the relationships between red algae and green plants as a case study (2009)
  • Author(s): Inagaki, Y., Nakajima, Y., Sato, M., Sakaguchi, M, Hashimoto, T.
  • Journal Title: Molecular Biology and Evolution 26 Pages: 1171-1178
  • Peer Reviewed
• [Journal Article] 複数遺伝子の連結データに基づく分子系統樹の推定-真核生物の大系統の解析を例として (2008)
  • Author(s): 橋本哲男,有末伸子,坂口美亜子,稲垣祐司
  • Journal Title: 統計数理 56 Pages: 145-164
  • Peer Reviewed
• [Journal Article] 複数遺伝子の結合データに基づく分子系統樹の推測-真核生物の大系統の解析を例として- (2008)
  • Author(s): 橋本哲男,有末伸子,坂口美亜子,稲垣祐司
  • Journal Title: 統計数理 56 Pages: 145-164
  • NAID: 120006019006
  • Peer Reviewed
• [Presentation] 寄生性真核微生物Giardiaの縮退ゲノムにおける複雑な遺伝子発現 (2011)
  • Author(s): 神川龍馬、稲垣祐司、Andrew J Roger、橋本哲男
  • Organizer: 第5回日本進化原生生物学研究会
  • Place of Presentation: 富山・富山・富山大学
• [Presentation] 真核生物の系統進化 (2010)
  • Author(s): 橋本哲男
  • Organizer: 33回日本分子生物学会年会・第83回日本生化学会大会合同大会(ワークショップ3W21p)
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-09
• [Presentation] Trans-splicing in the intron-poor eukaryotic parasite Giardia intestinalis (2010)
  • Author(s): Ryoma Kamikawa, Yuji Inagaki, Masaharu Tokoro, Andrew J. Roger, Tetsuo Hashimoto
  • Organizer: 2010 Memorial Symposium for the 26th International Prize for Biology
  • Place of Presentation: 茨城・つくば・国際会議場
• [Presentation] Spliceosome-mediated trans-splicing produces the complete mRNA for heat shock protein 90 in Giardia intestinalis (2010)
  • Author(s): Ryoma Kamikawa, Yuji Inagaki, Masaharu Tokoro, Andrew J. Roger, Tetsuo Hashimoto
  • Organizer: 2010 ISEP XVIII
  • Place of Presentation: 石川・金沢・県立美術館
• [Presentation] 202万樹形に対する網羅的探索結果に基づく発見的樹形探索法の効率評価 (2010)
  • Author(s): 稲垣祐司、辻美和子、Laurent Choy、佐藤三久、橋本哲男
  • Organizer: 第11回日本進化学会
  • Place of Presentation: 北海道・札幌・北海道大
• [Presentation] 複数遺伝子解析による Fornicata 生物群の系統進化の解明 (2010)
  • Author(s): 小松崎洋志、稲垣祐司、瀧下清貴、古我友樹、 矢吹彬憲、松本拓也、神川龍馬、雪吹直史、Martin Kolisko、Alastair Simpson、Andrew Roger、橋本哲男
  • Organizer: 第11回日本進化学会
  • Place of Presentation: 北海道・札幌・北海道大
• [Presentation] 真核生物の系統進化 (2009)
  • Author(s): 橋本哲男
  • Organizer: 日本微生物系統分類研究会第29回年次大会
  • Place of Presentation: 木更津
  • Year and Date: 2009-11-13
• [Presentation] 複数遺伝子解析によるFornicata生物群の系統進化の解明 (2009)
  • Author(s): 小松崎洋志、稲垣祐司、瀧下清貴、古我友樹、矢吹彬憲、松本拓也、神川龍馬、雪吹直史、Martin Kolisko、Alastair Simpson、Andrew Roger、橋本哲男
  • Organizer: 第4回日本進化原生生物研究会
  • Place of Presentation: 宮城・仙台・宮城教育大