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The search for new developmental genes using a gene trap approach in Xenopus tropicalis

Research Project

Project/Area Number 20579002
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Developmental biology
Research InstitutionNara Institute of Science and Technology

Principal Investigator

OGINO Hajime  Nara Institute of Science and Technology, バイオサイエンス研究科, 特任准教授 (10273856)

Co-Investigator(Renkei-kenkyūsha) OCHI Haruki  奈良先端科学技術大学院大学, バイオサイエンス研究科, 特任助教 (00505787)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥3,940,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥540,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥1,600,000 (Direct Cost: ¥1,600,000)
Keywords遺伝学 / 発生・分化 / ゲノム / 遺伝子 / 進化 / 分子生物学 / ツメガエル
Research Abstract

In this study, we attempted a gene trap mutagenesis in Xenopus tropicalis to identify new developmental regulatory genes, using a high-throughput transgenesis technique that we developed recently. The random insertion of a newly designed gene trap vector into the Xenopus genome resulted in generation of an albino mutant, which showed that insertional mutagenesis is actually feasible in Xenopus tropicalis.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (53 results)

All 2010 2009 2008 Other

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (39 results) Remarks (6 results)

  • [Journal Article] Conserved expression of mouse Six1 in the pre-placodal region (PPR) and identification of an enhancer for the rostral PPR.2010

    • Author(s)
      Sato, S., Ikeda, K., Shioi, G., Ochi, H., Ogino, H., Yajima, H., Kawakami, K.
    • Journal Title

      Dev.Biol. 344

      Pages: 158-171

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] The genome of the western clawed frog Xenopus tropicalis.2010

    • Author(s)
      Hellsten, U., Harland, R.M., Gilchrist, M.J., Hendrix, D.Jurka, J., Kapitonov, V., Ovcharenko, I., Putnam, N.H., Shu, S., Taher, L., Blitz, I.L., Blumberg, B., Dichmann, D.S., Dubchak, I., Amaya, E., Fletcher, R., Gerhard, D., Goodstein, D., Graves, T., Grigoriev, I.V., Grimwood, J., Kawashima, T., Lindquist, E., Mead, P.E., Mitros, T., Ogino, H., Ohta, Y., Poliakov, A.V., Pollet, N., Robert, J., Salamov, S., Sater, A.K., Schmutz, J., Terry, S., Vize, P.D., Warren, W.C., Wells, D., Wills, A., Zimmerman, L.B., Zorn, A.M., Grainger, R., Grammer, T., Khokha, M.K., Richardson, P.M.Rokhsar, D.S.
    • Journal Title

      Science 328

      Pages: 633-636

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Evolutionary origin of the Otx2 enhancer for its expression in visceral endoderm.2010

    • Author(s)
      Kurokawa, D., Ohmura, T., Ogino, H., Takeuchi, M., Inoue, A., Inoue, F., Suda, Y.Aizawa, S.
    • Journal Title

      Dev.Biol. 342

      Pages: 110-120

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] The genome of the western clawed frog Xenopus tropicalis2010

    • Author(s)
      Hellsten, Uffe
    • Journal Title

      Science

      Volume: 328 Pages: 633-636

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Conserved expression of mouse Six1 in the pre-placodal region (PPR) and identification of an enhancer for the rostral PPR2010

    • Author(s)
      Sato, Shigeru
    • Journal Title

      Developmental Biology

      Volume: 344 Pages: 158-171

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evolutionary origin of the Otx2 enhancer for its expression in visceral endoderm2010

    • Author(s)
      Kurokawa, Daisuke
    • Journal Title

      Developmental Biology

      Volume: 342 Pages: 110-120

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Resources and transgenesis techniques for functional genomics in Xenopus.2009

    • Author(s)
      Ogino, H.Ochi, Y.
    • Journal Title

      Dev. Growth Differ. 51

      Pages: 387-401

    • NAID

      10025375940

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Resources and transgenesis techniques for functional genomics in Xenopus2009

    • Author(s)
      荻野肇
    • Journal Title

      Development, Growth & Differentiation 51

      Pages: 387-401

    • NAID

      10025375940

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] Evolutionary divergence and conservation of cis-regulatory elements in paralog formation2010

    • Author(s)
      Ochi, H., Uchiyama, C., Kawaguchi, A., Ogino, H.
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-09
    • Related Report
      2010 Final Research Report
  • [Presentation] Conservation and neofunctionalization of cis-regulatory elements in paralog evolution2010

    • Author(s)
      Ogino, H., Ochi, H., Uchiyama, C.
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-09
    • Related Report
      2010 Final Research Report
  • [Presentation] Conservation and neofunctionalization of cis-regulatory elements in paralog evolution2010

    • Author(s)
      荻野肇
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸ポートアイランド(兵庫県神戸市)
    • Year and Date
      2010-12-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] Evolutionary divergence and conservation of cis-regulatory elements in paralog formation2010

    • Author(s)
      越智陽城
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸ポートアイランド(兵庫県神戸市)
    • Year and Date
      2010-12-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] Evolution of a fail-safe regulatory system for kidney development2010

    • Author(s)
      Ochi, H., Uchiyama, C., Kawaguchi, K., Ogino, H.
    • Organizer
      The 16th International Conference of the International Society of Differentiation
    • Place of Presentation
      奈良
    • Year and Date
      2010-11-15
    • Related Report
      2010 Final Research Report
  • [Presentation] Evolution of a fail-safe regulatory system for kidney development2010

    • Author(s)
      越智陽城
    • Organizer
      The 16th International Conference of the International Society of Differentiation
    • Place of Presentation
      奈良県新公会堂(奈良市)
    • Year and Date
      2010-11-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] Evolution of a fail-safe regulatory system after genome duplications in chordates2010

    • Author(s)
      Ochi, H., Uchiyama, C., Kawaguchi, A., Tamai, T., Ogino, H.
    • Organizer
      NAIST Global COE International Symposium 2010 "Plasticity in Development and Evolution"
    • Place of Presentation
      奈良
    • Year and Date
      2010-11-11
    • Related Report
      2010 Final Research Report
  • [Presentation] Evolution of a fail-safe regulatory system after genome duplications in chordates2010

    • Author(s)
      荻野肇
    • Organizer
      NAIST Global COE International Symposium 2010
    • Place of Presentation
      奈良先端科学技術大学院大学(奈良県生駒市)
    • Year and Date
      2010-11-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] 幼生期と成体期のXenopusでみられる四肢再生における異なる開始機構2010

    • Author(s)
      横山仁、丸岡玉枝、越智陽城、有賀章郎, 大湖史、荻野肇、田村宏治
    • Organizer
      平成22年度動物学会東北支部大会
    • Place of Presentation
      福島
    • Year and Date
      2010-08-07
    • Related Report
      2010 Final Research Report
  • [Presentation] 幼生期と成体期のXenopusでみられる四肢再生における異なる開始機構2010

    • Author(s)
      横山仁
    • Organizer
      平成22年度動物学会東北支部大会
    • Place of Presentation
      福島県立医科大学(福島市)
    • Year and Date
      2010-08-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] パラログ形成にともなうシス調節機構の進化2010

    • Author(s)
      荻野肇、越智陽城
    • Organizer
      第12回日本進化学会大会
    • Place of Presentation
      東京
    • Year and Date
      2010-08-05
    • Related Report
      2010 Final Research Report
  • [Presentation] パラログ形成にともなうシス調節機構の進化2010

    • Author(s)
      荻野肇
    • Organizer
      第12回日本進化学会大会
    • Place of Presentation
      東京工業大学(東京都)
    • Year and Date
      2010-08-05
    • Related Report
      2010 Annual Research Report
  • [Presentation] Developmental switch from Rohon-Beard cells to dorsal root ganglia in Xenopus.2010

    • Author(s)
      Yajima, H., Suzuki, M., Ochi, H., Ikeda, K., Sato, S., Ogino, H., Ueno, N.Kawakami, K.
    • Organizer
      第43回日本発生生物学会大会
    • Place of Presentation
      京都
    • Year and Date
      2010-06-21
    • Related Report
      2010 Final Research Report
  • [Presentation] A fail-safe regulatory system generated by genome duplications for kidney development2010

    • Author(s)
      越智陽城荻野肇
    • Organizer
      第43回日本発生生物学会大会
    • Place of Presentation
      京都
    • Year and Date
      2010-06-21
    • Related Report
      2010 Final Research Report
  • [Presentation] Evolution of cis-regulatory mechanisms in paralog formation2010

    • Author(s)
      越智陽城荻野肇
    • Organizer
      第43回日本発生生物学会大会
    • Place of Presentation
      京都
    • Year and Date
      2010-06-21
    • Related Report
      2010 Final Research Report
  • [Presentation] Evolution of cis-regulatory mechanisms in paralog formation2010

    • Author(s)
      荻野肇
    • Organizer
      日本発生生物学会第43回年会
    • Place of Presentation
      京都国際会議場(京都市)
    • Year and Date
      2010-06-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] A fail-safe regulatory system generated by genome duplications for kidney development2010

    • Author(s)
      越智陽城
    • Organizer
      日本発生生物学会第43回年会
    • Place of Presentation
      京都国際会議場(京都市)
    • Year and Date
      2010-06-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] Developmental switch from Rohon-Beard cells to dorsal root ganglia in Xenopus2010

    • Author(s)
      矢嶋浩
    • Organizer
      日本発生生物学会第43回年会
    • Place of Presentation
      京都国際会議場(京都市)
    • Year and Date
      2010-06-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] Different mechanisms in inhibition of limb regeneration between larval and adult Xenopus2010

    • Author(s)
      Yokoyama, H., Ohgo, S., Aruga, A., Muraoka, T., Ochi, H., Ogino, H., Tamura, K.
    • Organizer
      第43回日本発生生物学会大会
    • Place of Presentation
      京都
    • Year and Date
      2010-06-20
    • Related Report
      2010 Final Research Report
  • [Presentation] Different mechanisms in inhibition of limb regeneration between larval and adult Xenopus2010

    • Author(s)
      横山仁
    • Organizer
      日本発生生物学会第43回年会
    • Place of Presentation
      京都国際会議場(京都市)
    • Year and Date
      2010-06-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] Sixl acts as a molecular switch of primary sensory systems from Rohon-beard cells to DRG neurons.2010

    • Author(s)
      Yajima, H., Suzuki, M., 越智陽城, Ikeda, K., Sato, S., Ochi, H., Ueno, N.Kawakami, K.
    • Organizer
      11th Asian and Oceanian Conference on transcription
    • Place of Presentation
      沖縄県今帰仁村
    • Year and Date
      2010-06-04
    • Related Report
      2010 Final Research Report
  • [Presentation] Six1 acts as a molecular switch of primary sensory systems from Rohon-beard cells to DRG neurons2010

    • Author(s)
      川上潔
    • Organizer
      11th Asian and Oceanian Conference on transcription
    • Place of Presentation
      ホテル ベルパライソ(沖縄県今帰仁村)
    • Year and Date
      2010-06-04
    • Related Report
      2010 Annual Research Report
  • [Presentation] Expression and functional analysis of epigenetic regulators, Eed, Ezh2, Jmjd3 and Utx, in Xenopus embryonic development2009

    • Author(s)
      Okano, M., Kawaguchi, A., Ochi, H., Ogino, H.
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2009-12-11
    • Related Report
      2010 Final Research Report
  • [Presentation] Expression and functional analysis of epigenetic regulators, Eed, Ezh2, Jmid3 and Utx, in Xenopus embryonic development2009

    • Author(s)
      岡野誠
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜市
    • Year and Date
      2009-12-11
    • Related Report
      2009 Annual Research Report
  • [Presentation] Xenopusを用いた四肢再生の開始機構に関する研究2009

    • Author(s)
      横山仁、越智陽城、荻野肇、田村宏治
    • Organizer
      第3回日本ツメガエル研究集会
    • Place of Presentation
      広島県廿日市市
    • Year and Date
      2009-09-19
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] Wntシグナリングは四肢再生に対して幼生期と成体期で異なる関与をする2009

    • Author(s)
      横山仁、越智陽城、荻野肇、田村宏治
    • Organizer
      日本動物学会第80回大会
    • Place of Presentation
      静岡
    • Year and Date
      2009-09-19
    • Related Report
      2010 Final Research Report
  • [Presentation] カエルの高効率トランスジェニックシステムを用いた機能ゲノム学的研究の展開2009

    • Author(s)
      荻野肇、越智陽城
    • Organizer
      日本動物学会第80回大会
    • Place of Presentation
      静岡
    • Year and Date
      2009-09-19
    • Related Report
      2010 Final Research Report
  • [Presentation] カエルの高効率トランスジェニックシステムを用いた機能ゲノム学的研究の展開2009

    • Author(s)
      荻野肇
    • Organizer
      日本動物学会第80回大会
    • Place of Presentation
      静岡市
    • Year and Date
      2009-09-19
    • Related Report
      2009 Annual Research Report
  • [Presentation] Wntシグナリングは四肢再生に対して幼生期と成体期で異なる関与をする2009

    • Author(s)
      横山仁
    • Organizer
      日本動物学会第80回大会
    • Place of Presentation
      静岡市
    • Year and Date
      2009-09-19
    • Related Report
      2009 Annual Research Report
  • [Presentation] アフリカツメガエル四肢発生・再生過程におけるレチノイン酸のはたらき2009

    • Author(s)
      大湖史朗、横山仁、越智陽城、荻野肇、田村宏治
    • Organizer
      日本動物学会東北支部大会
    • Place of Presentation
      仙台
    • Year and Date
      2009-08-01
    • Related Report
      2010 Final Research Report
  • [Presentation] アフリカツメガエル四肢発生・再生過程におけるレチノイン酸のはたらき2009

    • Author(s)
      大湖史朗
    • Organizer
      日本動物学会東北支部大会
    • Place of Presentation
      仙台市
    • Year and Date
      2009-08-01
    • Related Report
      2009 Annual Research Report
  • [Presentation] Different involvement of Wnt/b-catenin signaling in limb regeneration of larval and adult Xenopus2009

    • Author(s)
      Yokoyama, H., Ochi, H., Ogino, H., Tamura, K.
    • Organizer
      日本発生生物学会第42回大会
    • Place of Presentation
      新潟
    • Year and Date
      2009-05-29
    • Related Report
      2010 Final Research Report
  • [Presentation] Cis-regulatory mechanisms controlling Six1 expression in the preplacodal region and sensory placodes2009

    • Author(s)
      Sato, S., Ikeda, K., Hayashibara, Y., Nakao, K., Aizawa, S., Ochi, H., Ogino, H., Kawakami, K.
    • Organizer
      日本発生生物学会第42回大会
    • Place of Presentation
      新潟
    • Year and Date
      2009-05-29
    • Related Report
      2010 Final Research Report
  • [Presentation] Cis-regulatory mechanisms controlling Sixl expression in the preplacodal region and sensory placodes2009

    • Author(s)
      佐藤滋
    • Organizer
      日本発生生物学会第42回大会
    • Place of Presentation
      新潟市
    • Year and Date
      2009-05-29
    • Related Report
      2009 Annual Research Report
  • [Presentation] Differential involvement of Wnt/beta-catenin signaling in limb regeneration of larval and adult Xenopus2009

    • Author(s)
      横山仁
    • Organizer
      日本発生生物学会第42回大会
    • Place of Presentation
      新潟市
    • Year and Date
      2009-05-29
    • Related Report
      2009 Annual Research Report
  • [Presentation] Genome-wide analysis of vertebrate cis-regulatory elements in Xenopus2008

    • Author(s)
      荻野肇
    • Organizer
      The 16th CDB Meeting, Cis-sequence regulation and evolution
    • Place of Presentation
      神戸
    • Year and Date
      2008-09-29
    • Related Report
      2010 Final Research Report
  • [Presentation] Genome-wide analysis of vertebrate cis-regulatory elements in Xenopus2008

    • Author(s)
      荻野肇
    • Organizer
      The 16th CDB Meeting Cis-sequence Regulation and its Evolution
    • Place of Presentation
      神戸市
    • Year and Date
      2008-09-29
    • Related Report
      2008 Annual Research Report
  • [Presentation] Xenopus tropicalis を用いた研究の現在の状況と将来の方向性について2008

    • Author(s)
      荻野肇
    • Organizer
      日本発生生物学会第41回大会
    • Place of Presentation
      徳島
    • Year and Date
      2008-05-27
    • Related Report
      2010 Final Research Report
  • [Presentation] Xenopus tropicalisを用いた研究の現在の状況と将来の方向性について2008

    • Author(s)
      荻野肇
    • Organizer
      第41回日本発生生物学会大会
    • Place of Presentation
      徳島市
    • Year and Date
      2008-05-27
    • Related Report
      2008 Annual Research Report
  • [Remarks] ホームページ等

    • URL

      http://bsgcoe.naist.jp/special-grp03.html

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://bsgcoe.naist.jp/special-grp03.html

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://bsgcoe.naist.jp/ogino/index.html

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://bsgcoe.naist.jp/special-grp03.html

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://bsgcoe.naist.jp/ogino/index.html

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://bsgcoe.naist.jp/special-grp03.html

    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-11-18  

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