Characteristics of small GTP binding protein in insect brain

• Project/Area Number: 20580053
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied entomology
• Research Institution: Kobe University
• Principal Investigator: UNO Tomohide Kobe University, 農学研究科, 准教授 (80240852)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 養蚕 / 蚕糸 / 昆虫 / rab / Bombyx mori / 抗体 / 組織染色 / 生物時計 / 微小脳 / カイコ / 脳 / 低分子量GTP結合蛋白質

Research Abstract

Insect synthesizes neuropeptides and clock-related proteins in the specific cell of small brain. These proteins are synthesized upon stimulation of light and temperature. Finally these proteins cause diapause, eclosion and metamorphosis. To elucidate mechanism of synthesis and secretion of these proteins in the brain, we clarified functional roles of Rab proteins ; the small GTPase family to play essential roles in various aspects of membrane traffic ; in the brain of Bombyx mori.

Research Products

• [Journal Article] Small GTPases of the Rab family in the brain of Bombyx mori Histochemistry and Cell Biology (2010)
  • Author(s): Uno, T., Hata, K., Hiragaki, S., Isoyama, Y., Trang, L.T.D., Uno, Y., Kanamaru, K., Yamagata, H., Nakamura, M., Takagi, M., Takeda, M.
  • Journal Title: 134 Pages: 615-622
  • Peer Reviewed
• [Journal Article] Rab14 from Bombyx mori (Lepidoptera : Bombycidae) shows ATPase activity. (2010)
  • Author(s): Uno, T., Moriwaki, T., Isoyama, Y., Uno, Y., Kanamaru, K., Yamagata, H., Nakamura, M., Takagi, M.
  • Journal Title: Biology Letters 6 Pages: 379-381
  • Peer Reviewed
• [Journal Article] Small GTPases of the Rab family in the brain of Bombyx mori (2010)
  • Author(s): 宇野知秀
  • Journal Title: Histochemistry and Cell Biology Volume: 134 Pages: 615-622
  • Peer Reviewed
• [Journal Article] Rab14 from Bombyx mori (Lepidoptera : Bombycidae) shows ATPase activity. (2010)
  • Author(s): 宇野知秀
  • Journal Title: Biology Letters Volume: 6 Pages: 379-381
  • Peer Reviewed
• [Journal Article] Ferulic acid production in the brewing of rice wine (sake). (2009)
  • Author(s): Uno, T., Itoh, O., Miyamoto, T., Kubo, M., Kanamaru, K., Yamagata, H., Yasufuku,Y., Imaishi, H.
  • Journal Title: Journal of the Institute of Brewing. 115 Pages: 116-121
  • Peer Reviewed
• [Journal Article] Phosphorylation of small GTPases, Rab proteins from Bombyx mori (Lepidoptera : Bombycidae). (2009)
  • Author(s): Uno, T., Hata, K., Trang, L.T.D., Hiragaki, S., Nakada, T., Nakamura, M., Yamagata, H., Kanamaru, K., Takeda, M., Matsubara, M.
  • Journal Title: European Journal of Entomology. 106 Pages: 499-506
  • Peer Reviewed
• [Journal Article] Biochemical characterization of Rab proteins from Bombyx mori (2009)
  • Author(s): Uno, T., Moriwaki, T., Nakamura, M., Matsubara, M., Yamagata, H., Kanamaru, K., Takagi, M.
  • Journal Title: Archives of Insect Biochemistry and Physiology 70 Pages: 77-89
  • Peer Reviewed
• [Journal Article] Ferulic Acid Production in the Brewing of Rice Wine(Sake) (2009)
  • Author(s): 宇野知秀
  • Journal Title: JOURNAL OF THE INSTITUTE OF BREWING 115 Pages: 116-121
  • Peer Reviewed
• [Journal Article] Putative regulatory mechanism of prothoracicotropic hormone(PTTH)secretion in the American cockroach, Periplaneta americana as inferred from co-localization of Rab8, PTTH, and protein kinase C in neurosecretory cells (2009)
  • Author(s): 平垣進
  • Journal Title: Cell Tissue Research 335 Pages: 607-615
  • Peer Reviewed
• [Journal Article] Phosphorylation of small GTPase Rab proteins from Bombyx mori(Lepidoptera : Bombycidae) (2009)
  • Author(s): 宇野知秀
  • Journal Title: European journal of entomology 106 Pages: 499-506
  • Peer Reviewed
• [Journal Article] Biochemical characterization of rab proteins from Bombyx mori (2009)
  • Author(s): 宇野 知秀
  • Journal Title: Archives of insect biochemistry and physiology 70 Pages: 77-89
  • Peer Reviewed
• [Presentation] カイコ低分子量GTP結合タンパク質(Rab)の機能解析 (2011)
  • Author(s): 中村緩至
  • Organizer: 日本蚕糸学会第81回大会
  • Place of Presentation: 東京大学農学部
  • Year and Date: 2011-03-21
• [Presentation] カイコ脳を用いたGTP結合タンパク質(Rab)の免疫組織化学的解析 (2010)
  • Author(s): 磯山侑里・畑圭祐・Le Thi Trang・平垣進・山形裕士・金丸研吾・竹田真木夫・宇野知秀
  • Organizer: 日本蚕糸学会第80回大会
  • Place of Presentation: 信州大学
  • Year and Date: 2010-04-04
• [Presentation] カイコ脳を用いたGTP結合タンパク質(Rab)の免疫組織化学的解析 (2010)
  • Author(s): 磯山侑里
  • Organizer: 日本蚕糸学会第80回大会
  • Place of Presentation: 信州大学繊維学部(長野県)
  • Year and Date: 2010-04-04
• [Presentation] カイコ脳に存在する低分子量GTP結合タンパク質(rab)の機能解析 (2009)
  • Author(s): 畑圭祐、Le Thi Dieu Trang、森脇翼、山形裕士、金丸研吾、竹田真木夫、宇野知秀
  • Organizer: 第79回大会
  • Place of Presentation: 東京農工大学
  • Year and Date: 2009-03-22
• [Presentation] カイコ脳に存在する低分子量GTP結合蛋白質(rab)の機能解析 (2009)
  • Author(s): 畑 圭介
  • Organizer: 日本蚕糸学会第79回大会
  • Place of Presentation: 東京農工大学
  • Year and Date: 2009-03-22
• [Presentation] Rab GTPase, small GTP binding proteins, from the brain of Bombyx mori. (2008)
  • Author(s): Hata K., Trang, L.T.D., Moriwaki T., Nakada, T., Hiragaki S., Takagi, M., Kanamaru K., Yamagata H., Takeda M., Uno T.
  • Organizer: Asia-pacific congress of Sericulture and Insect Biotechnology 2008 (APSERI 2008)
  • Place of Presentation: 名古屋大学
• [Remarks] 科学研究費の社会普及事業[ひらめきときめきサイエンス]
• [Remarks] 昆虫の蛋白質を見てみよう-昆虫を用いた生化学実験 平成23年9月24日実施予定
• [Remarks] 昆虫を見てみよう-カイコの解剖と生化学実験;平成22年9月25日実施
• [Remarks] 昆虫の脳の解明-小さな脳にある不思議な力-平成21年7月25日実施