• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Production of useful compounds by plant cell factories established by metabolic engineering

Research Project

Project/Area Number 20580279
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Agricultural environmental engineering
Research InstitutionNagasaki University

Principal Investigator

KITAMURA Yoshie  Nagasaki University, 環境科学部, 教授 (40108337)

Co-Investigator(Kenkyū-buntansha) YAMAGUCHI Kenichi  長崎大学, 水産学部, 准教授 (90363473)
YAMADA Koji  長崎大学, 大学院・医歯薬学総合研究科, 准教授 (00253469)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2009: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2008: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Keywordsエリート植物細胞 / 有用物質生産 / 代謝工学 / ストレス / 組織培養 / 国際情報交換 / インド / 遺伝子導入
Research Abstract

The productions of medicinally and industrially useful organic compounds were accomplished by plant cells in place of petroleum resource. Both cell cultures and hairy roots harboring or induced new metabolic pathways were established via either genetic engineering or artificial stress application. Among them a genetically engineered clone was able to produce hilly amounts of such a compound that seemed to be possible to apply industrial production. In addition, a clone was found to produce a large amount of new compounds under stress condition.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (31 results)

All 2011 2010 2009 2008

All Journal Article (15 results) (of which Peer Reviewed: 15 results) Presentation (16 results)

  • [Journal Article] Increased furanocoumarin production by Glehnia littoralis roots induced via Agrobacterium rhizogenes infection.2011

    • Author(s)
      Terato M, Ishikawa A, Yamada K, Ozeki Y, Kitamura Y
    • Journal Title

      Plant Biotechnology 28 (in press).

    • NAID

      10029399470

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Increased furanocoumarin production by Glehnia littoralis roots induced via Agrobacterium rhizogenes infection2011

    • Author(s)
      Terato M, Ishikawa A, Yamada K, Ozeki Y, Kitamura Y
    • Journal Title

      Plant Biotechnology

      Volume: Vol.28(In press)

    • NAID

      10029399470

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Iron deficiency induces changes in riboflavin secretion and the mitochondrial electron transport chain in hairy roots of Hyoscyamus albus.2010

    • Author(s)
      Higa A, Mori Y, Kitamura Y
    • Journal Title

      Journal of Plant Physiology 167

      Pages: 870-878

    • NAID

      120006984477

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Iron deficiency induces changes in riboflavin secretion and the mitocho-ndrial electron transport chain in hairy roots of Hyoscyamus albus2010

    • Author(s)
      Higa A, Mori Y, Kitamura Y
    • Journal Title

      J Plant Physiology

      Volume: Vol.167 Pages: 870-878

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evaluation of the potential biological toxicities of aqueous extracts from red tide phytoplankton cultures in in vitro and in vivo systems.2010

    • Author(s)
      Kim D, Cha SH, Sato E, Niwano Y, Kohno M, Jiang Z, Yamasaki Y, Matsuyama Y, Yamaguchi K, Oda T
    • Journal Title

      The Journal of Toxicological Sciences

      Volume: Vol.35 Pages: 591-599

    • NAID

      10026567018

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Iron deficiency induces changes in riboflavin secretion and the mitochondrial electron transport chain in hairy roots of Hyoscyamusalbus2010

    • Author(s)
      Higa A, Mori Y, Kitamura Y
    • Journal Title

      Journal of Plant Physiology 167(In press)

      Pages: 1-9

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Constitutive expression of bergaptol O-methyltransferase in Glehnia littoralis cell cultures.2009

    • Author(s)
      Ishikawa A, Kuma T, Sasaki H, Sasaki N, Ozeki Y, Kobayashi N, Kitamura Y
    • Journal Title

      Plant Cell Report 8

      Pages: 257-265

    • NAID

      120006981789

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] HCHL expression in hairy roots of Beta vulgaris yields a high accumulation of p-hydroxybenzoic acid (pHBA) glucose ster, and linkage of pHBA into cell walls.2009

    • Author(s)
      Rahman L, Kouno H, Hashiguchi Y, Yamanoto H, Narbad A, Parr A, Walton N, Ikenaga T, Kitamura Y
    • Journal Title

      Bioresource Technolgy 100

      Pages: 4836-4842

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Constitutive expression of bergaptol O-methyltransferase in Glehnia littoralis cell cultures2009

    • Author(s)
      Ishikawa A, Kuma T, Sasaki H, Sasaki N, Ozeki Y, Kobayashi N, Kitamura Y
    • Journal Title

      Plant Cell Report 28

      Pages: 257-265

    • NAID

      120006981789

    • Related Report
      2009 Annual Research Report 2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] HCHL expression in hairy roots of Beta vulgaris yields a high accumulation of p-hydroxybenzoic acid(pHBA)glucose ester, and linkage of pHBA into cell walls2009

    • Author(s)
      Rahman L, Kono H, Hashiguchi Y, Yamamoto H, Narbad A, Parr J, Walton N, Ikenaga T, Kitamura Y
    • Journal Title

      Bioresource Technology 100

      Pages: 4836-4842

    • NAID

      120006984055

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Root tip-dependent, active riboflavin secretion by Hyoscyamus albus hairy roots under iron deficiency.2008

    • Author(s)
      Higa A, Miyamoto E, Rahman LU, Kitamura Y.
    • Journal Title

      Plant hysiology and Biochemistry 46

      Pages: 452-460

    • NAID

      120006981101

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Enzymatic preparation of 1-O-hydroxycinnamoyl-β-D-glucoses and their application to the study of 1-O-ydroxycinnamoyl-?β-D-glucoase-dependent acyltransferase in anthocyanin-producing cultured cells of Daucus carota and Glehnia littoralis.2008

    • Author(s)
      Matsuba Y, Okuda Y, Abe Y, Kitamura Y, Terasaka K, Mizukami H, Kamakura H, Kawahara N, Goda Y, Sasaki N, Ozeki Y
    • Journal Title

      Plant Biotechnology 25

      Pages: 369-375

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Ascorbic acid induces furanocoumarin production in organ cultures of Glehnia littoralis.2008

    • Author(s)
      Ishikawa A, Kobayashi N, Kitamura Y
    • Journal Title

      Planta Medica 4

      Pages: 1517-1519

    • NAID

      120006982282

    • URL

      http://naosite.lb.nagasaki-u.ac.jp/

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Ascorbic acid induces furanocoumarin production in organ cultures of Glehnia littoralis2008

    • Author(s)
      Ishikawa A, Kobayashi, Kitamura Y
    • Journal Title

      Planta medica 74

      Pages: 1517-1519

    • NAID

      120006982282

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enzymatic preparation of 1-O-hydroxycinnamoyl-β-D- glucoses and their application to the study of 1-O-hydroxycinnamoyl-β-D-glucose-dependent acyltransferases in anthocyanin-producingcul tured cells of Daucus carota and Glehnia littoralis2008

    • Author(s)
      Matuba Y, Okuda Y, Abe Y, Kitamura Y, Terasaka K, Mizukami H, Kamakura H, Kawahara N, Goda Y, Sasaki N, Ozeki Y
    • Journal Title

      Plant Biotechnology 25

      Pages: 369-375

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] 鉄欠乏下のヒヨス培養根からのリボフラビン放出は呼吸と関係する?2010

    • Author(s)
      比嘉中、森裕子、Jevunnahar Khandakar、北村美江
    • Organizer
      第28回日本植物細胞分子生物学会(仙台)大会
    • Place of Presentation
      仙台市(東北大学農学部)
    • Year and Date
      2010-09-03
    • Related Report
      2010 Annual Research Report
  • [Presentation] Metabolic shifts associated with riboflavin excretion in Hyoscyamusalbus roots under iron deficiency2010

    • Author(s)
      Kitamura Y
    • Organizer
      International Symposium on Current Status and Opportunities in Aromatic & Medicinal Plants
    • Place of Presentation
      Lucknow(India)
    • Year and Date
      2010-02-22
    • Related Report
      2009 Annual Research Report
  • [Presentation] 鉄欠乏下のヒヨス培養根からのリボフラビン放出は呼吸と関係する?2010

    • Author(s)
      比嘉中、森裕子、Jebunnahar Khandakar、北村美江
    • Organizer
      第28回日本植物細胞分子生物学会大会 要旨集p.126 (2Da-08)
    • Place of Presentation
      仙台
    • Related Report
      2010 Final Research Report
  • [Presentation] Metabolic shifts associated with riboflavin excretion in Hyoscyamus albus roots under iron deficiency.2010

    • Author(s)
      Kitamura Y, Higa A, Tamari N, Mori Y, Sakata S
    • Organizer
      International symposium on current status and opportunities in aromatic and medicinal plants.
    • Place of Presentation
      Lucknow (India)
    • Related Report
      2010 Final Research Report
  • [Presentation] 鉄欠乏ストレスにおけるヒヨス培養根のリボフラビン生合成遺伝子の発現2009

    • Author(s)
      比嘉中, 北村美江
    • Organizer
      第27回日本植物細胞分子生物学会大会 要旨集p.126 (2Da-08)
    • Place of Presentation
      神奈川
    • Year and Date
      2009-07-31
    • Related Report
      2010 Final Research Report
  • [Presentation] 鉄欠乏ストレス下におけるヒヨス培養根のリボフラビン生合成関連遺伝子の発現2009

    • Author(s)
      比嘉中、北村美江
    • Organizer
      第27回日本植物細胞分子生物学会大会。シンポジウム
    • Place of Presentation
      日本大学生物資源科学部湘南キャンパス(神奈川県)
    • Year and Date
      2009-07-30
    • Related Report
      2009 Annual Research Report
  • [Presentation] 鉄欠乏ストレス下でのヒョス培養根のリボフラビン分泌と呼吸活性2009

    • Author(s)
      森裕子、比嘉中、北村美江
    • Organizer
      第27回日本植物細胞分子生物学会大会。シンポジウム
    • Place of Presentation
      日本大学生物資源科学部湘南キャンパス(神奈川県)
    • Year and Date
      2009-07-30
    • Related Report
      2009 Annual Research Report
  • [Presentation] ハマボウフウ培養根のストレス化合物誘導に及ぼすアスコルビン酸の影響2009

    • Author(s)
      矢賀部由季野、寺戸政紘、山田耕史、北村美江
    • Organizer
      第27回日本植物細胞分子生物学会大会。シンポジウム
    • Place of Presentation
      日本大学生物資源科学部湘南キャンパス(神奈川県)
    • Year and Date
      2009-07-30
    • Related Report
      2009 Annual Research Report
  • [Presentation] ハマボウフウ培養根のストレス化合物誘導に及ぼすアスコルビン酸の影響2009

    • Author(s)
      矢賀部由季野、寺戸政紘、山田耕史、北村美江
    • Organizer
      第27回日本植物細胞分子生物学会大会 要旨集p.126 (2Da-08)
    • Place of Presentation
      神奈川
    • Related Report
      2010 Final Research Report
  • [Presentation] 鉄欠乏ストレス下でのヒヨス培養根のリボフラビン分泌と呼吸活性2009

    • Author(s)
      森裕子、比嘉中, 北村美江
    • Organizer
      第27回日本植物細胞分子生物学会大会 要旨集p.126 (2Da-08)
    • Place of Presentation
      神奈
    • Related Report
      2010 Final Research Report
  • [Presentation] Riboflavin secretion from iron-deficient Hyoscyamus albus root tips2008

    • Author(s)
      Higa A, Miyamoto E, Kitamura Y
    • Organizer
      Plant Metabolism 2008
    • Place of Presentation
      Banff, Alberta, Canada
    • Year and Date
      2008-07-31
    • Related Report
      2008 Annual Research Report
  • [Presentation] HCHL gene expression in hairy roots of Beta vulgaris causes high accumulation of soluble p-Hydroxybenzoic acid derivatives and alters cell wall components2008

    • Author(s)
      Kitamura Y, Rahman L, Hitomi Kawano, Y Hashiguchi NWalton
    • Organizer
      Plant Metabolism 2008
    • Place of Presentation
      Banff, Alberta, Canada
    • Year and Date
      2008-07-31
    • Related Report
      2008 Annual Research Report
  • [Presentation] ニンジン及びハマボウフウ培養細胞におけるアントシアニンアシル基転移酵素の生化学的解析 要旨集p.126 (2Da-08)2008

    • Author(s)
      松崎由紀、奥田裕樹、阿部裕、北村美江、寺坂和洋、水上元、鎌倉浩之、川原信夫、合田幸弘、佐々木伸大、小関良宏
    • Organizer
      第72回日本植物学会
    • Place of Presentation
      高知
    • Related Report
      2010 Final Research Report
  • [Presentation] Riboflavin secretion from iron-deficient Hyoscyamus albus root tips.2008

    • Author(s)
      Higa A, Miyamoto E, Kitamura Y
    • Organizer
      Plant Metabolism 2008
    • Place of Presentation
      Banff, Canada
    • Related Report
      2010 Final Research Report
  • [Presentation] HCHL gene expression in hairy roots of Beta vulgaris causes high accumulation of soluble p-Hydroxybenzoic acid derivatives and alters cell wall components.2008

    • Author(s)
      Kitamura Y, Rahman L, Kawano H, Hashiguchi Y, Walton N
    • Organizer
      Plant Metabolism 2008
    • Place of Presentation
      Banff, Canada
    • Related Report
      2010 Final Research Report
  • [Presentation] ニンジン及びハマボウフウ培養細胞におけるアントシアニンアシル基転移酵素の基質特異性比較2008

    • Author(s)
      松崎由紀、奥田裕樹、阿部裕、北村美江、寺坂和洋、水上元、鎌倉浩之、川原信夫、合田幸弘、佐々木伸大、小関良宏
    • Organizer
      第26回日本植物細胞分子生物学大会 要旨集p.126 (2Da-08)
    • Place of Presentation
      大阪
    • Related Report
      2010 Final Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi