Molecular mechanisms of ABC transporter, Bcrp1, on the regulation of differentiation in mouse embryonic stem cells
Project/Area Number |
20580316
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Applied animal science
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Research Institution | Kinki University |
Principal Investigator |
MITANI Tasuku Kinki University, 先端技術総合研究所, 准教授 (10322265)
|
Co-Investigator(Kenkyū-buntansha) |
SAEKI Kazuhiro 近畿大学, 生物理工学部, 教授 (10298937)
MATSUMOTO Kazuya 近畿大学, 生物理工学部, 教授 (20298938)
TAGUCHI Yoshitomo 近畿大学, 生物理工学部, 講師 (70309269)
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Project Period (FY) |
2008 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | 発生工学 / ES細胞 / ABCトランスポーター / Bcrp1 / 発現制御 / 分化誘導 / Yeast Two Hybrid / リアルタイムPCR |
Research Abstract |
This study examined the function of ABC transporter, Bcrp1, on the differentiation of mouse ES cells. Bcrp1 defines the side population cell phenotype, which indicates the presumptive functional regulator of stem cells. The results may be summarized as follows : (1) the alternative transcription of Bcrp1 mRNA isoforms and analysis of the cis-element, (2) the upregulation of Oct3/4 gene by Bcrp1 overexpression and its induction of ES cells into endodermal cells, and (3) the possibility of Bcrp1 associating with the products of tumor suppressor gene with Bcrp1 protein.
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Report
(4 results)
Research Products
(74 results)
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[Journal Article] In vivo application of an RNAi strategy for the selective suppression of a mutant allele.2011
Author(s)
Kubodera T, Yamada H, Anzai M, Ohira S, Yokota S, Hirai Y, Mochizuki H, Shimada T, 三谷匡, Mizusawa H, Yokota T.
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Journal Title
Human Gene Therapy 22
Pages: 27-34
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Peer Reviewed
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[Journal Article] Mouse androgenetic embryonic stem cells differentiated to multiple cell lineages in three embryonic germ layers in vitro.2009
Author(s)
Teramura T, Onodera Y, Murakami H, Ito S, Mihara T, Takehara T, Kato H, Mitani T, Anzai M, c, Fukuda K, Sagawa N, Hosoi Y.
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Journal Title
J.Reprod Dev. 55
Pages: 283-92
NAID
Related Report
Peer Reviewed
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[Journal Article] Abnormal DNA methylation of the Oct-4 enhancer region in cloned mouse embryos.2009
Author(s)
Kawasumi M, Unno Y, Matsuoka T, Nishiwaki M, Anzai M, Amano T, Mitani T, Kato H, Saeki K, Hosoi Y, Iritani A, Kishigami S, Matumoto
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Journal Title
Mol.Reprod.Dev. 76
Pages: 342-350
Related Report
Peer Reviewed
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[Journal Article] 単為発生胚・雄性発生胚由来胚性幹細胞からの機能的な細胞の分化誘導と解析2008
Author(s)
小野寺勇太,寺村岳士,竹原俊幸,村上秀樹,小澤まどか,武内大輝,安齋政幸,加藤博己,三谷匡,松本和也,佐伯和弘,入谷明,佐川典正,細井美彦
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Journal Title
Mem.Inst.Adv.Technol., Kinki Univ. 13
Pages: 9-19
NAID
Related Report
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[Presentation] マウス初期胚における発生制御タンパク質の解析2010
Author(s)
野老美紀子,川澄みゆり,永井宏平,池上春香,申承旭,西川慧,畑中勇輝,西原卓志,天野朋子,三谷匡,加藤博己,安齋政幸,岸上哲士,佐伯和弘,細井美彦,入谷明,松本和也
Organizer
第103回日本繁殖生物学会大会
Place of Presentation
十和田
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[Presentation] マウス胚性幹細胞の分化過程における細胞核染色体テリトリーと遺伝子座の動態2010
Author(s)
西山有依,森木甲子郎,藤本佑希,安齋政幸,加藤博己,松本和也,佐伯和弘,入谷明,細井美彦,田辺秀之,三谷匡
Organizer
第28回日本受精着床学会総会
Place of Presentation
横浜
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[Presentation] Effects of ABC transporter, Bcrp1, on in vitro differentiation of mouse embryonic stem cells.2009
Author(s)
川村紘子,川合智子,森木甲子郎,田口善智,安齋政幸,加藤博己,細井美彦,入谷明,三谷匡
Organizer
第32回日本分子生物学会年会
Place of Presentation
横浜
Related Report
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[Presentation] プロテオミクスを用いたマウス初期胚における発生関連タンパク質の解析2009
Author(s)
野老美紀子,川澄みゆり,永井宏平,池上春香,申承旭,西川慧,李香欣,畑中勇輝,天野朋子,三谷匡,加藤博己,安齋政幸,岸上哲士,佐伯和弘,細井美彦,入谷明,松本和也
Organizer
第102回日本繁殖生物学会大会
Place of Presentation
奈良
Related Report
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[Presentation] マウス着床前期胚における大規模プロテオーム解析2008
Author(s)
野老美紀子,川澄みゆり,永井宏平,池上春香,佐藤学,申承旭,西川慧,清水なつみ,天野朋子,三谷匡,加藤博己,安齋政幸,岸上哲士,佐伯和弘,細井美彦,入谷明,松本和也
Organizer
第31回日本分子生物学会年会
Place of Presentation
神戸
Related Report
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[Presentation] マウス着床前期胚における大規模プロテオーム解析2008
Author(s)
野老美紀子,川澄みゆり,永井宏平,池上春香,佐藤学,申承旭,西川慧,清水なつみ,天野朋子,三谷匡,加藤博己,安齋政幸,岸上哲士,佐伯和弘,細井美彦,入谷明,松本和也
Organizer
第101回日本繁殖生物学会大会
Place of Presentation
福岡
Related Report
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