| Project/Area Number |
20580316
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | Kinki University |
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Principal Investigator |
MITANI Tasuku Kinki University, 先端技術総合研究所, 准教授 (10322265)
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| Co-Investigator(Kenkyū-buntansha) |
SAEKI Kazuhiro 近畿大学, 生物理工学部, 教授 (10298937)
MATSUMOTO Kazuya 近畿大学, 生物理工学部, 教授 (20298938)
TAGUCHI Yoshitomo 近畿大学, 生物理工学部, 講師 (70309269)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 発生工学 / ES細胞 / ABCトランスポーター / Bcrp1 / 発現制御 / 分化誘導 / Yeast Two Hybrid / リアルタイムPCR |
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| Research Abstract |
This study examined the function of ABC transporter, Bcrp1, on the differentiation of mouse ES cells. Bcrp1 defines the side population cell phenotype, which indicates the presumptive functional regulator of stem cells. The results may be summarized as follows : (1) the alternative transcription of Bcrp1 mRNA isoforms and analysis of the cis-element, (2) the upregulation of Oct3/4 gene by Bcrp1 overexpression and its induction of ES cells into endodermal cells, and (3) the possibility of Bcrp1 associating with the products of tumor suppressor gene with Bcrp1 protein.
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