| Project/Area Number |
20580320
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | Gifu University |
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Principal Investigator |
NAGANO Isao 岐阜大学, 医学系研究科, 准教授 (40283296)
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| Co-Investigator(Kenkyū-buntansha) |
WU Zhiliang 岐阜大学, 医学系研究科, 助教 (90313874)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 旋毛虫 / 筋肉細胞変異 / 筋肉細胞分化 / Rcd1 / AP-1 / NF-κB / myogenin / MyoD / 分子生物学 / Rcdl |
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| Research Abstract |
Trichinella spp. infection causes satellite cell proliferation and transformation of muscle cell to the nurse cell. Rcd1 is shown to be a transcriptional cofactor of the c-myb proto-oncogene product. Previously, we cloned Rcd1 gene from the cDNA of T. spiralis muscle larvae, and confirmed that the Rcd1 protein is part of the E-S products. Therefore, the Rcd1 likely affects host cells and tissues for capsule formation. In the present study we investigated the effect by which the Rcd1protein secreted from T. spiralis modulates myogenic differentiation in C2C12 myoblasts, and the role of the Rcd1 protein regulated NF-κB and AP-1 transcriptional pathway. The expression of myogenin and MyoD genes in C2C12 myoblasts transfected with Rcd1 gene was significantly lower than those in non-transfected C2C12 myoblasts. And we demonstrate that Rcd1 is a potent inhibitor of muscle differentiation through activation(C2C12 cells) of NF-κB and AP-1 signaling pathway. It may be inferred from these results that the Rcd1 protein functions as a transcriptional factor or cofactor of satellite cell proliferation and muscle cell transformation.
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