Cell biological study on dermal pathogenesis of parapoxvirus infection

• Project/Area Number: 20580339
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied veterinary science
• Research Institution: Gifu University
• Principal Investigator: INOSHIMA Yasuo Gifu University, 応用生物科学部, 准教授 (20355184)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: パラポックスウイルス / 血管内皮増殖因子 / ウシ / ヒツジ / ヤギ / ニホンカモシカ / 感染症 / 血管内皮細胞

Research Abstract

Although parapoxvirus (PPV) infections appear in cattle, sheep and goats, the papular, nodular, pustular and ulcerated skin lesions of Japanese serows (Capricornis crispus) are significantly more severe than those of other animals. Our results from cell biological investigations suggest that high responsiveness to viral endothelial growth factor of endothelial cells might be involved in the severe proliferative skin lesions induced by PPV infection in Japanese serows.

Research Products

• [Journal Article] Serological and epidemiological studies of parapoxvirus infection in cattle in Ishikawa Prefecture. (2011)
  • Author(s): Ito, M., Murakami, T., Hayakawa, Y., Shintani, E., Inoshima, Y.
  • Journal Title: Jpn.Agr.Res.Quart. 45 Pages: 123-127
  • NAID: 130002147908
  • URL: http://www.jireas.affre.go.jp/english/publication/jarq/45-1/45-01-17.pdf
  • Peer Reviewed
• [Journal Article] Serological and epidemiological studies of parapoxvirus infection in cattle in Ishikawa Prefecture (2011)
  • Author(s): Ito, M., et al.
  • Journal Title: Japan Agricultural Research Quarterly Volume: 45 Pages: 123-127
  • Peer Reviewed
• [Journal Article] Spatial and temporal genetic homogeneity of orf viruses infecting Japanese serows (Capricornis crispus). (2010)
  • Author(s): Inoshima, Y., Ito, M., Ishiguro, N.
  • Journal Title: J.Vet.Med.Sci. 72 Pages: 701-707
  • NAID: 130000168015
  • URL: http://www.jstage.jst.go.jp/article/jvms/72/6/701/_pdf/-char/ja/
  • Peer Reviewed
• [Journal Article] Molecular and biological characterization of vascular endothelial growth factor of parapoxviruses isolated from wild Japanese serows (Capricornis crispus). (2010)
  • Author(s): Inoshima Y., Tshiguro, N.
  • Journal Title: Vet.Microbiol. 140 Pages: 63-71
  • Peer Reviewed
• [Journal Article] Spatial and temporal genetic homogeneity of orf viruses infecting Japanese serows (Capricornis crispus) (2010)
  • Author(s): Inoshima, Y., et al.
  • Journal Title: Journal of Veterinary Medical Science Volume: 72 Pages: 701-707
  • NAID: 130000168015
  • Peer Reviewed
• [Journal Article] Osteological and genetic analysis of the extinct Ezo wolf (Canis lupus hattah from Hokkaido Island, Japan (2010)
  • Author(s): Ishiguro, N., et al.
  • Journal Title: Zoological Science Volume: 27 Pages: 320-324
  • Peer Reviewed
• [Journal Article] Intron 1 mediated regulation of bovine prion protein gene expression : role of donor splicing sites, sequences with potential enhancer and suppressor activities (2010)
  • Author(s): Elmonir, W., et al.
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 397 Pages: 706-710
  • Peer Reviewed
• [Journal Article] Anti-PrP antibodies detected at terminal stage of prion-affected mouse (2010)
  • Author(s): Sassa, Y., et al.
  • Journal Title: Cellular Immunology Volume: 263 Pages: 212-218
  • Peer Reviewed
• [Journal Article] The effects of lysosomal and proteasomal inhibitors on abnormal forms of prion protein degradation in murine macrophages (2010)
  • Author(s): Sassa, Y., et al.
  • Journal Title: Microbiology and Immunology Volume: 54 Pages: 763-768
  • Peer Reviewed
• [Journal Article] Proteasome activity and biological properties of normal prion protein : acomparison between young and aged cattle (2010)
  • Author(s): Yoshioka, Y., et al.
  • Journal Title: Journal of Veterinary Medical Science Volume: 72 Pages: 1583-1587
  • Peer Reviewed
• [Journal Article] Molecular and biological characterization of vascular endothelial growth factor of parapoxviruses isolated from wild Japanese serows(Capricornis crispus) (2010)
  • Author(s): Inoshima, Y., et al.
  • Journal Title: Veterinary Microbiology 140 Pages: 63-71
  • Peer Reviewed
• [Journal Article] Bovine macrophage degradation of scrapie and BSE PrP^<sc> (2010)
  • Author(s): Sassa, Y., et al.
  • Journal Title: Veterinary Immunology and Immunopathology 333 Pages: 33-39
  • Peer Reviewed
• [Journal Article] Establishment of vascular endothelial cell lines from the aortas of wild Japanese serows (Capricornis crispus). (2009)
  • Author(s): Inoshima, Y., Ishiguro, N.
  • Journal Title: Cell Biol.Int. 33 Pages: 617-620
  • Peer Reviewed
• [Journal Article] Severe dermatitis on cattle teats caused by bovine papular stomatitis virus. (2009)
  • Author(s): Inoshima Y., Nakane, T., Sentsui, H.
  • Journal Title: Vet.Rec. 164 Pages: 311-312
  • URL: http://veterinaryrecord.bmj.com/content/164/10/311.3.full?sid=499ab297-8b2e-4cb0-9441-e8b42479141e
  • Peer Reviewed
• [Journal Article] Molecular characterization of parapoxviruses from infected wild Japanese serows (Capricornis crispus) in Ishikawa Prefecture. (2009)
  • Author(s): Ito, M., Hayakawa, Y., Shintani, E., Inoshima, Y.
  • Journal Title: Jpn.J.Zoo Wildl.Med. 14 Pages: 103-106
  • Peer Reviewed
• [Journal Article] Establishment of vascular endothelial cell lines from the aortas of wild Japanese serows(Capricornis crispus) (2009)
  • Author(s): Inoshima, Y, et al.
  • Journal Title: Cell Biology International 33 Pages: 617-620
  • Peer Reviewed
• [Journal Article] Severe dermatitis on cattle teats caused by bovine papular stomatitis virus (2009)
  • Author(s): Inoshima, Y., et al.
  • Journal Title: Veterinary Record 164 Pages: 311-312
  • Peer Reviewed
• [Journal Article] Isolation and characterization of monoclonal antibldies against bovine serum amyloud A1 protein (2009)
  • Author(s): Taira, Y., et al.
  • Journal Title: Amyloid 16 Pages: 215-220
  • Peer Reviewed
• [Journal Article] Immune cell types involved in early uptake and transport of recombinant mouse prion protein in Peyer's patches of calves (2009)
  • Author(s): Sein Lwin, et al.
  • Journal Title: Cell and Tissue Research 338 Pages: 343-354
  • Peer Reviewed
• [Journal Article] Uptake and transport of foreign particles in Peyer's pactches of both distal ileum and jejunum of calves (2009)
  • Author(s): Sein Lwin, et al.
  • Journal Title: Cell and Tissue Research 337 Pages: 125-135
  • Peer Reviewed
• [Journal Article] Mitochondrial DNA analysis of the Japanese wolf (Canis lupus hodophilax Temminck, 1839) and comparison with representative wolf and domestic dog haplotypes (2009)
  • Author(s): Ishiguro, N., et al.
  • Journal Title: Zoological Science 26 Pages: 765-770
  • Peer Reviewed
• [Journal Article] Molecular characterization of chicken prion proteins by C-termanal-specific monoclonal antibodies (2009)
  • Author(s): Ishiguro, N., et al.
  • Journal Title: Veterinary Immunology and Immunopathology 128 Pages: 402-406
  • Peer Reviewed
• [Journal Article] Molecular characterization of Parapoxviruses from infected Japanese serows(Capricornis crispus) in Ishikawa Prefecture (2009)
  • Author(s): Ito, M., et al.
  • Journal Title: Japanese Journal of Zoo and Wildlife Medicine 14 Pages: 103-106
  • Peer Reviewed
• [Journal Article] Construction of three‐year genetic profile of Japanese wild boars in Wakayama prefecture, to estimate gene flow from crossbred Inobuta into wild hoar populations (2008)
  • Author(s): Ishiguro, N., et al.
  • Journal Title: Mammal Study 33 Pages: 43-49
  • Peer Reviewed
• [Journal Article] Localization of sympathetic, parasympathetic and sensory neurons innervating the distal ileum of the cattle (2008)
  • Author(s): Ohmori, Y., et al.
  • Journal Title: J. Vet. Med. Sci. 70 Pages: 1289-1294
  • Peer Reviewed
• [Presentation] 牛プリオン遺伝子の発現に影響するイントロン1の役割 (2010)
  • Author(s): 石黒直隆, ら
  • Organizer: 第150回日本獣医学会
  • Place of Presentation: 帯広
  • Year and Date: 2010-09-16
• [Presentation] Intron 1 and splicing sites are key regulators for expression of the bovine prion protein gene (2010)
  • Author(s): Elmonir, W., et al.
  • Organizer: Asia-Oceania Symposium on Prion Diseases 2010
  • Place of Presentation: SapPoro, Japan
  • Year and Date: 2010-07-24
• [Presentation] ゴマフアザラシにおけるパラポックスウイルス感染 (2010)
  • Author(s): 猪島康雄、大野佳、前田健、石黒直隆
  • Organizer: 第150回日本獣医学会
  • Place of Presentation: 帯広
• [Presentation] ゴマフアザラシにおけるパラポックスウイルス感染 (2010)
  • Author(s): 大野佳、猪島康雄、前田健、石黒直隆
  • Organizer: 第16回日本野生動物医学会
  • Place of Presentation: 博多
• [Presentation] 石川県の牛におけるパラポックスウイルスの浸潤状況 (2009)
  • Author(s): 伊藤美加、早川裕二、村上俊明、新谷英一、猪島康雄
  • Organizer: 平成21年度中部獣医師会連合会大会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-09-06
• [Presentation] 石川県の牛におけるパラポックスウイルスの浸潤状況 (2009)
  • Author(s): 伊藤美加, ら
  • Organizer: 平成21年度中部獣医師会連合会大会
  • Place of Presentation: 名古屋、愛知
  • Year and Date: 2009-09-06
• [Presentation] 最近流行しているパラポックスウイルスのエンベロープ、VEGF、VIR遺伝子の保存性 (2009)
  • Author(s): 猪島康雄、石黒直隆
  • Organizer: 第148回日本獣医学会
  • Place of Presentation: 鳥取
• [Presentation] PrP^<Sc> degradation pathway in macrophages (2009)
  • Author(s): Sassa, Y., et al.
  • Organizer: Prion 2009
  • Place of Presentation: Porto Carras, Greece
• [Presentation] 最近流行しているパラポックスウイルスのエンベロープ、VEGF VIR遺伝子の保存性 (2009)
  • Author(s): 猪島康雄, ら
  • Organizer: 第148回日本獣医学会
  • Place of Presentation: 鳥取、鳥取
• [Presentation] 家畜ブタを含めたイノシシ属の系統とその起源 (2009)
  • Author(s): 石黒直隆, ら
  • Organizer: 第148回日本獣医学会
  • Place of Presentation: 鳥取、鳥取
• [Presentation] 牛と羊の小腸における外来性神経支配様式の相違 (2009)
  • Author(s): 大森保成, ら
  • Organizer: 第111回日本畜産学会
  • Place of Presentation: 那覇、沖縄
• [Presentation] ニホンカモシカ由来血管内皮細胞株の樹立とバイオアッセイ (2008)
  • Author(s): 猪島康雄、石黒直隆
  • Organizer: 第146回日本獣医学会
  • Place of Presentation: 宮崎
• [Presentation] Role of viral vascular endothelial growth factor in parapoxvirus infection in Japanese serows (Capricornis crispus). (2008)
  • Author(s): 猪島康雄, 石黒直隆
  • Organizer: 3rd International Meeting on Asian ZOO/Wildlife Medicine and Conservation 2008.
  • Place of Presentation: Bogor, Indonesia.
• [Presentation] A case report; contagious pustular dermatitis (parapox infection) in a Japanese serow. (2008)
  • Author(s): Yanai, T., Sakai, H., Inoshima Y., Masegi, T.
  • Organizer: 3rd International Meeting on Asian Zoo/Wildlife Medicine and Conservation 2008.
  • Place of Presentation: Bogor, Indonesia.
• [Presentation] Role of viral vascular endothelial growth factor in parapoxvirus infection in Japanese serows (Capricornis crispus) (2008)
  • Author(s): Inoshima, Y., Ishiguro, N.
  • Organizer: 3^<rd> International Meeting on Asian Zoo/Wildlife Medicine and Conservation 2008
  • Place of Presentation: Bogor, Indonesia
• [Presentation] Mitochondrial DNA variation of Indonesia wild boar (2008)
  • Author(s): Prasetyaningtyas, W. E., et al.
  • Organizer: 3^<rd> International Meeting on Asian Zoo/Wildlife Medicine and Conservation 2008
  • Place of Presentation: Bogor, Indonesia
• [Presentation] A case report : contagious pustular dermatitis (parapox infection) in a Japanese serow (2008)
  • Author(s): Yanai, T., Sakai, H., Inoshima, Y., Masegi, T.
  • Organizer: 3^<rd> International Meeting on Asian Zoo/Wildlife Medicine and Conservation 2008
  • Place of Presentation: Bogor, Indonesia
• [Presentation] プリオン感染マウスおよびrMPrP免疫マウスにおける免疫応答とPrP^<Sc>複製阻害効果 (2008)
  • Author(s): 佐々悠木子, 山本実穂, 猪島康雄, 石黒直隆
  • Organizer: 2008年プリオン研究会
  • Place of Presentation: 新得、北海道
• [Presentation] ニホンカモシカ由来血管内皮細胞株の樹立とバイオアッセイ (2008)
  • Author(s): 猪島康雄, 石黒直隆
  • Organizer: 第146回日本獣医学会
  • Place of Presentation: 宮崎市、宮崎
• [Presentation] 絶滅した日本のオオカミの形態的特徴と遺伝子からみた分類学上の位置 (2008)
  • Author(s): 石黒直隆, 猪島康雄
  • Organizer: 第146回日本獣医学会
  • Place of Presentation: 宮崎市、宮崎
• [Presentation] 牛回腸での物質の取り込みに関与する免疫系細胞の役割 (2008)
  • Author(s): Sein Lwin, 佐々悠木子, 上野博史, 猪島康雄, 石黒直隆
  • Organizer: 第146回日本獣医学会
  • Place of Presentation: 宮崎市、宮崎
• [Presentation] プリオン感染マウスおよびrMPrP免疫マウスが保有する抗PrP^<Sc>複製阻害効果 (2008)
  • Author(s): 佐々悠木子, 山本実穂, 猪島康雄, 石黒直隆
  • Organizer: 第146回日本獣医学会
  • Place of Presentation: 宮崎市、宮崎
• [Presentation] 若齢牛と高齢牛における牛正常型プリオン蛋白質の比較 (2008)
  • Author(s): 吉岡祐美, 猪島康雄, 石黒直隆
  • Organizer: 第146回日本獣医学会
  • Place of Presentation: 宮崎市、宮崎
• [Presentation] 抗体を用いた牛AAアミロイドの性状解析 (2008)
  • Author(s): 平勇人, 猪島康雄, 石黒直隆
  • Organizer: 第146回日本獣医学会
  • Place of Presentation: 宮崎市、宮崎
• [Book] 「ノロウイルスによる食中毒」獣医公衆衛生学実習(獣医公衆衛生学教育研修協議会編) (2010)
  • Author(s): 猪島康雄
  • Publisher: 学窓社
• [Book] ノロウイルスによる食中毒(獣医公衆衛生学実習) (2010)
  • Author(s): 猪島康雄
  • Total Pages: 3
  • Publisher: 学窓社
• [Remarks] ホームページ等
  • URL: http://wwwl.gifu-u.ac.jp/~naishigu/index.html
• [Remarks]
  • URL: http://wwwl.gifu-u.ac.jp/naishigu/index.html