Development of vaccine candidate by using global regulator gene-deficient strains of Streptococcus suis.

• Project/Area Number: 20580349
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied veterinary science
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: OSAKI Makoto National Agriculture and Food Research Organization, 動物衛生研究所・細菌・寄生虫病研究チーム, 主任研究員 (80355164)
• Co-Investigator(Renkei-kenkyūsha): SEKIZAKI Tsutomu 東京大学, 農学部, 教授 (70355163) ; TAKAMATSU Daisuke 独立行政法人農業・食品産業技術総合研究機構, 動物衛生研究所・細菌・寄生虫病研究チーム, 主任研究員 (60414728)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 疾病予防・制御 / 獣医細菌学 / 分子遺伝学 / ワクチン開発 / 疾病予防制御

Research Abstract

Streptococcus suis is an important pathogen in pig industry and also a causative agent of zoonosis. In this study, we generated deletion mutants of several global regulator genes from virulent strains of S. suis. One of those strains, a caseinolytic protease gene (clpP)-deletion mutant derived from S. suis P1/7 strain, was shown to be attenuated in pigs and possess ability for colonization at tonsil of pigs. Our results suggest that the S. suis clpP-deletion mutant strain can be a live-vaccine candidate for S. suis infection in pigs.

Research Products

• [Presentation] 豚レンサ球菌ClpPプロテアーゼ遺伝子欠損株の性状解析 (2008)
  • Author(s): 大崎慎人, 大倉正稔, 高松大輔, 下地善弘, 関崎勉
  • Organizer: 第91回日本細菌学会関東支部総会
  • Place of Presentation: 生命の森リゾート日本エアロビクスセンター
  • Year and Date: 2008-10-23
• [Presentation] (2008)
  • Organizer: 第91回日本細菌学会関東支部会抄録(p49)
  • Place of Presentation: (口頭発表)