Regulatory mechanisms for the proliferation and invasion under oxidative stress via modulation of membranous glycolipids.

• Project/Area Number: 20580369
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied molecular and cellular biology
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: MIURA Yutaka Tokyo University of Agriculture and Technology, 大学院・農学研究院, 准教授 (10219595)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 癌細胞浸潤 / 酸化ストレス / 細胞膜糖脂質 / ガングリオシド / 癌細胞増殖 / 糖脂質 / 細胞膜 / 癌転移

Research Abstract

The regulatory mechanisms for the protentiation of mouse melanoma cell invasion under hypoxic condition were investigated. The increase in membranous ganglioside GM3 contents and the modulation of proteins-glycolipids complex in cell membrane were proved to be deeply involved in this phenomenon. This result indicates novel mechanisms for cancer aggravation under hypoxia.

Research Products

• [Journal Article] Hypoglycemic effect of resveratrol in type 2 diabetic model db/db mice and its actions in cultured L6 myotubes and RIN-5F pancreatic b-cells. (2011)
  • Author(s): Minakawa M, Kawano A, Miura Y, Yagasaki K
  • Journal Title: J.Clin.Biochem.Nutr. 48 Pages: 237-244
  • Peer Reviewed
• [Journal Article] Anti-Invasive Activity of 1-Tocopherol against Hepatoma Cells in Culture via Protein Kinase C Inhibition. (2011)
  • Author(s): Yoshida S, Hirakawa N, Ito K, Miura Y, Yagasaki K
  • Journal Title: J.Clin.Biochem.Nutr. 48 Pages: 251-257
  • Peer Reviewed
• [Journal Article] Effects of sulfur amino acids, L-cysteine on lipoprotein lipase and hormone- sensitive lipase in differentiated mouse 3T3-L1 adipocytes. (2010)
  • Author(s): Kawasaki M, Miura Y, Yagasaki K
  • Journal Title: Cytotechnology 62 Pages: 225-233
  • Peer Reviewed
• [Journal Article] Yagasaki K Effects of simultaneous dietary fish oil ingestion and sulfur amino acid supplementation on the lipid metabolism in hepatoma-bearing rats with hyperlipidemia. (2010)
  • Author(s): Kawasaki M, Miura Y, Funabiki R
  • Journal Title: J Nutr.Sci.Vitaminol. 56 Pages: 247-254
  • Peer Reviewed
• [Journal Article] Effects of sulfur amino acids, L-cysteine on lipoprotein lipase and horm one-sensitive lipase in differentiated mouse 3T3-L1 adipocytes. (2010)
  • Author(s): Masashi Kawasaki
  • Journal Title: Cytotechnology Volume: 62 Pages: 225-233
  • Peer Reviewed
• [Journal Article] Hypoglycemic effect of resveratrol in type 2 diabetic model db/db mice and its actions in cultured L6 myotubes and RIN-5F pancreatic β-cells. (2010)
  • Author(s): Miki Minakawa
  • Journal Title: J.Clin.Biochem.Nutr. Volume: (in press)
  • Peer Reviewed
• [Journal Article] Anti-invasive activity of α-tocopherol against hepatoma cells in culture via protein kinase C inhibition. (2010)
  • Author(s): Shunsuke Yoshida
  • Journal Title: J.Clin.Biochem.Nutr. Volume: (in press)
  • Peer Reviewed
• [Journal Article] Parvulin (Par14), a peptidyl-prolyl cis-trans isomerase, is a novel rRNA processing factor that evolved in the metazoan lineage. (2009)
  • Author(s): Fujiyama-Nakamura S, Yoshikawa H, Homma K, Hayano T, Tsujimura, Takahashi T, Izumikawa K, Ishikawa H, Miyazawa N, Yanagida M, Miura Y, Shinkawa T, Yamauchi Y, Isobe T, Takahashi N.
  • Journal Title: Molecular and Cellular Proteomics 8 Pages: 1552-1565
  • Peer Reviewed
• [Journal Article] Hypoglycemic effect of aspalathin, a rooibos tea component from Aspalathus linearis, in type 2 diabetic model db/db mice. (2009)
  • Author(s): Kawano A, Nakamura H, Hata S, Minakawa M, Miura Y, Yagasaki K.
  • Journal Title: Phytomedicine 16(5) Pages: 437-443
  • Peer Reviewed
• [Journal Article] 糖脂質、とくにガングリオシドによる抗がん・免疫調節作用について (2009)
  • Author(s): 三浦豊
  • Journal Title: オレオサイエンス 第9巻、第10号 Pages: 473-481
  • NAID: 130004853515
• [Journal Article] Hypoglycemic effect of aspalathin, a rooibos tea component from Aspalathus linearis, in type 2 diabetic model db/db mice (2009)
  • Author(s): Kawano A.
  • Journal Title: Phytomedicine 16 Pages: 437-443
  • Peer Reviewed
• [Journal Article] Parvulin (Par14), a peptidyl prolyl cis-trans isomerase, is a novel rRNA-processing factor evolved in the metazoan lineage (2009)
  • Author(s): Fujiyama-Nakamura S.
  • Journal Title: Mol.Cell.Proteomics 8 Pages: 1552-1565
  • Peer Reviewed
• [Journal Article] Effect of dietary soy protein on tumor necrosis factor productivity in macrophages from nephritic and hepatomabearing rats. (2008)
  • Author(s): Komatsu W, Nagata J, Kaneko M, Yamada T, Moriya D, Miura Y, Yagasaki K.
  • Journal Title: J.Nutr.Sci.Vitaminol. 54 Pages: 435-439
  • NAID: 110007007840
  • Peer Reviewed
• [Journal Article] Effect of dietary soy protein on tumor necrosis factor productivity in macrophages from nephritic and hepatoma-bearing rats. (2008)
  • Author(s): Komatsu w.
  • Journal Title: J Nutr Sci Vitaminol. 54 Pages: 435-439
  • Peer Reviewed
• [Journal Article] Inhibitory effect of gingerol on the proliferation and invasion of hepatoma cells in culture. (2008)
  • Author(s): Yagihashi S.
  • Journal Title: Cytotechnology 57 Pages: 129-136
  • Peer Reviewed
• [Presentation] O-linked N-acetylglucosamine modification plays the important roles in tumor phenotypes under hypoxia (2010)
  • Author(s): Miura Y.
  • Organizer: International Congress of Nutrition 19th
  • Place of Presentation: バンコク、タイ
  • Year and Date: 2010-10-06
• [Presentation] Regulation of mouse melanoma invasion by membranous ganglioside GM3. (2010)
  • Author(s): Yosuke Oikawa, Manami Miyake, Kazumi Yagasaki, Yagasaki Miura
  • Organizer: JAACT2010
  • Place of Presentation: Sapporo, Japan
  • Year and Date: 2010-09-03
• [Presentation] Regulation of mouse melanoma invasion by membranous ganglioside GM3 (2010)
  • Author(s): Yosuke Oikawa
  • Organizer: JAACT 2010
  • Place of Presentation: 北海道大学、札幌
  • Year and Date: 2010-09-03
• [Presentation] 低酸素培養条件下における癌細胞浸潤能亢進の機構解析 (2010)
  • Author(s): 三村真吾、矢ヶ崎一三、三浦豊
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-29
• [Presentation] O-linked N-acetylglucosamine modification plays the important roles in tumor phenotypes under hypoxia. (2009)
  • Author(s): Yutaka Miura, Boonchoo Wannachanok, Shingo Mimura, Kazumi Yagasaki
  • Organizer: The 19th International Congress of Nutrition
  • Place of Presentation: Bangkok, Thailand
  • Year and Date: 2009-10-06
• [Presentation] 低酸素状態下での癌細胞浸潤能亢進への細胞内タンパク質O-GlcNAc 修飾の関与 (2009)
  • Author(s): 三村真吾、Wannachanok Boonchoo、矢ヶ崎一三、三浦豊
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡
  • Year and Date: 2009-03-28
• [Presentation] Inhibition of mouse melanoma motility by exogenous ganglioside GM3 and its mode of action (2008)
  • Author(s): Yutaka Miura, 他2名
  • Organizer: The 21^<st> Annual and International Meeting of the Japanese Association for Animal Cell Technology
  • Place of Presentation: 福岡市
  • Year and Date: 2008-11-26