| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Research Abstract |
We found that Bacillus cereus SMase has at least two bind sites for substrate, one at the active site and the other at the allosteric site (surface recognition site). The cooperative binding of substrate to the allosteric site induced the structure change for the activation of SMase. Pro 26, Trp 28, and Asp 100 of B. cereus SMase were found to be participate the surface recognition. Furthermore, we found that a thiophosphate analog of SM, in which the phosphate oxygen was replaced by sulfur atom, can be used for the substrate of the measurement of SMase activity.
|
