The regulatory mechanisms of retinal photoreceptor development

• Project/Area Number: 20590079
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Osaka Bioscience Institute
• Principal Investigator: KOIKE Chieko Osaka Bioscience Institute, 薬学部, 准教授 (80342723)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 発生分化 / 神経科学 / 視細胞 / Crx / 転写制御 / 網膜 / プロモーター / サイレンサー / 松果体 / ホメオボックス

Research Abstract

We performed transgenic mice based promoter analysis to identify which sequence determines the restricted Crx expression. We identified 14bp sequence, named as CUE sequence, in the proximal region of Crx promoter that is required for the specific expression of Crx in photoreceptors. The lack of CUE sequence in Crx promoter resulted in disappearance of lacZ expression in the photoreceptor and pineal grand.

Research Products

• [Journal Article] TRPM1 mutations are associated with the complete form of congenital stationary night blindness. (2010)
  • Author(s): Makoto Nakamura, Rikako Sanuki, Tatsuhiro R.Yasuma, Akishi Onishi, Koji M.Nishiguchi, Chieko Koike, Mikiko Kadowaki, Mineo Kondo, Yozo Miyake, Takahisa Furukawa
  • Journal Title: Mol.Vis. 16 Pages: 425-37
  • Peer Reviewed
• [Journal Article] TRPM1 : A vertebrate TRP channel responsible for retinal ON bipolar function. (2010)
  • Author(s): Chieko Koike, Tomohiro Numata, Hiroshi Ueda, Yasuo Mori, Takahisa Furukawa
  • Journal Title: Cell Calcium(Review). 48(2-3) Pages: 95-101
  • Peer Reviewed
• [Journal Article] Panky, a novel photoreceptor-specific ankyrin repeat protein, is a transcriptional cofactor that suppresses CRX-regulated photoreceptor genes. (2010)
  • Author(s): Sanuki R, Omori Y, Koike C, Sato S, Furukawa T
  • Journal Title: FEBS Lett. 19;584(4) Pages: 753-8
  • Peer Reviewed
• [Journal Article] TRPM1 is a component of the retinal ON bipolar cell transduction channel in the mGluR6 cascade. (2010)
  • Author(s): Chieko Koike, Takehisa Obara, Yoshitsugu Uriu, Tomohiro Numata, Rikako Sanuki, Kentaro Miyata, Toshiyuki Koyasu, Shinji Ueno, Kazuo Funabiki, Akiko Tani, Hiroshi Ueda, Mineo Kondo, Yasuo Mori, Masao Tachibana, Takahisa Furukawa
  • Journal Title: Proc.Natl.Acad.Sci.USA 107(1) Pages: 333-337
  • Peer Reviewed
• [Journal Article] TRPM1 mutations are associated with the complete form of congenital stationary night blindness. (2010)
  • Author(s): Nakamura M, Sanuki R, Yasuma TR, Onishi A, Nishiguchi KM, Koike C, Kadowaki M, Kondo M, Miyake Y, Furukawa T.
  • Journal Title: Mol Vis. Volume: 16 Pages: 425-437
  • Peer Reviewed
• [Journal Article] TRPM1 : A vertebrate TRP channel responsible for retinal ON bipolar function. (2010)
  • Author(s): Koike C, Numata T, Ueda H, Mori Y, Furukawa T.
  • Journal Title: Cell Calcium.(Review) Volume: 48 Pages: 95-101
• [Journal Article] TRPM1 is a component of the retinal ON bipolar cell transducti on channel in the mGluR6 cascade. (2010)
  • Author(s): 小池千恵子、瓜生幸嗣, 他
  • Journal Title: Proc.Natl.Acad.Sci.USA 107 Pages: 333-337
  • Peer Reviewed
• [Journal Article] Panky, a novel photoreceptor-specific ankyrin repeat protein, is a transcriptional cofactor that suppresses CRX-regulated photore ceptor genes. (2010)
  • Author(s): 佐貫理佳子、大森義裕、小池千恵子, 他
  • Journal Title: FEBS Letter 584 Pages: 753-758
  • Peer Reviewed
• [Journal Article] 網膜視細胞の細胞極性と細胞内オルガネラの制御-外節形成、繊毛内輸送、核のポジショニングから網膜変性症まで- (2008)
  • Author(s): 小池千恵子,古川貴久
  • Journal Title: 生化学 80 Pages: 224-232
  • NAID: 10021191259
• [Journal Article] 網膜視細胞の細胞極性と細胞内オルガネラの制御-外節形成、繊毛内輸送、核のポジショニングから網膜変性症まで- (2008)
  • Author(s): 小池千恵子古川貴久
  • Journal Title: 生化学 80 Pages: 224-232
  • NAID: 10021191259
• [Presentation] 網膜ON型双極細胞の視覚伝達チャネルTRPM1の発現メカニズム解析 (2011)
  • Author(s): 遠藤拓真、上田祐輔、多賀駿、古川貴久、小池千恵子
  • Organizer: 日本薬学会第131年会(地震のため中止・学会は成立)
  • Place of Presentation: 静岡県立大学(静岡県)
  • Year and Date: 2011-03-30
• [Presentation] マウス網膜発生過程におけるIRSp53の発現機能解析 (2011)
  • Author(s): 山岡昭士、末次志郎、小池千恵子
  • Organizer: 日本薬学会第131年会(地震のため中止・学会は成立)
  • Place of Presentation: 静岡県立大学(静岡県)
  • Year and Date: 2011-03-29
• [Presentation] 三次元マイクロプローブ電極アレイを用いたマウス網膜光応答解析 (2011)
  • Author(s): 多賀駿、殿村渉、寺前彰二、小西聡、小池千恵子
  • Organizer: 日本薬学会第131年会(地震のため中止・学会は成立)
  • Place of Presentation: 静岡県立大学(静岡県)
  • Year and Date: 2011-03-29
• [Presentation] Electroretinogram recordings inside isolated mouse retina using spatially arranged microelectrodes (2011)
  • Author(s): W.Tonomura, S.Taga, C.Koike, S.Konishi
  • Organizer: 国際ワークショップ
  • Place of Presentation: 東京大学武田ホール(東京都)
  • Year and Date: 2011-03-04
• [Presentation] TRPM1 is a component of the retinal ON bipolar cell transduction channel (2010)
  • Author(s): Chieko Koike, Tomohiro Numata, Hiroshi Ueda, Mineo Kondo, Masao Tachibana, Takahisa Furukawa
  • Organizer: The American Society of Cell Biology
  • Place of Presentation: Philadelphia(U.S.A)
  • Year and Date: 2010-12-13
• [Presentation] TRPM1 is a component of the retinal ON bipolar cell transduction channel in the mGluR6 cascade. (2010)
  • Author(s): 小池千恵子, 他
  • Organizer: 第50回全米細胞生物学会年会
  • Place of Presentation: Philadelphia, U.S.
  • Year and Date: 2010-12-13
• [Presentation] Rod bipolar cells in mGluR6-null mice are hyperpolarized (2010)
  • Author(s): Ying Xu, Marie Fina, 小池千恵子, Takahisa Furukawa, Noga Vardi
  • Organizer: Society for Neuroscience
  • Place of Presentation: San Diego(U.S.A)
  • Year and Date: 2010-11-17
• [Presentation] The function of mouse and human TRPM1 (2010)
  • Author(s): Chieko Koike, Tomohiro Numata, Hiroshi Ueda, Mineo Kondo, Masao Tachibana, Takahisa Furukawa
  • Organizer: FASEB meeting
  • Place of Presentation: Vermont(U.S.A)
  • Year and Date: 2010-07-21
• [Presentation] Functional roles of mouse and human TRPM1 (2010)
  • Author(s): 小池千恵子, 他
  • Organizer: FASEB meeting
  • Place of Presentation: Vermont, U.S.
  • Year and Date: 2010-07-12
• [Presentation] 網膜ON型双極細胞の視覚伝達チャネルTRPM1 (2010)
  • Author(s): 小池千恵子
  • Organizer: 第157回大阪大学 生物科学セミナー
  • Place of Presentation: 大阪大学(大阪府)
  • Year and Date: 2010-06-09
• [Presentation] 網膜ON型双極細胞の視覚伝達チャネルTRPM1 (2010)
  • Author(s): 小池千恵子
  • Organizer: 第157回大阪大学生物科学セミナー
  • Place of Presentation: 大阪大学、大阪
  • Year and Date: 2010-06-09
• [Presentation] 中枢神経系特異的に高発現する網膜非コードRNA3(RNCR3)上に存在するmiR-124a-1ノックアウトマウスの解析 (2009)
  • Author(s): 佐貫理佳子、小池千恵子, 他
  • Organizer: 日本生化学会
  • Place of Presentation: 神戸市 神戸ポートアイランド
  • Year and Date: 2009-10-24
• [Presentation] TRPM1 is a component of the retinal ON bipolar cell transduction channel (2009)
  • Author(s): Chieko Koike, Tomohiro Numata, Hiroshi Ueda, Mineo Kondo, Masao Tachibana, Takahisa Furukawa
  • Organizer: Society for Neuroscience
  • Place of Presentation: Chicago(U.S.A)
  • Year and Date: 2009-10-21
• [Presentation] TRPM1 is a candidate transduction channel for visual signal transmission in retinal ON bipolar cells (2009)
  • Author(s): 小池千恵子, 他
  • Organizer: 全米神経科学会
  • Place of Presentation: シカゴ、米国
  • Year and Date: 2009-10-21
• [Presentation] 網膜ON型双極細胞におけるTRPM1の機能 (2008)
  • Author(s): 小池千恵子, 小原武久, 瓜生幸嗣, 宮田健太郎, 小安俊行, 近藤峰生, 森泰生, 立花政夫, 古川貴久
  • Organizer: 日本神経科学会
  • Place of Presentation: 東京国際フォーラム
  • Year and Date: 2008-07-10
• [Remarks] ホームページ等