Analysis for individual difference in xenobiotic metabolism

• Project/Area Number: 20590121
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental pharmacy
• Research Institution: Okayama University
• Principal Investigator: HANIOKA Nobumitsu Okayama University, 大学院・医歯薬学総合研究科, 准教授 (70228518)
• Co-Investigator(Kenkyū-buntansha): NARIMATSU Shizuo 岡山大学, 大学院・医歯薬学総合研究科, 准教授 (20113037)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 環境化学物質 / 異物代謝酵素 / シトクロムP450(CYP) / UDP-グルクロン酸転移酵素(UGT) / 遺伝子多型 / 個別化危険度評価 / 個別危険度評価 / シトクロムP450 / CYP2E1 / UDP-グルクロン酸転移酵素 / 核内受容体 / 環境的要因 / 遺伝的要因

Research Abstract

The purpose of this study was to clarify whether the genetic polymorphism of CYP2E1 affect the metabolism of environmental chemicals. The findings obtained may mean that the polymorphic alleles of CYP2E1 causing amino acid substitutions are not directly associated with the metabolic activation of benzene and toluene.

Research Products

• [Journal Article] Functional characterization of human cytochrome P450 2E1 allelic variants : invitro metabolism of benzene and toluene by recombinant enzymes expressed in yeast cells. (2010)
  • Author(s): Hanioka N, Yamamoto M, Tanaka-Kagawa T, Jinno H, Narimatsu S.
  • Journal Title: Arch Toxicol 84 Pages: 363-371
• [Journal Article] Functional characterization of human cytochrome P450 2E1 allelic variants : in vitro metabolism of benzene and toluene by recombinant enzymes expressed in yeast cells (2010)
  • Author(s): Hanioka N, Yamamoto M, Tanaka-Kagawa T, Jinno H, Narimatsu S
  • Journal Title: Arch Toxicol Volume: 84 Pages: 363-371
  • Peer Reviewed
• [Journal Article] Functional characterization of human and cynomolgus monkey UDP-glucuronosyltransferase 1A1 enzymes (2010)
  • Author(s): Hanioka N, Tanabe N, Jinno H, Tanaka-Kagawa T, Nagaoka K, Naito S, Koeda A, Narimatsu S
  • Journal Title: Life Sci Volume: 87 Pages: 261-268
  • Peer Reviewed
• [Journal Article] Determination of polycyclic aromatic hydrocarbons in food samples by automated on-line in-tube solid-phase microextraction coupled with high-performance liquid chromatography-fluorescence detection (2010)
  • Author(s): Ishizaki A, Saito K, Hanioka N, Narimatsu S, Kataoka H
  • Journal Title: J Chromatogr A Volume: 217 Pages: 5555-5563
  • Peer Reviewed
• [Journal Article] Determination of aflatoxins in food samples by automated on-line in-tube solid-phase microextraction coupled with liquid chromatography-mass spectrometry. (2009)
  • Author(s): Nonaka Y, Saito K, Hanioka N, Narimatsu S, Kataoka H.
  • Journal Title: J Chromatogr A 1216 Pages: 4416-4422
• [Journal Article] Determination of aflatoxins in food samples by automated on-line in-tube solid-phase microextraction coupled with liquid chromatography-mass spectrometry (2009)
  • Author(s): Nonaka Y, Saito K, Hanioka N, Narimatsu S, Kataoka H.
  • Journal Title: J Chromatogr A 1216 Pages: 4416-4422
  • Peer Reviewed
• [Journal Article] Separate evaluation of intestinal and hepatic metabolism of three benzodiazepines in rats with cannulated portal and jugular veins : comparison with the profile in non-cannulated mice (2009)
  • Author(s): Kuze J, Mutoh T, Takenaka T, Morisaki K, Nakura H, Hanioka N,
  • Journal Title: Xenobiotica 39 Pages: 871-880
  • Peer Reviewed
• [Journal Article] Interaction of bisphenol A with human UDP-glucuronosyltransferase 1A6 enzyme. (2008)
  • Author(s): Hanioka N, Takeda Y, Tanaka-Kagawa T, Hayashi K, Jinno H, Narimatsu S.
  • Journal Title: Environ Toxicol 23 Pages: 407-412
• [Journal Article] Interaction ofbisphenol a with human UDP-glucuronosyltransferase 1A6 enzyme (2008)
  • Author(s): Hanioka N, Takeda Y, Tanaka-Kagawa T, Hayashi K, Jinno H, Narimatsu s.
  • Journal Title: Environ Toxicol 23 Pages: 407-412
  • Peer Reviewed
• [Journal Article] Human UDP-glucuronosyltransferase isoforms involved in bisphenol A glucuronidation (2008)
  • Author(s): Hanioka N, Naito T, Narimatsu s.
  • Journal Title: Chemosphere 74 Pages: 33-36
  • Peer Reviewed
• [Presentation] リン酸エステル系可塑剤・難燃剤のTRPイオンチャネルに対する影響 (2010)
  • Author(s): 香川(田中)聡子, 大河原晋, 古川容子, 埴岡伸光, 西村哲治, 成松鎭雄, 神野透人
  • Organizer: ォーラム2010:衛生薬学・環トキシコロジー
  • Place of Presentation: 東京
  • Year and Date: 2010-09-10
• [Presentation] フタル酸ジエステル類のヒト肝ミクロゾームにおける加水分解反応 (2010)
  • Author(s): 高原佑輔, 埴岡伸光, 香川(田中)聡子, 神野透人, 成松鎭雄
  • Organizer: フォーラム2010:衛生薬学・環境トキシコロジー
  • Place of Presentation: 東京
  • Year and Date: 2010-09-09
• [Presentation] フタル酸ジエステル類のヒト肝ミクロゾームにおける加水分解反応 (2010)
  • Author(s): 高原佑輔, 埴岡伸光, 香川(田中)聡子, 神野透人, 成松鎭雄
  • Organizer: ォーラム2010:衛生薬学・環境トキシコロジー
  • Place of Presentation: 東京
  • Year and Date: 2010-09-09
• [Presentation] フタル酸ジエステル類の代謝に関与するカルボキシルエステラーゼ分子種 (2010)
  • Author(s): 高原佑輔, 埴岡伸光, 香川(田中)聡子, 神野透人, 成松鎭雄
  • Organizer: 第51回日本生化学会中国・四国支部例会
  • Place of Presentation: 山口
  • Year and Date: 2010-05-15
• [Presentation] 有機フッ素化合物PFOSの毒性発現と代謝の関連性 (2009)
  • Author(s): 中西涼子, 埴岡伸光, 齋藤啓太, 片岡洋行, 成松鎭雄
  • Organizer: 日本薬物動態学会第24回年会
  • Place of Presentation: 東京
  • Year and Date: 2009-11-28
• [Presentation] アフラトキシンB1によるHepG2細胞における薬物代謝酵素の変動 (2009)
  • Author(s): 野中結子, 埴岡伸光, 齋藤啓太, 片岡洋行, 成松鎭雄
  • Organizer: 第48回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会
  • Place of Presentation: 徳島
  • Year and Date: 2009-11-08
• [Presentation] Bisphenol Aグルクロン酸抱合反応に及ぼすUGT2B15遺伝子多型の影響 (2009)
  • Author(s): 岡宏之, 埴岡伸光, 森川純, 生城真一, 成松鎭雄
  • Organizer: フォーラム2009 : 衛生薬学・環境トキシコロジー
  • Place of Presentation: 宜野湾
  • Year and Date: 2009-11-05
• [Presentation] 有機フッ素化合物PFOS及びPFOAとヒトシトクロムP450との相互作用 (2008)
  • Author(s): 中西涼子, 埴岡伸光, 齋藤啓太, 片岡洋行, 成松鎮雄
  • Organizer: 第47回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会
  • Place of Presentation: 岡山
  • Year and Date: 2008-11-09
• [Presentation] ベンゼン及びトルエン代謝に及ぼすヒトCYP2E1の遺伝子多型の影響 (2008)
  • Author(s): 埴岡伸光, 山本真紀, 成松鎭雄
  • Organizer: フォーラム2008:衛生薬学・環境トキシコロジー
  • Place of Presentation: 熊本
  • Year and Date: 2008-10-18
• [Presentation] ベンゼン及びトルエン代謝に及ぼすヒトCYP2E1の遺伝子多型の影響 (2008)
  • Author(s): 埴岡伸光, 山本真紀, 成松鎭雄
  • Organizer: フォーラム2008 : 衛生薬学・環境トキシコロジー
  • Place of Presentation: 熊本
  • Year and Date: 2008-10-18