The effect of temperature and morphology on insulin secreting signal and insulin secretion in insulin secreting cells

• Project/Area Number: 20590208
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General physiology
• Research Institution: Hamamatsu University School of Medicine
• Principal Investigator: MOGAMI Hideo Hamamatsu University School of Medicine, 医学部, 准教授 (90311604)
• Co-Investigator(Kenkyū-buntansha): TERAKAWA Susumu 浜松医科大学, 光量子医学センター, 教授 (50014246) ; SAKURAI Takashi 浜松医科大学, 光量子医学センター, 助教 (50283362)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: 膵β細胞 / 細胞内情報伝達 / インスリン / 生体温度 / 形態 / セカンドメッセンジャー / インスリン分泌 / TRPM2チャネル / 全反射蛍光顕微鏡 / カルシウムイオン / calcium ion / insulin secretion / TRPM2 channel / signal / transduction

Research Abstract

The effect of temperature and morphology on insulin secretion has been shown using either TRPM2 or Cx36 knockdown pseudoislets.

Research Products

• [Journal Article] In vivo imaging analysis of the interaction between unusually large von-Willebrand factor multimers and platelets on the surface of vascular wall (2011)
  • Author(s): Rybaltowski, Miroslaw, Suzuki, Yuko, Mogami, Hideo, Chlebinska, Iwona, Brzoska, Tomasz, Tanaka, Aki, Banno, Fumiaki, Miyata, Toshiyuki, Urano, Tetsumei
  • Journal Title: Pfluger Archives-Eur J Physiol Volume: in press
  • Peer Reviewed
• [Journal Article] A technique for monitoring multiple signals with a combination of prism-based total internal reflection fluorescence microscopy and epifluorescence microscopy. (2009)
  • Author(s): Eisuke Adachi, Yutaka Kazoe, Yohei Sato, Yuko Suzuki, Tetsumei Urano, Takehiko Ueyama, Naoaki Saito, Viacheslav O.Nikolaev, Martin J. Lohse, Makoto Tominaga, Hideo Moeami
  • Journal Title: Pfluger Archives-European Journal of Physiology 459 Pages: 227-234
  • Peer Reviewed
• [Journal Article] Sweet taste receptor expressed in pancreatic beta-cells activates the calcium and cyclic AMP signaling systems and stimulates insulin secretion. (2009)
  • Author(s): Nakagawa Y, Nagasawa M, Yamada S, Hara A, Moeami H, Nikolaev VO, Lohse, MJ, Shigemura N, Ninomiya, Y, Kojima I
  • Journal Title: PLoS ONE 4
  • Peer Reviewed
• [Journal Article] Unique secretory dynamics of tissue plasminogen activator and its modulation by plasminogen activator inhibitor-1 in vascular endothelial cells. (2009)
  • Author(s): Suzuki Y, Moeami H. Ihara H, Urano T
  • Journal Title: Blood 113 Pages: 470-478
  • Peer Reviewed
• [Journal Article] Unique secretory dynamics of tissue plasminogen activator and its modulation by plasminogen activator inhibitor-1 in vascular endothelial cells (2009)
  • Author(s): Suzuki Y
  • Journal Title: Blood 113 Pages: 470-78
  • Peer Reviewed
• [Journal Article] Sweet taste receptor expressed in pancreatic beta-cells activates the calcium and cyclic AMP signaling systems and stimulates insulin secretion (2009)
  • Author(s): Nakagawa Y
  • Journal Title: PLoS ONE 4
  • Peer Reviewed
• [Journal Article] A technique for monitoring multiple signals with a combination of prism-based total internal reflection fluorescence microscopy and epifluorescence microscopy (2009)
  • Author(s): Adachi E
  • Journal Title: Pfluger Archives-European Journal of Physiology 459 Pages: 227-234
  • Peer Reviewed
• [Journal Article] Unique secretory dynamics of tissue plasminogen activator and its modulation by plasminogen activator inhibitor-1 in vascular endothelial cells. (2009)
  • Author(s): Suzuki Y, Mogami H, Ihara H, Urano T
  • Journal Title: Blood 113 Pages: 470-478
  • NAID: 120005313162
  • Peer Reviewed
• [Journal Article] Real-time analysis of platelet aggregation and procoagulant activity during thrombus formation in vivo. (2008)
  • Author(s): Hayashi T, Moeami H, Murakami Y, Nakamura T, Kanayama N, Konno H, UranoT.
  • Journal Title: Pfluger Archives-Eur J Physiol. 456 Pages: 1239-1351
  • Peer Reviewed
• [Journal Article] Real-time analysis of platelet aggregation and procoagulant activity during thrombus formation in vivo. (2008)
  • Author(s): Hayashi T, Mogami H, Murakami Y, Nakamura T, Kanayama N, Konno H, Urano T.
  • Journal Title: Pfluger Archives-Eur J Physiol 456 Pages: 1239-1251
  • Peer Reviewed
• [Presentation] Secretagogue-induced multiple signal interconnections in insulin secreting cells. (2010)
  • Author(s): Moeami H
  • Organizer: 14^<th> International congress of endocrinlogy
  • Place of Presentation: Kyoto, Japan
• [Presentation] Secretagogue-induced multiple signal interconnections in insulin secreting cells.14th International congress of endocrinlogy (2010)
  • Author(s): Mogami H
  • Organizer: 14th International congress of endocrinlogy
  • Place of Presentation: 京都
• [Presentation] Secretagogue-induced multiple signal cross-talk in insulin secreting cells (2010)
  • Author(s): Mogami H
  • Organizer: 14^<th> International congress of physiological science
  • Place of Presentation: Kyoto, Japan
• [Presentation] Secretagogue-induced multiple signal interconnections in insulin secreting cells. (2009)
  • Author(s): Moeami H
  • Organizer: 36^<th> International congress of physiological science
  • Place of Presentation: Kyoto, Japan
• [Presentation] Secretagogue-induced multiple signal interconnections in insulin secreting cells (2009)
  • Author(s): Mogami H
  • Organizer: 36^<th> International congress of physiological science
  • Place of Presentation: Kyoto, Japan
• [Presentation] Real-time analysis of platelet aggregation and procoagulant activity during thrombus formation in vivo Frontiers of Biological Imaging (2008)
  • Author(s): Moeami H
  • Organizer: 39^<th> NIPS International Symposium & 7^<th> OIB Symposium
  • Place of Presentation: Okazaki, Japan
• [Book] 血栓止血誌(技術講座「生体内リアルタイムイメージングによる血栓形成過程の解析」) (2008)
  • Author(s): 浦野哲盟、鈴木優子、林忠毅、最上秀夫
• [Book] 血栓止血誌(血液及び血管内皮からみた血栓症リスク) (2008)
  • Author(s): 浦野哲盟、鈴木優子、井原勇人、最上秀夫
• [Remarks] ホームページ等