Cytoprotection against necrotic cell death by the stabilization of the lysosome
Project/Area Number |
20590211
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
General physiology
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Research Institution | Fukushima Medical University |
Principal Investigator |
HAZAMA Akihiro 福島県立医科大学, 医学部, 教授 (60218394)
|
Co-Investigator(Kenkyū-buntansha) |
KATSUDA Shin-ichiro 福島県立医科大学, 医学部, 准教授 (80285022)
MIYAKE Masao 福島県立医科大学, 医学部, 助教 (00381385)
KOBAYASHI Daisuke 福島県立医科大学, 医学部, 助教 (40528080)
|
Project Period (FY) |
2008 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
Keywords | 細胞死 / ネクローシス / 塩素イオン / リソソーム / 細胞傷害 / amphotericin B / 細胞保護 / イオンチャネル / 塩素 |
Research Abstract |
We visualized the lysosome of HeLa cells using pepstatine or lysosensor. The lysosomal signal disappeared by the necrotic cell damage. The replacement of Cl-by other anion or the application of Cl-channel blocker protected the lysosomal signal against the necrotic cell damage. These results indicate that the inhibition of Cl-fluxes has the cytoprotective effect via the stabilization of liposome.
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Report
(4 results)
Research Products
(10 results)
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[Journal Article] Plasma membrane aquaporin AqpZ protein is essential for glucose metabolism during photomixotrophic growth of Synechocystis2011
Author(s)
Akai M, Onai K, Kusano M, Sato M, Redestig H, Toyooka K, Morishita M, Miyake H, Hazama A, Checchetto V, Szabo I, Matsuoka K, Saito K, Yasui M, Ishiura M, Uozumi N.
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Journal Title
J Biol Chem
Volume: 15(286)
Pages: 25224-35
Related Report
Peer Reviewed
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[Journal Article] Improved islet yield and function by use of a chloride channel blocker during collagenase digestion2011
Author(s)
Anazawa T, Sato Y, Saito T, Tsuchiya T, Kenjo A, Kimura T, Haga J, Miyake M, Waguri S, Hazama A, Gotoh M.
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Journal Title
Transplantation
Volume: 27(92)
Pages: 871-7
Related Report
Peer Reviewed
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