Cytoprotection against necrotic cell death by the stabilization of the lysosome

• Project/Area Number: 20590211
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General physiology
• Research Institution: Fukushima Medical University
• Principal Investigator: HAZAMA Akihiro 福島県立医科大学, 医学部, 教授 (60218394)
• Co-Investigator(Kenkyū-buntansha): KATSUDA Shin-ichiro 福島県立医科大学, 医学部, 准教授 (80285022) ; MIYAKE Masao 福島県立医科大学, 医学部, 助教 (00381385) ; KOBAYASHI Daisuke 福島県立医科大学, 医学部, 助教 (40528080)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 細胞死 / ネクローシス / 塩素イオン / リソソーム / 細胞傷害 / amphotericin B / 細胞保護 / イオンチャネル / 塩素

Research Abstract

We visualized the lysosome of HeLa cells using pepstatine or lysosensor. The lysosomal signal disappeared by the necrotic cell damage. The replacement of Cl-by other anion or the application of Cl-channel blocker protected the lysosomal signal against the necrotic cell damage. These results indicate that the inhibition of Cl-fluxes has the cytoprotective effect via the stabilization of liposome.

Research Products

• [Journal Article] Plasma membrane aquaporin AqpZ protein is essential for glucose metabolism during photomixotrophic growth of Synechocystis (2011)
  • Author(s): Akai M, Onai K, Kusano M, Sato M, Redestig H, Toyooka K, Morishita M, Miyake H, Hazama A, Checchetto V, Szabo I, Matsuoka K, Saito K, Yasui M, Ishiura M, Uozumi N.
  • Journal Title: J Biol Chem Volume: 15(286) Pages: 25224-35
  • Peer Reviewed
• [Journal Article] Improved islet yield and function by use of a chloride channel blocker during collagenase digestion (2011)
  • Author(s): Anazawa T, Sato Y, Saito T, Tsuchiya T, Kenjo A, Kimura T, Haga J, Miyake M, Waguri S, Hazama A, Gotoh M.
  • Journal Title: Transplantation Volume: 27(92) Pages: 871-7
  • Peer Reviewed
• [Journal Article] A tissue-engineered trachea derived from a framed collagen scaffold, gingival fibroblasts and adipose-derived stem cells. (2010)
  • Author(s): Kobayashi K, Suzuki T, Nomoto Y, Tada Y, Miyake M, Hazama A, Wada I, Nakamura T, Omori K.
  • Journal Title: Biomaterials Volume: 31(18) Pages: 4855-63
  • Peer Reviewed
• [Journal Article] The effect of topical amiloride eye drops on tear quantity in rabbits (2010)
  • Author(s): Hara S, Hazama A, Miyake M, Kojima T, Sasaki Y, Shimazaki J, Dogru M, Tsubota K.
  • Journal Title: Mol Vis Volume: 4(16) Pages: 2279-85
  • Peer Reviewed
• [Journal Article] The effect of topical amiloride eye drops on tear quantity in rabbits (2010)
  • Author(s): Hara S, Hazama A, Miyake M, Kojima T, Sasaki Y, Shimazaki J, Dogru M, Tsubota K
  • Journal Title: Molecular Vision Volume: 16 Pages: 2279-2285
  • Peer Reviewed
• [Journal Article] A tissue-engineered trachea derived from a framed collagen scaffold, gingival fibroblasts and adipose-derived stem cells (2010)
  • Author(s): Kobayashi K, Suzuki T, Nomoto Y, Tada Y, Miyake M, Hazama A, Wada I, Nakamura T, Omori K
  • Journal Title: Biomaterials Volume: 18 Pages: 4855-4863
  • Peer Reviewed
• [Presentation] ウシ卵細胞における膜電位分布解析 (2012)
  • Author(s): 三宅将生、宮村元晴、濱野晴三、兼子智、挾間章博
  • Organizer: 日本生理学会大会
• [Presentation] セシウムは細胞増殖を抑制する (2012)
  • Author(s): 小林大輔、垣野内景、松村香楠、挾間章博
  • Organizer: 細胞統合生理日本生理学会大会
• [Presentation] リソソーム安定化機構と細胞死 (2011)
  • Author(s): 挾間章博, 三宅将生
  • Organizer: 福島医大・医・細胞統合生理日本生理学会大会
• [Presentation] アンフォテリシンB誘導性ネクローシスにおける細胞骨格の役割 (2010)
  • Author(s): 三宅将生、挾間章博
  • Organizer: 福島医大・医・細胞統合生理日本生理学会大会