Analysis of ATP-dependency of Na^+/Mg^<2+> exchange

• Project/Area Number: 20590219
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General physiology
• Research Institution: Tokyo Medical University
• Principal Investigator: YOKOYAMA Michiko Tokyo Medical University, 医学部, 講師 (20398762)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 生体膜 / チャネル / トランスポーター / 能動輸送 / ナトリウム・マグネシウム交換系 / アデノシン三燐酸 / ナトリウム・マグネシウム交換輸送阻害剤 / ナトリウム・カルシウム交換輸送阻害剤 / マグネシウムイオン

Research Abstract

We studied Na^+/Mg^2+ transport rate after metabolic inhibition in rat ventricular myocyte. A decrease in ATP below the threshold of rigor cross-bridge formation, rather than elevation of [Na^+]i or intracellular acidosis, inhibits the Mg^2+ efflu～90%), suggesting the absolute necessity of ATP for the Na6+/Mg^2+ exchange.

Research Products

• [Journal Article] KB-R7943 inhibits Na^+-dependent Mg^<2+> efflux in rat ventricular myocytes (2010)
  • Author(s): Michiko Tashiro, Hana Inoue, Masato Konishi
  • Journal Title: J Physiol Sci 60 Pages: 415-424
  • Peer Reviewed
• [Journal Article] KB-R7943 inhibits Na^+-dependent Mg^<2+> efflux in rat ventricular myocytes. (2010)
  • Author(s): Michiko Tashiro
  • Journal Title: Journal of Physiological Science Volume: 60 Pages: 415-424
  • Peer Reviewed
• [Journal Article] Metabolic inhibition strongly inhibits Na^+-dependent Mg^<2+> efflux in rat ventricular myocytes. (2009)
  • Author(s): Michiko Tashiro, Hana Inoue, Masato Konishi
  • Journal Title: Biophys J 96 Pages: 4941-4950
  • Peer Reviewed
• [Journal Article] Metabolic inhibition strongly inhibits Na^+-dependent Mg^<2+> efflux in rat ventricular myocytes. (2009)
  • Author(s): Michiko Tashiro
  • Journal Title: Biophysical Journal (In press)
  • Peer Reviewed
• [Presentation] ラット心室筋細胞におけるMg^<2+>輸送に対するMg^<2+>欠乏の影響 (2011)
  • Author(s): 田代倫子、井上華、小西真人
  • Organizer: 第88回日本生理学会
  • Place of Presentation: 誌上開催
  • Year and Date: 2011-03-29
• [Presentation] ラット心室筋細胞におけるMg^<2+>輸送に対するMg^<2+>欠乏の影響 (2011)
  • Author(s): 田代倫子
  • Organizer: 第88回日本生理学会
  • Place of Presentation: パシフィコ横浜・神奈川県(誌上開催)
  • Year and Date: 2011-03-29
• [Presentation] Na^+依存性Mg^<2+>汲みだし輸送活性に対するNa^+/Ca^<2+>交換輸送阻害薬の効果 (2010)
  • Author(s): 田代倫子、 井上華、小西真人
  • Organizer: 第87回日本生理学会盛岡市民文化ホール
  • Place of Presentation: 岩手県
  • Year and Date: 2010-05-20
• [Presentation] Na^+依存性Mg^<2+>汲みだし輸送活性に対するNa^+/Ca^<2+>交換輸送阻害薬の効果 (2010)
  • Author(s): 田代倫子
  • Organizer: 第87回日本生理学会
  • Place of Presentation: 盛岡市民文化ホール(岩手県)
  • Year and Date: 2010-05-20
• [Presentation] ATP requirement of the Na^+-dependent Mg^<2+> efflux in rat ventricular myocytes (2009)
  • Author(s): Michiko Tashiro
  • Organizer: 第36回国際生理学会(第86回日本生理学会)
  • Place of Presentation: 国立京都国際会館(京都府)
  • Year and Date: 2009-07-31
• [Presentation] Is ATP required for activities of the Na^+/Mg^<2+> exchange? (2009)
  • Author(s): Michiko Tashiro, Hana Inoue, Masato Konishi.
  • Organizer: 第53回アメリカ生物物理学会
  • Place of Presentation: ボストン(アメリカ合衆国マサチューセッツ州)
  • Year and Date: 2009-03-04
• [Presentation] Is ATP required for activities of the Na^+/Mg<2+> exchange? (2009)
  • Author(s): Michiko Tashiro
  • Organizer: Biophysical Society Annual Meeting
  • Place of Presentation: アメリカ合衆国ボストン
  • Year and Date: 2009-03-04