| Project/Area Number |
20590303
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Chiba University |
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Principal Investigator |
HATANO Masahiko Chiba University, 大学院・医学研究院, 教授 (20208523)
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| Co-Investigator(Kenkyū-buntansha) |
FUJIMURA Lisa 千葉大学, バイオメディカル研究センター, 助教 (30376363)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 神経堤細胞 / 転写抑制因子 / 腸管神経 / ノックアウトマウス / 細胞周期 / 腸管神経節 / 転写因子 / Ncx / Nczf / ヒルシュスプルング病類縁疾患 / 細胞周期制御 / コンディショナルKOマウス / 遺伝子 / 発生・分化 / 細胞・組織 / 疾患モデルマウス / 腸管神経細胞 |
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| Research Abstract |
Neural crest cells differentiate into diverse tissue types such as enteric neurons, sympathetic neurons, and melanocytes. Abnormal development of neural crest cells results in Hirschsprung's disease, neuroblastoma, and other anomaly syndrome in newborn. We have identified Ncx which is specifically expressed in neural crest derived cells and searched for its target genes. We found that Nczf, one of the target genes, controls cell proliferation during embryogenesis by negatively regulating p27 expression. Another target gene, Prickle 2, regulates neurite extention in neuroblastoma cell line. Analysis of Ncx target gene network will contribute to the development of new therapeutic methods of neurocristopathy by degenerative medicine using ES or iPS cells.
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