A study for the role of ADAMs in the relationship between hepatocellular carcinoma and hepatic stellate cells.

• Project/Area Number: 20590371
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Human pathology
• Research Institution: Iwate Medical University
• Principal Investigator: KOTANI Koji Iwate Medical University, 医学部, 助教 (50433488)
• Co-Investigator(Kenkyū-buntansha): MASUDA Tomoyuki 岩手医科大学, 医学部, 教授 (10199698) ; MAESAWA Chihaya 岩手医科大学, 医学部, 教授 (10326647) ; OIKAWA Hiroki 岩手医科大学, 医, 講師 (50285582)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2008: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 肝 / 星細胞 / 間質 / ADAM / 相互作用 / 肝臓 / 肝線維症 / 肝星細胞 / エピジェネティックス / 肝炎 / 肝硬変

Research Abstract

We have attempted to develop the model of relationship between hepatocellular carcinoma and hepatic stellate cells. For the purpose, we constructed two inducible clones (Tet-kADAM-HepG2 and Tet-kADAM-LI-90). However, both we failed to the cloning of both transfectans. We generated other clones using another system, co-culture markedly decrease tumor cell growth and invasion activity. Thus, the relationships between HSC and hepatocellular carcinoma might contribute to hepatocellular carcinoma development.

Research Products

• [Journal Article] Down-regulation of microRNA-211 is involved in expression of preferentially expressed antigen of melanoma (PRAME) in melanoma cells. (2011)
  • Author(s): E. Sakaurai, C Maesawa
  • Journal Title: Int J Oncol in press
• [Journal Article] Accumbens-Associated 1 Contributes to Cortactin Deacetylation and Augments the Migration of Melanoma Cells. (2011)
  • Author(s): K. Tsunoda, H. Oikawa, H. Tada, Y. Tatemichi, S. Muraoka, S. Miura, M. Shibazaki, F. Maeda, K. Takahashi, T. Akasaka, T. Masuda, C. Maesawa, Nucleus
  • Journal Title: J Invest Dermatol in press
• [Journal Article] Nucleus accumbens-associated 1 contributes to cortactin deacetylation and augments the migration of melanoma cells. (2011)
  • Author(s): Tsunoda K
  • Journal Title: J Invest Dermatol Volume: 131(In press)
  • Peer Reviewed
• [Journal Article] Immunohistochemical Study of HER2 and TUBB3 Proteins in Extramammary Paget Disease. (2011)
  • Author(s): Miyamoto A
  • Journal Title: Am J Dermatopathol Volume: 33(In press)
  • Peer Reviewed
• [Journal Article] Non-invasive evaluation of liver fibrosis using acoustic radiation force impulse imaging in chronic hepatitis patients with hepatitis C virus infection. (2010)
  • Author(s): H. Kuroda, K. Kakisaka, Y. Tatemichi, K. Sawara, Y. Miyamoto, K. Oikawa, A. Miyasaka, Y. Takikawa, T. Masuda, K. Suzuki
  • Journal Title: Hepatogastroenterology 57 Pages: 1203-1207
• [Journal Article] Detection of human papillomavirus in a urothelial carcinoma mimicking urethral caruncle. (2010)
  • Author(s): Y. Tatemichi, H. Oikawa, C. Maesawa, J. Ambo, M. Sato, H. Koike, T. Sata, T. Fujioka, T. Masuda
  • Journal Title: Int J Urol 17 Pages: 189-191
  • NAID: 10027257188
• [Journal Article] Association of the TNF-{alpha}-C-857T polymorphism with resistance to the cholesterol-lowering effect of HMG-CoA reductase inhibitors in type 2 diabetic subjects. (2010)
  • Author(s): T. Takahashi, K. Takahashi, M. Yamashina, C. Maesawa, T. Kajiwara, H. Taneichi, N. Takebe, Y. Kaneko, T. Masuda, J. Satoh
  • Journal Title: Diabetes Care 33 Pages: 463-466
• [Journal Article] Expression profiles of nestin in vascular smooth muscle cells in vivo and in vitro. (2010)
  • Author(s): H. Oikawa, K. Hayashi, C. Maesawa, T. Masuda, K. Sobue
  • Journal Title: Exp Cell Res 316 Pages: 940-950
• [Journal Article] Immunohistochemical Study of HER2 and TUBB3 Proteins in Extramammary Paget Disease. (2010)
  • Author(s): A. Miyamoto, K. Akasaka, H. Oikawa, T. Akasaka, T. Masuda, C. Maesawa
  • Journal Title: Am J Dermatopathol
• [Journal Article] Non-invasive evaluation of liver fibrosis using acoustic radiation force impulse imaging in chronic hepatitis patients with hepatitis C virus infection. (2010)
  • Author(s): Kuroda H
  • Journal Title: Hepatogastroenterology Volume: 57 Pages: 1203-1207
  • Peer Reviewed
• [Journal Article] Enhanced goblet cell hyperplasia in HDC knockout mice with allergic airway inflammation. (2009)
  • Author(s): K. Yamauchi, H.M. Piao, T. Nakadate, T. Shikanai, Y. Nakamura, H. Ito, T. Mouri, H. Kobayashi, C. Maesawa, T. Sawai, H. Ohtsu, H. Inoue
  • Journal Title: Allergol Int 58 Pages: 125-134
  • NAID: 10025109331
• [Journal Article] Spontaneous [Ca2+]i oscillations in G1/S phase-synchronized cells. (2009)
  • Author(s): A.D. Russa, C. Maesawa, Y. Satoh
  • Journal Title: J Electron Microsc (Tokyo) 58 Pages: 321-329
• [Journal Article] Ontogeny of reticular framework of white pulp and marginal zone in human spleen : immunohistochemical studies of fetal spleens from the 17th to 40th week of gestation. (2009)
  • Author(s): T. Satoh, E. Sakurai, H. Tada, T. Masuda
  • Journal Title: Cell Tissue Res 336 Pages: 287-297
• [Journal Article] Differential microRNA expression between bone marrow side population cells and hepatocytes in adult mice. (2009)
  • Author(s): Y. Nagata, C. Maesawa, H. Tada, Y. Takikawa, A. Yashima-Abo, T. Masuda
  • Journal Title: Int J Mol Med 24 Pages: 35-43
• [Journal Article] Effect of atorvastatin on microRNA 221 / 222 expression in endothelial progenitor cells obtained from patients with coronary artery disease. (2009)
  • Author(s): Y. Minami, M. Satoh, C. Maesawa, Y. Takahashi, T. Tabuchi, T. Itoh, M. Nakamura
  • Journal Title: Eur J Clin Invest 39 Pages: 359-367
• [Journal Article] DNA hypomethylation at the CpG island is involved in aberrant expression of the L1 cell adhesion molecule gene in colorectal cancer. (2009)
  • Author(s): K. Kato, C. Maesawa, T. Itabashi, K. Fujisawa, K. Otsuka, S. Kanno, H. Tada, Y. Tatemichi, K. Kotani, H. Oikawa, T. Sugai, G. Wakabayashi, T. Masuda
  • Journal Title: Int J Oncol 35 Pages: 467-476
• [Journal Article] Loss of class III beta-tubulin induced by histone deacetylation is associated with chemosensitivity to paclitaxel in malignant melanoma cells. (2009)
  • Author(s): K. Akasaka, C. Maesawa, M. Shibazaki, F. Maeda, K. Takahashi, T. Akasaka, T. Masuda
  • Journal Title: J Invest Dermatol 129 Pages: 1516-1526
• [Journal Article] DNA hypomethylation at the CpG island is involved in aberrant expression of the L1 cell adhesion molecule gene in colorectal cancer. (2009)
  • Author(s): Kato K, Maesawa C, Itabashi T, Fujisawa K, Otsuka K, Kanno S, Tada H, Tatemichi Y, Kotani K, Oikawa H, Sugai T, Wakabayashi G, Masuda T.
  • Journal Title: Int J Oncol 35 Pages: 467-476
  • Peer Reviewed
• [Journal Article] Downregulation of miR-138 is associated with overexpression of human telomerase reverse transcriptase protein in human anaplastic thyroid carcinoma cell lines. (2008)
  • Author(s): S. Mitomo, C. Maesawa, S. Ogasawara, T. Iwaya, M. Shibazaki, A. Yashima-Abo, K. Kotani, H. Oikawa, E. Sakurai, N. Izutsu, K. Kato, H. Komatsu, K. Ikeda, G. Wakabayashi, T. Masuda
  • Journal Title: Cancer Sci 99 Pages: 280-286
  • NAID: 10024002213
• [Journal Article] Epigenetic modification is involved in aberrant expression of class III beta-tubulin, TUBB3, in ovarian cancer cells. (2008)
  • Author(s): N. Izutsu, C. Maesawa, M. Shibazaki, H. Oikawa, T. Shoji, T. Sugiyama, T. Masuda
  • Journal Title: Int J Oncol 32 Pages: 1227-1235
• [Journal Article] Angiotensin II and epidermal growth factor receptor cross-talk mediated by a disintegrin and metalloprotease accelerates tumor cell proliferation of hepatocellular carcinoma cell lines. (2008)
  • Author(s): H. Itabashi, C. Maesawa, H. Oikawa, K. Kotani, E. Sakurai, K. Kato, H. Komatsu, H. Nitta, H. Kawamura, G. Wakabayashi, T. Masuda
  • Journal Title: Hepatol Res 38 Pages: 601-613
  • NAID: 10024148920
• [Presentation] 費と肝星細胞におけるAngiotensin IIによるEGFRの活性化とADAM17の関連の検討 (2008)
  • Author(s): 及川浩樹, 小谷康慈, 他
  • Organizer: 日本病理学会総会
  • Place of Presentation: 金沢
  • Year and Date: 2008-05-15
• [Remarks] ホームページ等